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All Journal Natural Science: Journal of Science and Technology Biocelebes KOVALEN: Jurnal Riset Kimia Jurnal Biologi Tropis Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Wahyu Harso
Jurusan Biologi, Fakultas MIPA Universitas Tadulako
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Immunology & microbiology

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Journal : Biocelebes

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UJI KEEFEKTIFAN EKSTRAK ALELOPATI AKAR TEKI (Cyperus rotundus L.) DAN BANDOTAN (Ageratum conyzoides L.) TERHADAP PERTUMBUHAN SAWI HIJAU (Brassica rapa L.) Sri Rahayu; Asri Pirade Paserang; Wahyu Harso
Biocelebes Vol. 14 No. 1 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i1.15083

Abstract

Weeds such as Cyperus rotundus L. dan Ageratum conyzoides L. release allelopatic compounds which can inhibit the growth of crops. The aim of this study was to compare the inhibition of allelopatic compounds contained C. rotundus L. and A. conyzoides root exctract to Brassica rapa L. growth. This study was conducted in a greenhouse and was based on Completely Randomized Design with two factors. The first factor was type of root extracts (extract from either C. rotundus root or A. conyzoides root) and the second factor was concentration of root extract. Root extract concentrations added to growth medium of B. rapa L. 10 days after planting were 0, 100, 300 and 500 g/L respectifely. The addition of root extract was repeated every week until crop harvested. The results showed that there was not significantly different between C. rotundus and A. conyzoides roots extract to inhibit B. rapa growth. Increassing of root extract concentrations from both roots increased inhibition of B. rapa growth.
PENGARUH INTENSITAS CAHAYA MATAHARI DANKETERSEDIAAN AIR TERHADAP PERTUMBUHANTANAMAN CABAI RAWIT (Capsicum frutescens L.) Ajis Ajis; Wahyu Harso
Biocelebes Vol. 14 No. 1 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i1.15084

Abstract

Light intensity is one the most important factor to the growth of Capsicum frutescens L, however high light intensity can cause high evaporation which causes plant suffering from drought. The aim of this study was to determine the effect of different sunlight intensities and soilwater availabity to C. frutescens growth. The study was conducted by a completely randomized design method with two factors. The first factor was light entensity which consisted of 100 and 50% sunlight intensity (4287 and 2587 lux). The second factor was soil water avaibility which consisted of 75, 50 and 25% fied capacity. The results showed that plants grown at 100% light intensity had higher growth than plants grown at 50% light intensity on every soil water availability treatments. Soil water availability was not significantly affecting plant growth. It might be caused by plant received short period of sunlight.
KAJIAN AUTEKOLOGI Areca vestiaria Giseke PADA HUTAN PEGUNUNGAN BAWAH NGATA TORO KAWASAN TAMAN NASIONAL LORE LINDU SULAWESI TENGAH Andi Wirdani Pettalolo; Wahyu Harso; Ramadanil Pitopang
Biocelebes Vol. 14 No. 2 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i2.15265

Abstract

The research entitled Autecolgical study of Areca vestaria in Sub-Montane Forest Lore Lindu National Park at Toro village area has been conducted from April to May 2016. The objective of the research was to know the biotics and abiotics factor arround the habitat of Areca vestiaria. The results showed that The highest important value index (IVI) of tree, sapling, pole and seedling species wereFicus ampelos (68.69%), Aphanaximis polistachy (40.01%), Ardisia lateriflora (31.73%) and Ardisia lateriflora (19.23%) respectively. The insects actively interacted with A. vestaria were black ant and bee. Based on soil analyzed indicated that pH of soil 6.6 while N, P and organic matter concentration were 0.26, 0.207 mg/g and 76.1% respectively. A. vestaria grew under light intensity of 540.4 lux, humidity of 76.1% and average daily temperature of 24.4%.
PERTUMBUHAN BAWANG MERAH (Allium cepa L.) YANG DIBERI PUPUK KOMPOS CAIR DAN JAMUR MIKORIZA ARBUSKULAR Riska Palesa; Wahyu Harso
Biocelebes Vol. 14 No. 2 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i2.15267

Abstract

The application of liquid compost and of beneficial soil microorganism such as arbsucular mycorrhizal fungi can be used to replace chemical fertilizer application. The aim of this study was to investigate the growth of red onion (Allium cepa L.) plant fertilized by liquid compost and inoculated by arbuscular mycorrhizal fungi. This study was conducted based on a completely randomized design with two factors. The first factor was an addition of liquid compost dosages (0, 50, 100 and 200 ml/polybag). The second factor was an addition of AM fungal inoculum (with and without addition). The results showed that the growth of red onion plant was not significantly affected by the addition of liquid compost and AM fungal inoculum. However, the red onion plant fertilized by 200 ml liquid compost per polybag had the lowest shoot dry weight. The addition of AM fungal inoculum was not increasing plant growth because the quality of used inoculum was not good.
EFEKTIVITAS Glomus clorum TERHADAP PERTUMBUHAN TANAMAN CABAI RAWIT (Capsicum frutescens L.), TOMAT (Solanum lycopersicum L.) DAN TERUNG (Solanum melongena L.) Khairun Nisa; Yusran; Wahyu Harso
Biocelebes Vol. 14 No. 2 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i2.15271

Abstract

Increasing plant growth by arbuscula mycorrhizal fungi depends on the fungus and plant spesies. The aim of this study was to determine the efectivity of fungal mycorrhizal fungus on the growth of cayenne pepper, tomato and eggplant which are Solanaceae family members. The study was conducted by Completely Randomized Design method.with two factors. The fisrt factor was species of experimental plant consisted of cayenne pepper (Capsicum frutescens L.), tomato (Solanum lycopersicum L.) and eggplant (Solanum melongena L.). The second factor was addition of Glomus clorum inokulum consisted of with and without inoculum addition. The result showed that there was no effect of inoculum addition on plant growth of three experiemal plants. The quality of inoculum used for this experiment was not good enough furthermore fungi could not germinate and colonize plant root.
PENGARUH PUPUK HAYATI DENGAN KOMPOSISI MIKROORGANISME YANG BERBEDA TERHADAP PERTUMBUHAN TANAMAN Amalia Puji` Cahyani; Asri Pirade Paserang; Meryany Ananda; Wahyu Harso
Biocelebes Vol. 15 No. 2 (2021)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v15i2.15781

Abstract

Biofertilizer can be used to replace chemical fertilizer so that soil quality is maintained and soil pollution can be avoided. The study aimed to determine the effect of biofertilizers with different compositions of microorganisms on the growth of maize plants. The research was carried out in a greenhouse. Maize plants were fertilized by two kinds of biofertilizers with different compositions of microorganisms. The first biofertilizer contained Azotobacter sp, Azospirillum sp, Rhizobium sp, Trichoderma sp, and Lactobacillus sp, and the second biofertilizer contained Strenotrophomonas sp. and Paenibacillus polymyxa. As controls, maize plants were fertilized by sterilized those biofertilizers. The results showed that the second biofertilizer increased the dry weight of the maize plant. Meanwhile, the first biofertilizer did not increase it. Several things must be considered in the application of biofertilizers, such as the composition of microorganisms, type of plant, level of fertilization, and the method of applying fertilizer to the plant.
FITOKIMIA, DAN AKTIFITAS ANTIBAKTERI DARI Etlingera sublimata Poulsen (ZINGIBERACEAE), TUMBUHAN ENDEMIK SULAWESI: Phytochemical and Antibacterial Activity of Etlingera sublimata Poulsen (Zingiberaceae), Endemic Plant to Sulawesi Ramadanil Pitopang; Endang Lestari; PUTI ANDALUSIA SARIGANDO Banilai; Wahyu Harso
Biocelebes Vol. 16 No. 2 (2022)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v16i2.15957

Abstract

The research entitled "Phytochemical Screening and antibacterial activity of Etlingera sublimata Poulsen (Zingiberaceae), an Endemic Sulawesi of Plants was conducted from January 2020 to June 2020, at the Plant Biosystematics Laboratory and Biotechnology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Tadulako University. Samples of E. sublimata were collected from the mountane forests of the Lore Lindu National Park (LLNP), arround Sedoa village, Lore Utara District, Poso Regency The objectives of the research were to analyze the secondary metabolite compounds in E. sublimata plants and to determine the antibacterial activity of E. sublimata leaves extract in againts Salmonella thypii bacteria. Plant samples were extracted by maceration methods with 96% ethanol as a solvent. Actibacterial activity was tested by using agar well diffusion methods. The experiment was designed by Completely Randomized Design (CRD) with seven different concentration of E. sublimata extract (15, 25, 50, 75 and 85%). Extract and standard drugs were prepared in double-distilated water using Nutrient Agar tubes. 2% Chloramphenicol as standard drugs was used as a positive control and sterile aquadest was used as a negative control. The inhibition zone of bacteria and yeast growth around the disk was measured after 18 to 24 h incubation at 37°C. The results showed that the stem contains alkaloids, flavonoids, terpenoids, tannins and saponins. The rhizome containts alkaloid, flavonoids, terpenoids, tannins and saponins, the flowers containts alkaloid, terpenoids and tannins, while the leaves containts tannin and saponin compounds. Leaves extract of E.sublimata has inhibition activity on the cell growth a pathogenic Salmonella thypi bacteria that the effective extract concentration was 50% with an average inhibition zone ​​2.67 mm.
PERTUMBUHAN TANAMAN BAYAM AKAR MERAH (AMARANTHUS RETROFLEXUS) YANG DIINOKULASI DENGAN BERBAGAI JENIS JAMUR MIKORIZA ARBUSKULA YANG DITUMBUHKAN PADA TANAH TERCEMAR MERKURI Wahyu Harso; Devan Prima
Biocelebes Vol. 16 No. 2 (2022)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v16i2.16249

Abstract

Bayam akar merah (Amaranthus retroflexus) mampu tumbuh pada tanah tercemar logam berat tanpa menunjukkan symptom. Jamur mikoriza dapat meningkatkan pertumbuhan tanaman pada tanah tercemar logam berat. Tujuan penelitian ini yaitu mengetahui pengaruh pemberian jamur mikoriza arbuskular dari jenis yang berbeda terhadap pertumbuhan tanaman bayam pada tanah tercemar merkuri. Pada penelitian ini tanaman bayam diinokulasi dengan inokulum tunggal dari jenis Glomus sp., Acaulospora tuberculata, dan inokulum campuran dari jenis Acaulospora, Glomus, Gigaspora, dan Scutelospora dan tanpa pemberian inokulum sebagai kontrol. Tanaman bayam ditumbuhkan pada tanah yang tidak dicemari dan dicemari dengan logam merkuri sebanyak 8 mg HgCl/kg tanah. Hasil penelitian menunjukkan bahwa pertumbuhan tanaman bayam tidak dipengaruhi oleh tanah yang tercemar merkuri. Pemberian inokulum jamur mikoriza arbuskula cenderung menurunkan pertumbuhan tanaman. Tidak ada perbedaan yang nyata pada jenis jamur yang diberikan dalam menurunkan pertumbuhan tanaman bayam.
PERBANDINGAN JUMLAH BAKTERI TANAH PADA HUTAN BERDAUN LEBAR DAN HUTAN BERDAUN JARUM PADA KEDALAMAN TANAH YANG BERBEDA Abi Gael Sidon; Wahyu Harso; Umrah Umrah; Orryani Lambui
Biocelebes Vol. 17 No. 1 (2023)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v17i1.16439

Abstract

The number of soil microorganisms that play a role in the organic matter decomposition process can be affected by litter quantity and quality. Lignin content is higher in needleleaf than in broadleaf. The study aimed to determine the number of bacteria found in the broadleaf forest and needleleaf forest soil at different soil depths. The soil was collected at depths of 0-5 cm, 10-15 cm, and 20-25 cm at a distance of 1.5 m from the canopy of both broadleaf and coniferous forests where their locations are close. The results showed that the soil organic matter of broadleaf forests was higher than needleleaf forests, especially at depths of 0-5 cm and 10-15 cm. The number of bacteria on broadleaf forest soil is higher than on needleleaf forest soil, especially at 0-5 cm depth. The organic matter in both forest soils decreases with the soil depth. A positive correlation is obtained between the quantity of organic matter and the number of bacteria. The quantity and quality of organic matter in the soil will affect the number of bacteria.
APLIKASI KOMPOS CAIR AEROB DAN ANAEROB PADA PERTUMBUHAN TANAMAN JAGUNG: Application of Aerobic and Anaerobic Liquid Compos on Corn Plant Growth Wasilahturahman; Harso, Wahyu; Umrah; Ananda, Meryany
Biocelebes Vol. 17 No. 2 (2023)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v17i2.16611

Abstract

The price of chemical fertilizers and potential environmental risks are the reasons for using compost. Liquid compost extracted from compost has many advantages that a compost. Liquid compost is available quickly and has a higher concentration compared to compost. The effectiveness of liquid compost depends on the aeration treated in the production process. The study aimed to determine the growth of corn plants fed with either aerated liquid compost or an aerated liquid compost. This study was conducted by a Complete Randomized Design with two factors. The first factor is the type of liquid compost, aerobic and anaerobic liquid compost. The second factor is the dose of a liquid compost, 100 ml and 200 ml of liquid compost. The growth medium was drenched with aerobic or anaerobic liquid compost in the first and third weeks after planting. The results showed that plants fertilized by aerobic or anaerobic liquid compost increased plant growth. The plant fertilized by anaerobic liquid compost had higher plant growth than it fertilized by aerobic liquid compost. Key words: aerobic, anaerobic, corn plants, liquid compost Harga pupuk kimia dan potensi resiko lingkungan merupakan alasan penggunan kompos. Kompos cair merupakan ekstrak dari kompos memiliki keuntungan yang tidak dimiliki oleh kompos yaitu tersedia dengan cepat dan memiliki konsentrasi yang lebih tinggi dibandingkan dengan kompos. Efektifitas kompos cair tergantung dari aerasi yang diberikan pada proses pembuatannya. Tujuan dari penelitian ini adalah untuk mengetahui pertumbuhan tanaman jagung yang diberi kompos cair yang dibuat secara aerob dan anaerob. Penelitian dilakukan dengan menggunakan Rancangan Acal Lengkap dengan dua faktor. Faktor pertama adalah jenis kompos cair yaitu kompos cair aerob dan anaerob. Faktor kedua adalah dosis pemberian kompos cair yaitu 100 ml dan 200 ml. Media tanam tanaman jagung diberi kompos cair aerob atau anaerob di minggu pertama dan ketiga setelah tanam. Hasil penelitian menunjukkan bahwa pemberian kompos cair aerob atau anaerob meningkatkan pertumbuhan tanaman. Peningkatan pertumbuhan tanaman lebih tinggi terjadi pada tanaman yang diberi kompos cair anaerob dibandingkan dengan yang diberi kompos cair anaerob. Kata kunci : aerob, anaerob, pupuk cair, tanaman jagung
Co-Authors Abi Gael Sidon Agustinur Agustinur Ajis Ajis Amalia Puji` Cahyani Andi Wirdani Pettalolo Andre Andre, Andre Asri Pirade Paserang Devan Prima Dewi Arini Endang Lestari Givani Oktaviana Gobel, Sri Amaliah Imanuel , Natasya Nadya Iqbal, Moh isna isna Khairun Nisa Magfirahtul Jannah Melsi Mengkido Meryany Ananda Moh Iqbal Moh. Iqbal, Moh. Mustafid Rasyiid Mutmainnah Mutmainnah Orryani Lambui Paserang, Asri Prismawiryanti Prismawiryanti PUTI ANDALUSIA SARIGANDO Banilai Ramadanil Pitopang Retno Sari Riska Palesa Risma Risma Rudy, Sri Utami Satria Dhika Saputra Sitti Ayu Sri Rahayu Suwitra, I Ketut Tagentju, Indra A. Umrah Umrah Umrah Umrah Wasilahturahman Yayang Istikana Yusran Yusran Yusran Yusran