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A Preliminary Study of Ocimum basilicum Essential Oil as a Repellent against Aedes aegypti in Surabaya Wahyuni, Afrinda Dwi; Zahra, Siti Fatimatuz; Putri, Balqis Qonita; Pramudya, Manikya; Rohmah, Etik Ainun; Mulyatno, Kris Cahyo
Journal of Public Health for Tropical and Coastal Region Vol 5, No 2 (2022): Journal of Public Health for Tropical and Coastal Region
Publisher : Faculty of Public Health, Universitas Diponegoro, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jphtcr.v5i2.13734

Abstract

Introduction: Aedes aegypti is the main dengue vector in Indonesia. One of the dengue controls is through insecticides use. The plant-based insecticide is one alternative that can be used to minimize the impact of chemical insecticides. Ocimum basilicum is one of the plants that have the potency to be used as insecticides. The aims of this study were to analyze the insecticide activity of Ocimum basilicum essential oil against Aedes aegypti as well as its Lethal Concentration (LC50 and LC90) and Knockdown Time ( KT50 and KT90).Methods: Treatment groups were given Ocimum basilicum essential oil of 100 ppm, 50 ppm, and 25 ppm. The tests were performed four times with 10 mosquitoes per test.Results: The mortality rate of the mosquito on the extract with a concentration of 100 ppm was 100%, 50 ppm was 10% and 25 ppm was 0% and the value of LC50 = 62.9 ppm and LC90 = 78.7 ppm. KT50 of this study was 19.4 minutes and KT90 was 8.07 minutes.Conclusion: Ocimum basilicum essential oil has a mosquitocidal effect on Aedes aegypti. Further studies to investigate the substances of Ocimum basilicum against Aedes aegypti are needed.
Pelatihan bagi Kelompok Pemanfaat dan Pemelihara (KPP) Instalasi Pengolahan Air Limbah (IPAL) di Kabupaten Gresik Marhendra, Bara Awanda; Soedjono, Eddy Setiadi; Slamet, Agus; Masduqi, Ali; Marsono, Bowo Djoko; Yuniarto, Adhi; Purnomo, Alfan; Nurhayati, Ervin; Amalludin, Fahmi Ikhlasul; Wahyuni, Afrinda Dwi; Arliyani, Isni
Sewagati Vol 9 No 2 (2025)
Publisher : Pusat Publikasi ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j26139960.v9i2.2597

Abstract

Pengelolaan air limbah yang efektif sangat penting untuk meningkatkan kualitas hidup masyarakat dan menjaga kelestarian lingkungan. Untuk mendukung hal ini, Tim Pengabdian Masyarakat (Abmas) dari Laboratorium Teknologi Pengolahan Air, Departemen Teknik Lingkungan, Institut Teknologi Sepuluh Nopember (ITS), bekerja sama dengan Dinas Cipta Karya Kabupaten Gresik, mengadakan pelatihan bagi Kelompok Pemanfaat dan Pemelihara (KPP) Instalasi Pengolahan Air Limbah (IPAL). Pelatihan ini mencakup penyampaian materi, diskusi, dan praktik langsung terkait sistem pengelolaan limbah domestik, pemanfaatan hasil olahan, serta perawatan IPAL komunal. Hasil pelatihan menunjukkan peningkatan pemahaman peserta, yang diukur melalui sesi diskusi. Sebanyak 40 peserta menunjukkan antusiasme tinggi selama kegiatan. Program ini berkontribusi pada peningkatan akses sanitasi yang aman di Kabupaten Gresik, sekaligus mendukung pencitraan daerah sebagai wilayah yang peduli lingkungan. Kesimpulannya, pelatihan ini berhasil memberikan pengetahuan dan keterampilan yang relevan kepada peserta, sehingga dapat menjadi model bagi daerah lain dalam mengelola air limbah secara berkelanjutan.
Pelatihan Pengelolaan Air Limbah Domestik Bagi Developer Perumahan Di Kota Surabaya Yuniarto, Adhi; Soedjono, Eddy Setiadi; Slamet, Agus; Masduqi, Ali; Marsono, Bowo Djoko; Purnomo, Alfan; Nurhayati, Ervin; Marhendra, Bara Awanda; Amalludin, Fahmi Ikhlasul; Wahyuni, Afrinda Dwi; Arliyani, Isni
Sewagati Vol 9 No 2 (2025)
Publisher : Pusat Publikasi ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j26139960.v9i2.2613

Abstract

Permasalahan sanitasi, khususnya pengelolaan Sistem Pengelolaan Air Limbah Domestik Terpusat (SPALD-T), menjadi isu penting di Indonesia. Tantangan utama mencakup kurangnya pengetahuan, keterampilan, dan pemahaman pengelola SPALD-T, yang menyebabkan pengelolaan air limbah belum optimal. Dampaknya termasuk penyebaran bau dan risiko penyumbatan. Untuk mengatasi hal ini, diadakan pelatihan dan edukasi bagi pengelola SPALD-T di Surabaya. Pelatihan ini bertujuan meningkatkan pemahaman tentang pengelolaan air limbah, prinsip operasional SPALD-T, metode pemeliharaan, serta langkah pemantauan. Melalui pelatihan ini, peserta diharapkan memperoleh pengetahuan lebih baik dan keterampilan praktis yang mumpuni, sehingga mampu mengelola air limbah secara optimal. Hasilnya, kinerja SPALD-T dapat ditingkatkan, dengan mengurangi masalah bau dan penyumbatan serta mendukung keberlanjutan sistem pengelolaan. Program ini juga mendukung pencapaian target pemerintah dalam menyediakan akses sanitasi layak dan aman. Dengan pengelolaan yang lebih efektif, manfaat sanitasi yang lebih baik dapat dirasakan oleh masyarakat.
METABOLIC REGULATION AND EPIGENETIC CONTROL: UNRAVELING THE COMPLEXITY OF SKELETAL STEM CELL FATE AND BONE HEALTH Wahyuni, Afrinda Dwi
Journal of Stem Cell Research and Tissue Engineering Vol. 8 No. 1 (2024): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jscrte.v8i1.58141

Abstract

Skeletal stem cells (SSCs) are essential for bone formation and tissue regeneration within the skeletal system. These self-regenerating cells differentiate into various skeletal cell types, maintaining skeletal health. However, aging diminishes SSC capacity, impacting skeletal integrity. Epigenetics, the study of heritable changes in gene expression, plays a crucial role in stem cell regulation. Mechanisms like DNA methylation and histone modifications control gene expression without altering the DNA sequence. Dysregulation of epigenetic processes in transplanted cells may lead to immunological rejection or functional impairment. Understanding epigenetic regulation in stem cells is vital for tissue regeneration strategies. This narrative review focuses on summarizing existing scientific literature on epigenetic regulation within stem cells, particularly skeletal stem cells. The study utilized Google Scholar to search for relevant articles using keywords like "epigenetic", "stem cell", and "skeletal stem cell". Selection criteria included publication year, article title, abstract, Scopus ranking, and accessibility. Four articles were chosen as reference sources for the review. Recent research emphasizes cellular metabolism's role in regulating skeletal functions through skeletal stem cells (SSCs), crucial for skeletal health and potential regenerative therapies. Transcriptomic and epigenetic analysis of human SSCs reveal species-specific pathways. Metabolic pathways are vital for SSC selfrenewal and multipotency, with glycolysis being the primary energy source for human bone marrow stem cells. Aging affects bone cells and inherited epigenetic changes significantly influence cell fate. Recent studies identify Ptip as a key epigenetic regulator of glycolysis in SSCs, impacting growth plate activity.
Effect of Floating Plants on Constructed Wetland Microbial Fuel Cell Treating Domestic Wastewater Wahyuni, Afrinda Dwi; Yuniarto, Adhi; Arliyani, Isni; Min, Booki
Jurnal Presipitasi : Media Komunikasi dan Pengembangan Teknik Lingkungan Vol 22, No 3 (2025): November 2025
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/presipitasi.v22i3.980-992

Abstract

Sustainable domestic wastewater treatment is urgently needed amid water scarcity and rising energy demands. The Constructed Wetland–Microbial Fuel Cell (CW-MFC) system integrates phytoremediation and bioelectricity production by electroactive microorganisms, yet previous studies rarely compared the synergistic effects of different plant species within this system, limiting its optimization. This study evaluated CW-MFC performance using three plant configurations (Pistia stratiotes, Eichhornia crassipes, and their combination) integrated with Lactobacillus plantarum at two concentrations (2×10⁸ CFU/mL and 5×10⁸ CFU/mL). The reactors were operated for 18 days under identical hydraulic conditions to assess BOD, COD, ammonia, and TSS removal efficiencies and power density generation. Results showed that Eichhornia crassipes achieved the highest pollutant removal efficiencies with COD reduction up to 82%, while the system with 2×10⁸ CFU/mL bacterial concentration produced the highest power density of approximately 1032 mW/m². Interestingly, lower bacterial concentrations yielded higher power outputs, possibly due to reduced microbial competition for electron transfer sites, enhancing electroactive bacteria performance. In conclusion, integrating Eichhornia crassipes with L. plantarum at 2×10⁸ CFU/mL optimizes both pollutant removal and bioelectricity production, confirming CW-MFC as an environmentally friendly technology with potential for sustainable wastewater treatment and renewable energy generation.