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All Journal Jurnal Ilmu Pertanian BIOEDUSCIENCE Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Jurnal Agrotek Tropika jurnal Ilmu Pertanian (Agricultural Science) Jurnal Pembelajaran dan Biologi Nukleus Nusantara Science and Technology Proceedings Journal of Applied Plant Technology Agrocentrum Jurnal Bioteknologi & Biosains Indonesia
Lestari, Safira Rizka
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology

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DAMPAK KOMBINASI JENIS TANAH, KOMPOS DAN Trichoderma sp. TERHADAP KERAPATAN SPORA Trichoderma sp. Subhan, Aprellia Sofiatul; Arifin, Moch.; Wijayanti, Fitri; Maroeto, Maroeto; Lestari, Safira Rizka
Jurnal Agrotek Tropika Vol. 13 No. 1 (2025): JURNAL AGROTEK TROPIKA VOL 13, FEBRUARI 2025
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v13i1.8626

Abstract

Trichoderma sp. merupakan jamur yang dapat berperan sebagai agen pengendali hayati karena dapat memarasit jamur lainnya dan mempercepat dekomposisi bahan organik. Penelitian ini bertujuan untuk melihat proses dekomposisi bahan organik pada berbagai jenis tanah dengan bantuan Trichoderma sp. sebagai dekomposer. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) faktorial yang terdiri dari tiga faktor. Faktor pertama yaitu macam jenis tanah J1: Alfisol; J2: Inceptisol; dan J3: Vertisol. Faktor kedua yaitu dosis kompos K0: 0 ton/ha (0 gr/4,4kg tanah); K1: 10 ton/ha (15 gr/4,4 kg tanah); K2: 20 ton/ha (30 gr/4,4 kg tanah); dan K3: 30 ton/ha (45 gr/4,4 kg tanah). Faktor ketiga yaitu taraf pemberian Trichoderma sp. T1: 15 ml dari 106 spora/ml; T2: 15 ml dari 108 spora/ml; dan T3: 15 ml dari 1010 spora/ml. Hasil penelitian yang didapat pada perlakuan Jenis tanah, Kompos dan Trichoderma sp. tidak memberikan interaksi yang nyata terhadap kerapatan spora Trichoderma sp. pada perlakuan jenis tanah dan dosis kompos memberikan pengaruh nyata terhadap kerapatan spora Trichoderma sp. Pada jenis tanah Inceptisol memberikan nilai tertinggi di semua interval pengamatan kerapatan spora Trichoderma sp. dengan dosis kompos 20 ton/ha dan 30 ton/ha pada 14 Hari Setelah Inkubasi (HSI), 30 ton/ha pada 28 Hari Setelah Inkubasi (HSI) hingga 42 Hari Setelah Inkubasi (HSI) dan 20 ton/ha pada 56 Hari Setelah Inkubasi (HSI).
Effectiveness of Endophytic Bacteria Rhizophora sp. from Mangrove Plants to Control Root-Knot Nematodes (Meloidogyne spp.). Khasanah, Nurdiah; Lestari, Safira Rizka; Megasari, Dita
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 14 No. 3 (2025): June 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v14i3.1110-1117

Abstract

Meloidogyne spp. is a root-knot nematode that can cause damage to plants up to 68%. The common control is the use of pesticides, but in the long term, it can damage the soil, so it is necessary to use biological agents that can control Meloidogyne spp. without causing environmental damage. The purpose of this study was to determine the ability of endophytic bacteria to control root-knot nematodes Meloidogyne spp.. The method used in this study was the exploration of endophytic bacteria from the Wonorejo mangrove ecotourism area, Surabaya using purposive sampling method. The five best endophytic bacteria were tested further with antagonistic tests. This test was carried out in vitro using 3 concentrations, namely 106, 108, and 1010 CFU/ml with 3 repetitions. The obtained results were tested using probit analysis to obtain the LC50 values. It was found that LC50 values for the most effective concentration to kill 50% of the Meloidogyne sp population is 109 and 1010 CFU/ml. It was also revealed that the isolate that is most capable of controlling Meloidogyne spp. nematodes is isolate A43. Keywords: Antagonist test, Endhopic bacteria, LC50, Mangrove, Meloidogyne spp. 
EVALUATING THE LONG-TERM STORAGE TIME VIABILITY AND SIZE DYNAMICS OF BACILLUS SP. BIOENCAPSULATION IN SODIUM ALGINATE MATRIX Saputra, Mochammad Mirza; Wuryandari, Yenny; Rahmadhini, Noni; Lestari, Safira Rizka
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2549

Abstract

The use of biological agents such as Bacillus sp. bacteria has begun to be widely used by farmers as a new form of control. Bacillus sp. needs to require special methods in its application because it has certain living conditions, and Bacillus sp. is vulnerable to environmental pressures. Bioencapsulated formulation in the form of granules is considered effective as a form of bacterial application in soil because it is able to protect Bacillus sp. and maintain survival. This study aims to determine the best concentration of sodium alginate in maintaining the viability of Bacillus sp. at the in vitro stage and to see changes in the size of the beads. The beads were made using extrusion method by combining Bacillus sp. suspension and sodium alginate suspension at 1%, 1.5%, and 2% concentration. The concentration of sodium alginate was not a major factor in changes in bead size and viability test results experienced significant changes in each observation. 1.5% and 2% concentrations can reach the highest viability value of 3x106 CFU/mL and decreased during 1 month of observation. Changes in bead size and viability were caused by the alginase enzyme produced by Bacillus sp.
Co-Authors Anjani, Ajeng Distya Arika Purnawati Dita Megasari Fatimah Azzahra Fitri Wijayanti Hery Nirwanto, Hery Khasanah, Nurdiah Maghfiroh, Ainur Risma Miftakhul Maroeto Maroeto Moch. Arifin Noni Rahmadhini Nugraha, Rafi Dwi Nur Hidayat Penta SURYAMINARSIH Prasetyawati, Endang Triwahyu Saputra, Mochammad Mirza Sari, Zulfa Novita Sayekti, Ninik Ari Subhan, Aprellia Sofiatul Yenny WURYANDARI Zahra, Nabila