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All Journal Jurnal Ilmiah Pertanian JURNAL BUDIDAYA PERTANIAN Unri Conference Series: Community Engagement
Saputra, Rachmad
Agrotechnology Department, Agriculture Faculty, Universitas Riau, Indonesia
Agriculture, Biological Sciences & Forestry Humanities

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Kemampuan Penghambatan Formulasi Biofungisida Tepung Berbahan Aktif Trichoderma pseudokoningii Rifai Setelah Penyimpanan Terhadap Jamur Ganoderma boninense Pat. secara in vitro Rachmad Saputra; Yetti Elfina; Muhammad Ali
JURNAL BUDIDAYA PERTANIAN Vol 15 No 2 (2019): Jurnal Budidaya Pertanian
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/jbdp.2019.15.2.106

Abstract

Trichoderma pseudokoningii fungus has been tested as biological control agents against several pathogenic fungi, including the Ganoderma boninense fungus which causes basal stem rot disease on oil palm. However, in-field testing, the efficacy of antagonistic microbes still showed unstable results. Therefore, it is necessary to package antagonistic microbes in the form of formulations. This study aimed to determine the effect of storage time of flour biofungicide formulations on the viability and antagonism of T. pseudokoningii Rifai in inhibiting the G. boninense pathogenic fungus in vitro. The study was arranged in a Completely Randomized Design with 4 treatments: bagasse (F1), rice husks (F2), dregs (F4) and combinations of bagasse, rice husks and dregs (F12), with three replications. The results showed that the powder biofungicide formulation of F2 (rice husk) was able to maintain the viability of the T. pseudokoningii fungus as indicated by the high number of conidia and high growth rate compared to the other formulations. The T. pseudokoningii fungus in F2 formulation also showed the highest inhibition compared to other formulations after storage for 1 and 2 months. Keywords: biofungicide, formulation, Ganoderma boninense, Trichoderma pseudokoningii ABSTRAK Jamur Trichoderma pseudokoningii telah banyak diuji sebagai jamur antagonis pada beberapa jamur patogen termasuk jamur Ganoderma boninense yang menyebabkan penyakit busuk pangkal batang tanaman kelapa sawit. Namun, pada pengujian di lapangan, efikasi mikroba antagonis masih menunjukkan hasil yang tidak stabil. Oleh karenaya perlu pengemasan mikroba antagonis dalam bentuk formulasi. Penelitian ini bertujuan untuk mengetahui pengaruh waktu penyimpanan formulasi biofungisida tepung terhadap viabilitas dan daya antagonis T. Pseudokoningii Rifai dalam menghambat jamur G. boninense Pat. secara in vitro. Penelitian disusun dengan menggunakan rancangan acak lengkap dengan empat perlakuan: ampas tebu (F1), sekam padi (F2), dregs (F4) dan kombinasi ampas tebu, sekam padi dan dregs (F12), dengan tiga ulangan. Hasil penelitian menunjukkan bahwa formulasi biofungisida tepung F2 (sekam padi) mampu menjaga viabilitas jamur T. pseudokoningii yang ditunjukkan dengan banyaknya jumlah konidia dan tingginya kecepatan pertumbuhan dibandingkan dengan formulasi lainnya. Jamur T. pseudokoningii pada formulasi F2 juga menunjukkan daya hambat tertinggi dibandingkan dengan formulasi lainnya setelah penyimpanan 1 dan 2 bulan. Kata kunci: biofungisida, formulasi, Ganoderma boninense, Trichoderma pseudokoningii
Pemberdayaan ibu PKK dalam pembuatan biopori berbasis limbah rumah tangga Isna Rahma Dini; Fauza Azmimelwa; Joko Setiawan; Rachmad Saputra
Unri Conference Series: Community Engagement Vol 2 (2020): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.2.24-30

Abstract

Various kinds of activities carried out by PKK mothers in filling their time working at home during the Covid-19 pandemic, one of which is farming around the yard. This farming activity certainly requires useful fertilizers to increase plant fertility. Biopores is an effort that can be made to improve soil health and trigger the natural decomposition of organic matter. The organic material used can come from kitchen waste. So far, biopores are known as an effort to overcome standing water and are still not widely used to increase soil fertility. This service aims to provide information on the manufacture and application of biopores in plants so that they can increase soil fertility in cultivated plants. The method used consists of lecturing methods, hands-on practice, and evaluating the achievement of activities using achievement measures. Based on the results of the posttest evaluation, there was an increase in the knowledge of PKK mothers about biopores, where initially only 40% increased to 100%. In addition, the direct practice of making and applying biopores carried out on one of the PKK mothers is an indicator of the high interest of PKK mothers in applying biopores. It can be concluded that community service activities are quite effective in increasing the ability and interest of PKK mothers in applying biopores to plants around their house yards.
Morphological characterization of Trichoderma spp. isolated from the oil palm rhizosphere in peat soils and its potential as a biological control for Ganoderma sp. in vitro Rachmad Saputra; Fifi Puspita; Anthony Hamzah; Irfandri; Eka Suryani
Jurnal Ilmiah Pertanian Vol. 19 No. 2 (2022): Jurnal Ilmiah Pertanian
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/jip.v19i2.9405

Abstract

Trichoderma sp. is a saprophytic fungus found in various environments, one of which is in the rhizosphere of oil palm plants which can be used to control Ganoderma and increase the resistance of oil palm plants from stem rot disease. This study aimed to characterize the morphology of Trichoderma spp. origin of peat land in oil palm plantations in Kampar Regency and screening the potential in controlling Ganoderma sp. Trichoderma spp. from oil palm rhizosphere was collected from the smallholder oil palm plantations in Deli Makmur Village, Kampar, Indonesia. This research was conducted by several observations, such as the hypovirulence test; identification of the morphology of Trichoderma spp. fungus; growth and diameter test of Trichoderma spp. fungus; test of the inhibitory ability of Trichoderma spp. fungus against Ganoderma sp. LPTUNRI-Gan002 isolate; test for hyperparasitism of the fungus Trichoderma spp., which has high antagonistic power against Ganoderma sp. isolate. Six Trichoderma spp. isolates had morphological characteristics similar to two species, i.e., Trichoderma harzianum (LPTUNRI-Trc001, Trc004, Trc005, and Trc006 isolates) and Trichoderma asperellum (LPTUNRI-Trc002 and Trc003). LPTUNRI-Trc003 had the highest diameter (90 mm), growth rate (32.66 mm/day), and the highest ability to suppress Ganoderma sp. LPTUNRI-Gan002 (91.03%) compared to the other five isolates.
Biological control agents potential and molecular identification of endophytic Trichoderma isolates originated from oil palm tissue against Ganoderma boninense Pat. Fifi Puspita; Titania T. Nugroho; Rachmad Saputra
Jurnal Ilmiah Pertanian Vol. 19 No. 2 (2022): Jurnal Ilmiah Pertanian
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/jip.v19i2.9402

Abstract

This research aimed to determine the potential of endophytic Trichoderma sp originated from oil palm plant tissue as a biological control agent and its molecular identification. The potency assay was carried out using a completely randomized design consisting of three isolates of Trichoderma sp. from different plant tissues (TR01 from root tissue, TS01 from stem tissue, and TM01 from midrib tissue) with inhibitory ability parameters. The inhibition of the growth of G. boninense was performed at the concentration of 15% and 20%, and the molecular identification of Trichoderma sp. endophyte isolates was analyzed using BLAST software. The results showed that Trichoderma sp. TM01 isolate had larger inhibitory than the other isolates on days 3 and 4 by 60% and 68%. TM01 isolate also showed inhibition to the growth of the G. boninense at 15% and 20%, with the percentage of inhibition reaching 71.79% and 82.05%. Based on phylogenetic analysis, the three endophytic isolates of Trichoderma sp. were closely related to Trichoderma virens.
Co-Authors Anthony Hamzah Desita Salbiah Eka Suryani Fauza Azmimelwa Fifi Puspita Hapsoh Hapsoh Irfandri Irfandri Isna Dini Isna Rahma Dini Joko Setiawan Muhammad Ali Sri Yoseva Titania Tjandrawati Nugroho Yetti Elfina