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Fifi Puspita
Department Of Agrotechnology, Faculty Of Agriculture, Universitas Riau, Pekanbaru, Indonesia
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Kompatibilitas dan Daya Hambat Konsorsium Trichoderma spp. Endofit terhadap Penyakit Busuk Buah Kakao Phytophthora palmivora Fifi Puspita; Muhammad Ali; Supriyadi Supriyadi
Agrikultura Vol 31, No 2 (2020): Agustus, 2020
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v31i2.26063

Abstract

Phytophthora palmivora dapat menyebabkan penyakit busuk buah kakao yang menimbulkan kerugian secara ekonomi. Pengendalian yang dapat digunakan adalah penggunaan agens hayati Trichoderma. Penggunaan Trichoderma dalam pengendalian dapat dilakukan secara tunggal maupun secara konsorsium dengan berbagai jenis Trichoderma maupun mikroba lainnya. Penelitian ini bertujuan untuk mengetahui kompatibilitas konsorsium Trichoderma spp. endofit dan memperoleh kombinasi isolat Trichoderma spp. endofit yang mempunyai daya hambat tertinggi terhadap pertumbuhan P. palmivora secara in-vitro dan terhadap penyakit busuk buah kakao. Penelitian ini dilaksanakan di Laboratorium Penyakit Tumbuhan Fakultas Pertanian Universitas Riau pada bulan Agustus hingga Oktober 2018. Penelitian dilaksanakan secara eksperimen dengan perlakuan T1= Trichoderma spp. endofit asal akar kelapa sawit (TR), T2= Trichoderma spp. endofit asal batang kelapa sawit (TS), T3= Trichoderma spp. endofit asal pelepah kelapa sawit (TP), T4= TR + TS, T5= TR + TP, T6= TS + TR, T7= TR + TS + TP, yang terdiri dari 3 tahap yaitu: 1) uji kompatibilitas jamur Trichoderma spp. endofit, 2) uji daya hambat konsorsium Trichoderma spp. endofit dan 3) uji kemampuan konsorsium Trichoderma spp. endofit untuk mengendalikan penyakit busuk pada buah kakao. Hasil penelitian menunjukkan bahwa Trichoderma endofit isolat TR-01, TS-02 dan TM-01 bersifat kompatibel sehingga dapat digabungkan. Kombinasi (TR-01+TS-02+TM-01) memiliki daya hambat yang tertinggi terhadap pertumbuhan P. palmivora secara in-vitro yaitu  72,97%. Kombinasi isolat Trichoderma tersebut juga memiliki kemampuan terbaik untuk mengendalikan penyakit busuk pada buah kakao.
UJI KEMAMPUAN EKSTRAK DAUN BEBERAPA JENIS SIRIH (Piper sp.) UNTUK MENGENDALIKAN JAMUR Aspergillus sp. PADA BENIH KACANG TANAH SECARA IN VITRO Nela Zahara; Muhammad Ali; Fifi Puspita
Konservasi Hayati Vol 16, No 1 (2020): APRIL
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/hayati.v16i1.11565

Abstract

Fungisida nabati adalah fungisida yang terbuat dari bahan-bahan alami yang banyak tersedia dia alam. Salah satu tumbuhan yang dapat dimanfaatkan sebagai fungisida nabati antara lain yaitu beberapa spesies dari tanaman sirih seperti sirih hutan (Piper aduncum L.), sirih merah (Piper crocatum L.) dan sirih hijau (Piper betle L.). Penggunaan ekstrak beberapa jenis sirih ini dapat dijadikan alternatif pengendalian yang efektif dan efisien. Penelitian ini bertujuan untuk mendapatkan jenis ekstrak sirih yang berpotensi sebagai fungisida nabati yang mampu mengendalikan jamur Aspergillus sp. Tahapan penelitian ini terdiri atas isolasi Aspergillus sp., ekstraksi dan uji penghambatan ekstrak beberapa jenis sirih terhadap jamur Aspergillus sp., uji perlakuan benih dengan ekstrak beberapa jenis sirih. Berdasarkan hasil penelitian pemberian ekstrak daun sirih hijau, sirih hutan dan sirih merah dapat menghambat pertumbuhan jamur Aspergillus sp. Dengan persentase penghambatan sebesar 32,08%, 37,53% dan 33,03% secara berurutan. Pemberian ekstrak daun sirih hijau, sirih hutan dan sirih merah juga mampu menurunkan persentase infeksi serangan jamur Aspergillus sp. sebesar 19,60%, 19,20% dan 18,80% jika dibandingkan dengan kontrol tanpa pemberian ekstrak daun sirih. Pemberian ekstrak daun beberapa jenis sirih dan tanpa ekstrak daun sirih sama-sama menghasilkan persentase kecambah normal yang tinggi (>80%).
UJI AKTIVITAS ENZIM PROTEASE DARI ISOLAT Bacillus sp. ENDOFIT TANAMAN KELAPA SAWIT (Elaeis quinensis) Atika Marnolia; Yuli Haryani; Fifi Puspita
Sistem Informasi Vol 6 No 02 (2016): Jurnal Photon
Publisher : LPPM Universitas Muhammadiyah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jp.v6i02.429

Abstract

Proteolytic bacteria are able to hydrolyze proteins into amino acid and smaller peptides. The objective of this study to determine the abilty of three Bacillus sp. isolated from leaf (BD), root (BA), and stem (BP) of palm oil to produce protease. The proteolytic activity was determine based on the amount of tyrosin liberated in Unit/mL, while the specific activity was indicated by activity per unit weight of proteins. Protein concentration of enzymes extracts was determined by Lowry method. The results showed that all isolates were able to produce protease. Protease activity of Bacillus sp. BD, BA and BP was (711,260 ± 0,735) x 10-3 U/mL, (708,536 ± 0,918) x 10-3 U/mL and (710,027 ± 0,091) x 10-3 U/mL in optimal production times of 24 hours, repectively.
RESPON BAWANG MERAH (Allium ascalonicum. L) TERHADAP PEMBERIAN TRICHOKOMPOS TKKS TERFORMULASI DAN PUPUK NITROGEN PADA LAHAN GAMBUT Azrida Syamsi; - Nelvia; Fifi Puspita
Sistem Informasi Vol 6 No 01 (2015): Jurnal Photon
Publisher : LPPM Universitas Muhammadiyah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jp.v6i01.440

Abstract

Penelitian bertujuan untuk mempelajari interaksi pemberian Trichokompos TKKS terformulasi dan pupuk nitrogen, pengaruh utama keduanya terhadap pertumbuhan dan produksi bawang merah (Allium ascalonicum. L) pada lahan gambut serta serapan hara N. Penelitian dilakukan secara ekperimen dalam bentuk faktorial 4 x 3 disusun menggunakan Rancangan Acak Lengkap (RAL). Faktor pertama yaitu Trichokompos TKKS terformulasi yang terdiri dari 4 taraf (15, 20, 25, dan 30 ton/ha) dan faktor ke dua yaitu pupuk nitrogen yang terdiri dari 3 taraf (150, 250, dan 350 kg/ha). Parameter yang diamati adalah tinggi tanaman, jumlah anakan dan bobot segar umbi per rumpun sampel. Hasil penelitian menunjukkan bahwa interaksi Trichokompos TKKS terformulasi 30 ton/ha dan pupuk nitrogen 350 kg N/ha meningkatkan bobot segar umbi per rumpun sampel secara nyata. Peningkatan dosis Trichokompos TKKS terformulasi dari 15 ke 30 ton/ha meningkatkan tinggi tanaman, jumlah anakan dan bobot segar umbi per rumpun sampel. Peningkatan dosis pupuk nitrogen dari 150 ke 350 kg N/ha meningkatkan tinggi tanaman dan bobot segar umbi per rumpun sampel.
PEMBERIAN TRICHOKOMPOS TANDAN KOSONG KELAPA SAWIT TERFORMULASI TERHADAP PERTUMBUHAN DAN HASIL TIGA VARIETAS BAWANG MERAH (Allium ascalonicum L.) DI LAHAN GAMBUT Dewi Marlina Susanti; - Herman; Fifi Puspita
Sistem Informasi Vol 7 No 02 (2017): Jurnal Photon
Publisher : LPPM Universitas Muhammadiyah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jp.v7i02.497

Abstract

This study aimed to get to know the effect of different doses formulated Trichokompos doses on the growth and production of onion varieties in peat area, as well as to get a dose of Trichokompos formulated and the best interaction on several onion varieties. Research using split plot design draft with 12 combination and each treatment was repeated 3 times, analyzed in the laboratory and analysis of variance. The main plot treatments consisted of: (V1): Varieties Bauji, (V2): Varieties Maja Cipanas, (V3): Varieties Bima Brebes. Treatment subplot, consists of 4 levels, namely: T1: (5 ton / ha), T2 (10 tons / ha), T3 (15 ton / ha) and T4 (20 ton / ha). The data obtained were statistically analyzed using analysis of variance (ANOVA) followed by HSD test at 5% level. The results of the research showed that, the provision of oil palm empty bunches Trichokompos formulated doses of 10 ton / ha and a dose of 20 ton / ha on the varieties Bauji can increase the content of chlorophyll by 78.64% and K nutrient uptake value of 2.46 mg / plant. Provision of Oil Palm Empty Fruit Bunch Trichokompos formulated doses of 10 ton / ha on Maja Cipanas varieties could increase plant height of 24.93 cm and a dose of 5 tonnes / ha can increase the diameter of the bulbs by 24.70% and the provision of oil palm empty bunches Trichokompos formulated doses of 5, 10 , 15 and 20 ton / ha on Bima Brebes varieties can increase the number of tillers, the number of bulbs, tubers fresh weight, fresh weight of the plants, the rate of photosynthesis, transpiration rate, stomata conductivity, CO2 concentration, the value of N uptake and nutrient uptake value P. Increasing doses of oil palm empty bunches Trichokompos formulated from 5 to 20 ton / ha on the varieties Bauji, Maja Cipanas and Bima Brebes not affect all parameters observed.
Morphological characterization of Trichoderma spp. isolated from the oil palm rhizosphere in peat soils and its potential as a biological control for Ganoderma sp. in vitro Rachmad Saputra; Fifi Puspita; Anthony Hamzah; Irfandri; Eka Suryani
Jurnal Ilmiah Pertanian Vol. 19 No. 2 (2022): Jurnal Ilmiah Pertanian
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/jip.v19i2.9405

Abstract

Trichoderma sp. is a saprophytic fungus found in various environments, one of which is in the rhizosphere of oil palm plants which can be used to control Ganoderma and increase the resistance of oil palm plants from stem rot disease. This study aimed to characterize the morphology of Trichoderma spp. origin of peat land in oil palm plantations in Kampar Regency and screening the potential in controlling Ganoderma sp. Trichoderma spp. from oil palm rhizosphere was collected from the smallholder oil palm plantations in Deli Makmur Village, Kampar, Indonesia. This research was conducted by several observations, such as the hypovirulence test; identification of the morphology of Trichoderma spp. fungus; growth and diameter test of Trichoderma spp. fungus; test of the inhibitory ability of Trichoderma spp. fungus against Ganoderma sp. LPTUNRI-Gan002 isolate; test for hyperparasitism of the fungus Trichoderma spp., which has high antagonistic power against Ganoderma sp. isolate. Six Trichoderma spp. isolates had morphological characteristics similar to two species, i.e., Trichoderma harzianum (LPTUNRI-Trc001, Trc004, Trc005, and Trc006 isolates) and Trichoderma asperellum (LPTUNRI-Trc002 and Trc003). LPTUNRI-Trc003 had the highest diameter (90 mm), growth rate (32.66 mm/day), and the highest ability to suppress Ganoderma sp. LPTUNRI-Gan002 (91.03%) compared to the other five isolates.
Biological control agents potential and molecular identification of endophytic Trichoderma isolates originated from oil palm tissue against Ganoderma boninense Pat. Fifi Puspita; Titania T. Nugroho; Rachmad Saputra
Jurnal Ilmiah Pertanian Vol. 19 No. 2 (2022): Jurnal Ilmiah Pertanian
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/jip.v19i2.9402

Abstract

This research aimed to determine the potential of endophytic Trichoderma sp originated from oil palm plant tissue as a biological control agent and its molecular identification. The potency assay was carried out using a completely randomized design consisting of three isolates of Trichoderma sp. from different plant tissues (TR01 from root tissue, TS01 from stem tissue, and TM01 from midrib tissue) with inhibitory ability parameters. The inhibition of the growth of G. boninense was performed at the concentration of 15% and 20%, and the molecular identification of Trichoderma sp. endophyte isolates was analyzed using BLAST software. The results showed that Trichoderma sp. TM01 isolate had larger inhibitory than the other isolates on days 3 and 4 by 60% and 68%. TM01 isolate also showed inhibition to the growth of the G. boninense at 15% and 20%, with the percentage of inhibition reaching 71.79% and 82.05%. Based on phylogenetic analysis, the three endophytic isolates of Trichoderma sp. were closely related to Trichoderma virens.
UJI BEBERAPA ISOLAT JAMUR ENDOFIT TERHADAP JAMUR TULAR BENIH DAN PERTUMBUHAN BIBIT CABAI MERAH Nurhafida Nurhafida; Fifi Puspita
Jurnal Agroteknologi Vol 13, No 1 (2022): Agustus 2022
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/ja.v13i1.12097

Abstract

The aim of this study was to test several endophytic fungi isolates with high antagonistic power against seed borne fungi in vitro and their effect on the growth of chili (Capsicum annuum L.) seedlings in vivo. This research was conducted at the Plant Disease Laboratory and Greenhouse Faculty of Agriculture, Riau University from October 2018 to February 2019. This research was conducted experimentally using a completely randomized design (CRD) consisting of 5 treatments and 4 replications. The treatment of endophytic fungi was applied using the seed immersion method. The results showed that giving the endophytic fungi Rhizictonia sp., Cephaloporium sp., T. virens from oil palm roots and T. virens from oil palm stems were able to suppress the incidence of seed-borne pathogenic fungi Fusarium sp., Colletotrichum sp. and Aspergillus sp.. Provision of endophytic fungus T. virens from oil palm roots can increase seed germination capacity by 14%, number of live seeds by 17% and increase seedling height and leaf number.
UJI ANTAGONISME ISOLAT JAMUR ENDOFIT TANAMAN PINANG TERHADAP GANODERMA BONINENSE PAT. PENYEBAB PENYAKIT BUSUK PANGKAL BATANG KELAPA SAWIT Muhammad Ali; Fitra Ananda Amimartha; Fifi Puspita
Jurnal AGROHITA: Jurnal Agroteknologi Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan Vol 8, No 1 (2023): JURNAL AGROHITA
Publisher : Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jap.v8i1.7982

Abstract

Kelapa sawit merupakan salah satu komoditas perkebunan utama di Provinsi Riau yang memberikan kontribusi devisa yang tinggi. Penelitian ini bertujuan untuk menyeleksi dan mengisolasi fungi endofit tanaman pinang yang memiliki potensi antagonis tertinggi sebagai agens hayati terhadap G. boninense yang menyebabkan penyakit busuk pangkal batang kelapa sawit dan mengidentifikasi hingga tingkat genus. Penelitian dilakukan di Laboratorium Penyakit Tumbuhan, Fakultas Pertanian, Universitas Riau, Pekanbaru. Penelitian yang digunakan metode eksplorasi, observasi dan eksperimen. Penelitian terdiri dari 6 langkah: isolasi dan pemurnian jamur endofit tanaman pinang, uji hipovirulen jamur endofit tanaman pinang, uji diameter dan kecepatan tumbuh jamur endofit tanaman pinang, uji antagonis jamur endofit tanaman pinang terhadap G.boninense, uji hiperparasitisme jamur endofit tanaman pinang yang memiliki daya antagonis tinggi terhadap G.boninense dan identifikasi isolat jamur endofit. Hasil dari penelitian ditemukan 17 isolat jamur endofit, 10 isolat bersifat hipovirulen dan 7 isolat bersifat virulen. Isolat P2 memiliki nilai daya antoginis tertinggi yaitu sebesar 77,67% dan diameter serta kecepatan pertumbuhan isolat P2 (90 mm dan 33,45 mm/hari). Tipe Hiperparasit isolat P2 adalah pelilitan, isolat P9 adalah lisis dan penjeratan (isolat P16). Hasil penelitian menunjukkan bahwa isolat P2 termasuk dalam genus Trichoderma dan isolat P9 termasuk dalam genus Cylindrocladium.
EKSPLORASI JAMUR ENDOFIT DARI TANAMAN PADI GOGO (Oryza sativa L.) DAN UJI DAYA ANTAGONISNYA TERHADAP JAMUR Pyricularia oryzae PENYEBAB PENYAKIT BLAS Muhammad Ali; Fifi Puspita; Rezki A. Nugroho
Jurnal AGROHITA: Jurnal Agroteknologi Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan Vol 8, No 3 (2023): JURNAL AGROHITA
Publisher : Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jap.v8i3.8975

Abstract

Tujuan dari penelitian ini adalah untuk mendapatkan isolat jamur endofit dari tanaman padi gogo dengan kemampuan antagonis terbaik dalam menghambat pertumbuhan jamur P. oryzae. Penelitian ini terdiri dari 7 tahapan meliputi tahap pengambilan sampel, isolasi dan purifikasi jamur endofit dan P. oryzae, uji hipovirulensi jamur endofit; uji antagonis jamur endofit terhadap jamur P. oryzae; uji pertumbuhan koloni, uji hiperparasitisme, dan karakterisasi jamur endofit berdaya antagonis tinggi. Uji antagonis dilakukan dengan menggunakan Rancangan Acak Lengkap yang terdiri dari isolat endofit yang berasal dari jaringan batang, pelepah dan daun padi. Data yang diperoleh dianalisis deskriptif dan sidik ragam variance followed by Ducan's new multiple range test (DNMRT) pada taraf 5%. Hasil eksplorasi, didapatkan 11 isolat jamur endofit dari tanaman padi gogo dengan 9 isolat bersifat non-patogenik. Hasil uji antagonis jamur endofit diperoleh 7 isolat berdaya antagonis tinggi. isolat PG5 memiliki daya antagonis tertinggi sebesar 60%  diikuti PG2 (56%), PG3 (56%), PG4 (54%), PG7 (54%), PG6 (53%), dan PG11 (52%). Berdasarkan hasil karakterisasi, isolat PG2, PG6 dan PG7 mirip dengan Trichoderma sp., Isolat PG3 dan PG5mirip dengan Aspergillus sp., isolat PG4 mirip dengan Penicillium sp., and isolat PG11mirip dengan Nigrospora sp.
Co-Authors - Herman Adja Muhammad Alsan Shaf Anthony Hamzah Arif Fashli Hakiki Arman Effendi AR Arya Arismaya Metananda Atika Marnolia Azrida Syamsi Defri Yoza Dewi Marlina Susanti Eka Suryani Erlida Ariani Evi Sribudiani Fetmi Silvina Fitra Ananda Amimartha Hafiz Fauzana Idwar, , Irfandri Irfandri Lestari Magdalena Muhammad Ali Muhammad Ali Nela Zahara Nelvia Nelvia Nurhafida Nurhafida Pajri Ananta Yudha Pebriandi, Pebriandi Rezki A. Nugroho Riska Pebrianti Rizky Ramadhoni RUSLI RUSTAM Saputra, Rachmad Somadona, Sonia Supriyadi Supriyadi Susilawati Susilawati Titania Tjandrawati Nugroho Yuli Haryani