Garuda - Garba Rujukan Digital

Microbial Isolates in Microplastic-Polluted Soil Battah, Moses Victor; Olawale, Otitoju; Abah, Moses; John, Umaru Isaac; Dauda, Maianguwa Abdulrashid; Joel, Emmanuel Askeb-nde; Vera, Caleb; Umaru, Kerenhappuch Isaac
African Journal of Biochemistry and Molecular Biology Research Vol 1 No 1 (2024): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v1i1.3705

Microplastic pollution is a growing environmental concern, with plastic debris fragmenting into microscopic particles that contaminate soil ecosystems. Microplastics have become a major environmental concern due to their persistent presence in various ecosystems, including soil. They can act as pollutants and have detrimental effects on the environment and human health. This study aimed at isolating and identifying microbes (Bacteria and Fungi species) present in microplastic-polluted soil. Microplastic-polluted soil samples were collected from three sites (which were Kwararafa University Area Wukari to serve as the site 1, Federal Road Safety Commission Office Area Wukari to be the site 2, while Marmara Area Wukari to be the site 3), from two points; A and B and then packaged in brown envelopes which were then transferred to the Laboratory for further analysis after air-drying them for 24 hours. Then 1gram of each soil sample was weighed and suspended in 9mls of distilled water respectively; Six (6) folds of distilled water were prepared for serial dilution of each soil sample. 1ml of each sample was pour plated on NA, MAC, BA, EMB, CLED and SDA respectively. Then a smear of bacteria was prepared and allow to air dry for 30min and heat fix using flame after which was viewed under the microscope. The results obtained from this study showed that Staphylococcus spp are large, medium, small, and pinpoint in size, irregular and spherical in shape, light pink, creamy grey in color, smooth, mucoid and dried texture. Escherichia coli and Klebsiella spp here has similar morphological characteristics, both in size, shape, color, texture, margin and elevation as Staphylococcus spp. Bacillus spp and Pseudomonas spp here has similar morphological identification but differ only in their shape where Bacillus spp has a filamentous shape but Pseudomonas spp has rhizoid shape respectively. In terms of texture, Bacillus spp are Smooth, dried and mucoid but Pseudomonas spp are only smooth, dried texture. Bacillus spp and Pseudomonas spp only differ in their cell morphology where Bacillus spp appears to be rods with spores while Pseudomonas spp appears as short and long rods in pairs. They react positive to catalase, oxidase and citrate tests while negative to indole test. Aspergillus Niger and Rhizopus stolonifer were found to be the major fungi isolated from the collected sample. Aspergillus Niger and Rhizopus stolonifer are spherical in shape and are large in size. Aspergillus Niger appears powdery and Rhizopus stolonifer appears to fluffy, wood-like. In terms of color and pigmentation Aspergillus Niger species are black, black creamy on reverse while Rhizopus stolonifer appears grayish in color. The discovery of these microbes in microplastics-polluted soil offers a ray of hope for mitigating plastic pollution. By delving deeper into their capabilities and fostering collaboration between microbiologists and environment. This research will contribute to a broader understanding of the ecological impact of microplastics on soil health and functioning. By identifying microbes associated with microplastics, the specific mechanism of action employed by these bacteria in degrading microplastics can be studied subsequently.

Anti-Inflammatory and Anti-Microbial Effects of Prosopis Africana Methanol Stem Back Extract Aduwamai, Umaru Hauwa; Oluwatosin, Jegede Olosola; Malgwi, Jemimah Mohammed; Umaru, Kerenhappuch Isaac
African Journal of Biochemistry and Molecular Biology Research Vol 1 No 1 (2024): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v1i1.3732

The anti-inflammatory and anti-microbial effect of Prosopis Africana methanol stem bark extract was determined. Results of qualitative phytochemical screening revealed the presence of tannins, saponins, flavonoids, alkaloids, phenols, steroids and terpenoids. The quantitative analysis revealed the quantity of alkaloids (0.11%), tannins (1.92%), phenols (3.77%), flavonoids (0.77%), steroids (0.14%), terpenoids (0.21%) and saponins (4.01%). The anti-inflammatory effect of methanol stem-bark extract of Prosopis africana was assessed in male albino rats using paw immersion method. The anti-inflammatory effect produced by methanol stem-bark extract of Prosopis africana was found to be significant (p<0.05) at the dose of 200mg/kg, which was more effective than other concentrations at time interval of 0-90minutes. The antimicrobial activities of the methanol stem-bark extract of Prosopis africana produced the highest zone of inhibition (l7mm) on Pseudomonas aeruginosa and the lowest zone of inhibition (11mm) on Staphylococcus aureus. The minimum inhibitory concentration value of Prosopis Africana methanol stem-bark extract against E.coli, S. aureus and P. areuginosa was 2.5mg/ml each compared to K. pneumonia which was 5.5mg/ml. Results obtained indicate the antimicrobial effect of the methanol stem-bark extract of Prosopis Africana against K. pneumoniae was more effective than ampicillin. This work has further supported the basis for the use of the plant as anti-inflammatory agent in the treatment of various diseases associated with the microorganisms studied.

Effect of Administration of Copper plasma Activated water and Anesthetic on Hematological and Pancreatic tissues of Wister rats Umaru, Isaac John; Kingsly, Iseko Iyoko; Gyasi, Prince Kwadwo; Ogochukwu, Edom Favour; Akafa, Tensaba Andes; Malantso, Jackson Soyinka; Habibu, Bilyaminu; Umaru, Kerenhappuch Isaac
African Journal of Biochemistry and Molecular Biology Research Vol 2 No 1 (2025): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v2i1.4722

In biomedicine, applications of PAW span from biofilm removal, wound healing, deactivation of bacteria and viruses, dentistry (for teeth disinfection and whitening), and cancer therapy. The biochemical activity of PAW is derived from synergistic effects of the highly reactive species, specifically reactive oxygen and nitrogen species (RONS). PAW is considered a biofriendly and prospective solution for biotechnology applications due to the time dependent nature of its biochemical activity because of the active species, and its economic and environmental benefits of using air rather than toxic chemicals as the raw material. Thus, the study on Effect of Administration of Copper plasma Activated water and Anaesthetic on Haematological and Pancreatic tissues of Wister rats. Material and Methods: Preparation of plasma-activated water a non-thermal micro-hollow cathode discharge (MHCD) was used to generate plasma-activated waters (CU-PAWs). Results: The results showed the haematological profile of the animals treated with 400mgkg-' had a decrease in Haemoglobin, Mean capsular Haemoglobin (11.21 ± 1.23 and 22.93 ± 21.11) compared to the controlled group (15.03 ± 1.72 and 29.93 ± 7.27) and in animals treated with 200mgkg-1 and 400mgkg-1 there is a significant decrease in the platelets (21.96 ± 23.16 and 20.89 ± 24.03) compared to animals on control group (32.08± 16.70) (table 2). Animals treated with 200mgkg-' and 400mgkg-' have a decrease in platelet (22.36 ± 4.88 and 20.89 ± 24.03) compared to animals on control (32.08 ± 16.70) and also animals treated with 400mgkg-1 have an elevated white blood cell count 173-56 ± 4.50) compared to the control group (3.66 ± 3.06). Conclusion: Cupper plasma water is safe to be used since it do not have much effect on the blood biochemistry and haematological parameters unlike the aesthetic agents cause changes on the haematological and blood biochemistry parameters. Hence, it is important to be aware of the effects of these agents before using them in experiments.

Zinc Oxide Based Nanomaterials and Their Application in the Removal of Toxic Contaminants for Waste Water Matilda, Manasseh Ilumunter; Umaru, Isaac John; Umaru, Kerenhappuch Isaac; Akram, Muhammad
African Journal of Biochemistry and Molecular Biology Research Vol 2 No 2 (2025): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v2i2.5893

The increasing global demand for clean water underscores the urgency of addressing environmental pollution, particularly through effective wastewater treatment. Rapid urbanization, population growth, and industrial expansion have significantly contributed to the degradation of water quality, making contamination a persistent and complex challenge. Despite numerous scientific efforts to develop remediation strategies, many existing techniques remain difficult to replicate or scale down effectively for widespread application. In this context, nanotechnology has emerged as a promising field, offering innovative solutions for environmental remediation. Among various nanomaterials, zinc oxide (ZnO) nanoparticles stand out due to their unique physicochemical and optical properties, which are substantially enhanced at the nanoscale. ZnO nanoparticles have gained considerable attention in material science, especially for their applications in photocatalysis, antimicrobial treatments, and environmental detoxification. However, their synthesis and functional performance are highly sensitive to several factors, including the pH of the reaction medium, calcination temperature, reaction time, stirring speed, the nature of capping agents, and the concentration of metal precursors. These variables critically influence the size, morphology, and surface activity of ZnO nanoparticles, thereby determining their effectiveness in water treatment applications. Understanding and optimizing these synthesis parameters are crucial for enhancing the reproducibility, efficiency, and scalability of ZnO-based nanotechnologies in real-world environmental contexts.

Hypoglycaemic Potential of Methanol Stem-Bark Extract of Mangifera Indica in Alloxan Induced Diabetic Albino Rats and Its Toxicity Effect to Liver and Kidney Umaru, Isaac John; Ekubnse, Nkiri Annasthesia; Umaru, Kerenhappuch Isaac; Tansaba, Akafa Andes; Ugoeze, Egeonu Stephen; Nanmar, Chakfa
African Journal of Sciences and Traditional Medicine Vol 1 No 1 (2024): African Journal of Sciences and Traditional Medicine
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajstm.v1i1.3358

Mangifera indica (MI), popularly known as mango belong to the genus Mangifera and family Anacardiacea. The leaves, stem bark and roots are used as herbal medicines worldwide. Mango possesses anti-diabetic, anti-bacterial, anti- oxidant, anti-viral and anti-inflammatory properties. This research focuses on the hyperglycemic and antibacterial capacity of methanol stem bark extract of Mango tree in alloxan induced diabetic albino rats to combat its fatal consequences in humans. This work is carried out to determine the hypoglycemic capacity of methanol stem bark extract of Mangifera indica in alloxan-induced diabetic albino rats at different volumes (ml) of administration, to also determine the antibacterial capacity of methanol stem bark extract of Mangifera indica in alloxan-induced diabetic albino rats at different volumes (ml) of administration and to test the efficacy of methanol stem bark extract of Mangifera indica in alloxan-induced diabetic albino rats. Fresh stem bark (trunk) of Mangifera indica were collected in the Federal University Wukari school premises, were air dried for 21 days, pounded into powder, cold macerated in methanol, filtered and extract was gotten. 27 diseased-free (healthy) albino rats which were purchased at Yola, Adamawa State, were kept in different cages to enable proper analysis at the cause of the work. Results of liver function indices showed that treatments with 100 mg/kg, 200mg/kg and 400mg/kg body weight of Mangifera indica stem bark methanolic extract were able to restore liver function parameters at different levels of concentrations. Treatments with standard drug and all the doses of the extract were able to counteract the elevated effect of alloxan in some kidney parameters such as serum urea level at 200mg/kg with (25.23±1.98a) and (1.05 ±0.14a) for creatinine at 200mg/kg respectively. The result of this study shows that the stem back extract possessed as an agent for hypoglycaemic. These can serve as possible source of raw material for pharmaceutical products. However, the extract is found not to be harmful to the liver and kidney and should be used as health remedy for certain diseases such as hyperglycaemic and bacterial effects.

Antibacterial and Antifungal Potential of Methanol Stem–Bark Extract of Mangifera Indica Umaru, Kerenhappuch Isaac; Tansaba, Akafa Andes; Ugoeze, Egeonu Stephen; Nanmar, Chakfa; Umaru, Isaac John
African Journal of Sciences and Traditional Medicine Vol 1 No 1 (2024): African Journal of Sciences and Traditional Medicine
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajstm.v1i1.3360

Medicinal plants have served through the ages, as a constant source of medicaments for the exposure of variety of diseases. The history of herbal medicine is as old as human civilization. The plants are known to provide a rich source of botanical anthelmintic, antibacterial and insecticides. Mangifera indica (MI), popularly known as mango belong to the genus Mangifera and family Anacardiacea. The leaves, stem bark and roots are used as herbal medicines worldwide. In antibacterial analysis, higher growth inhibition rate was observed at 400 ug/ml for Klebsiella pneumonia with 20.55 + 1.26μg/mL indicating that the extract has effect at a higher concentration than when treated at a lower concentration. In blood glucose level analysis, administration of 100 mg/kg body weight of Mangifera indica stem bark methanolic extract showed reduced blood glucose level in day 14 with 5.11 ± 0.14a of treatment when compared to normal control.

Effects of AlCl3 on the Liver Function of Wistar Rats Treated with Moringa oleifera Seed Extracts Yusufu, Dawoye; Zinas, Simon Teza; Shenia, Zephaniah Hananiah; C, Ugwuoke Kenneth; Umaru, Isaac John; Ejeh, Yakubu Ojochenemi; Grace, Onwubiko N.; Henry, Onwubiko A.; Audu, Anthony Mishara; Umaru, Kerenhappuch Isaac
African Journal of Sciences and Traditional Medicine Vol 1 No 1 (2024): African Journal of Sciences and Traditional Medicine
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajstm.v1i1.3691

In this study ALT and AST levels of experimental rats were significantly (p<0.05) increased from 11.91 ± 0.20 and 11.83 ± 0.40 to 57.23 ± 0.24 and 59.29 ± 0.50 respectively due to intoxication with aluminum chloride. When rats were treated with ethanol and aqueous extracts of Moringa oleifera at 100 mg/kg the levels of ALT dropped down to 11.62 ± 0.40 and 11.61 ± 0.17 respectively and the AST levels dropped to 11.39 ± 0.25 and 11.42 ± 0.15 respectively. A further increase in the concentrations of both extracts to 400 mg/kg resulted to a significant elevation of ALT 11.93 ± 0.17 (ethanol) and 11.94 ± 0.07 (aqueous) and AST 11.77 ± 0.25 (ethanol) and 11.78 ± 0.15 (aqueous). AST and ALT are common liver enzymes because of their higher concentrations in hepatocytes, but only ALT is remarkably specific for liver function. Therefore, an elevation in serum concentration of ALT is an indication of liver damage. The preliminary phytochemical screening for seed extracts of Moringa oleifera revealed that flavonoids, terpenoids, phenols, alkaloids, steroids and reducing sugars tannins were present in both the ethanol and aqueous extracts. Saponins and tannins were only found in the aqueous and ethanol extracts respectively. Experiments to observe for lethal conditions or changes in behavior showed no lethality or behavioral change at doses of 10, 100, 1000, 1600 and 2900 mg/kg bw. Weakness and drowsiness was exhibited at a dosage of 5000 mg/kg bw but no death occurred within 24 hrs. of administration.

Effects of Brassica oleraceae Leaves on Serum Liver Function of Wistar Rats Intoxicated with Prednisolone Amen, Ananias; Yusufu, Dawoye; Oladele, Gloria Omonefe; Umaru, Kerenhappuch Isaac
African Journal of Sciences and Traditional Medicine Vol 1 No 1 (2024): African Journal of Sciences and Traditional Medicine
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajstm.v1i1.3692

The recent study investigated the effects of ethanol extract of Brassica oleracaeae leaves on prednisolone induced toxicity in male albino rats. Liver markers were assayed in order to investigate the toxic effect of prednisolone and the ameliorating effects of the extract. Sixteen (16) rats grouped in to four (n=4) were administered prednisolone and ethanol leaves extract based on the experimental design. The male albino rats were sacrificed after the experimental period of fourteen (14) days, blood was collected for assay of the liver function by ocular puncture. Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT) levels were significantly (p<0.05) increased from 5.67 ± 5.13 and 6.67 ±7.02 to 26.67 ± 8.02 and 25.00 ± 4.00 respectively when rats where administered with prednisolone toxicity. Upon treatment of rats with ethanol leaves extract of B. oleraceae at 100mg/kg, the concentration of AST and ALT were lowered to 7.67 ± 6.81 and 8.00 ± 7.55 respectively. A further increased in the concentration of the extract to 300mg/kg resulted to a significant (p<0.05) increased of AST (9.33 ± 8.12) and ALT (12.00 ± 10.00) levels. Similarly, the levels of alkaline phosphatase (ALP) and Albumin increases in the group that were administered with prednisolone however, when rats were treated with ethanol leaves extract of Brassica oleracaeae such concentrations were decreased. The findings of this study can be concluded that, the ethanol leaves extract of Brassica oleracaeae showed capacity in ameliorating the effects induced by prednisolone intoxication.

Effects of AlCl3 on the Enzymatic Antioxidants of Wister Rats Treated with Moringa oleifera Seed Extracts Yusufu, Dawoye; Zinas, Simon Teza; Habibu, Bilyaminu; Amen, Ananias; Umaru, Isaac John; Ejeh, Yakubu Ojochenemi; Grace, Onwubiko N.; Henry, Onwubiko A.; Audu, Anthony Mishara; Umaru, Kerenhappuch Isaac
African Journal of Sciences and Traditional Medicine Vol 1 No 1 (2024): African Journal of Sciences and Traditional Medicine
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajstm.v1i1.3693

Determination of Malondialdehyde, MDA in blood plasma or tissue homogenates is one of the useful methods to predict the oxidative stress levels. The current study investigates the ameliorative effects of the seed extracts of Moringa oleifera on 35 albino rats induced with AlCl3 toxicity. Biomarkers of oxidative stress (Superoxide Dismutase, SOD; Catalase, CAT; Glutathione Peroxidase, GPx and Malondialdehyde, MDA were assayed. The plant seed extracts were shown to reduce the levels of MDA increased by AlCl3. AlCl3 caused decrease in (glutathione peroxidase) GPx levels as it causes MDA to significantly get elevated. The results showed that GPx decreased from 9.48 ± 0.86 to 6.68 ± 1.73 but upon treatments with 100 mg/kg bw of M. oleifera, GPx levels increased to 8.84 ± 0.86 (ethanol) and 8.96 ± 0.86 (aqueous). Increasing the concentrations of the extracts further increased the GPx levels while MDA were reduced.

Hypoglycaemic Potential of Methanol Stem-Bark Extract of Mangifera Indica in Alloxan Induced Diabetic Albino Rats and Its Toxicity Effect to Liver and Kidney Umaru, Isaac John; Ekubnse, Nkiri Annasthesia; Umaru, Kerenhappuch Isaac; Tansaba, Akafa Andes; Ugoeze, Egeonu Stephen; Nanmar, Chakfa
African Journal of Sciences and Traditional Medicine Vol 1 No 1 (2024): African Journal of Sciences and Traditional Medicine
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajstm.v1i1.3358

Mangifera indica (MI), popularly known as mango belong to the genus Mangifera and family Anacardiacea. The leaves, stem bark and roots are used as herbal medicines worldwide. Mango possesses anti-diabetic, anti-bacterial, anti- oxidant, anti-viral and anti-inflammatory properties. This research focuses on the hyperglycemic and antibacterial capacity of methanol stem bark extract of Mango tree in alloxan induced diabetic albino rats to combat its fatal consequences in humans. This work is carried out to determine the hypoglycemic capacity of methanol stem bark extract of Mangifera indica in alloxan-induced diabetic albino rats at different volumes (ml) of administration, to also determine the antibacterial capacity of methanol stem bark extract of Mangifera indica in alloxan-induced diabetic albino rats at different volumes (ml) of administration and to test the efficacy of methanol stem bark extract of Mangifera indica in alloxan-induced diabetic albino rats. Fresh stem bark (trunk) of Mangifera indica were collected in the Federal University Wukari school premises, were air dried for 21 days, pounded into powder, cold macerated in methanol, filtered and extract was gotten. 27 diseased-free (healthy) albino rats which were purchased at Yola, Adamawa State, were kept in different cages to enable proper analysis at the cause of the work. Results of liver function indices showed that treatments with 100 mg/kg, 200mg/kg and 400mg/kg body weight of Mangifera indica stem bark methanolic extract were able to restore liver function parameters at different levels of concentrations. Treatments with standard drug and all the doses of the extract were able to counteract the elevated effect of alloxan in some kidney parameters such as serum urea level at 200mg/kg with (25.23±1.98a) and (1.05 ±0.14a) for creatinine at 200mg/kg respectively. The result of this study shows that the stem back extract possessed as an agent for hypoglycaemic. These can serve as possible source of raw material for pharmaceutical products. However, the extract is found not to be harmful to the liver and kidney and should be used as health remedy for certain diseases such as hyperglycaemic and bacterial effects.

Title

Found 54 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 54 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 54 Documents
Search