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All Journal Jurnal Medik Veteriner Community Engagement and Emergence Journal (CEEJ) Ovozoa: Journal of Animal Reproduction Jurnal Kedokteran Hewan
Amrullah, Muhammad Fajar
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

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Genetic Analysis of The Leptin Gene in Goats Based on GenBank DNA Sequences Amrullah, Muhammad Fajar; Utomo, Budi; Utama, Suzanita; Suprayogi, Tri Wahyu; Lestari, Tita Damayanti; Restiadi, Tjuk Imam; Belgania, Ristaqul Husna
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.125-131

Abstract

The Leptin gene is the gene that produces the leptin hormone, which is released from adipose tissue and can increase the productivity of animals. This study aimed to identify polymorphic nucleotides, changes in amino acid components, and species of goats based on GenBank Leptin DNA sequence data. A total of five goat leptin DNA sequences were extracted from NCBI GenBank data. The leptin DNA sequence was aligned with Bioedit to locate SNPs and amino acid changes. The tree produces cultivars grown using Clustal Omega Ver. 1.2.4. Based on the DNA sequencing results of leptin genes in five goats, five SNPs were located in the coding sequence (CDS), SNPs g.17T/A, g.43T/A, g.74G/A, g.93C/A. and d. 386A/G. SNP was a missense mutation and a silent mutation. The analysis of phylogenetic trees of Leptin showed that there were three breeds of goats in one branch and two breeds of goats in different branches. These results provided the first report for further studies on the genetic diversity of leptin genes in different local goat breeds.
Detection of Melanocortin Receptor Type 4 (MC4R) Gene in Semen of Etawah Crossbreed and Senduro Goats Belgania, Ristaqul Husna; Utomo, Budi; Mustofa, Imam; Kholifah, Yayuk; Amrullah, Muhammad Fajar
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.209-215

Abstract

Etawah crossbreed and Senduro goats as superior local goats and biological genetic resources that must be preserved in Indonesia. Melanocortin receptor type 4 (MC4R) is the primary gene that regulates food intake and energy balance which can be used as a candidate marker for livestock selection genes. This study aimed to analyze the comparison of cement quality and detect the profile of the MC4R gene in Etawah crossbreed and Senduro goat semen. The samples of this study were Etawah crossbreed and Senduro goat semen. Macroscopic examination of cement includes the evaluation of volume, consistency, odor, color, and pH. Microscopic examination of semen includes motility, abnormalities, and spermatozoa concentration. Examination of motility was performed in the condition of fresh semen, after the addition of diluents, and post-thawing of frozen straw. The polymerase chain reaction (PCR) method was performed to amplify specific fragments of the MC4R gene. There was a decrease in spermatozoa motility before freezing and post-thawing frozen semen compared to fresh semen. The quality of frozen straw post-thawing Etawah crossbreed and Senduro goat was still worth using for artificial insemination and in vitro embryo production. The MC4R gene can be detected in liquid and frozen semen from Etawah crossbreed and Senduro goats using a PCR test in the form of a single band along 642 bp.
Reproductive disorders of cows in several villages of Kedamean district, Gresik regency, East Java, Indonesia in 2023 Utomo, Budi; Rimayanti, Rimayanti; Restiadi, Tjuk Imam; Amrullah, Muhammad Fajar
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.18-29

Abstract

Reproductive disorders are the main cause of economic losses in beef herds. This study aimed to determine the type of reproductive disorder in beef cows in Kedamean district, Gresik regency, related to feeding, age, and parity. The method used for this study was a survey. Primary data was obtained from field surveys by interviewing farmers, animal health officials, inseminators, and direct inspection of cows and pens. Secondary data was obtained from the Gresik Animal Husbandry and Animal Health Service. The data obtained was then compiled and performed descriptively. The results showed that the number of productive cows in this study area was 935, of which 340 (36.36%) had reproductive disorders. The highest type of reproductive disorder was ovarian hypofunction (OHF) (14.55%, 136/935) and the smallest was cystic ovaries (0.11%, 1/935). Cows aged two and three years (heifers and first parity) have a percentage of reproductive disorders of more than 60% compared to cows of other ages, most of them (13.82% and 12.65%) were OHF. The cows fed straw have a greater percentage of reproductive disorders than cows fed field grass. Cases of OHF engaged the first position followed by cases of silent estrus and persistent corpus luteum. It could be concluded that reproductive disorders in the form of OHF, persistent corpus luteum, and silent estrus in several villages in Kedamean district, Gresik regency, East Java, Indonesia in 2023 mostly occured at the age of two to three years on heifers or first parity, fed straw.
THE EFFECT OF BREED AND BODY CONDITION SCORE ON OVARIAN WEIGHT, NUMBER OF FOLLICLES, AND OOCYTE QUALITY IN RETIRED COWS Putri, Natalie Lovena Pramono; Ikhsan, Dilla Amalia Bilqis; Raharjo, Dadik; Khairullah, Aswin Rafif; Lestari, Tita Damayanti; Mustofa, Imam; Rimayanti, Rimayanti; Safitri, Erma; Amrullah, Muhammad Fajar; Ilmi, Laily 'Ulya Nurul; Pasang, Gabriel Sampe; Supriyadi, Supriyadi
Jurnal Kedokteran Hewan Vol 19, No 1 (2025): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v19i1.44392

Abstract

The aim of this study was to determine whether breed and body condition score (BCS) can influence ovarian weight, follicle number, and oocyte quality. This study used 90 ovaries from 15 Friesian Holstein crossbreed, 15 Ongole crossbreed, and 15 Limousin crossbreed cows obtained from an abattoir. Before the ovaries were taken, the BCS of the cows was determined. The BCS system used in this study is BCS with a scale of 1 to 5. Ovaries were weighed, follicles were measured and grouped by size (2 mm, 2-6 mm, 6 mm) then counted, oocytes were aspirated from the follicles then observed under a microscope. Oocyte quality was observed and then grouped into grade A, B, C, D based on cumulus oocyte complex (COCs). Data were analyzed using two way ANOVA and chi square test. The results of the analysis showed that breed did not influence ovarian weight, but influenced the number of follicles, and oocyte quality. In Friesian Holstein crossbreed cows, BCS affects ovarian weight, follicle number, and oocyte quality. In Ongole crossbreed cows, BCS had no effect on ovary weight, but did affect follicle number and oocyte quality. In Limousin crossbredcows, BCS had no effect on ovary weight and follicle number, but did affect oocyte quality.
Analisis Pengaruh Service Quality dan Product Quality Terhadap Customer Loyalty dengan Customer Satisfaction Sebagai Mediator pada Percetakan CV. Citra Mentari, Malang Amrullah, Muhammad Fajar; Kaihatu, Thomas Stefanus
Community Engagement and Emergence Journal (CEEJ) Vol. 6 No. 5 (2025): Community Engagement & Emergence Journal (CEEJ)
Publisher : Yayasan Riset dan Pengembangan Intelektual

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37385/ceej.v6i5.9141

Abstract

Era globalisasi saat ini, perkembangan dunia usaha mengalamai persaingan yang cukup signifikan. Banyak faktor yang memperngaruhi kemajuan dunia bisnis saat ini. Media cetak merupakan salah satu alat pemasaran yang sampai saat ini masih menjadi alternatif yang digunakan oleh berbagai perusahaan untuk menunjang strategi pemasarannya, seperti brosur, katalog, poster, flayer, dan lainnya. CV Citra Mentari Malang merupakan perusahaan yang bergerak pada percetakan offset ini berdiri pada tahun 2007 di Malang, kota Malang merupakan kota pendidikan dan banyak Lembaga pendidikan terutama perguruan tinggi seperti Universitas Brawijaya, Universitas Negeri Malang, Universitas Muhammdiah Malang, dll. CV. Citra Mentari Malang memiliki kegiatan produksi seperti pecetakan brosur, percetakan dokumen, penjilidian dokumen, fotocopy document, percetakan offset, percetakan pamflet, percetakan benner, dan lainnya. Jumlah sampel dalam penelitian ini sebanyak 96 responden yang merupakan konsumen pada pada CV. Citra Mentari Malang. Metode survei dengan pembagian kuesioner secara online melalui google form digunakan dalam penelitian ini untuk melakukan pengambilan sampel. Metode pengambilan sampel menggunakan non-probability sampling. Hasil pada penelitian ini menunjukkan bahwa terdapat pengaruh Service Quality Dan Product Quality Terhadap Customer Loyalty Dengan Customer Satisfaction Sebagai Mediator Pada Percetakan Cv. Citra Mentari, Malang.
Co-Authors Belgania, Ristaqul Husna Budi Utomo Erma Safitri Ikhsan, Dilla Amalia Bilqis Ilmi, Laily 'Ulya Nurul IMAM MUSTOFA Khairullah, Aswin Rafif Kholifah, Yayuk Pasang, Gabriel Sampe Putri, Natalie Lovena Pramono Raharjo, Dadik Rimayanti Rimayanti Supriyadi Supriyadi Suzanita Utama Thomas Stefanus Kaihatu Tita Damayanti Lestari Tjuk Imam Restiadi Tri Wahyu Suprayogi