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All Journal Jurnal Laboratorium Khatulistiwa Harmoni Sosial : Jurnal Pengabdian Dan Solidaritas Masyarakat
Ramadhani, Natasya Intan
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Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Other

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Pengaruh Konsentrasi Ekstrak Metanol Biji Buah Bungli (Oroxylum Indicum) Dalam Menghambat Pertumbuhan Bakteri Escherichia coli Metode Difusi Suwandi, Edy; Ramadhani, Natasya Intan; Wahdaniah, Wahdaniah
Jurnal Laboratorium Khatulistiwa Vol 3, No 2 (2020): Mei 2020
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v3i2.936

Abstract

Sebagian besar dari ribuan jenis tanaman yang ada di Indonesia dapat dimanfaatkan sebagai obat. Salah satunya yaitu tanman Bungli (Oroxylum Indicum) yang telah lama digunakan secara turun temurun di Asia Tenggara sebagai obat herbal untuk mengatasi tifus, batuk, hipertensi, panas dalam, demam dan diare. Kandungan senyawa alkaloid, flavonoid, saponin dan tannin pada tanaman ini dikenal memiliki aktifitas antiinflamasi, antirematik, antijamru dan antibakteri.Penelitian ini bertujuan untuk menjelaskna pengarus konsentrasi ekstrak metanol biji buah bungli (Oroxylum Indicum) dalam menhambat pertumbuhan balteri Eschericia Coli metode difusi cakram.Metode yang digunakan pada penelitian ini adalah eksperimental semu dengan teknik pengambilan sampel Purposive Sampling. Populasi dalam penelitian ini adalah biji buah bungli yang dibuat ekstrak. Sampel yang digunakan adalah biji buah bungli dengan konsentrasi 25%, 50% dan 75% yang masing masing konsentrasi dilakukan 9 kali pengenceran.Berdasarka hasil analisis statistik uji Regresi Linier didapatkan p value =0,000 < α 0,005 sehingga dinyatakan terdapat pengaruh konsentrasi ekstrak metanol biji buah bungl (oroxylum indicum) dalam menghambat pertumbuhan bakteri Eschericia Coli metode difusi. 
Edukasi Peran Zat Besi pada Pencegahan Anemia di SMP Negeri 06 Ketapang Dwisari, Fath; Dermawan, Abdurraafi' Maududi; Amalia, Puspa; Azmi, Khulul; Ramadhani, Natasya Intan; Purwanti, Desi Asih
Harmoni Sosial : Jurnal Pengabdian dan Solidaritas Masyarakat Vol. 2 No. 3 (2025): Juli : Harmoni Sosial : Jurnal Pengabdian dan Solidaritas Masyarakat
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62383/harmoni.v2i3.1809

Abstract

Anemia is a common health problem among adolescents, often caused by iron deficiency. This educational activity aimed to improve students' knowledge about the role of iron in preventing anemia. The program was conducted at SMP Negeri 06 Ketapang and involved 35 students as respondents. The results showed that after the educational session, the majority of students were in the moderate knowledge category (19 students or 54.3%), followed by the high category (7 students or 20%) and the low category (9 students or 25.7%). It can be concluded that this educational activity was quite beneficial in increasing students’ knowledge about the importance of iron in anemia prevention.
Identifikasi Bakteri Escherichia Coli Pada Ulkus Diabetikum Studi di Klink Kanazawa Pontianak Menggunakan PCR gen 16S rRNA Nurhidayatulloh, Ariffialdi; Ramadhani, Natasya Intan; Abidin, Khoirul Rista; Suwandi, Edy; Salsabila, Aisha; Widodo, Kaltri; Andriani, Lulu; Hamidah, Najwa; Wulandari, Tri
Jurnal Laboratorium Khatulistiwa Vol 9, No 1 (2025): November 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i1.1976

Abstract

Diabetes mellitus can lead to complications such as ulcers that are highly susceptible to bacterial infection. Escherichia coli is one of the pathogenic bacteria commonly found in diabetic foot ulcers. Accurate detection of E. coli species is essential to support appropriate infection management, and one of the reliable methods is Polymerase Chain Reaction (PCR) targeting the 16S rRNA gene. This study employed a descriptive design with an accidental sampling technique. Samples were obtained from swab specimens of diabetic foot ulcer patients. The procedures included culture on Eosin Methylene Blue (EMB) agar, Gram staining, bacterial DNA isolation, PCR amplification of the 16S rRNA gene, electrophoresis for visualization of PCR products, and sequencing for species confirmation. Based on the results of the study involving 30 diabetic foot ulcer patients at Kanazawa Clinic Pontianak, culture analysis showed that 4 isolates were positive for Escherichia coli. Gram staining further confirmed the presence of E. coli by demonstrating its characteristic morphology. The PCR results visualized through electrophoresis showed that 4 samples (13.3%) were positive for Escherichia coli based on the 16S rRNA gene, indicated by the presence of DNA bands at approximately 1500 bp. Sequencing analysis further confirmed the isolates as Escherichia coli with 99% similarity. 
Co-Authors Abidin, Khoirul Rista Amalia, Puspa Andriani, Lulu Azmi, Khulul Dermawan, Abdurraafi Maududi Hamidah, Najwa Nurhidayatulloh, Ariffialdi Purwanti, Desi Asih Salsabila, Aisha Suwandi, Edy Tri Wulandari Wahdaniah Wahyudi Widodo, Kaltri