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Aktivitas Peredaman Ekstrak Daun Pandan Wangi (Pandanus amaryllifolius) dengan Metode DPPH (2,2-difenil-1-pikrilhidrazil) Nurhasanah, Devika; Nofran Putra Pratama; Sri Purwa Pujihastuti
JOURNAL OF PHARMACEUTICAL (JOP) Vol 1 No 2 (2023): Journal of Pharmaceutical (JOP)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) UNIVERSITAS JENDERAL ACHMAD YANI YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/jop.v1i2.1223

Abstract

Background: Antioxidants are compounds that prevent an oxidation reaction from free radical compounds and important role in capturing free radicals in the human body. Excessive free radicals in the body can cause tissue damage and degenerative diseases. Pandanus amarillyfolius are known to act as natural antioxidant that have the potential to capture free radical compounds, because of their presence of flavonoid compounds. Objective: This study aims to determine the free radical scavenging activity of DPPH against the leaf extract of Pandanus amaryllifolius. Method: Pandanus amaryllifolius extraction was carried out using the maceration method with methanol as solvent (1:10). Concentration was carried out using a rotary evaporator to obtain a thick extract. Phytochemical screening and TLC test were carried out qualitatively using the mobile phase n-butanol: acetic acid: water (6:2:2) and phytochemical testing including flavonoids, alkaloids, saponins, tannins, steroids or terpenoids to determine the presence of flavonoid compounds as antioxidants. Free radical scavenging activity was tested using DPPH free radical scavenger with concentrations of vitamin C 2, 4, 5, 6, 8 ppm and pandanus fragrance extract concentrations of 10, 15, 20, 25 ppm and measured using a UV-Vis spectrophotometer to calculate the IC50 value. Result: The results of the phytochemical screening test of fragrant pandan indicate the presence of flavonoid compounds. The Rf value of the TLC test obtained 0.765 quarcetin and 0.787 extract and the resulting yellow spots, the Rf value of pandan extract which was almost the same as the Rf value of quercetin indicated that the pandan extract may contain the same compounds as quercetin, namely flavonoid compounds. The results of the free radical scavenging activity of methanol extract of pandan leaves were 86,861 g/ml and for comparison, vitamin C was 39,103 g/ml. Based on the statistical analysis of the T-Test, it is known that there is a difference between the sample and the standard with a significant value (p <0.05), namely 0.001 and 0.023. Conclusion: The Pandanus amaryllifolius extract test using the DPPH method is in the strong category (IC50 <50 -100 g/ml) and the comparison of Vitamin C is in the very strong category (IC50 <50 g/ml).
The Effect of Ethanol Concentration Variations on The Total Phenolic And Flavonoid Levels of Bauhinia purpurea L. Leaf Extract Nurhasanah, Devika; Ulvia, Rengganis; Junita, Fina
Journal of Biotechnology and Natural Science Vol. 4 No. 2 (2024): December
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v4i2.12060

Abstract

Bauhinia purpurea L. is an ornamental plant that is not widely known or explored by the public, despite its significant antioxidant properties. Phenolic compounds and flavonoids, known for their antioxidant activity, can be extracted from the leaves through maceration with 70% ethanol and 96% ethanol. The difference in solvent polarity between these two ethanol concentrations affects the solubility of bioactive compounds. This study aimed to evaluate the effect of 70% and 96% ethanol concentrations Bauhinia purpurea L on the total phenolic and flavonoid levels in. An experimental method was employed, using Bauhinia purpurea L powder macerated with 70% and 96% ethanol, followed by evaporation at 40°C-50°C to obtain thick extracts. The extracts were subjected to organoleptic tests, phytochemical screening, Thin Layer Chromatography (TLC), and UV-Vis spectrophotometry. Total phenolic content was determined using gallic acid standards (mg GAE/g extract), while total flavonoid content was measured using quercetin standards (mg QE/g extract). The organoleptic test revealed that both the 70% and 96% ethanol extracts of Bauhinia purpurea L had a blackish-green color, thick consistency, distinctive odor, and bland taste. Phytochemical screening indicated the presence of phenolics, flavonoids, and saponins. The total phenolic content for the 70% ethanol extract was 14.644 ± 0.222 mg GAE/g, and the total flavonoid content was 25.519 ± 0.921 mg QE/g. The total phenolic content for the 96% ethanol extract was 7.176 ± 0.347 mg GAE/g, and the total flavonoid content was 11.208 ± 0.412 mg QE/g. These results indicate a significant difference between the use of 70% and 96% ethanol in extracting total phenolic and flavonoid content from Bauhinia purpurea L, with the 70% ethanol extract showing higher levels of both compounds.
Penyuluhan Terkait Penyakit Tidak Menular di LPK Cempaka Bantul Indra, Kholif Sholehah Indra K; Nurhasanah, Devika; Andriani, Yuni
The Journal of Innovation in Community Empowerment Vol 5 No 1 (2023): Journal of Innovation in Community Empowerment (JICE)
Publisher : Universitas Jenderal Achmad Yani Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/jice.v5i1.840

Abstract

Penyakit tidak menular (PTM) menjadi salah satu penyebab utama kematian di dunia. Hasil riset kesehatan dasar (RISKESDAS) menunjukkan bahwa kasus terbanyak dari penyakit tidak menular adalah diabetes melitus (DM), Hipertensi dan penyakit terkait kardiovaskuler. Perubahan gaya hidup merupakan salah satu program dalam pengendalian penyakit tidak menular yang difokuskan pada faktor risiko. Faktor risiko penyakit tidak menular dapat dicegah dan dikendalian lebih dini, sehingga diperlukan pengetahuan dan informasi terkait penyakit tidak menular dalam melakukan perubahan. Faktor risiko penyakit tidak menular membutuhkan waktu yang relative lama terutama pola gaya hidup. Salah satu merupakan peran serta masyarakat dalam pengendalian PTM adalah melalui Gerakan masyarakat hidup sehat (GERMAS). Germas merupakan suatu Gerakan nasional sebagai upaya promotive dan preventif. Tahapan persiapan terdiri dai observasi, pelaksanaan, evaluasi dan pelaporan. Tahapan persiapan dimulai dari observasi lapangan, penyusunan proposal, dan persiapan materi. Tahapan pelaksanaan yaitu pre-test, penyuluhan materi, dan diskusi. Sedangkan tahapan evaluasi terdiri dari post-test, dan penyusunan laporan. Hasil dari nilai pre-test yakni mempunyai rata-rata nilai 53,78% menunjukkan masih kurangnya pengetahuan penyakit tidak menular. Nilai rata-rata setelah mendapatkan edukasi yakni 78,65%. Berdasarkan hasil tersebut maka pengabdian kepada masyarakat ini mampu meningkatkan pengetahuan kepada masyarakat LKS Cempaka Bantul.
Edukasi Kesehatan Pada Masyarakat Di Masa Pandemi Covid-19: Aspek Penting Protokol Kesehatan dan Pengenalan DaGuSiBu Nurhasanah, Devika; Nurhayati, Yuni; Pramita, Gandi Ais
The Journal of Innovation in Community Empowerment Vol 5 No 2 (2023): Journal of Innovation in Community Empowerment (JICE)
Publisher : Universitas Jenderal Achmad Yani Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/jice.v5i2.921

Abstract

Corona virus disease 2019 (Covid-19) merupakan penyakit menular yang menyerang sistem pernapasan dan dinyatakan sebagai pandemi di tahun 2020. Pada awal pandemi Covid-19 masyarakat belum mengetahui cara mencegah penyebaran serta cenderung lebih konsumtif dalam penggunaan obat-obatan yang dapat membantu meningkatkan imunitas atau mengurangi gejala ringan dari virus Covid-19. Berdasarkan hal tersebut, perlu dilakukan edukasi terhadap masyarakat yakni melalui penyuluhan tentang pentingnya menerapkan protokol Kesehatan (prokes), dengan 5M serta penyuluhan DaGuSiBu. Pengabdian masyarakat ini bertujuan meningkatkan pengetahuan masyarakat tentang aspek penting prokes dan pengenalan DaGuSiBu. Sasaran kegiatan ini adalah Ibu-ibu PKK Padukuhan Ngireng-ireng, Panggungharjo, Sewon, Bantul. Evaluasi yang dilakukan melalui perbandingan nilai pretest dan posttest. Hasil pretest dan posttest kemudian dianalisis secara statistik. Berdasarkan hasil analisisi statistik, penyuluhan berpengaruh secara signifikan (p<0,05) terhadap peningkatan pengetahuan Ibu-ibu PKK mengenai aspek penting Prokes dari 50% menjadi 93,75% dan mengenai DaGuSiBu dari 53,33% menjadi 86,67% sesudah mengikuti penyuluhan. Selain itu, usia tidak berhubungan (p>0,05) dengan peningkatan pengetahuan tentang prokes dan pengenalan DaGuSiBu pada ibu-ibu PKK Padukuhan Ngireng-ireng, Panggungharjo, Sewon, Bantul
Pengaruh Sosialisasi Pengelolaan Sampah Organik dan Anorganik di Desa Jetakan Sumberagung Bantul Nurhasanah, Devika; Kurniasih, Kholif Sholehah Indra
The Journal of Innovation in Community Empowerment Vol 5 No 2 (2023): Journal of Innovation in Community Empowerment (JICE)
Publisher : Universitas Jenderal Achmad Yani Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/jice.v5i2.964

Abstract

Sampah merupakan material sisa yang dihasilkan dari kegiatan dan usaha manusia. Permasalahan sampah tidak dapat dipisahkan dari kehidupan manusia, karena setiap beraktivitas berpotensi menghasilkan sampah. Pengelolaan sampah adalah suatu kegiatan yang berkesinambung dalam pengurangan dan penanganan sampah. Pemisahan sampah organik dan anorganik merupakan salah satu cara dalam penanganan sampah, terutama sampah dosmetic. Tujuan dari pengabdian kepada masyarakat ini adalah untuk meningkatkan pengetahuan masyarakat mengenai pengelolaan sampah organik maupun anorganik. Pengabdian masyarakat dilakukan di Desa Jetakan, Kecamatan Sumberagung, Kabupaten Bantul Yogyakarta dengan sasaran kegiatan ini adalah ibu-ibu rumah tangga. Metode yang dilakukan dalam pengabdian ini adalah sosialisasi menggunakan media leaflet. Pengukuran hasil kegiatan melalui analisis kuisioner terkait pemahaman mengenai persampahan. Dari hasil analisis kegiatan sosialisasi pengelolaan sampah organik dan anorganik menunjukkan terjadi peningkatan pemahaman masyarakat terhadap persampahan.
Aktivitas Peredaman Ekstrak Daun Pandan Wangi (Pandanus amaryllifolius) dengan Metode DPPH (2,2-difenil-1-pikrilhidrazil) Nurhasanah, Devika; Nofran Putra Pratama; Sri Purwa Pujihastuti
JOURNAL OF PHARMACEUTICAL (JOP) Vol. 1 No. 2 (2023): Journal of Pharmaceutical (JOP)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) UNIVERSITAS JENDERAL ACHMAD YANI YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/jop.v1i2.1223

Abstract

Background: Antioxidants are compounds that prevent an oxidation reaction from free radical compounds and important role in capturing free radicals in the human body. Excessive free radicals in the body can cause tissue damage and degenerative diseases. Pandanus amarillyfolius are known to act as natural antioxidant that have the potential to capture free radical compounds, because of their presence of flavonoid compounds. Objective: This study aims to determine the free radical scavenging activity of DPPH against the leaf extract of Pandanus amaryllifolius. Method: Pandanus amaryllifolius extraction was carried out using the maceration method with methanol as solvent (1:10). Concentration was carried out using a rotary evaporator to obtain a thick extract. Phytochemical screening and TLC test were carried out qualitatively using the mobile phase n-butanol: acetic acid: water (6:2:2) and phytochemical testing including flavonoids, alkaloids, saponins, tannins, steroids or terpenoids to determine the presence of flavonoid compounds as antioxidants. Free radical scavenging activity was tested using DPPH free radical scavenger with concentrations of vitamin C 2, 4, 5, 6, 8 ppm and pandanus fragrance extract concentrations of 10, 15, 20, 25 ppm and measured using a UV-Vis spectrophotometer to calculate the IC50 value. Result: The results of the phytochemical screening test of fragrant pandan indicate the presence of flavonoid compounds. The Rf value of the TLC test obtained 0.765 quarcetin and 0.787 extract and the resulting yellow spots, the Rf value of pandan extract which was almost the same as the Rf value of quercetin indicated that the pandan extract may contain the same compounds as quercetin, namely flavonoid compounds. The results of the free radical scavenging activity of methanol extract of pandan leaves were 86,861 g/ml and for comparison, vitamin C was 39,103 g/ml. Based on the statistical analysis of the T-Test, it is known that there is a difference between the sample and the standard with a significant value (p <0.05), namely 0.001 and 0.023. Conclusion: The Pandanus amaryllifolius extract test using the DPPH method is in the strong category (IC50 <50 -100 g/ml) and the comparison of Vitamin C is in the very strong category (IC50 <50 g/ml).
Uji Aktivitas Antibakteri Infusa Kayu Secang (Caesalpinia Sappan L.) Terhadap Bakteri Escherichia coli ATCC 25922 dan Staphylococcus aureus ATCC 25923 Leswara, Dianita Febrina; Nurhasanah, Devika; Retno P., Maysi
JOURNAL OF PHARMACEUTICAL (JOP) Vol. 2 No. 2 (2024): Journal of Pharmaceutical (JOP)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) UNIVERSITAS JENDERAL ACHMAD YANI YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/jop.v2i2.1460

Abstract

Background: Escherichia coli and Staphylococcus aureus are two common types of bacteria that can cause infections in the human body. The treatment to overcome infections caused by Escherichia coli and Staphylococcus aureus bacteria is by administering antibiotics. The use of antibiotics that are not appropriate or not according to instructions can cause resistance so that alternative antibacterial agents from herbal plants begin to be developed. One of the plants known to contain antibacterial compounds is the secang plant. The secang wood stem is known to contain flavonoid and tannin antibacterial compounds.  Objective: To determine the bacterial activity and Minimum Inhibitory Concentration (MIC) of secang wood infusa (Caesalpinia sappan L.) against Escherichia coli and Staphylococcus aureus. Method: Extraction of active compounds in secang wood was done by infundation method. The concentration of secang wood infusion that will be tested is 25%, 50%, 75% and 100%. Testing the antibacterial activity of secang wood infusion was carried out against Escherichia coli and Staphylococcus aureus using the well diffusion method. Results: Extraction of active compounds in secang wood is carried out using the infundation method. All concentrations of secang wood infusion tested 25%, 50%, 75% and 100%, showed antibacterial activity against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923. The smallest concentration that showed inhibition of antibacterial activity was at a concentration of 25% with diameter of of inhibition zone is 20.52 mm in Escherichia coli ATCC 25922 and 20.58 mm in Staphylococcus aureus ATCC 25923 bacteria. Conclusion: Secang wood infusion has antibacterial activity with Minimum Inhibitory Concentration (MIC) at 25% concentration with strong inhibition
Potensi Antioksidan Ekstrak Metanol Fuli Pala (Myristica fragrans Houtt) Djamaluddin, Rosmiyati R; Putra Pratama, Nofran; Nurhasanah, Devika
JOURNAL OF PHARMACEUTICAL (JOP) Vol. 2 No. 1 (2024): Journal of Pharmaceutical (JOP)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) UNIVERSITAS JENDERAL ACHMAD YANI YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/jop.v2i1.1517

Abstract

Background : The mace is the part that surrounds the nutmeg seeds. Mace and nutmeg seeds are widely used as a spice in food products and in traditional medicine used as a remedy for stomach pain, analgesics, stimulants. Nutmeg mace also contains flavonoids and phenolics that have potential as antioxidants. Research Objective: Determine the antioxidant activity of methanol extract of nutmeg mace (Myristica fragrans Houtt) in reducing free radicals DPPH Research Method: Nutmeg macaques are extracted using methanol solvents through a maceration process (1:10), then the extracts obtained are tested, namely organoleptic tests, moisture content tests, phytochemical tests, KLT tests, and antioxidant tests using quercetin comparators with varying concentrations, namely 2 ppm, 4 pmm, 6 ppm, 8 ppm, and 10 ppm Research Results: The results of the organoleptis test showed that the methanol extract of nutmeg mace had a deep black color, a distinctive aroma, and a thick texture. 0.83% for moisture content test results. The results of phytochemical screening showed that nutmeg mace methanol extract was positive for flavonoids, phenolics, alkaloids, saponins, and tannins. The KLT test also showed positive results containing flavonoids. The free radical scavenging activity of DPPH from metanol extract of nutmeg mace showed an IC50 value of 13.781 ppm ±SD 0.247 and a standard IC50 value of 4.181 ppm ±SD 0.247 compared to quercetin. Based on statistical analysis, there was no significant difference because the data obtained was 0.074 > 0.05 Conclusion: Methanol extract of nutmeg mace has antioxidant activity in reducing DPPH free radicals and shows an IC50 value of 13.781 ±0,211 ppm which is included in the category of very strong.
Penentuan Kadar Fenolik Total Dan Peredaman Radikal Bebas Ekstrak Metanol Daun Bayam Hijau (Amaranthus hybridus L) Dengan DPPH Nurhasanah, Devika; Mafa , Sutri Hud; Pratama, Nofran
JOURNAL OF PHARMACEUTICAL (JOP) Vol. 2 No. 2, Special Edition (2024): Journal of Pharmaceutical (JOP)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) UNIVERSITAS JENDERAL ACHMAD YANI YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/jop.v2i2, Special Edition.1477

Abstract

Background: Free radicals are molecules that have one or more free or unpaired electrons, so that free radicals are unstable. Free radicals can be inhibited in the presence of antioxidant compounds. The compound antioxidant can be obtained from various sources, one of which is from the green spinach plant. Amaranthus hybridus L. is a herbaceous plant from the Amaranthaceae family that contains natural antioxidant compounds, namely phenolic compounds. Objective: This study aimed to determine the total phenolic levels contained in methanol extract of Amaranthus hybridus L. and what is the value of IC50 methanol extract of Amaranthus hybridus L. which is able to reduce DPPH free radicals. Method: Amaranthus hybridus L. were extracted with methanol solvent by maceration method in a ratio (1:10). Then a qualitative analysis was carried out in the form of phytochemical screening, identification of phenolic compounds by Thin Layer Chromatography (TLC) method using the methanol mobile phase: chloroform: n-hexane (1:9:1 v/v/v) and the stationary phase of silica gel GF254. Quantitative analysis of the total phenolic content test, and free radical suppression activity test by DPPH method which was measured using a UV-Vis spectrophotometer to calculate IC50. Result: Methanol extract of Amaranthus hybridus L. positively contains alkaloid compounds, phenolics, flavonoids, saponins, and tannins. The TLC Rf quercetin value is 0.475, and the Rf extract is two spots namely 0.35 and 0.837. Total phenolic content of 1.150 ± 0.025% and a free radical suppression activity of IC50 value of 14.786 ppm. Conclusion: Methanol extract of Amaranthus hybridus L. could be categorized as very strong.
The Effect of Extraction Time Variation on Caffeine Content in Robusta Coffee Extract using Ultrasound-Assisted Extraction Method Anggraini, Amelia Putri; Fildzah Arifah, Mitsalina; Sayyidah Yahya , Fina Ummu; Nurhasanah, Devika
Journal of Food and Pharmaceutical Sciences Vol 13, No 1 (2025): J.Food.Pharm.Sci
Publisher : Institute for Halal Industry and System (IHIS) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.18750

Abstract

West Lampung Robusta coffee has a higher caffeine content than other types of coffee. The variations in extraction time in ultrasound-assisted extraction (UAE) produced different caffeine contents than conventional extraction methods. This study aims to determine extraction time variation (15, 30, 45) influences caffeine contents in roasted coffee beans. The organoleptic test and alkaloid test evaluated Robusta coffee qualitatively. The extract obtained was measured by measuring the % MC, yield value, and wavelength scanning. Quantitative analysis using UV-Vis Spectrophotometry determined the caffeine content with different extraction times, and then each group was assessed using SPSS software. The coffee bean powder was dark brown, had a distinctive coffee aroma, bitter taste, and smooth texture, and contained alkaloids with the Mayer, Wagner, and Dragendorff tests. The yield and % water content of extract at 30 (36.938%; 6.39%) were higher than at 15 (24.111%; 6.07%) and 45 minutes (22.170%; 6.37%). Alkaloids of the extract were investigated at a wavelength of 273 nm. Caffeine contents were 0.977 ± 0.018 mgCE/mg at the 15, 0.792 ± 0.054 mgCE/mg at the 30, and 0.979 ± 0.026 mgCE/mg at the 45 minutes in significant (P<0.05). In conclusion, the difference in extraction time impacted the caffeine content of the robusta coffee bean.