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All Journal Jurnal Nutrisi Ternak Tropis (JNT) Media Farmasi Jurnal Ilmiah Manuntung: Sains Farmasi dan Kesehatan
Tri Puji Lestari
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Bobot Karkas dan Persentase Organ Itik Magelang Jantan (Anas Platyrhynchos) yang Diberi Suplemen Inulin Pada Ransumnya Lilis Hartati; Galy Hardyta; Tri Puji Lestari; Rizki Nur Amin
Jurnal Nutrisi Ternak Tropis Vol. 7 No. 1 (2024): JNT Jurnal Nutrisi Ternak Tropis Maret
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jnt.2024.007.01.4

Abstract

Itik Magelang merupakan salah satu jenis unggas dwiguna yang mampu menghasilkan daging dan telur. Optimalisasi produktivitas itik dapat dilakukan dengan memberikan imbuhan pakan (feed aditif) berupa prebiotik. Penelitian ini bertujuan untuk mengkaji pengaruh penambahan inulin dalam pakan terhadap bobot karkas dan persentase organ dalam Itik Magelang jantan. Rancangan percobaan pada penelitian ini adalah Rancangan Acak Lengkap (RAL). Penelitian ini menggunakan itik Magelang jantan sebanyak 100 ekor. Penelitian ini terdiri dari 4 perlakuan dengan 5 ulangan. Perlakuannya adalah: P0 (pakan control) P1(pakan + Inulin 0,5%) P2 (pakan + Inulin 1%) P3 (Pakan + Inulin 1,5%) dengan jumlah itik ulangan adalah 5 ekor. Variabel yang diamati meliputi: bobot karkas, dan persentase organ dalam (hati, jantung, ampela, dan Limpa). Analisis data menggunakan analisis ragam (ANOVA) dan data yang berbeda nyata diuji lanjut Duncan's Multiple Range (DMRT). Hasil penelitian ini menunjukkan bahwa penambahan inulin pada pakan tidak berpengaruh nyata terhadap bobot karkas dan persentase organ dalam. Bobot karkas (899,35 ± 92,39) persentase jantung (0,87 ± 0,52%) persentase hati (2,70 ± 0,35%) persentase ampela (4,36 ± 0,47%) persentase limpa (0,11 ± 0,05%) yang masih ke dalam kondisi normal. Berdasarkan hasil penelitian dapat disimpulkan bahwa penambahan prebiotik inulin sebanyak 0, 0,5, 1, dan 1,5% dalam pakan itik Magelang jantan tidak berpengaruh nyata terhadap bobot karkas dan persentase organ dalam (jantung, hati, ampela, dan limpa).
Validasi Penetapan Kadar Kuersetin Ekstrak Etanol Daun Kemangi (Ocimum sanctum L) Secara Kromatografi Cair Kinerja Tinggi (KCKT) Kurniawati, Evi; Tri Puji Lestari; Esti Ambar Widyaningrum
Media Farmasi Vol 20 No 1 (2024): Media Farmasi Edisi April 2024
Publisher : Jurusan Farmasi Poltekkes kemenkes Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32382/mf.v20i1.458

Abstract

Validation of Determination of Quercetin Content of Ethanol Extract of Basil Leaves (Ocimum sanctum L) by High-Performance Liquid Chromatography (HPLC) One of the Indonesian plants that has medicinal properties is the basil plant (Ocimum sanctum L). There are several active substances contained in basil leaves, such as the flavonoid compound quercetin, which is known to be found in basil. Quercetin has several very useful biological activities such as antibacterial, anticancer, antiviral, and anti-inflammatory. Determination of quercetin content in basil leaves requires a valid method. The selection of an analytical method that has good specificity, linearity, accuracy and precision is a very important aspect in order to obtain acceptable results. The aim of this research is to validate the determination of quercetin levels contained in the ethanol extract of basil leaves (Ocimum sanctum L) using high-performance liquid chromatography (HPLC). The HPLC system used is the Shimadzu DGU-20A5R type with a Uv-Vis detector at a wavelength of 374 nm. The stationary phase used was a C18 column with a mobile phase of a mixture of methanol: aqua bi destillate in a ratio of 59:41 which flowed isocratically at a rate of 1.2 mL/minute. Assay validation parameters include selectivity, linearity, precision, and accuracy tests. The research results showed that the quercetin method was validated with all parameters meeting the requirements. The linearity test produces a correlation value (r) = 0.9919, good selectivity, precision produces an RSD of less than 2% and accuracy produces a recovery percentage in the range of 98-102%. The average quercetin content in the ethanol extract of basil leaves is 12.70% w/w. Salah satu tanaman Indonesia yang berkhasiat sebagai obat adalah tumbuhan kemangi (Ocimum sanctum L). Terdapat beberapa kandungan zat aktif dalam daun kemangi seperti senyawa flavanoid kuersetin diketahui terdapat dalam kandungan kemangi. Kuersetin memiliki beberapa aktivitas biologi yang sangat bermanfaat seperti antibakteri, antikanker, antivirus, dan antiinflamasi. Penentuan kadar kuersetin dalam daun kemangi membutuhkan metode yang valid. Pemilihan metode analisis yang memiliki spesifitas, linieritas, akurasi dan presisi yang baik merupakan aspek yang sangat penting agar diperoleh hasil yang dapat diterima. Tujuan dari penelitian ini adalah untuk melakukan validasi penetapan kadar kuersetin yang terkandung dalam ekstrak etanol daun kemangi (Ocimum sanctum L) secara kromatografi cair kinerja tinggi (KCKT). Sistem KCKT yang digunakan adalah tipe Shimadzu DGU-20A5R dengan detektor Uv-Vis pada panjang gelombang 374 nm. Fase diam yang digunakan adalah kolom C18 dengan fase gerak campuran methanol : aqua bidestilata pada perbandingan 59:41 yang dialirkan secara isokratik pada laju 1,2 mL/menit. Parameter validasi metode penetapan kadar kuersetin meliputi uji selektivitas, linieritas, presisi dan akurasi. Hasil penelitian menunjukkan bahwa metode KCKT yang digunakan tervalidasi yang ditunjukkan dengan semua parameter uji memenuhi syarat yang ditetapkan. Uji linieritas menunjukkan nilai korelasi (r) = 0,9919, selektivitas baik, presisi menghasilkan RSD kurang dari 2% dan akurasi menghasilkan persen rekoveri pada kisaran 98-102%. Rata-rata kandungan kuersetin dalam ekstrak etanol daun kemangi adalah 12,70% b/b.
UJI STABILITAS DAN UJI HEDONIK MASKER GEL PEEL-OFF EKSTRAK DAUN BINAHONG (Anredera cordifolia (Ten.) Steenis) DENGAN VARIAN KONSENTRASI POLIVINIL ALKOHOL (PVA) SEBAGAI FILMING AGENT Tri Puji Lestari; Anisa Riana Putri; Ida Kristianingsih; Evi kurniawati; Fita Sari
Jurnal Ilmiah Manuntung Vol 8 No 2 (2022): Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51352/jim.v8i2.639

Abstract

Research on the Formulation Physical Stability Test and hedonic test of the peel-off gel mask of binahong Leaf Extract has been conducted. This study aims to determine the best formula of the binahong Leaf Extract peel-off gel mask preparation with PVA as a filming agent. The steps of the procedure in this research include plant determination, sample preparation, making binahong leaves simplicia, making binahong leaves extract, formulation of peel off gel mask evaluation of physical properties, stability test, irritation test and hedonic test. Formulation of binahong Leaf Extract peel-off gel mask were carried out in three formulas with various variations of PVA concentrations of 5%, 5,5%, and 6%. The results of the physical properties test shows that peel-off extract masks of binahong leaves with different concentration variations differ in organoleptic, ph test, and drying time test a statistical data analysis. Based on the results of the stability test using the cycling test method with temperature ± 4̊ C and ± 40̊ C over the course of six cycles. he most stable results of existing tests are F2 and F3 showed no significant differences in each evaluation. Test irritation suggests that the gel peel-off mask was not irritating. From the hedonic test results, panelists prefer F3 compared to other formulas.
AKTIVITAS ANTIBAKTERI DARI SEDIAAN GEL EKSTRAK TERPURIFIKASI KELOPAK ROSELLA TERHADAP BAKTERI Escherichia coli Fita Sari; Ida Kristianingsih; Tri Puji Lestari; Fathul Hidayatul; Yuyun Wulandari
Jurnal Ilmiah Manuntung Vol 9 No 1 (2023): Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51352/jim.v9i1.674

Abstract

Natural materials had various types of primary and secondary metabolite compounds that were useful for treating a disease. Plant materials that contain high levels of secondary metabolites could be developed into raw materials for traditional medicines. An example of the development of natural medicine which was in high demand is making traditional medicinal raw materials which have antibacterial properties. These could add to the type of therapy for a disease with smaller side effects. The aim of this study was to determine the activity of the purified extract gel preparation of rosella petals as an antibacterial. The extract was obtained from the maceration method which was then followed by purification. The antibacterial activity test in this study used the well diffusion method. Detection of metabolites by Thin Layer Chromatography (TLC) includes the stationary phase of silica GF254 and the mobile phase Toluene: Acetic acid: Formic acid (6:6:1). The concentration of purified extract of rosella petals for the formulation was 5, 15 and 25%. Data analysis was carried out with the One Way Anova test with a 95% confidence level. The results of the research that the purified extract gel preparation of rosella petals has the potential to inhibit the growth of Escherichia coli bacteria in the sensitive category at a concentration of 25%. The results of a positive phytochemical screening for flavonoid compounds were indicated by the formation of a red-orange color in the test and identification using TLC produced an Rf of 0.85 which has similarities with previous studies. The conclusion of this study was that the ETKR gel preparation with a concentration of 25% was sensitive as an antibacterial
Co-Authors Anisa Riana Putri Esti Ambar Widyaningrum Evi Kurniawati Evi Kurniawati, Evi fathul hidayatul Fita Sari Hardyta, G. Ida Kristianingsih Lilis Hartati Rizki Nur Amin Yuyun Wulandari