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Supplementation of Rumen By Pass Protein-Fat: Effect on Feed Intake, Nutrient Digestibility and The Profile of Duodenal Digesta Fatty Acids Hartati, Lilis; Sumantri, Ika; Agus, Ali
ANIMAL PRODUCTION Vol 16, No 2 (2014): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (388.53 KB)

Abstract

Abstract.  The aim of this research was to study the effects of rumen by pass protein-fat supplementation (RBPF) on feed intake, nutrients digestibility and profile of duodenal digesta of fatty acids. Two rumen fistulated and duodenum canulated dairy cows were fed ration that consisted of king grass and concentrate (60:40). The concentrate was supplemented with 20% RBPF. The feeding trial was conducted for four weeks in which the first week was the control period, the second week was the adaptation period, the third week was the early collection period and finally the fourth week was the late collection period. The samples of duodenal digesta were collected 1 hour before morning feeding and 1, 3 and 5 hours after morning feeding. The cows were fed twice a day and concentrate was offered before forage. The results showed that in comparison to the control, supplementation of RBPF increased nutrients intake of dry matter (DM) (6.95 vs. 6.27%), organic matter (OM) (6.20 vs. 5.70%), crude fat (CF) (0.49 vs. 0.41%), crude fiber (CFb) (1.84 vs. 1.49%) and nitrogen free extract (NFE) (3.23 vs. 3.03%), while the crude protein (CP) intake decreased (0.64 vs. 0.77%). Supplementation of RBPF improved the nutrients digestibility of dry matter (67.24 vs. 62.15%), organic matter (68.71 vs. 65.29%), crude fiber (66.20 vs. 57.46%) and nitrogen free extract (72.37 vs. 66.47%). RBPF supplementation also increased linoleic acid content in duodenal digesta sample at 1and 3 hours after feeding. In conclusion, RBPF supplementation did not negatively affect feed intake and nutrient digestibility. RBPF also increased the proportion of linolenic acid, eicosapentaenoic acid (EPA) and docosahexsaenoic acid (DHA) in duodenal digesta until 3 hours after feeding. Key words: RBPF, supplementation, feed intake, nutrient digestibility, fatty acid Abstrak.  Penelitian ini bertujuan untuk mengetahui pengaruh  suplementasi protein-lemak by pass rumen terhadap konsumsi, kecernaan nutrien serta profil asam lemak pada digesta duodenum sapi perah. Dua ekor sapi perah berfistula rumen dan berkanula duodenum diberi pakan hijauan dan konsentrat dengan rasio 60:40, diberi suplemen protein-lemak by pass rumen 20% dari konsentrat.  Percobaan dilakukan selama 4 minggu, minggu pertama adalah periode kontrol, minggu ke-2 periode adaptasi, minggu ke-3 periode koleksi awal dan minggu ke-4 periode koleksi akhir. Sampel digesta duodenum diambil 1 jam sebelum pemberian pakan dan 1; 3 serta 5 jam setelah pemberian pakan. Ransum diberikan dua kali sehari dengan konsentrat lebih dulu sebelum hijauan. Dibanding periode kontrol, suplementasi protein lemak by pass rumen sebanyak 20% menaikkan konsumsi bahan kering (BK) (6,95 vs 6,27%), bahan organik (BO) (6,20 vs 5,70%), lemak kasar (LK) (0,49 vs 0,41%), serat kasar (SK) (1,84 vs 1,49%) dan bahan ekstrak tanpa nitrogen (BETN) (3,23 vs 3,03%) tetapi menurunkan konsumsi protein kasar (PK) (0,64 vs 0,77%). Suplementasi protein lemak by pass rumen juga memberikan kecernaan BK, BO, SK dan BETN ( 67,24 vs 62,15%; 68,71 vs 65,29%; 66,20 vs 57,46%; 72,37 vs 66,47%) yang lebih tinggi dibanding kontrol. Suplementasi lemak  by pass rumen juga menaikkan proporsi asam lemak linolenat pada jam ke-1 dan ke-3 setelah makan. Pemberian protein-lemak by pass rumen dalam ransum tidak berpengaruh negatif terhadap konsumsi dan kecernaan nutrien pakan serta meningkatkan proporsi asam lemak linoleat, EPA (eicosapentaenoic acid) dan DHA (docosahexsaenoic acid) digesta duodenum. Kata kunci: protein lemak by pass rumen, suplementasi, konsumsi, kecernaan nutrien, asam lemak
In Vitro Digestibilities of Six Rumen Protected Fat-Protein Supplement Formulas Hartati, Lilis; Agus, Ali; Widyobroto, BP; Yusiati, LM
ANIMAL PRODUCTION Vol 14, No 1 (2012): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Abstract. The aim of the research was to evaluate the efficacy of protection method of rumen protected fat-protein supplements. In vitro digestibility test was carried out to examine nutrients digestibility of different supplement formula based on the sources of protein and oil. The research used two sources of fat namely crude palm oil (CPO) and fish oil (FO) and three sources of protein namely milk skim, soy flour, and soybean meal. Thus there were 6 combinations that subjected in the in vitro digestibility test. The observed variables were the digestibility of dry matter (DM), organic matter (OM), crude fat (CF), and crude protein (CP). Results indicated that the method for protecting protein and fat was effective. This was showed by low nutrients digestibility in the rumen and high nutrients digestibility in the post rumen. In conclusion the combination between skim milk and CPO gave the best results among the other supplement formula.Keywords: rumen protected nutrient, fat-protein supplement, rumen digestibility, in vitroAnimal Production 14(1):1-5, January 2012
Free Fatty Acid Concentration and Carboxy methyl cellulase Activity of Some Formulas of Protected Fat-proteins Tested In Vitro Hartati, Lilis; Agus, Ali; Yusiati, Lies Mira; Widyobroto, Budi Prasetyo
ANIMAL PRODUCTION Vol 17, No 2 (2015): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (550.311 KB) | DOI: 10.20884/1.anprod.2015.17.2.513

Abstract

The aim of this study was to determine the levels of free fatty acids and carboxymethylcellulase activity (cmc-ase) activity of some protected fat-proteins base on in vitro Tilley and Terry method. Two sources of fat, i.e. crude palm oil and fish oil and three sources of protein i.e. skim milk, soybean flour and soybean meal were used in the formulation of protected fat-protein, and thus there were six treatment combinations. The filtrate from the in vitro test was analyzed for the levels of free fatty acids and  cmcase activity. The result of this research indicates that different combinations of feed materials and fat give different content of free fatty acid in first stage and second stage in vitro, with the best results in the combination treatment of skim milk and palm oil that give the lowest result of  free fatty acid concentration in fisrt stage in vitro (0.168%) and the highest result free fatty acid concentration in second stage in vitro ( 4.312%) . The activity of CMC-ase was not influenced by different  sources of fat and protein. It can be concluded was that the protection of the combination between skim milk and CPO gives the highest protection results.
Pengaruh Penambahan Probiotik Mix Culture Pada Fermentasi Anaerob Kulit Kopi Terhadap Kadungan Serat Kasar, Dan Protein Kasar Mohamad Haris Septian; lilis Hartati; Rahma Wulan Idayanti; ismiana Kholifatun
Agrivet : Jurnal Ilmu-Ilmu Pertanian dan Peternakan (Journal of Agricultural Sciences and Veteriner) Vol. 10 No. 2 (2022): Desember
Publisher : Fakultas Pertanian Universitas Majalengka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31949/agrivet.v10i2.3558

Abstract

This research aim to determine the effect of addition probiotic mixculture on the anaerobic fermentation of coffee peel on the content of dry matter, crude fiber, and crude protein. This research conducted by experimentally using a Complete Randomized Design (CRD) with 4 treatments and 5 repeats. The treatment was P1 (coffee peel + 3% molasses), P2 (coffee peel + 3% molasses + 0.5% Heryaki probiotic), P3 (coffee peel + 3% molasses + 1% Heryaki probiotic), P4 (coffee peel + 3% molasses + 1.5% Heryaki probiotic). The results showed that the addition of probiotic mix culture had no significant effect (P>0.05) on crude fiber and crude protein. The crude fiber (15.738%-17.714%) and crude protein (10.676%-12.019%). The conclusion of this study was that the addition of mix culture probiotics on anaerobic fermentation of coffee peel did not affect the crude fiber and crude protein content.
Senyawa Bioaktif KANDUNGAN SENYAWA BIOAKTIF KULIT JENGKOL (ARCHIDENDRON JIRINGA) DENGAN PERBEDAAN KONSENTRASI PELARUT ETANOL SEBAGAI POTENSI FEED ADDITIVE TERNAK RUMINANSIA: KANDUNGAN SENYAWA BIOAKTIF KULIT JENGKOL MENGGUNAKAN PERBEDAAN KONSENTRASI PELARUT ETANOL Purnama Ningrum, Ningrum; Hartati, Lilis; Suhendra, Danes; Hidayah, Nur; Rahayu, Tri Puji
Jurnal Peternakan Nusantara Vol. 10 No. 1 (2024)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/jpn.v10i1.10839

Abstract

This study was aimed to determine the content of bioactive compounds in jengkol peel (Archidendron Jiringa) with different concentrations of ethanol as a potential feed additive for ruminants. The material used in this study was 50g jengkol peel flour in each treatment. The research method used was an experimental trial using a Completely Randomized Design (RAL) with 5 treatments and 4 replications. The treatments used were P1 (100% aquadest), P2 (24% ethanol (75% aquadest + 25% ethanol)), P3 (48% ethanol (50% aquadest + 50% ethanol)), P4 (72% ethanol (25% aquadest + 75% ethanol)), and P5 (96% ethanol (100% ethanol)). The parameters observed in this study were tannin content, saponin, flavonoid, and total phenol. The data obtained were analyzed using Analysis of Variance (ANOVA), then if there were significantly differences, Duncan Multiple Range Test (DMRT) was carried out. The results of the study of jengkol peel extract using ethanol solvents with different concentrations gave significantly different results of tannin and saponin content (P<0.05), while the flavonoid content and total phenol were not significantly different (P>0.05). Based on the research conducted, it is concluded that jengkol peel extract has the potential as a feed additive for ruminants, as evidenced by the results of the tannin content of 8,22% and the saponin content of 13,47% with an ethanol concentration of 48%, but did not affect the flavonoid content and total phenol extracted from jengkol peel (Archidendron jiringa).
Bobot Karkas dan Persentase Organ Itik Magelang Jantan (Anas Platyrhynchos) yang Diberi Suplemen Inulin Pada Ransumnya Lilis Hartati; Galy Hardyta; Tri Puji Lestari; Rizki Nur Amin
Jurnal Nutrisi Ternak Tropis Vol. 7 No. 1 (2024): JNT Jurnal Nutrisi Ternak Tropis Maret
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jnt.2024.007.01.4

Abstract

Itik Magelang merupakan salah satu jenis unggas dwiguna yang mampu menghasilkan daging dan telur. Optimalisasi produktivitas itik dapat dilakukan dengan memberikan imbuhan pakan (feed aditif) berupa prebiotik. Penelitian ini bertujuan untuk mengkaji pengaruh penambahan inulin dalam pakan terhadap bobot karkas dan persentase organ dalam Itik Magelang jantan. Rancangan percobaan pada penelitian ini adalah Rancangan Acak Lengkap (RAL). Penelitian ini menggunakan itik Magelang jantan sebanyak 100 ekor. Penelitian ini terdiri dari 4 perlakuan dengan 5 ulangan. Perlakuannya adalah: P0 (pakan control) P1(pakan + Inulin 0,5%) P2 (pakan + Inulin 1%) P3 (Pakan + Inulin 1,5%) dengan jumlah itik ulangan adalah 5 ekor. Variabel yang diamati meliputi: bobot karkas, dan persentase organ dalam (hati, jantung, ampela, dan Limpa). Analisis data menggunakan analisis ragam (ANOVA) dan data yang berbeda nyata diuji lanjut Duncan's Multiple Range (DMRT). Hasil penelitian ini menunjukkan bahwa penambahan inulin pada pakan tidak berpengaruh nyata terhadap bobot karkas dan persentase organ dalam. Bobot karkas (899,35 ± 92,39) persentase jantung (0,87 ± 0,52%) persentase hati (2,70 ± 0,35%) persentase ampela (4,36 ± 0,47%) persentase limpa (0,11 ± 0,05%) yang masih ke dalam kondisi normal. Berdasarkan hasil penelitian dapat disimpulkan bahwa penambahan prebiotik inulin sebanyak 0, 0,5, 1, dan 1,5% dalam pakan itik Magelang jantan tidak berpengaruh nyata terhadap bobot karkas dan persentase organ dalam (jantung, hati, ampela, dan limpa).
The Effect of Azolla microphylla Substitution in Feed on Carcass Weight and Percentage of Male Magelang Ducks Ahmad, Misbah Aqwam Ahmad; Lilis Hartati; Mikael Sihite; Danes Suhendra; Tri Puji Rahayu
Jurnal Ilmu Dan Teknologi Peternakan Indonesia (JITPI) Indonesian Journal of Animal Science and Technology) Vol 10 No 1 (2024): Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI) Indonesian Journal of Ani
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jitpi.v10i1.195

Abstract

Produktivitas itik belum optimal akibat biaya pakan sumber protein yang mahal. Azolla microphylla dianggap sebagai gulma dan belum banyak dimanfaatkan. Penelitian ini bertujuan untuk mengetahui dan mengkaji mengkaji alternatif bahan pakan yang dapat digunakan untuk substitusi protein dalam ransum itik. Materi penelitian yang digunakan adalah 40 ekor itik magelang umur lima hari yang dikelompokkan dalam 4 perlakuan, meliputi: (P0) kontrol tanpa substitusi Azolla microphylla, (P1) substitusi 10% Azolla microphylla, (P2) substitusi 15% Azolla microphylla, dan (P3) substitusi 20% Azolla microphylla. Rancangan percobaan pada penelitian ini adalah Rancangan Acak Lengkap (RAL) nonfaktorial. Variabel yang diamati meliputi bobot karkas dan persentase karkas. Data dianalisis menggunakan analisis ragam (ANOVA) taraf nyata 95% dan perbedaan nyata dianalisis dengan uji lanjut Duncan’s Multiple Range Test (DMRT). Substitusi Azolla microphylla pada pakan berpengaruh pada bobot karkas dengan rata-rata yang diperoleh 729,9±90,9a sampai 912,9±14,6b gram/ekor, tetapi tidak berpengaruh terhadap persentase karkas dengan rata-rata 55,48±5,09 sampai 61,16±2,14. Kesimpulan dari penelitian ini adalah Penggunaan Azolla microphylla dengan proporsi hingga 20% masih dalam batas aman dan menghasilkan persentase karkas yang normal.
Model of the relationship between egg weight and the external quality of Pengging duck (Anas plathyrynchos) Hartati, Lilis; Sihite, Mikael; Bramantiar, Sambodo Ilham
Jurnal Ilmu Peternakan Terapan Vol 8 No 1 (2024): Jurnal Ilmu Peternakan Terapan
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/jipt.v8i1.5327

Abstract

This research was designed to analyze the relationship between egg weight and the external quality of Pengging duck eggs. The method used in the research was quantitative observational.  Samples of Pengging Duck eggs were taken from the Banyubiru Satker Center, Semarang Regency, totaling 250 eggs. The variables measured include egg weight, egg length and egg width, egg length and width circumference, egg volume, egg specific gravity, egg index and egg circumference index. The analysis used was regression analysis with egg weight as the dependent variable (Y) and external egg quality (egg length, etc.) as the independent variable (X). Relationship models obtained for egg length-egg weight (Y=13.872x-11.994), egg width-egg weight (Y=21.392x-31.395), egg circumference index-egg weight (Y=1.9624x+33.523), width circumference-egg weight (Y=3.8527x+8.4576), egg specific gravity-egg weight (Y=-8.931x+76.308), egg index-egg weight (Y=-8.166x+72.718), egg circumference index-egg weight (Y=-7.339x+72.69). It could be concluded that the length, width, length perimeter, width perimeter, and specific gravity of the eggs were found to be highly related to egg weight of Pengging Duck.
Kualitas Fisik Daging Itik Magelang Jantan (Anas platyrhynchos) Yang Diberi Suplemen Inulin Pada Pakan Hartati, Lilis; Hardyta, Galy; Rahayu, Tri Puji; Maulana, Aditya Iqbal
Jurnal Nutrisi Ternak Tropis Vol. 8 No. 1 (2025): JNT Jurnal Nutrisi Ternak Tropis Maret
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jnt.2025.008.01.3

Abstract

Penelitian ini bertujuan untuk mengkaji pengaruh penambahan inulin dalam ransum terhadap kualitas fisik daging Itik Magelang Jantan. Rancangan percobaan pada penelitian ini adalah Rancangan Acak Lengkap (RAL). Penelitian ini menggunakan itik Magelang jantan sebanyak 100 ekor. Penelitian ini terdiri dari 4 perlakuan dengan 5 ulangan. Perlakuannya adalah: P0 (pakan kontrol), P1(pakan + Inulin 0,5%), P2 (pakan + Inulin 1%), dan P3 (Pakan + Inulin 1,5%) dengan 5 ekor itik setiap ulangan. Variabel yang diamati meliputi: pH, daya ikat air, susut masak, dan kadar air. Data yang diperoleh dianalisa analisis ragam (ANOVA). Hasil penelitian menunjukkan penambahan inulin pada pakan tidak berpengaruh nyata terhadap pH, daya ikat air, susut masak, dan kadar air. Nilai rata-rata pH 6,10 ± 0,50, daya ikat air 81,25 ± 9,98%, susut masak 33,01 ± 3,61%, kadar air 65,02 ± 2,13%. Berdasarkan hasil penelitian dapat disimpulkan bahwa penambahan prebiotik inulin sebesar 0,5%,1% dan 1,5% tidak berpengaruh terhadap kualitas fisik daging itik (pH, daya ikat air, susut masak, kadar air).
NDF (Neutral Detergent Fiber) and ADF (Acid Detergent Fiber) In Vitro Digestibility of Fermented Complete Feed with Lemongrass Oil Distillation Waste Hartati, Lilis; Septian, Mohamad Haris; Afsari, Restiana Nur
JURNAL ILMIAH PETERNAKAN TERPADU Vol. 13 No. 3 (2025)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v13i3.p1013-1026

Abstract

This study aims to evaluate the potential of fermented lemongrass distillation waste as a complete feed for ruminant livestock by assessing in vitro digestibility of NDF and ADF. The study was designed using a Completely Randomized Design (CRD) with five treatments and four replications, including: P0 (60% Elephant Grass + 0% Lemongrass Distillation Waste + 40% Concentrate), P1 (40% Elephant Grass + 20% Lemongrass Distillation Waste + 40% Concentrate), P2 (30% Elephant Grass + 30% Lemongrass Distillation Waste + 40% Concentrate), P3 (20% Elephant Grass + 40% Lemongrass Distillation Waste + 40% Concentrate), and P4 (0% Elephant Grass + 60% Lemongrass Distillation Waste + 40% Concentrate). The observed parameters were in vitro NDF digestibility and ADF digestibility. Data were analyzed using analysis of variance (ANOVA). The differences in the use of fermented lemongrass distillation waste in complete feed did not affect (P>0.05) the in vitro digestibility of NDF and ADF, with an average NDF digestibility of 69.24 ± 10.42% and an average ADF digestibility of 36.97 ± 7.18%. The results of this study conclude that lemongrass distillation waste can be utilized as an alternative fiber source in formulating complete feed for small ruminant livestock.