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All Journal Klinikal Sains (Jurnal Analis Kesehatan) Quantum Wellness : Jurnal Ilmu Kesehatan Journal of Medical Laboratory in Infectious and Degenerative Diseases
Rian Anggia Destiawan
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Evaluasi Cemaran Bakteri Escherichia coli dan Coliform pada Air Minum Isi Ulang di Kecamatan Patrang, Jember Alisyah Pratiwi; Hartalina Mufidah; Rian Anggia Destiawan
Quantum Wellness : Jurnal Ilmu Kesehatan Vol. 2 No. 4 (2025): Desember : Quantum Wellness : Jurnal Ilmu Kesehatan
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62383/quwell.v2i4.2523

Abstract

Water plays a fundamental role in sustaining human life, particularly as a source of drinking water. Nonetheless, the consumption of water that does not comply with established health standards can result in illnesses such as diarrhea and vomiting. In the Patrang District, the use of refillable drinking water has gained popularity as an economical substitute for bottled water. Despite its convenience, there are growing concerns about the microbiological safety of refillable drinking water, especially regarding contamination by Coliform and Escherichia coli bacteria, which serve as key indicators of water hygiene and safety. The objective of this research was to determine the contamination levels of Coliform and Escherichia coli bacteria in refillable drinking water. The study utilized a quantitative research design with descriptive analysis and employed the Most Probable Number (MPN) method for testing. Data collection took place from May to June 2024, comprising five water samples labeled X, Y, Z, A, and B, obtained from refillable drinking water depots in Patrang District using a simple random sampling approach. The results indicated that out of the five samples tested, the sample labeled Z was positive for Coliform with an MPN value of 920 cfu/mL, while the sample labeled A was positive for both Coliform and Escherichia coli with an MPN value of 2,000 cfu/mL. From these findings, two samples (Z and A) were identified as contaminated, with the highest bacterial concentration found in sample A, which had an MPN value of 2,000 cfu/mL
UJI ANTIMIKROBA EKSTRAK DAUN CIPLUKAN (Physalis angulata L.) TERHADAP PERTUMBUHAN BAKTERI Streptococcus pyogenes Anas Fadli Wijaya; Rian Anggia Destiawan; Ayu Tri Agustin; Hartalina Mufidah; Mutia Hariani Nurjanah
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.5282

Abstract

Infectious diseases caused by microbes in Indonesia are mostly due to bacterial infections. One of the bacteria responsible is Streptococcus pyogenes, which causes Acute Respiratory Infections. Treatment of infections caused by S. pyogenes mostly involves the use of antibiotics. In some cases, antibiotics often lead to resistance, necessitating alternative solutions, such as antimicrobial compounds derived from natural sources like flavonoids, one of which can be found in the extract of ground cherry leaf (Physalis angulata L.). The aim of this study is to investigate the antibacterial effects of a 70% ethanol extract of ground cherry leaves on the growth of S. pyogenes. This research is an experimental laboratory study using a post-test control group design only. The determination of antimicrobial activity was carried out using the well diffusion method. The ground cherry leaf extract, diluted with 10% DMSO, was pipetted into the wells. The positive control used chloramphenicol, while the negative control used 10% DMSO. Based on the observations, an inhibition zone was formed in the positive control with an average of 26.18 ± 1.88 mm, treatment 1 with 100% ground cherry leaf extract 16.22 ± 1.33 mm, treatment 2 with 50% ciplukan leaf extract 15.09 ± 1.01 mm, and treatment 3 with 25% ground cherry leaf extract 12.85 ± 0.87 mm.
Effectiveness Of Starfruit (Averrhoa bilimbi L.) Extract Against Head Lice (Pediculus humanus capitis) Mortality Dini Athira; Rian Anggia Destiawan; Ahdiah Imroatul Muflihah
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 3 No. 1 (2025): Juni
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v3i1.42

Abstract

Background: Head lice (Pediculus humanus capitis) are parasites that suck human blood. The disease caused by head lice is called Pediculosis which still occurs in densely populated areas such as Islamic boarding schools. The compounds contained in bilimbung wuluh fruit such as saponins and flavonoids are believed to help kill head lice. Purpose: This study aims to determine the effectiveness of star fruit extract (Averrhoa bilimbi L.) on the mortality of head lice (Pediculus humanus capitis). Methods: The method in this research is experimental with a Post Only Control Group research design. This study used 384 head lice samples with each group of 16 head lice samples sprayed with star fruit extract with concentrations of 10%, 20%, 30% and 40%. As well as using the gold standard anti-head lice drug Peditox and negative control in the form of sterile distilled water. Results: The results of this study indicate that star fruit extract (Averrhoa bilimbi L.) is effective in killing head lice (Pediculus humanus capitis) at concentrations of 20%, 30%, 40% within 30 minutes. However, at a concentration of 10% it is not effective in killing head lice. The Kruskal Wallis test showed that there was an effect of starfruit extract on head lice mortality with a significant value of 0.001 p-value ≤0.05. In the Maan-Whitney U follow-up test, it was found that there were differences in the test groups with a significant value of ≤0.05. However, in the 10% extract test group and the negative control group, there was no significant difference in values ≥0.05. Conclusions: Star fruit extract (Averrhoa bilimbi L.) is the most effective for killing head lice (Pediculus humanus capitis) within 30 minutes, namely a concentration of 40%.
Co-Authors Agustin, Ayu Tri Ahdiah Imroatul Muflihah AHDIAH IMROATUL MUFLIHAH Alisa Alfiya Makki Alisyah Pratiwi Anas Fadli Wijaya Dini Athira Hartalina Mufidah Laila Camelia Nur Azizah Leny Yulia Widia Mutia Hariani Nurjanah Queen Nurul Sufi