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All Journal Jurnal Analis Kesehatan Klinikal Sains (Jurnal Analis Kesehatan) Quantum Wellness : Jurnal Ilmu Kesehatan Journal of Medical Laboratory in Infectious and Degenerative Diseases
Hartalina Mufidah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Evaluasi Cemaran Bakteri Escherichia coli dan Coliform pada Air Minum Isi Ulang di Kecamatan Patrang, Jember Alisyah Pratiwi; Hartalina Mufidah; Rian Anggia Destiawan
Quantum Wellness : Jurnal Ilmu Kesehatan Vol. 2 No. 4 (2025): Desember : Quantum Wellness : Jurnal Ilmu Kesehatan
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62383/quwell.v2i4.2523

Abstract

Water plays a fundamental role in sustaining human life, particularly as a source of drinking water. Nonetheless, the consumption of water that does not comply with established health standards can result in illnesses such as diarrhea and vomiting. In the Patrang District, the use of refillable drinking water has gained popularity as an economical substitute for bottled water. Despite its convenience, there are growing concerns about the microbiological safety of refillable drinking water, especially regarding contamination by Coliform and Escherichia coli bacteria, which serve as key indicators of water hygiene and safety. The objective of this research was to determine the contamination levels of Coliform and Escherichia coli bacteria in refillable drinking water. The study utilized a quantitative research design with descriptive analysis and employed the Most Probable Number (MPN) method for testing. Data collection took place from May to June 2024, comprising five water samples labeled X, Y, Z, A, and B, obtained from refillable drinking water depots in Patrang District using a simple random sampling approach. The results indicated that out of the five samples tested, the sample labeled Z was positive for Coliform with an MPN value of 920 cfu/mL, while the sample labeled A was positive for both Coliform and Escherichia coli with an MPN value of 2,000 cfu/mL. From these findings, two samples (Z and A) were identified as contaminated, with the highest bacterial concentration found in sample A, which had an MPN value of 2,000 cfu/mL
Analisis Pemantapan Mutu Internal Pada Pemeriksaan Trombosit Dan Leukosit Menggunakan Metode Sigma Metric Di Puskesmas X Muflihah, Ahdiah Imroatul; Muhammad Wildanun Ulum; Anas Fadli Wijaya; Hartalina Mufidah
Jurnal Analis Kesehatan Vol. 14 No. 2 (2025): JURNAL ANALIS KESEHATAN
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v14i2.5458

Abstract

Pemantapan mutu internal (PMI) di laboratorium krusial dilakukan untuk pengawasan dan pencegahan kesalahan hasil pemeriksaan, salah satu PMI dilakukan pada pemeriksaan trombosit dan leukosit menggunakan hematology analyzer untuk pencegahan kesalahan baca. Penelitian ini bertujuan untuk menganalisis hasil kontrol kualitas berapa akurasi, presisi, grafik Levey Jenning dengan aturan Westgard dan Sigma Metrik. Metode pada penelitian ini menggunakan metode deksriptif kuantitatif dengan menggunakan data sekunder pemeriksaan kontrol trombosit dan leukosit pada bulan Juli - Oktober 2024. Data kontrol pemeriksaan trombosit dan leukosit dianalisis berdasarkan aturan Westgard dan Sigma Metrik. Hasil pemantapan mutu internal pada penelitian ini menunjukkan bahwa keduanya memiliki tingkat akurasi yang baik karena berada dalam batas bias ±10%. Nilai presisi memenuhi kriteria karena tidak keluar dari nilai CV >5%. Evaluasi grafik Levey–Jennings berdasarkan aturan Westgard tidak menunjukkan pelanggaran aturan. Nilai Sigma Metrik untuk trombosit berada pada kategori sangat baik (>6), sedangkan nilai sigma metrik untuk leukosit berada pada kisaran >2 yang masih dapat dinilai layak untuk digunakan dalam pemeriksaan sampel.
UJI ANTIMIKROBA EKSTRAK DAUN CIPLUKAN (Physalis angulata L.) TERHADAP PERTUMBUHAN BAKTERI Streptococcus pyogenes Anas Fadli Wijaya; Rian Anggia Destiawan; Ayu Tri Agustin; Hartalina Mufidah; Mutia Hariani Nurjanah
Klinikal Sains : Jurnal Analis Kesehatan Vol. 12 No. 2 (2024): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v12i2.5282

Abstract

Infectious diseases caused by microbes in Indonesia are mostly due to bacterial infections. One of the bacteria responsible is Streptococcus pyogenes, which causes Acute Respiratory Infections. Treatment of infections caused by S. pyogenes mostly involves the use of antibiotics. In some cases, antibiotics often lead to resistance, necessitating alternative solutions, such as antimicrobial compounds derived from natural sources like flavonoids, one of which can be found in the extract of ground cherry leaf (Physalis angulata L.). The aim of this study is to investigate the antibacterial effects of a 70% ethanol extract of ground cherry leaves on the growth of S. pyogenes. This research is an experimental laboratory study using a post-test control group design only. The determination of antimicrobial activity was carried out using the well diffusion method. The ground cherry leaf extract, diluted with 10% DMSO, was pipetted into the wells. The positive control used chloramphenicol, while the negative control used 10% DMSO. Based on the observations, an inhibition zone was formed in the positive control with an average of 26.18 ± 1.88 mm, treatment 1 with 100% ground cherry leaf extract 16.22 ± 1.33 mm, treatment 2 with 50% ciplukan leaf extract 15.09 ± 1.01 mm, and treatment 3 with 25% ground cherry leaf extract 12.85 ± 0.87 mm.
Evaluation of Liver Function Through SGOT and SGPT Quantification in Rats Administered Polyethylene Terephthalate Microplastics Diana Nadhifah; Hartalina Mufidah; Ahdiah Imroatul Muflihah
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 3 No. 1 (2025): Juni
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v3i1.29

Abstract

Background: Microplastics, as emerging environmental pollutants, can accumulate in target organs such as the liver and induce hepatotoxicity through oxidative stress mechanisms. Purpose: To determine the effect of PET microplastic exposure at various doses on liver transaminase enzyme levels (SGOT and SGPT) in white rats. Methods: This experimental study used 25 male white rats (Rattus norvegicus), aged 8–10 weeks, divided into five groups (n=5 per group): control (distilled water), P1 (0.4 mg/day), P2 (0.6 mg/day), P3 (0.8 mg/day), and P4 (1.0 mg/day) of orally administered PET microplastics for 28 days. SGOT and SGPT levels were measured using spectrophotometry. Statistical analysis was performed using ANOVA followed by Tukey-HSD or Bonferroni post hoc tests (α=0.05). Results: The SGPT level in the control group (61.48 ± 7.50 U/L) significantly increased to 114.40 ± 7.71 U/L (P1), 132.22 ± 18.41 U/L (P2), 191.78 ± 16.42 U/L (P3), and 227.26 ± 12.23 U/L (P4). The SGOT level in the control group (78.64 ± 9.48 U/L) significantly increased to 188.46 ± 2.55 U/L (P1), 195.10 ± 5.75 U/L (P2), 238.90 ± 41.34 U/L (P3), and 310.74 ± 26.66 U/L (P4). Statistical analysis revealed significant differences among groups (p < 0.05). The higher the PET microplastic dose, the higher the SGOT and SGPT levels observed. Conclusions: ET microplastic exposure induces hepatotoxicity, as evidenced by dose-dependent increases in SGOT and SGPT levels.
Antibacterial Activity of Papaya (Carica papaya L.) Seed Ethanol Extract against Staphylococcus aureus Isolated from Diabetic Ulcers Diah Putri Aprilia; Hartalina Mufidah; Anas Fadli Wijaya
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 3 No. 1 (2025): Juni
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v3i1.30

Abstract

Background: Staphylococcus aureus is a bacterium that causes infections and is pathogenic to humans. Papaya (Carica papaya L.) seed extract contains various bioactive compounds, including tocopherols, terpenoids, flavonoids, alkaloids such as carpaine, and enzymes such as papain and lysozyme, which are known for their potential antimicrobial effects. Purpose: This study aimed to determine the antibacterial activity of papaya seed ethanol extract against S. aureus isolated from the foot ulcers of diabetic patients. Methods: An experimental quantitative study was conducted to evaluate the antibacterial activity of papaya seed ethanol extract at concentrations of 40%, 60%, and 80%. The extracts were prepared using the Ultrasonic-Assisted Extraction (UAE) method. Antibacterial activity was assessed by measuring the inhibition zones formed against S. aureus. Data were analyzed using a one-way ANOVA followed by a post hoc test to determine statistical significance. Results: The papaya seed extract exhibited concentration-dependent antibacterial activity against S. aureus. The mean inhibition zones were 0.00 mm at 40%, 15.07 mm at 60%, and 18.83 mm at 80%, whereas the positive control produced an inhibition zone of 46.36 mm. Conclusions: Papaya seed ethanol extract demonstrated antibacterial activity against S. aureus isolated from diabetic foot ulcers, with the strongest inhibition observed at the 80% concentration.
Effects of Various Mung Bean Decoction Doses on Hematological Profile in Anemic Wistar Rats Nabila Dwi Ayu Pratiwi; Hartalina Mufidah; Ahdiah Imroatul Muflihah
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 3 No. 1 (2025): Juni
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v3i1.32

Abstract

Background: Anemia remains a significant global public health problem, with iron deficiency being the primary cause. Mung beans (Vigna radiata L.) are nutritionally rich plants containing iron and glycine, which are essential for hemoglobin synthesis and red blood cell formation Purpose: This study aimed to evaluate the effects of various doses of mung bean decoction on hematological profile improvement in anemic Wistar rats induced with sodium nitrite (NaNO₂). Methods: An experimental study was conducted from June to August 2024 involving 25 male Wistar rats divided into five groups: healthy control (aquadest), negative control (NaNO₂), and three treatment groups receiving NaNO₂ followed by mung bean decoction at doses of 0.2 ml, 0.4 ml, and 0.8 ml respectively. Blood samples were collected before and after treatment for hemoglobin level assessment. Data were analyzed using paired sample t-test and One-way ANOVA with post-hoc Tukey tests. Results: The One-way ANOVA test showed highly significant differences in hemoglobin level changes between groups (p < 0.0001). The 0.2 ml dose group exhibited the highest hemoglobin increase from 8.12 g/dl to 17.70 g/dl (p = 0.0002), followed by the 0.4 ml dose group with an increase from 7.32 g/dl to 16.08 g/dl (p < 0.0001), and the 0.8 ml dose group with an increase from 6.40 g/dl to 14.16 g/dl (p = 0.0005). Conclusions: Mung bean decoction effectively improved hemoglobin levels in anemic Wistar rats, with the 0.2 ml dose demonstrating the most pronounced therapeutic effect.
Co-Authors Agustin, Ayu Tri Ahdiah Imroatul Muflihah Alisyah Pratiwi Anas Fadli Wijaya Diah Putri Aprilia Diana Nadhifah Muflihah, Ahdiah Imroatul Muhammad Wildanun Ulum Mutia Hariani Nurjanah nabila dwi ayu pratiwi Rian Anggia Destiawan