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All Journal BERNAS: Jurnal Pengabdian Kepada Masyarakat Journal of Medical Laboratory in Infectious and Degenerative Diseases
Ahdiah Imroatul Muflihah
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Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Dentistry Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Promosi Kesehatan Pecegahan Infeksi Salmonella Typhi Untuk Mendukung Program Kesehatan Masyarakat Rian Anggia Destiawan; Sholihatil Hidayati; Dhina Ayu Susanti; Ahdiah Imroatul Muflihah; Siti Huzaimah; Tiara Wahyu Norbaity
BERNAS: Jurnal Pengabdian Kepada Masyarakat Vol. 5 No. 1 (2024)
Publisher : Universitas Majalengka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31949/jb.v5i1.6989

Abstract

Salmonella Merupakan bakteri gram negatif berbentuk batang, dan memiliki flagela. Nama Penyakit yang disebabkan oleh bakteri Salmonella adalah salmonellosis yang menyebabkan demam tifoid. Salmonellosis dapat ditularkan melalui makanan dan minuman. Tujuan dari pengabdian masyarakat adalah untuk menggalakkan kesehatan pencegahan infeksi salmonella typhi di masyarakat untuk mengurangi penyakit yang disebabkan oleh bakteri Salmonella typhi. Metode pengabdian masyarakat ini adalah dengan memberikan materi kepada masyarakat terkait penyakit tipus yang disebabkan oleh infeksi Salmonella typhi dan melihat masukan dari masyarakat. Hasil yang diperoleh dari pengabdian masyarakat dukungan masyarakat untuk demam tifoid yaitu dengan cara menjaga kebersihan lingkungan, Menjaga imunitas, menjaga kebersihan makanan, dan mencuci tangan sebelum makan. Kesimpulan dari pengabdian masyarakat ini adalah adanya pemahaman masyarakat baik dalam pencegahan penyakit tifoid yang disebabkan oleh bakteri Salmonella typhi.
Evaluation of Liver Function Through SGOT and SGPT Quantification in Rats Administered Polyethylene Terephthalate Microplastics Diana Nadhifah; Hartalina Mufidah; Ahdiah Imroatul Muflihah
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 3 No. 1 (2025): Juni
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v3i1.29

Abstract

Background: Microplastics, as emerging environmental pollutants, can accumulate in target organs such as the liver and induce hepatotoxicity through oxidative stress mechanisms. Purpose: To determine the effect of PET microplastic exposure at various doses on liver transaminase enzyme levels (SGOT and SGPT) in white rats. Methods: This experimental study used 25 male white rats (Rattus norvegicus), aged 8–10 weeks, divided into five groups (n=5 per group): control (distilled water), P1 (0.4 mg/day), P2 (0.6 mg/day), P3 (0.8 mg/day), and P4 (1.0 mg/day) of orally administered PET microplastics for 28 days. SGOT and SGPT levels were measured using spectrophotometry. Statistical analysis was performed using ANOVA followed by Tukey-HSD or Bonferroni post hoc tests (α=0.05). Results: The SGPT level in the control group (61.48 ± 7.50 U/L) significantly increased to 114.40 ± 7.71 U/L (P1), 132.22 ± 18.41 U/L (P2), 191.78 ± 16.42 U/L (P3), and 227.26 ± 12.23 U/L (P4). The SGOT level in the control group (78.64 ± 9.48 U/L) significantly increased to 188.46 ± 2.55 U/L (P1), 195.10 ± 5.75 U/L (P2), 238.90 ± 41.34 U/L (P3), and 310.74 ± 26.66 U/L (P4). Statistical analysis revealed significant differences among groups (p < 0.05). The higher the PET microplastic dose, the higher the SGOT and SGPT levels observed. Conclusions: ET microplastic exposure induces hepatotoxicity, as evidenced by dose-dependent increases in SGOT and SGPT levels.
Effects of Various Mung Bean Decoction Doses on Hematological Profile in Anemic Wistar Rats Nabila Dwi Ayu Pratiwi; Hartalina Mufidah; Ahdiah Imroatul Muflihah
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 3 No. 1 (2025): Juni
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v3i1.32

Abstract

Background: Anemia remains a significant global public health problem, with iron deficiency being the primary cause. Mung beans (Vigna radiata L.) are nutritionally rich plants containing iron and glycine, which are essential for hemoglobin synthesis and red blood cell formation Purpose: This study aimed to evaluate the effects of various doses of mung bean decoction on hematological profile improvement in anemic Wistar rats induced with sodium nitrite (NaNO₂). Methods: An experimental study was conducted from June to August 2024 involving 25 male Wistar rats divided into five groups: healthy control (aquadest), negative control (NaNO₂), and three treatment groups receiving NaNO₂ followed by mung bean decoction at doses of 0.2 ml, 0.4 ml, and 0.8 ml respectively. Blood samples were collected before and after treatment for hemoglobin level assessment. Data were analyzed using paired sample t-test and One-way ANOVA with post-hoc Tukey tests. Results: The One-way ANOVA test showed highly significant differences in hemoglobin level changes between groups (p < 0.0001). The 0.2 ml dose group exhibited the highest hemoglobin increase from 8.12 g/dl to 17.70 g/dl (p = 0.0002), followed by the 0.4 ml dose group with an increase from 7.32 g/dl to 16.08 g/dl (p < 0.0001), and the 0.8 ml dose group with an increase from 6.40 g/dl to 14.16 g/dl (p = 0.0005). Conclusions: Mung bean decoction effectively improved hemoglobin levels in anemic Wistar rats, with the 0.2 ml dose demonstrating the most pronounced therapeutic effect.
The Effect of Papaya Leaf Extract (Carica papaya L.) on Erythrocyte Values in Anemia-Model Rats Fajar Dwi Susanto; Leny Yulia Widia Sari; Ahdiah Imroatul Muflihah; Anas Fadli Wijaya; Ayu Tri Agustin
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 3 No. 2 (2025): Desember
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v3i2.34

Abstract

Background: Anemia remains a significant public health challenge with a global prevalence of 40-88%. Papaya leaf extract (Carica papaya L.) contains bioactive compounds such as flavonoids, vitamin C, and vitamin E that potentially enhance erythrocyte production. Purpose: This study aimed to determine the effect of papaya leaf extract on erythrocyte values in anemia-model rats (Rattus novergicus). Methods: An experimental laboratory study was conducted from April to June 2025 using 25 rats divided into 5 groups: positive control (sodium nitrite), negative control (aquadest), and three treatment groups receiving papaya leaf extract at doses of 100, 200, and 400 mg/kg BW. Anemia was induced using sodium nitrite at 50 mg/kg BW via intraperitoneal injection. Erythrocyte measurements were performed using a hematology analyzer. Data were analyzed using Shapiro-Wilk normality test, Levene's homogeneity test, one-way ANOVA followed by Tukey HSD (α=0.05). Results: Treatment group P3 (dose 400 mg/kg BW) demonstrated the highest erythrocyte value of 12.066±0.84 (10⁶/µL), followed by P1 (11.576±0.71), P2 (11.496±1.19), negative control (10.728±1.01), and positive control (6.220±1.96). ANOVA test revealed significant differences between groups (p<0.001). Conclusions: Papaya leaf extract increases erythrocyte values in anemia-model rats, with 400 mg/kg BW being the most effective dose.
Effectiveness Of Starfruit (Averrhoa bilimbi L.) Extract Against Head Lice (Pediculus humanus capitis) Mortality Dini Athira; Rian Anggia Destiawan; Ahdiah Imroatul Muflihah
Journal of Medical Laboratory in Infectious and Degenerative Diseases Vol. 3 No. 1 (2025): Juni
Publisher : LPPM Universitas dr. Soebandi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36858/jmid.v3i1.42

Abstract

Background: Head lice (Pediculus humanus capitis) are parasites that suck human blood. The disease caused by head lice is called Pediculosis which still occurs in densely populated areas such as Islamic boarding schools. The compounds contained in bilimbung wuluh fruit such as saponins and flavonoids are believed to help kill head lice. Purpose: This study aims to determine the effectiveness of star fruit extract (Averrhoa bilimbi L.) on the mortality of head lice (Pediculus humanus capitis). Methods: The method in this research is experimental with a Post Only Control Group research design. This study used 384 head lice samples with each group of 16 head lice samples sprayed with star fruit extract with concentrations of 10%, 20%, 30% and 40%. As well as using the gold standard anti-head lice drug Peditox and negative control in the form of sterile distilled water. Results: The results of this study indicate that star fruit extract (Averrhoa bilimbi L.) is effective in killing head lice (Pediculus humanus capitis) at concentrations of 20%, 30%, 40% within 30 minutes. However, at a concentration of 10% it is not effective in killing head lice. The Kruskal Wallis test showed that there was an effect of starfruit extract on head lice mortality with a significant value of 0.001 p-value ≤0.05. In the Maan-Whitney U follow-up test, it was found that there were differences in the test groups with a significant value of ≤0.05. However, in the 10% extract test group and the negative control group, there was no significant difference in values ≥0.05. Conclusions: Star fruit extract (Averrhoa bilimbi L.) is the most effective for killing head lice (Pediculus humanus capitis) within 30 minutes, namely a concentration of 40%.
Co-Authors Agustin, Ayu Tri Anas Fadli Wijaya Dhina Ayu Susanti Diana Nadhifah Dini Athira Fajar Dwi Susanto Hartalina Mufidah Leny Yulia Widia Sari nabila dwi ayu pratiwi RIAN ANGGIA DESTIAWAN Rian Anggia Destiawan Sholihatil Hidayati Siti Huzaimah Tiara Wahyu Norbaity