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Dewi Ranggaini
Department Of Physiology, Division Of Oral Biology, Faculty Of Dentistry, Universitas Trisakti, Jl. Kyai Tapa No. 260, Jakarta 11440
Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Aktivitas antioksidan ekstrak etanol rimpang curcuma xanthorrhiza Roxb. dan asam askorbat (Dengan metode DPPH, FRAP, dan H2O2) Winnie Susanto, Shannon; Dewi Ranggaini, Monica
Jurnal Kedokteran Gigi Terpadu Vol. 4 No. 1 (2022): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jkgt.v4i1.14276

Abstract

Background: C. xanthorrhiza Roxb. contains chemical compounds that beneficial as antioxidant. Extraction using ethanol 70% is required to obtain these compounds. The antioxidant activity is assessed by using DPPH, FRAP, and H2O2. Parameter to interpret the value is IC50 and the value is compared to absorbic acid. Purpose: To find out the comparison of antioxidant activity between C. xanthorrhiza Roxb. extract in ethanol 70% solvent and absorbic acid using DPPH, FRAP, and H2O2 methods. Method: Using laboratory research method. Simplicia of C. xanthorrhiza Roxb. was extracted using maceration method in 70% ethanol. The antioxidant activity was tested in the sample and the absorbic acid. The samples were incubated and measured at  = 520 nm for DPPH,  = 593 nm for FRAP, and  = 510 nm for H2O2. Result: The IC50 value of C. xanthorrhiza Roxb. extract was 194,74 g/mL DPPH, 49.69 g/mL FRAP, and 166,75 g/mL H2O2 , and for absorbic acid was 0,29 g/mL DPPH, 2,78 g/mL FRAP, and 0,55 g/mL H2O2. Conclusion: FRAP method is proven to be the strongest method for measuring antioxidant activity in C. xanthorrhiza Roxb. among the other methods.
Aktivitas antioksidan ekstrak etanol rimpang curcuma xanthorrhiza roxb. Dan asam askorbat (Dengan Metode DPPH, ABTS, Dan NO) Amanda Suwardi, Olivia; Dewi Ranggaini, Monica
Jurnal Kedokteran Gigi Terpadu Vol. 4 No. 1 (2022): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jkgt.v4i1.14475

Abstract

Background: Antioxidants are very important to prevent degenerative diseases. Natural ingredients used as natural antioxidants are C. xanthorrhiza Roxb. Purpose: To compare antioxidant activity between ethanol extract C. xanthorrhiza Roxb. and ascorbic acid with DPPH, ABTS, and NO methods. Methods: Experimental laboratory research. C. xanthorrhiza Roxb. was extracted by maceration method. In the DPPH, ABTS, and NO methods, C. xanthorrhiza Roxb. and ascorbic acid were incubated. Then the absorbance was measured at a wavelength 517 nm for the DPPH method, 745 nm for the ABTS method, and 516 nm for NO method. Results: In DPPH method, IC50 of ethanol extract C. xanthorrhiza Roxb. was 194.74 g/mL and 0.29 g/mL for ascorbic acid, in ABTS method IC50 of ethanol extract C. xanthorrhiza Roxb. was 80.04 g/mL and 2.49 g/mL for ascorbic acid, in NO method, IC50 of ethanol extract C. xanthorrhiza Roxb. was 164.11 g/mL and 0.54 g/mL for ascorbic acid. Conclusion: In DPPH and NO methods, C. xanthorrhiza Roxb. has weak antioxidant activity and in ABTS method has strong antioxidant activity. In DPPH, ABTS, and NO methods, ascorbic acid has very strong antioxidant activity.
Aktivitas Antioksidan dengan Metode DPPH dan ABTS Terhadap Ekstrak Etanol Daun Amaranthus hybridus L. Dewi Ranggaini, Monica; Halim, Johni; Aurelia Tjoe, Michelle
Jurnal Kedokteran Gigi Terpadu Vol. 6 No. 1 (2024): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jkgt.v6i1.20891

Abstract

Background: Cancer, a degenerative disease, arises from free radicals in the body, which can be counteracted by antioxidants. Amaranthus hybridus L., a vegetable rich in antioxidant compounds like flavonoids, requires extraction to harness these compounds. This extraction uses the maceration technique with 70% ethanol. Antioxidant activity is tested using methods like DPPH and ABTS assays, with the IC50 value indicating effectiveness. Purpose: To determine the difference in antioxidant activity of A. hybridus L. leaves ethanol extract with DPPH and ABTS methods. Method: Laboratory experimental research. Simplicia leaves of A. hybridus L. in 70% ethanol solvent were extracted using maseration method. The antioxidant activity was tested in the sample and the ascorbic acid using DPPH and ABTS methods at wavelenghts 517 nm and 745 nm. Sample absorbance was measured using microplate reader. Result: IC50 values of A. hybridus L. leaves ethanol extract with DPPH and ABTS methods were 54,99 µg/mL and 71,45 µg/mL, for ascorbic acid using DPPH and ABTS methods were 0,62 µg/mL and 3.13 µg/mL. Conclusion: The antioxidant activity of A. hybridus L. leaves ethanol extract with DPPH method has stronger antioxidant than ABTS method.
Inositol Hexakisphosphate (InsP₆) Induces Apoptosis via Caspase-Dependent Pathways: Molecular Docking Insights Ferry Sandra; Dewi Ranggaini; Johni Halim; Alfred Pakpahan; Visi Endah Pratitis; Kyung Hoon Lee
The Indonesian Biomedical Journal Vol 17, No 5 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i5.3810

Abstract

BACKGROUND: Inositol hexakisphosphate (InsP₆) exhibits anticancer activity, especially by inducing intrinsic and extrinsic apoptotic pathways. However, there is still no molecular docking evidence that directly examines InsP₆ interactions with either upstream or downstream apoptotic regulators. Therefore, the current study was conducted to investigate the molecular docking of InsP₆ to caspases as upstream/downstream apoptotic regulators.METHODS: Ligands including InsP₆, InsP₅, InsP₄, histone deacetylase inhibitor, and caspase inhibitors were retrieved from PubChem, while target proteins (histone, caspase-8, caspase-2, and caspase-3) were obtained from the Protein Data Bank. Ligand toxicity was predicted using ProTox-3.0, and physicochemical properties were analyzed with SwissADME. Ligand structures were energy-minimized using PyRx with the Universal Force Field, while proteins were prepared by removing water molecules and non-essential heteroatoms in BIOVIA Discovery Studio. Molecular docking was conducted using CB-Dock 2.0, with binding poses selected based on the lowest Vina score, and ligand–protein interactions were visualized in Discovery Studio.RESULTS: Molecular docking results showed that InsP₆ bound strongly to histone, caspase-8, caspase-2, and caspase-3 with affinities comparable to reference inhibitors, forming multiple hydrogen bonds with key active-site residues. InsP₆, InsP₅, and InsP₄ exhibited several similar binding sites to caspase-3, with only minor differences in binding affinity.CONCLUSION: InsP₆ shows strong binding to histone, caspase-8, caspase-2, and caspase-3 based on in silico results, supporting its role in inducing both extrinsic and intrinsic apoptotic pathways. Taken together, InsP₆ could be a potential inducer of apoptosis in cancer cells.KEYWORDS: cancer, apoptosis, InsP₆, InsP₅, InsP₄, caspase, in silico, molecular docking
Co-Authors Alfred Pakpahan Amanda Suwardi, Olivia Ariyani, Annisaa Putri Aurelia Tjoe, Michelle Bernard Ongki Iskandar Didi Nugroho Santosa Ferry Sandra Fika Alifiana Halim, Johni Himawan Halim Holie Fransiski Intan Paramitha Kumaladevi Johni Halim Johni Halim Johni Halim Joko Kusnoto Kyung Hoon Lee Laifa Annisa Hendarmin Maureen A. Wongso Melanie Sadono Djamil Nurrani Mustika Dewi Olivia Amanda Suwardi Richard Tridarmawan Richentya Feiby Salim Shannon Winnie Susanto Visi Endah Pratitis Winnie Susanto, Shannon Wita Anggraini Wiwiek Poedjiastoeti, Wiwiek Yayuk Yuliarsi