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Dewi Ranggaini
Department Of Physiology, Division Of Oral Biology, Faculty Of Dentistry, Universitas Trisakti, Jl. Kyai Tapa No. 260, Jakarta 11440
Biochemistry, Genetics & Molecular Biology Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Aktivitas Antioksidan dengan Metode DPPH dan ABTS Terhadap Ekstrak Etanol Daun Amaranthus hybridus L. Dewi Ranggaini, Monica; Halim, Johni; Aurelia Tjoe, Michelle
Jurnal Kedokteran Gigi Terpadu Vol. 6 No. 1 (2024): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jkgt.v6i1.20891

Abstract

Background: Cancer, a degenerative disease, arises from free radicals in the body, which can be counteracted by antioxidants. Amaranthus hybridus L., a vegetable rich in antioxidant compounds like flavonoids, requires extraction to harness these compounds. This extraction uses the maceration technique with 70% ethanol. Antioxidant activity is tested using methods like DPPH and ABTS assays, with the IC50 value indicating effectiveness. Purpose: To determine the difference in antioxidant activity of A. hybridus L. leaves ethanol extract with DPPH and ABTS methods. Method: Laboratory experimental research. Simplicia leaves of A. hybridus L. in 70% ethanol solvent were extracted using maseration method. The antioxidant activity was tested in the sample and the ascorbic acid using DPPH and ABTS methods at wavelenghts 517 nm and 745 nm. Sample absorbance was measured using microplate reader. Result: IC50 values of A. hybridus L. leaves ethanol extract with DPPH and ABTS methods were 54,99 µg/mL and 71,45 µg/mL, for ascorbic acid using DPPH and ABTS methods were 0,62 µg/mL and 3.13 µg/mL. Conclusion: The antioxidant activity of A. hybridus L. leaves ethanol extract with DPPH method has stronger antioxidant than ABTS method.
Aktivitas antioksidan ekstrak etanol rimpang curcuma xanthorrhiza Roxb. dan asam askorbat (Dengan metode DPPH, FRAP, dan H2O2) Winnie Susanto, Shannon; Dewi Ranggaini, Monica
Jurnal Kedokteran Gigi Terpadu Vol. 4 No. 1 (2022): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jkgt.v4i1.14276

Abstract

Background: C. xanthorrhiza Roxb. contains chemical compounds that beneficial as antioxidant. Extraction using ethanol 70% is required to obtain these compounds. The antioxidant activity is assessed by using DPPH, FRAP, and H2O2. Parameter to interpret the value is IC50 and the value is compared to absorbic acid. Purpose: To find out the comparison of antioxidant activity between C. xanthorrhiza Roxb. extract in ethanol 70% solvent and absorbic acid using DPPH, FRAP, and H2O2 methods. Method: Using laboratory research method. Simplicia of C. xanthorrhiza Roxb. was extracted using maceration method in 70% ethanol. The antioxidant activity was tested in the sample and the absorbic acid. The samples were incubated and measured at  = 520 nm for DPPH,  = 593 nm for FRAP, and  = 510 nm for H2O2. Result: The IC50 value of C. xanthorrhiza Roxb. extract was 194,74 g/mL DPPH, 49.69 g/mL FRAP, and 166,75 g/mL H2O2 , and for absorbic acid was 0,29 g/mL DPPH, 2,78 g/mL FRAP, and 0,55 g/mL H2O2. Conclusion: FRAP method is proven to be the strongest method for measuring antioxidant activity in C. xanthorrhiza Roxb. among the other methods.
Aktivitas antioksidan ekstrak etanol rimpang curcuma xanthorrhiza roxb. Dan asam askorbat (Dengan Metode DPPH, ABTS, Dan NO) Amanda Suwardi, Olivia; Dewi Ranggaini, Monica
Jurnal Kedokteran Gigi Terpadu Vol. 4 No. 1 (2022): Jurnal Kedokteran Gigi Terpadu
Publisher : Fakultas Kedokteran Gigi Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/jkgt.v4i1.14475

Abstract

Background: Antioxidants are very important to prevent degenerative diseases. Natural ingredients used as natural antioxidants are C. xanthorrhiza Roxb. Purpose: To compare antioxidant activity between ethanol extract C. xanthorrhiza Roxb. and ascorbic acid with DPPH, ABTS, and NO methods. Methods: Experimental laboratory research. C. xanthorrhiza Roxb. was extracted by maceration method. In the DPPH, ABTS, and NO methods, C. xanthorrhiza Roxb. and ascorbic acid were incubated. Then the absorbance was measured at a wavelength 517 nm for the DPPH method, 745 nm for the ABTS method, and 516 nm for NO method. Results: In DPPH method, IC50 of ethanol extract C. xanthorrhiza Roxb. was 194.74 g/mL and 0.29 g/mL for ascorbic acid, in ABTS method IC50 of ethanol extract C. xanthorrhiza Roxb. was 80.04 g/mL and 2.49 g/mL for ascorbic acid, in NO method, IC50 of ethanol extract C. xanthorrhiza Roxb. was 164.11 g/mL and 0.54 g/mL for ascorbic acid. Conclusion: In DPPH and NO methods, C. xanthorrhiza Roxb. has weak antioxidant activity and in ABTS method has strong antioxidant activity. In DPPH, ABTS, and NO methods, ascorbic acid has very strong antioxidant activity.
Co-Authors Amanda Suwardi, Olivia Ariyani, Annisaa Putri Aurelia Tjoe, Michelle Bernard Ongki Iskandar Didi Nugroho Santosa Ferry Sandra Fika Alifiana Halim, Johni Himawan Halim Holie Fransiski Intan Paramitha Kumaladevi Johni Halim Johni Halim Joko Kusnoto Laifa Annisa Hendarmin Maureen A. Wongso Melanie Sadono Djamil Nurrani Mustika Dewi Olivia Amanda Suwardi Richard Tridarmawan Richentya Feiby Salim Shannon Winnie Susanto Winnie Susanto, Shannon Wita Anggraini Wiwiek Poedjiastoeti, Wiwiek Yayuk Yuliarsi