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All Journal Biology, Medicine, & Natural Product Chemistry The Indonesian Biomedical Journal Acta Biochimica Indonesiana SRIWIJAYA JOURNAL OF MEDICINE Makara Journal of Health Research
Wahyunia Likhayati Septiana
Histology Department, Faculty Of Medicine, Gunadarma University, Depok, Indonesia
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Comparison of the effectiveness of physical training and extract of soursop leaf to histopathology of abdominal aorta foam cells in hipercolesterolemia-diabetes rat Tika Hamidah Apriliana Nai; Retno Yulianti; Wahyunia Likhayati Septiana; Yuni Setyaningsih
Acta Biochimica Indonesiana Vol. 3 No. 1 (2020): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v3i1.48

Abstract

Background: Diabetes mellitus has become a public health problem globally. Type 2 diabetes mellitus, caused by lifestyle and high lipid diet, is the most common form. Hypercholesterolemia-diabetes is able to create endothelial injury that results in formation of atherosclerosis, starting with formation of foam cells. Objective: This research aimed to see the effectiveness of physical training and soursop (Annona muricata) leaves extract in lowering the amount of foam cells. Methods: This research was an experimental study which used the post test-only control group design with 35 Wistar strain rats which were randomly divided into 7 groups, each consisting of 5 rats. K1 was given standard diet, K2 was given atherogenic diet and alloxan, while K3, K4, K5, K6, and K7 were given atherogenic diet, alloxan, and different treatments based on designed group. K3 was given simvastatin, K4 was given metformin, K5 was given physical training, K6 was given soursop leaves extract (SLE), and K7 was given both physical training and SLE. The rats were euthanized after three weeks and aorta was taken to be made into histology slides for foam cells observation. Results: The result of Kruskal-Wallis and Mann-Whitney Post-Hoc Test showed significant difference (p<0,05) between K2 and K5, K6, K7 while it no significant difference (p>0,05) between K3, K4 and K5, K6, K7. Conclusion: Physical training, SLE, and the combination of both have a role in lowering formation of foam cells in atherosclerosis.
CD34+ UCB stem cells attenuate TGF-β signaling and inhibit liver fibrosis: A new avenue for liver cirrhosis-carcinogenesis prevention Septiana, Wahyunia Likhayati; Antarianto, Radiana Dhewayani; Louisa, Melva; Jusuf, Ahmad Aulia; Barasila, Atikah Chalida; Pawitan, Jeanne Adiwinata; Fasha, Iqbal
Makara Journal of Health Research Vol. 24, No. 2
Publisher : UI Scholars Hub

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Abstract

Background: The liver microenvironment plays a key role in liver fibrosis and carcinogenesis. This study aimed to fill the gap in knowledge on the interaction between hepatic stellate cells and endothelial progenitor cells with biomarkers of liver fibrosis and/or carcinogenesis, including Col1A1, TGF-β, and tenascin-C. Methods: CD34+ stem cells were isolated from umbilical-cord-blood mononuclear cells. 2D and 3D co-culture of CD34+ UCB SCs and LX2 was performed. The cells were incubated in a CO2 incubator for three days. Morphological observation, qRT-PCR of TGF-β1 and COL1A1, and immunocytochemistry of tenascin-C were performed. Results: CD34+ UCB SCs were viable in the 2D and 3D co-culture for 24 h. 3D co-culture of CD34+ UCB SCs and LX2 inhibited in vitro liver fibrosis by lowering Col 1A1 expression as compared to control. We observed lower TGF-β expression in 3D co-culture on days 1 and 2 followed by higher expression of TGF-β on day 3. 2D co-culture of CD34+ UCB SCs and LX2 showed a different level of COL1A1 and TGF- β expression compared with 3D co-culture. Spheroids from 2D co-culture of CD34+ UCB SCs and LX-2 showed immunoreactivity against tenascin-C. Conclusion: Interaction between LX-2 and CD34+ UCB SCs in 3D co-culture inhibits in vitro liver fibrosis. The viability of CD34+ UCB SCs is essential for attenuation of TGF-β signaling in LX-2.
Correlation of Face Washing, Mask Use, and Acne Incidence During COVID-19 Among Gunadarma University Students Warni Zulhija Putri; Fifinela Raissa; Wahyunia Septiana; Endang Rahmawati
Sriwijaya Journal of Medicine Vol. 7 No. 2 (2024): Vol 7, No 2, 2024
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/sjm.v7i2.239

Abstract

Prevention of COVID-19, in addition to washing hands and keeping distance, can be achieved by using masks regularly. The use of masks in the long term can cause acne vulgaris. Acne vulgaris is a chronic inflammatory condition in the pilosebaceous follicle. Acne vulgaris often occurs in adolescents and young adults. Housekeeping face aims to reduce bacteria, dirt, or microorganisms from the skin's surface by reducing sebum and impurities without removing the lipid barrier in the face. This study aims to understand the relationship between the habit of washing the face and using masks during the COVID-19 pandemic and the incidence of acne vulgaris in students at Gunadarma University, Depok. This type of research is an analytic observational cross-sectional design; the primary data is collected through questionnaires. The sample of this research is the students of the faculty of medicine at Gunadarma University. The sample size is as many as 64, and the sampling technique is simple random sampling. The data were analyzed using the SPSS program and tested using a multivariate logistic regression test. Statistical tests between the facial washing habits and acne vulgaris show a p-value = 0.948. This study shows no relationship between facial washing habits and acne vulgaris. The statistical test results between mask use and acne vulgaris show a p-value = 0.017. This study shows the presence of meaningful relationships between the use of the mask and acne vulgaris (OR = 3.992). The statistical test results of the type of mask with acne vulgaris p value> 0.05. This study shows no relationship between the kind of mask and the incidence of acne vulgaris. This study shows no relationship between facial washing habits and acne vulgaris. Still, there is a relationship between the use of masks and acne vulgaris, and there is no relationship between the type of mask and the incidence of acne vulgaris.
N-Acetylcysteine Prevents Sleep Deprivation-induced Memory Deficit in Juvenile Rats Through the Suppression of BDNF, Cortisol, Acetylcholine Levels, and Inflammatory Cytokines Expressions Rosyidah, Citra; Arozal, Wawaimuli; Lee, Hee Jae; Tjandrawinata, Raymond Rubianto; Septiana, Wahyunia Likhayati
The Indonesian Biomedical Journal Vol 17, No 2 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i2.3445

Abstract

BACKGROUND: Sleep deprivation (SD) affects 20–30% of children and is known to impair cognitive functions, particularly memory. Despite its impact, there is currently no standardized treatment. Evidence from both adult animal and human suggests that N-acetylcysteine (NAC) possesses neuroprotective properties. This study was conducted to evaluate the effects of NAC on memory deficits induced by SD in juvenile rats.METHODS: Juvenile Sprague-Dawley rats were subjected to SD using the modified multiple platform method. NAC was administered intraperitoneally at doses of 100 mg/kgBW or 500 mg/kgBW. Y-maze and novel object recognition (NOR) tests were used for neurobehavioral assessment. Biochemical analyses were conducted to measure cortisol, brain-derived neurotrophic factor (BDNF), and acetylcholine (ACh) levels, using enzyme-linked immunosorbent assay (ELISA). Acetylcholine esterase (AChE) activity was measured by colorimetric method. Western blot analysis was performed to examine cAMP response element-binding protein (CREB) and phosphorylated CREB (p-CREB). Additionally, cytokine mRNA expressions were evaluated using polymerase chain reaction (PCR).RESULTS: Spontaneous alteration and discrimination ratio were decreased in SD group without treatment compared to the normal group (p<0.05). Similarly, BDNF was also decreased compared with normal group (p<0.05). Cortisol level and mRNA expression of TNF-α were increased significantly compared with normal group (p<0.05) along with a slight increase of ACh activity. Interestingly, NAC treatment mainly at the dose of 500 mg/kgBW prevented those pathological features significantly.CONCLUSION: NAC might prevent the SD-induced memory deficits by suppressing the inflammatory markers, activity of AChE, cortisol, and enhancing the level of BDNF.KEYWORDS: SD, NAC, memory, AChE, cortisol, inflammatory markers, BDNF
Platelet-rich plasma as an Alternative Supplement for Enhancing CD Marker Expression in Umbilical Cord-Derived Mesenchymal Stem Cells Prianggodo, Putra Agung; Septiana, Wahyunia Likhayati; Junaidi, Helsy; Astuti, Sastia Winda
Biology, Medicine, & Natural Product Chemistry Vol 14, No 1 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.141.249-257

Abstract

This study aimed to assess the effectiveness of platelet-rich plasma (PRP) and fetal bovine serum (FBS) as culture supplements for mesenchymal stem cells (MSCs) isolated from umbilical cord tissue. The study focused on the efficiency of xeno-free PRP in enhancing cell adhesion and proliferation and looked at cell surface markers for MSC characterization. Until the cells achieved 80% confluency, three repeats of FBS and PRP were added to the culture medium of mesenchymal stem cells derived from umbilical cord tissue in passage 10. Following harvesting, an assay kit for human mesenchymal stem cells was used to color the cells. Using flow cytometry, the surface markers CD73, CD90, CD105, and the negative marker CD44 were evaluated in the samples. Cells supplemented with PRP expressed more positive indicators and fewer negative markers. Whereas the results for CD90, CD105, and the negative marker were insignificant, the expression of CD73 increased significantly. PRP can enhance CD marker expression by acting as a substitute for FBS in the culture of xeno-free umbilical cord-derived MSCs.
Co-Authors Ahmad Aulia Ahmad Aulia Jusuf Antarianto, Radiana Dhewayani Astuti, Sastia Winda Atikah Chalida Barasila Barasila, Atikah Chalida Endang Rahmawati Fasha, Iqbal Fifinela Raissa Iqbal Fasha Jeannne Adiwinata Pawitan Junaidi, Helsy Lee, Hee Jae Melva Louisa Pawitan, Jeanne Adiwinata Prianggodo, Putra Agung Radiana Dhewayani Antarianto Raymond R. Tjandrawinata Retno Yulianti Rosyidah, Citra Tika Hamidah Apriliana Nai Warni Zulhija Putri Wawaimuli Arozal Yuni Setyaningsih