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All Journal Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science)
Wahjuningsih , Sri
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The Successful Artificial Insemination with Frozen Semen from Different Genetic Qualities of Friesian Holstein Bull Yekti, Aulia Puspita Anugra; Hasan, Ismail; Hermanto; Febrianto, Nanang; Isnaini, Nurul; Utami, Putri; Syah, Habib Asshidiq; Wahjuningsih , Sri; Susilawati, Trinil
Jurnal Ilmu-Ilmu Peternakan Vol. 34 No. 3 (2024): December 2024
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2024.034.03.15

Abstract

This study aimed to evaluate the success of artificial insemination (AI) using frozen semen with different genetic qualities of Friesian Holstein Bull. In this research, the bulls came from the artificial insemination center of Singosari, consisting of Friesian Holstein bulls with proven bull certificates and the pure breed from embryo transfer. The frozen semen was produced by standard semen processing in the artificial insemination center of Singosari. The inseminated female totals were 56 head of Friesian Holstein crossbred in Pujon district, Malang, Indonesia. The females were divided into two groups: 28 cows receiving insemination from frozen semen of proven bull (T1) and 28 from pure breed FH bull from embryo transfer (T2). The females were chosen with purposive sampling with the criteria, have a Body Condition Score (BCS) of at least 2.5 (scale 1–5), have normal reproductive organs, and exhibit the clear estrus sign. Artificial insemination was performed with a single dose at the eight hour after estrus. Semen deposition was at the corpus uteri, while the thawing process was performed at 22–23°C for 60 seconds. The result showed that the non-return rate 1 (NRR-1), non-return rate 2 (NRR-2), and conception rate (CR) values for AI using frozen semen from a proven bull were 96%, 75% and 43%, respectively. In contrast, the percentage of AI success using pure breed FH bull from embryo transfer was 93%, 86%, and 64%. In conclusion, AI success using pure breed FH bull from embryo transfer was better than proven bull frozen semen.
Optimization of BSA Column Gradient Concentrations on Post-Thaw Quality of Sexed Spermatozoa in Bali Bulls Yekti, Aulia Puspita Anugra; Setiawan, Raka; Ristrushana, Nurma Angelita; Susilawati, Trinil; Wahjuningsih , Sri; Isnaini, Nurul; Amalia, Anny; Sulistyowati, Deny; Pramudhita, Anggita Dian
Jurnal Ilmu-Ilmu Peternakan Vol. 35 No. 3 (2025): December 2025
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2025.035.03.13

Abstract

This study evaluated post-thaw semen quality and the proportions of X- and Y bearing spermatozoa in Bali cattle semen sexed by albumin of bovine serum albumin (BSA) column using two different gradients. In this study, frozen semen from a Bali bull was used, with three treatments: T0 (non-sexed frozen semen as a control), T1 (BSA 5%:8%), and T2 (BSA 5%:10%). The parameters observed were individual motility, viability, abnormality, concentration, total motile sperm (TSM), and sperm proportion.  The results showed that sperm motility in the upper fraction (UF) was highest under T2 (41.57%), whereas in the lower fraction (LF) it peaked under T1 (24.15%). Sperm viability was highest in the UF under T1 (57.69%) and in the LF under T1 (39.02%). The lowest abnormality was observed in the UF under T2 (4.64%) and in the LF under T1 (7.27%). Sperm concentration reached 30.14 million/straw in the UF under T2 and 22.27 million/straw in the LF under T2. Total sperm motility (TSM) was highest in the UF under T2 (12.57 million/straw) and in the LF under P1 (4.76 million/straw). The sperm proportions of the UF reached 75% X (T1) and the LF 81.71% Y (T1). Overall, the upper fraction obtained from the 5%:8% BSA gradient provided acceptable post-thaw sperm quality and adequate X/Y sperm enrichment, supporting its suitability for artificial insemination.   
Co-Authors Amalia, Anny Aulia Puspita Anugra Yekti Febrianto, Nanang Hermanto Ismail Hasan, Ismail Nurul Isnaini Pramudhita, Anggita Dian Putri Utami, Putri Ristrushana, Nurma Angelita Setiawan, Raka Sulistyowati, Deny Syah, Habib Asshidiq