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All Journal INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Journal of Biotropical Research and Nature Technology
Serang, Yitro
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Agriculture, Biological Sciences & Forestry Health Professions Social Sciences

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Molecular approach to the characterization of lipase encoding genes from Moraxella sp. SBE01 Soleha, Siti; Syarifah, Syarifah; Nurseha, Tito; Fatiqin, Awalul; Retnaningrum, Endah; Serang, Yitro
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 6 No 2 (2024): Promising and valuable research towards diagnosis, prognosis and treatment of dis
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v6i2.5596

Abstract

Lipase from Moraxella sp. SBE01 is an expression of the gene encoding lipase. Detection and characterization of the Moraxella sp. SBE01 lipase coding gene is necessary for large-scale lipase production through genetic engineering. This study aimed to observe the molecular weight, amino acid sequence, length, and conserved amino acids in the DNA encoding the lipase gene, with the goal of identifying and characterizing the lipase-coding gene from Moraxella sp. SBE01. The primer design process was conducted to amplify the lipase gene from Moraxella sp. SBE01 using specialized software for sequence alignment and phylogenetic analysis. Amplification was carried out using PCR with the designed primer, forward primer (GTC ATG ATG TAC TTC CAY GGN GGN GG), reverse primer (GGT TGC CGC CGG CDS WRT CNC C). PCR was carried out under pre-denatured conditions at 95°C (3 minutes), followed by 30 cycles of denaturation at 95°C, annealing at 66°C (30 seconds), 70°C elongations (1 minute) and final elongation of 70°C (10 minutes). The PCR results were electrophoresed using 1% agarose gel with a 1 kb DNA marker. The PCR results were sequenced and analyzed for gene and amino acid sequences and the type of lipase expressed. Sequencing resulted in 387 bp of the nucleotide sequence. The gene and amino acid sequences from Moraxella sp. SBE01 had high homology with the gene and amino acid sequences from Moraxella sp. strain TA144. The lipase gene encodes a protein consisting of 129 amino acids and contains a conserved HGG (His-Gly-Gly) motif, which is characteristic of lipases in family IV, also known as the hormone-sensitive lipase (HSL) family. This conserved sequence suggests that the lipase shares structural and functional similarities with other enzymes in the HSL family, playing a key role in lipid metabolism.
Food Safety Alert: Traces of Formalin and Rhodamine B Found in Children's Snacks Near Schools in Lubuklinggau, South Sumatra Agustina , Sephia Eka Putri; Devira , Yolanda; Tiwi , Irma Abellia Agus; Serang, Yitro; Novita Sunarti, Riri; Syafri , Ronny; Velina, Yessy
Journal of Biotropical Research and Nature Technology Vol. 3 No. 2 (2025): Borneo
Publisher : Prodi Biologi FMIPA Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52850/borneo.v3i2.16590

Abstract

Security food is an important global issue, especially in the context of snack child school. The research aims to test the existence of content material addition food, such as formalin and rhodamine B, in snacks school at Public Elementary School 58 Lubuklinggau City. Testing was done using descriptive qualitative with a special test kit to detect formalin and rhodamine B content in various types of snacks. Research results show that all sample snacks from Elementary School 58 Lubuklinggau City negative contain formalin and rhodamine B. This is to signify that snacks sold at SDN 58 Lubuklinggau City are safe for consumption by children, but education for the community still needs to be done to prevent children from snacking carelessly. Education this can give through inspection laboratory tours and activities Communication, Information and Education (KIE) by the POM Center in Lubuklinggau City has contributed positively to increasing awareness among​ the public about choosing safe food​ for children in school.
Co-Authors Agustina , Sephia Eka Putri Devira , Yolanda Fatiqin, Awalul Nurseha, Tito Retnaningrum, Endah Riri Novita Sunarti, Riri Soleha, Siti Syafri , Ronny Syarifah Syarifah, Syarifah Tiwi , Irma Abellia Agus Yessy Velina