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All Journal Prosiding Seminar Biologi Jurnal Florea Sains Natural: Journal of Biology and Chemistry Indonesian Journal of Applied Research (IJAR)
Nia Yuliani
Program Studi Biologi, Universitas Nusa Bangsa
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT

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OPTIMASI WAKTU PENGERINGAN TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL RIMPANG TEMU PUTIH (Curcuma aromatica Salisb) Nia Yuliani; Mamay Maslahat; Puji Lestari
JURNAL SAINS NATURAL Vol. 4 No. 2 (2014): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (721.426 KB) | DOI: 10.31938/jsn.v4i2.86

Abstract

Optimization of The Drying Time of The Anti-Oxidant Activity of The Ethanol Extract of The Rhizomes of Temu Putih (Curcuma aromatica Salisb)Indonesia is a tropical country that has many varieties of medicinal herbal plants. One of them is Curcuma aromatica Salisb. It has a secondary metabolite that acts as an antioxidant. The metabolite will degrade if it goes through a long drying process. The Curcuma aromatica Salisb rhizome is dried at a temperature of 65oC with variant time durations : 22, 23, 24, 25 and 26 hours. The dried rhizome is extracted with ethanol as the solvent and the results are 14.16%, 11,89%, 15,95%, 12,10% dan 11,97% respectively. Phytochemical test shows the existence of alkaloids and flavonoids in the extract. The measurement of antioxidant activity is done using the DPPH method with a spectrophotometer at 517 nm wavelength. The result is then compared to Quercetin as the positive control. The IC50  values of Quercetin, extracts of Curcuma after 22, 23, 24, 25 and 26 hours after drying respectively are 6,47µ g/ml , 160,54µ g/ml, 133,17µ g/ml, 117,81µ g/ml, 144,69 µ g/ml, dan 157,7 µ g/ml. The extracts after 23-25 hours of drying have medium antioxidant levels, while the ones with 22 and 26 hours of drying have low antioxidant levels. The duration of drying affects the antioxidant activity of Curcuma aromatica salisb extract. The highest value of IC50 is achieved on the positive control of Quercetin and followed by the Curcuma extract with 24 hours of drying.Key words : ethanol, Curcuma aromatica salisb, drying duration, antioxidant activity ABSTRAKIndonesia merupakan negara tropis yang memiliki berbagai macam tanaman obat. Salah satunya adalah temu putih (Curcuma aromatica Salisb). Temu putih mengandung senyawa metabolit sekunder yang bermanfaat sebagai antioksidan. Senyawa metabolit sekunder akan rusak bila dikeringkan dengan waktu yang lama.Rimpang temu putih dikeringkan pada suhu 65oC dengan variasi waktu 22, 23, 24, 25 dan 26 jam. Simplisia temu putih diekstrak dengan pelarut etanol diperoleh rendemen berturut-turut, 14,16%, 11,89%, 15,95%, 12,10% dan 11,97%. Uji Fitokimia menunjukkan adanya alkaloid dan flavonoid pada ekstrak etanol rimpang temu putih. Penentuan aktivitas antioksidan dilakukan dengan metode DPPH menggunakan spektrofotometer pada panjang gelomban g 517 nm. Hasilnya dibandingkan dengan Kuersetin sebagai kontrol positif. Nilai IC50 Kuersetin, ekstrak etanol rimpang temu putih 22, 23 24, 25 dan 26 jam berturut-turut adalah 6,47μg/ml , 160,54μg/ml, 133,17μg/ml, 117,81μg/ml, 144,69 μg/ml, dan 157,7 μg/ml. Ekstrak etanol rimpang temu putih pengeringan 23-25 jam memiliki aktivitas antioksidan sedang, sedangkan pengeringan 22 dan 26 jam memiliki aktivitas antioksidan lemah. Lama proses pengeringan mempengaruhi aktivitas antioksidan ekstrak etanol rimpang temu putih. IC50 terbaik diperoleh pada standar positif Kuersetin kemudian pada ekstrak etanol rimpang temu putih pengeringan 24 jam.Kata Kunci : etanol, Curcuma aromatica Salisb, lama pengeringan, aktivitas antioksidan
HIDROLISIS ASAM PADA TEPUNG PATI UBI JALAR PUTIH (Ipomoea batatas L.) DALAM PEMBUATAN GULA CAIR RTM Sutamihardja; Nia Yuliani; Hana Laelasari; Devy Susanty
JURNAL SAINS NATURAL Vol. 6 No. 2 (2016): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (494.161 KB) | DOI: 10.31938/jsn.v6i2.163

Abstract

Acid Hydrolysis on The Starch Flour of white sweet potato (Ipomoea batatas L.) in Making of Liquid SugarNational sugar needs for both direct consumption and for industrial needs will continue to increase as the population increases. According to Dirjenbun, in 2014 the national sugar demand reaches 5.7 million tons. Consisting of 2.8 million tons of white crystalline sugar for direct community consumption and 2.9 million tons of refined crystal sugar to meet industrial needs. White sweet potato can be used as raw material for making liquid glucose through hydrolysis process with acid (HCl). The preparation of liquid glucose consists of two stages: gelatinization stage and hydrolysis stage. Optimum hydrolysis was determined by variations in HCl concentration of 0.25; 0.5; and 0.75 N and time variations of 30, 60, and 90 minutes. The yield of sweet potato starch was 28.82% and the highest yield of hydrolysis result of white sweet potato starch was 94.07% at acid concentration 0.75 N with hydrolysis time 90 minutes.Keywords: white sweet potato, Flour of white sweet potato, liquid sugar, acid hydrolysisABSTRAKKebutuhan gula nasional baik untuk konsumsi langsung maupun untuk kebutuhan industri akan terus meningkat sejalan dengan meningkatnya jumlah penduduk. Menurut Dirjenbun, pada tahun 2014 kebutuhan gula nasional mencapai 5,7 juta ton yang terdiri dari 2,8 juta ton gula kristal putih untuk konsumsi masyarakat langsung dan 2,9 juta ton gula kristal rafinasi untuk memenuhi kebutuhan industri. Ubi jalar putih dapat dijadikan bahan baku pembuatan glukosa cair melalui proses hidrolisis dengan asam (HCl). Pembuatan glukosa cair terdiri dari dua tahap yaitu tahap gelatinisasi dan tahap hidrolisis. Hidrolisis optimum ditentukan dengan variasi konsentrasi HCl yaitu 0,25; 0,5; dan 0,75 N dan variasi waktu 30, 60, dan 90 menit. Rendemen pati ubi jalar didapatkan sebesar 28,82% dan rendemen glukosa tertinggi hasil hidrolisis pati ubi jalar putih sebesar 94,07% pada konsentrasi asam 0,75 N dengan waktu hidrolisis 90 menit.Kata kunci: Ubi jalar putih, Tepung Pati Ubi Jalar Putih, Gula cair, hidrolisis asam
AKTIVITAS ANTIBAKTERI INFUSA DAUN LIDAH BUAYA (Aloe vera L) TERHADAP PERTUMBUHAN Staphylococcus aureus DAN Escherichia coli Yuni Widyastuti; Nia Yuliani; I.G.A. Manik Widhyastini
JURNAL SAINS NATURAL Vol. 6 No. 1 (2016): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (537.015 KB) | DOI: 10.31938/jsn.v6i1.253

Abstract

Antibacterial Activity of Infused Aloe Vera Leaf (Aloe Vera L) on Growth Of Stapylococcus aureus and Escherichia coliDisinfectants may act as antibacterials. Aloe vera (Aloe vera L) is one of the plants that is often used by the community as a medicinal plant that has many active compounds such as lignin, saponin and anthraquinone substances that act as antibacterial. This study aims to determine the antibacterial activity of Aloe vera leaf extracted with infusa method on the growth of Staphylococcus aureus and Escherichia coli bacteria. The research method is to see the reduction / reduction of bacterial count (% reduction) after 30 second and 60 second with disc diffusion method. The results showed decrease or decrease of bacteria count (% reduction) to Escherichia coli bacteria found in aloe leaf skin infected by 28,2% after 60 seconds. While decrease or decrease of bacteria amount (% reduction) to Staphyloccocus uareus bacteria infusa mixture of meat and skin of Aloe vera leaves of 27.5% after 60 seconds. Antibacterial test of aloe vera leaf aloe (Aloe veraL) of 100% concentration by disc diffusion method did not show any inhibition zone around cultures of test bacteria.Keywords: Aloe vera, antibacterial activity, Staphylococcus aureus, and Escherichia coli, Infusa.ABSTRAKDesinfektan dapat berfungsi sebagai antibakteri. Lidah buaya (Aloe vera L) merupakan salah satu tanaman yang sering digunakan masyarakat sebagai tanaman obat yang memiliki banyak senyawa aktif seperti lignin, saponin dan antrakuinon yaitu zat yang berfungsi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri daun lidah buaya (Aloe vera L) yang diekstrak dengan metode infusa terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli. Metode penelitian yaitu dengan melihat penurunan/pengurangan jumlah bakteri (% reduksi) setelah waktu 30 detik dan 60 detik dengan metode difusi cakram. Hasil penelitian menunjukkan penurunan atau pengurangan jumlah bakteri (% reduksi) terhadap bakteri Escherichia coli terdapat pada infusa kulit daun lidah buaya yaitu sebesar 28,2 % setelah waktu 60 detik.Sedangkan penurunan atau pengurangan jumlah bakteri (% reduksi) terhadap bakteri Staphyloccocus uareus terdapat pada infusa campuran daging dan kulit daun lidah buaya yaitu sebesar 27,5 % setelah waktu 60 detik. Uji antibakteri infusa daun lidah buaya (Aloe veraL) konsentrasi 100% dengan metode difusi cakram tidak menunjukkan adanya zona penghambatan disekitar biakan bakteri uji.Kata kunci : Lidah buaya, Aktivitas antibakteri, Staphylococcus aureus, dan   Escherichia coli,   Infusa.
ANALISIS PROKSIMAT DAN KEKUATAN GEL AGAR – AGAR DARI RUMPUT LAUT KERING PADA BEBERAPA PASAR TRADISIONAL Nia Yuliani; Noviana Maulinda; RTM Sutamihardja
JURNAL SAINS NATURAL Vol. 2 No. 2 (2012): Sains Natural (Edisi Khusus)
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (449.018 KB) | DOI: 10.31938/jsn.v2i2.40

Abstract

Proximate and Gel Strenght Analysis of Agar from Dry Seaweed in some Traditional Markets          Seaweeds are good material in processing to intermedite and finish product like agar – agars  in Indonesia still in a semi-modern and traditional methods. This led Indonesia still make import the agar gelatin from other countries. The purpose of the study was to increas use the dried seaweed in traditional markets for the products of gelatin and to provide the nutrition information, like water content, ash content, crude fat, protein, carbohydrates, crude fiber, the levels of sulfate and gel strength of processed gelatin from dry seaweed. Dried seaweed used in this study, obtained from a traditional market in Bogor, Bekasi and Jakarta. Proximate result and the gel strength from extracted agar powder samples would compared with commercial flour agar. Research results obtained extracted agar – agar powder samples had lower chemical content and gel strength than the commercial agar, but it still could be used as food, although in very small amounts.Keywords : dry seaweeds, proximate analysis, gel strength, agar – agar  ABSTRAK          Pengolahan rumput laut menjadi bahan olahan seperti agar – agar di Indonesia masih secara tradisional dan semi modern. Hal ini menyebabkan Indonesia masih mengimpor agar – agar dari negara lain. Manfaat dan tujuan penelitian ini adalah memanfaatkan rumput laut kering yang banyak terdapat di pasar – pasar tradisional menjadi produk agar – agar dan memberikan informasi kandungan nutrisi yang berupa air, abu, lemak kasar, protein, karbohidrat, serat kasar, kadar sulfat serta kekuatan gel dari agar –agar hasil olahan rumput laut kering. Rumput laut kering yang digunakan pada penelitian ini, diperoleh dari  pasar tradisional di Bogor, Bekasi dan Jakarta. Hasil proksimat dan kekutan gel dari sampel tepung agar – agar hasil ekstrak kemudian dibandingkan dengan tepung agar – agar komersial berdasarkan hasil penelitian diperoleh sampel tepung agar – agar hasil ekstraksi dengan kandungan kimia dan kekuatan gel yang lebih rendah dari agar – agar komersial, tetapi masih dapat dimanfaatkan sebagai bahan pangan walaupun dalam jumlah yang sedikit.Kata kunci : rumput laut kering, analisis proksimat, kekuatan gel, agar – agar
BENTONIT TERAKTIVASI ASAM SULFAT SEBAGAI ADSORBEN DALAM PEMURNIAN PELUMAS BEKAS Tiva Lathifah; Nia Yuliani; Gladys Ayu Paramita Kusumah Wardhani
JURNAL SAINS NATURAL Vol. 9 No. 1 (2019): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (462.537 KB) | DOI: 10.31938/jsn.v9i1.170

Abstract

Activated Bentonits of Sulfatic Acid as Adsorben in Purchase of Used Lubricants Recycling of used lubricating oil is one of the alternatives in the framework of efficiency, saving oil consumption, and reducing pollution. One effort to purify used lubricating oil is to separate impurities through the adsorption method. The adsorbent that can be used is bentonite. Activation of bentonite using acid will produce adsorbent with an active side and greater surface acidity so that the adsorption ability is higher than before activated. Characteristics of lubricating oil produced are: kinematic viscosity 40 °C and 100 °C at 109.94 cSt and 14.57 cSt recently; viscosity index is 136; specific gravity 15 °C is 0.8872; and the resulting color is L5.0. Activated sulfonic bentonite can be an optimum adsorbent in purifying used lubricating oil, with optimum bentonite concentration is 30% and optimum adsorption temperature is 70 °C resulting in a 49% increase in viscosity efficiency of 40 °C and 30.79% for temperatures of 100 °C.Keywords: Bentonite, Lubricants, Adsorption  ABSTRAK Daur ulang minyak pelumas bekas merupakan salah satu alternatif dalam rangka efisiensi, penghematan konsumsi minyak bumi, serta mengurangi pencemaran. Salah satu upaya menjernihkan minyak pelumas bekas adalah dengan memisahkan zat-zat pengotor melalui metode adsorpsi. Adsorben yang dapat digunakan adalah bentonit. Aktivasi bentonit menggunakan asam akan menghasilkan adsorben dengan sisi aktif dan keasaman permukaan yang lebih besar sehingga kemampuan adsorpsinya lebih tinggi dibandingkan dengan sebelum  diaktivasi. Karakteristik minyak pelumas yang dihasilkan yaitu: viskositas kinematik 40 °C  dan 100 °C  sebesar 109,94 cSt dan 14,57 cSt secara berturut-turut; indeks viskositas sebesar 136; specific gravity 15 °C  sebesar 0,8872; serta warna yang dihasilkan adalah L5,0. Bentonit teraktivasi asam sulfat mampu menjadi adsorben yang optimum dalam pemurnian minyak pelumas bekas, dengan  konsentrasi bentonit optimum adalah 30% dan suhu adsorpsi optimum adalah 70 °C menghasilkan % efisiensi kenaikan viskositas  sebesar 49,15% untuk suhu 40 °C dan 30,79% untuk suhu 100 °C.Kata kunci : adsorpsi, bentonit, pelumas
Preparation, Characterization and Phenol Adsorption of Mangifera kemanga Blume Seed Siti Hadiati Mardiah; Dian Arrisujaya; Devy Susanty; Nia Yuliani
JURNAL SAINS NATURAL Vol. 12 No. 3 (2022): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1392.589 KB) | DOI: 10.31938/jsn.v12i3.410

Abstract

The potential of Mangifera kemanga Blume., an inexpensive biosorbent, for removing of hazardous substances such as phenols from its aqueous solution has been studied. The authors used Scanning Electron Microscope (SEM), Fourier Transform Infrared (FTIR) Spectrometers, and quantification to study the morphology and characterization of Mangifera kemanga Blume. seeds (MKS) biomass, as well as batch experiments to determine the percentage of phenol removed when pH, contact period, biosorbent dosage, and phenol concentration were varied. The Langmuir, Freundlich, Temkin, and Dubinin–Radushkevich isotherm models had been used to interpret the experimental results. The optimal values found in our research correspond to a pH of 6 for an MKS dosage of 35 g/L and a contact time of 45 minutes for initial phenol concentrations ranging from 5 to 25 mg/L.  The result indicated that MKS was a particularly successful adsorbent for phenol chemisorption from aqueous solution.Keywords: adsorption;  endemic; Bogor; Mangifera kemanga; phenol
Diversity of Mammals in 8 Years of Jati (Tectona grandis Linn. f) Unggul Nusantara (JUN) Tun Susdiyanti; Ken Dara Cita; Nia Yuliani; Noval Diantama; Dwi Agus Sasongko
JURNAL SAINS NATURAL Vol. 13 No. 1 (2023): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31938/jsn.v13i1.395

Abstract

Mammals had several ecological functions and roles. The existence of mammals plays an essential role in the balance of natural ecosystems. The Experimental garden in Cogreg Village is one of the possible places to become a habitat for a diversity of mammals. The diversity of mammals found and identified in the Cogreg Experimental Garden consists of 6 types: Pteropus vampyrus, Tupaia javanica, Callosciurus notatus, Rattus argentiventer, Herpestes javanicus, and Suncus murinus, with a total of 165 individuals. The mammal habitat found in the Cogreg Experimental Garden is secondary forest or JUN Forest (Tectona grandis Linn. f) with INP Index of 195.91%.Keywords:  Diversity;  Mammals;  Cogreg; HabitatKeanekaragaman Mamalia pada Tegakan Trubusan Jati (Tectona grandis Linn. f) Unggul Nusantara (JUN) Umur 8 TahunABSTRAKMamalia memiliki beberapa fungsi dan peran ekologis. Keberadaan mamalia sangat berperan penting dalam keseimbangan ekosistem alam. Kebun Percobaan di Desa Cogreg adalah salah satu tempat yang memungkinkan untuk menjadi habitat bagi keanekaragaman mamalia. Keanekaragaman mamalia yang ditemukan dan teridentifikasi di Kebun Percobaan Cogreg terdiri dari 6 jenis mamalia Pteropus vampyrus, Tupaia javanica, Callosciurus notatus, Rattus argentiventer, Herpestes javanicus, dan Suncus murinus dengan jumlah total keseluruhan 165 individu. Habitat mamalia yang terdapat di Kebun Percobaan Cogreg yaitu hutan sekunder atau hutan tegakan Jati (Tectona grandis Linn. f) Unggul Nusantara (JUN) dengan indeks nilai penting (INP) sebesar 195,91%.Kata Kunci: Keanekaragaman; Mamalia; Cogreg; Habitat
Hemoglobin Levels and Number of Erythrocytes in Scabies-infected Male Mice (Mus musculus) Treated with Water Extract of Taro (Colocasia esculenta (L.) Schott Cultivar Hideung Widhyastini, I Gusti Ayu Manik; Nia Yuliani; Ananda Sarah Nur Azizah
Sains Natural: Journal of Biology and Chemistry Vol. 14 No. 1 (2024): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31938/jsn.v14i1.694

Abstract

This study aims to analyze the hemoglobin levels and number of erythrocytes in scabies-infected male mice treated with taro water extract as a cheap and safe alternative treatment for scabies. The test animals were 24 mice weighing 20-25 grams, divided into 6 groups consisting of: K1: taro water extract 100gr/100 ml water, K2 taro water extract 200gr/100ml water, K3: using 300gr/100ml taro water extract water, K4: ivermectin positive control, K5: negative control with water alone, and K6 normal control not infected with scabies and not given treatment. The application of taro extract was carried out for five days. Observations were carried out every day, until day 7. Blood samples were taken from mice three times: before being infected with scabies (healthy), when infected with scabies, and after being treated with taro water extract. The data obtained was processed statistically using the SPSS software with the One Way ANOVA test. Sarcoptes scabiei infection affects the appearance of the blood profile of male mice when infected, there is an increase in the number of erythrocytes but does not affect hemoglobin levels. Treatment with water extract of the taro (Colocasia esculenta (L.) Schott) cultivar hideung in different concentrations did not show significant blood results, but it could heal wounds caused by scabies infection.
Co-Authors Aditya Prihantara Agus Susanto Alifah Nur Anzani Amry Syawaalz Ananda Sarah Nur Azizah Cahyadi Cahyadi Devy Susanty Dian Arrisujaya Dwi Agus Sasongko Gladys Ayu Paramita Kusumah Wardhani Hana Laelasari Heri Susanto I Gusti Ayu Manik Widhyastini Irfan Martiansyah Ken Dara Cita Mamay Maslahat Mawaddah Lisna Meilinda Pramleonita Mia Azizah Nentin Surtini Noval Diantama Noviana Maulinda Oktavio Rosani Ridha Arizal Rr Pramitha Ika Putri Ayuningtyas RTM Sutamihardja RTM Sutamiharja SATRIYAS ILYAS Siti Hadiati Mardiah Srikandi, Srikandi Supriyono Eko Wardoyo Tiva Lathifah Tun Susdiyanti Yuni Widyastuti