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Pangestu, Kukuh Waseso Jati
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Metabolite Profile of Marine Sponge (Stylissa sp.) Lipid Extract and Its Effect on Bacterial Skin Infection Maulana, Farreh Alan; Utami, Ni Wayan Putri; Handayani, Ervina; Kabir, Mila Mayanti; Inggit, Baiq Putri Maharani Bine; Pangestu, Kukuh Waseso Jati; Deccati, Rizqa Fersiayana; Dewi, Ni Made Amelia Ratnata; Sunarwidhi, Anggit Listyacahyani
Jurnal Biologi Tropis Vol. 24 No. 1b (2024): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1b.7848

Abstract

UV radiation from sunlight can induce the formation of free radicals, which can disrupt cellular homeostasis or DNA by triggering inflammatory signal transduction. The occurrence of inflammation in the skin can be aggravated by the presence of the bacterium Staphylococcus aureus. Previous studies have shown that the marine sponge Stylissa sp. contains an abundance of fatty acids and lipids compared to other species. Although Stylissa sp. has great potential for health applications, research on the bioactivity of lipid and fatty acid compounds from this sponge remains limited. Therefore, this study aims to explore the lipid content of Stylissa sp. as an alternative source of antibacterial agents against pathogens responsible for photoaging. The metabolite profiling of the extract was conducted using GC-MS, while the antibacterial activity was performed using the Kirby-Bauer method. Based on GC-MS profiling results, six compounds were identified in the lipid extract of Stylissa sp. namely palmitic acid, butyl glycol acetate, n-eicosylcyclohexane, isopropyl laurate, oleic acid, and 1-tetradecanol. Antibacterial evaluation of Stylissa sp. lipid extract at concentrations of 25%, 75%, and 100% showed antibacterial activity against Staphylococcus aureus, with inhibition zone diameters of 1.83, 2.17, and 2.92 mm, respectively. The results in this study have shown that the lipid extract of Stylissa sp. contains lipid compounds with potential anti-bacterial activity towards S. aureus. Future research to isolate unsaturated fatty acid compounds from the lipid extract of Stylissa sp. to achieve higher antibacterial activity is recommended.
Comparison of Anti-Bacterial Activity of Methanol Extract and Non-Polar Fraction of Macroalgae Sargassum sp. Wibowo, Adi Tri; Widyastuti, Sri; Prasedya, Eka Sunarwidhi; Mukhlishah, Neneng Rachmalia Izzatul; Pangestu, Kukuh Waseso Jati; Sunarwidhi, Anggit Listyacahyani
Jurnal Biologi Tropis Vol. 25 No. 4 (2025): Oktober-Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4.10522

Abstract

Sargassum sp. is an abundant source of bioactive non-polar compounds, however their anti-bacterial potential remains understudied. This study aimed to compare the anti-bacterial activity between the methanol extract and non-polar fraction of Sargassum sp. Extraction was performed using sonication with methanol, followed by partition with petroleum ether and fractionation on silica gel G60 using n-hexane:ethyl acetate (9:1). FTIR analysis characterized the functional groups, while anti-bacterial evaluation was performed using the disc diffusion method against Staphylococcus aureus. Data were analyzed statistically using one-way ANOVA. FTIR spectrum of the methanol extract showed absorption bands corresponding to non-polar compounds. In the non-polar fraction, the bands were more dominant, suggesting a higher content of non-polar compounds. Anti-bacterial evaluation of Sargassum sp., methanol extract and non-polar fraction exhibited inhibitory effects against S. aureus. The non-polar fraction produced a larger inhibition zone compared to the methanol extract, indicating stronger anti-bacterial activity and significant difference between the two samples (p<0.05). The results of this study showed that the non-polar fraction of Sargassum sp. methanol extract has higher anti-bacterial activity compared to the extract. Further studies are recommended to elucidate the individual bioactive compounds and identify their anti-bacterial mechanisms.
Co-Authors Deccati, Rizqa Fersiayana Ervina Handayani Inggit, Baiq Putri Maharani Bine Kabir, Mila Mayanti Maulana, Farreh Alan Mukhlishah, Neneng Rachmalia Izzatul Ni Made Amelia Ratnata Dewi Prasedya, Eka Sunarwidhi Sri Widyastuti Sunarwidhi, Anggit Listyacahyani Utami, Ni Wayan Putri Wibowo, Adi Tri