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Vaidika, I Komang Satya
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Antibacterial Activity Test of Sumbawa White Honey against Escherichia coli ATCC 25922 Hulfifa, Lale Nandita; Rosyunita, Rosyunita; Wardoyo, Eustachius Hagni; Hasbi, Nurmi; Rahim, Adelia Riezka; Arnawati, Ida Ayu; Alamsyah, Abiel Dwi Cahya Firdaus; Vaidika, I Komang Satya; Hazaa, Saskia Safarina
Jurnal Biologi Tropis Vol. 24 No. 3 (2024): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i3.7407

Abstract

Due to their high mortality and morbidity rates, E. coli infections are a serious issue. Antimicrobial Resistance (AMR) is a result of this indiscriminate antibiotic use. One approach to address this issue is to utilize natural antimicrobials such as Sumbawa white honey. Sumbawa white honey contains antibacterial components like glucose and fructose (80%), water (17%), polyphenols, and hydrogen peroxide. This study aimed to analyze the antibacterial activity of white honey from Sumbawa against E. coli ATCC 25922. The research method employed was a laboratory experiment using a post test only control group design. Antibacterial testing was conducted using disc diffusion at concentrations of 25%, 75%, and 100%, with chloramphenicol as positive control and DMSO as negative control, and honey processing involved dilution. Antibacterial tests were repeated five times  and antibacterial activity was observed based on the diameter of inhibition zones around the disc. The outcomes showed that there was a restraint zone at 100 percent grouping of honey, to be specific 0.90 mm, while at 25% and 75% fixations there were no hindrance zones. According to the findings of this study, white honey from Sumbawa has a low antibacterial activity (0.90 mm), but this activity is ineffective against E. coli ATCC 25922. Further identification of the chemical components in Sumbawa white honey that are primarily responsible its antibacterial effect.
Antibacterial Activity Test of White Honey from Sumbawa NTB Againts Staphylococcus epidermidis Vaidika, I Komang Satya; Wardoyos, Eustachius Hagni; Rahim, Adelia Riezka
Jurnal Biologi Tropis Vol. 24 No. 4 (2024): Oktober - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i4.7750

Abstract

Staphylococcus epidermidis is the most common bacteria found on human skin that causes acne and is a coagulase-negative Staphylococcus species (CoNS). Resistance to methicillin is widespread, especially in Staphylococcus epidermidis bacteria or often called MRSE and is widespread globally ranging from 75-90%. Regarding antibiotic resistance, it is important to do research to find new alternatives to this problem. One of the natural antibiotics that can be used is honey which has benefits as an antibacterial because it has three systems that play a role in inhibiting bacterial growth, namely osmotic pressure, acidity and inhibine. The purpose of this study was to analyze the antibacterial effect of Sumbawa white honey on Staphylococcus epidermidis bacteria. The method used in this study was an analytical laboratory experiment with a Randomized Controlled Trial (RCT) type of post test only design. The method of making honey using disc diffusion was carried out 5 times at concentrations of 25%, 75%, 100%, positive chloramphenicol control and negative DMSO control. The results of the antibacterial test can be seen from the formation of the diameter of the inhibition zone on the disc paper. The results of the study obtained that at a concentration of 25% no inhibition zone could be formed, while in the 75% study an inhibition zone of 2.32 mm was formed and 100% of 6.71%. Based on the results of the antibacterial activity test, it can be concluded that the study on Sumbawa white honey at concentrations of 75% and 100% is included in the resistant/weak category and the activity is not effective in inhibiting the growth of S. epidermidis.
Adolescent Idiopatic Scoliosis: A Literatur Review Az-zahra, Falira Khoirunnisa; Fadila, Amila; Zahira, Lu’lu Al Fatina; Firdaus A, Abiel Dwi Cahya; Fadlurrohman, M. Zaidan; Anindya M, Nyoman Ayu; Aryuni, Baiq Aisha; Vaidika, I Komang Satya; Ramadhita, Nova Izza Salsa; Putra, Rian Akka Jaya; Akbar, Rauchan Abdi; Alaydrus, Muhammad Mukaddam
Jurnal Biologi Tropis Vol. 24 No. 1b (2024): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1b.7961

Abstract

Adolescent Idiopathic Scoliosis (AIS) is a structural spinal disorder commonly found in adolescents, characterized by an unknown cause of lateral curvature. This condition is typically detected during periods of rapid growth, particularly between the ages of 10 and 18. This literature review aims to provide a deeper understanding of adolescent idiopathic scoliosis in general, as well as to explore the latest management criteria for adolescent idiopathic scoliosis. The earlier AIS is detected and managed, the better the prognosis for the patient. The method used in this paper is a literature review. This review method involves searching for and examining reading Scholar, PubMed, and ProQuest regarding AIS. This paper summarizes the etiology, epidemiology, clinical manifestations, classification, diagnosis, and available treatments including management and rehabilitation currently available.
Co-Authors Akbar, Rauchan Abdi Alamsyah, Abiel Dwi Cahya Firdaus Alaydrus, Muhammad Mukaddam Anindya M, Nyoman Ayu Arnawati, Ida Ayu Aryuni, Baiq Aisha Az-Zahra, Falira Khoirunnisa Eustachius Hagni Wardoyo Fadila, Amila Fadlurrohman, M. Zaidan Firdaus A, Abiel Dwi Cahya Hasbi, Nurmi - Hazaa, Saskia Safarina Hulfifa, Lale Nandita Putra, Rian Akka Jaya Rahim, Adelia Riezka Ramadhita, Nova Izza Salsa Rosyunita, Rosyunita Wardoyos, Eustachius Hagni Zahira, Lu’lu Al Fatina