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Isolasi bakteri asam laktat asal feses bayi dan potensinya dalam menghambat pertumbuhan Escherichia coli Hasbi, Nurmi; Rosyunita; Rahim, Adelia Riezka; Ayunda, Rahmah Dara; Parwata, Wayan Sulaksmana Sandhi; Farras, Afif; Raihan, Al Fikar; Billah, Muhammad Azim
Jurnal Kedokteran Universitas Palangka Raya Vol 12 No 1 (2024): Jurnal Kedokteran Universitas Palangka Raya
Publisher : Fakultas Kedokteran, Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37304/jkupr.v12i1.12852

Abstract

Lactic acid bacteria (LAB) play an important role in improving performance and mitigating disease because of their ability to maintain the physiological balance of the gastrointestinal tract and resistance to pathogenic bacteria. LAB is one of the bacteria found in the digestive tract. LAB must be isolated from their hosts to increase their efficiency as probiotic ingredients. This study aims to obtain local isolates of LAB a from baby feces through phenotypic identification and antibacterial test. The sample used in the research was the feces of babies aged 1-6 months that patients from Mataram University Hospital. Isolation was carried out by growing bacteria from feces using the Total Plate Count method on MRSA media plus CaCO3. Phenotypic characterization is morphology test, gram stain and biochemical tests. The results of bacterial isolation obtained 8 isolates of LAB. LAB produced a clear zone in the MRSA media, because these bacteria were able to secrete acid in the MRSA. The results of gram staining show that all isolates are gram positive bacteria. Based on the morphology of LAB had characteristic 6 isolates of cocci and 2 isolates of bacilli. All isolates was able to produce an inhibition zone against E. coli with 3 isolates in the moderate category and 5 isolates in the weak category. The best inhibition zone in the medium category is for cocci-shaped bacteria.The existence of LAB isolates from baby feces can be used as a reference source for further research on probiotics such as other antibacterial test
Aktivitas antibakteri propolis madu putih sumbawa terhadap pertumbuhan Staphylococcus epidermidis, Staphylococcus aureus dan Escherichia coli Wardoyo, Eustachius Hagni; Rosyunita; Rahim, Adelia Riezka; Arnawati, Ida Ayu; Haza, Saskia Safarina; Hulfifa, Lale Nandhita; Alamsyah, Abiel Dwi Cahya Firdaus; Validika, I Komang Satya; Hasbi, Nurmi
Jurnal Kedokteran Universitas Palangka Raya Vol 12 No 1 (2024): Jurnal Kedokteran Universitas Palangka Raya
Publisher : Fakultas Kedokteran, Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Silent pandemic is a health issue related to antimicrobial resistance. Currently, deaths due to antimicrobial resistance in the world reach 700 thousand people per year and it is predicted that by 2050 it could reach 10 million people. So exploring new alternative sources is a must. One ingredient that is often used as an infection medicine and health supplement is honey propolis. Sumbawa white honey propolis is a biological product that has not yet been widely explored for its potential as an antibacterial. The research aims to determine the phytochemical content and antibacterial potential of Sumbawa white honey propolis against Staphylococcus epidermidis, Staphylococcus aureus and Escherchia coli. This research is a true experiment research with a post-test only control group design. Propolis is extracted by maceration with propylene glycol as a solvent. The concentrations of propolis extract used are 100%, 75% and 50%. Chloramphenicol positive control and 10% DMSO negative control. The antibacterial test was detected in five replications on Mueller Hinton Agar media using the disk diffusion method by measuring the diameter of the inhibition zone. Data were analyzed statistically using the OneWay Anova test. Sumbawa white honey propolis contains flavonoids, tannins and triterpenoids. The results showed that propolis was unable to inhibit gram-negative E. coli bacteria with an inhibition zone diameter of 0 mm. Different results in gram positives, Sumbawa white honey propolis was able to inhibit the growth of S. epidermidis and S. aureus. The results showed that propolis was unable to inhibit gram-negative E. coli bacteria with an inhibition zone diameter of 0 mm. Different results in gram positives, Sumbawa white honey propolis was able to inhibit the growth of S. epidermidis and S. aureus. However, Sumbawa white honey propolis against both gram-positive bacteria was in the weak category compared to the positive control. This is because all the Sumbawa white honey propolis concentration series have an inhibitory zone diameter of ≤ 5 mm.
Isolasi Staphylococcus aureus dari Swab Tangan Penjamah Makanan di Kantin Universitas Mataram Hasbi, Nurmi; Rosyunita; Rahim, Adelia Riezka; Ayunda, Rahmah Dara
Jurnal Kedokteran Universitas Palangka Raya Vol 12 No 2 (2024): Jurnal Kedokteran Universitas Palangka Raya
Publisher : Fakultas Kedokteran, Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37304/jkupr.v12i2.15313

Abstract

One of the main qualities of food is seen from the biological safety aspect, such as being free from Pathogenic microorganisms. Staphylococcus aureus is a pathogenic bacterium that can contaminate food ingredients come from the skin of food handlers. S. aureus produces enterotoxins that have a poisoning in consumers.The preliminary observationstated that there were still many food handlers in the Mataram University canteen who did not pay attention to hand hygiene and personal sanitation. This study aims to determine the bacterial contamination of S. aureus swabbed from the hands of food handlers in the Mataram University cafetaria. The method was used in this research is descriptive observational. The research consisted of two stages, namely isolation and biochemical identification. The isolation stage resulted 10 culturable bacterial isolates. All bacteria were able to ferment mannitol on Mannitol Salt Then, the biochemical test identification resulted that all isolates had a positive catalase test, a positive slide coagulase test, Gram-positive, and staphylococci cell. The TSIA test resulted A/A or (acid)/(acid) which indicated that all isolates were able to use diverse carbon sources from sucrose, lactose, glucose. Moreover, other test as sulfur, indole and motility tests were negative. Therefore, all isolates in this study are classified as S. aureus. This research is also a promotive and preventive effort for the campus in implementing hygiene and sanitation for food handlers in the cafetaria.
The Effect of Patikan Kebo (Euphorbia hirta) Herbal Extract on Collagen Levels in the Kidney and Heart of Fibrotic Rats Pahmi, Khairil; Rahim, Adelia Riezka; Musanip, Musanip; Sahrul Hudha, Lalu; Triani, Eva; Halim, Amran
Indonesian Journal of Pharmaceutical Education Vol 5, No 1 (2025): January–April 2025
Publisher : Jurusan Farmasi Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37311/ijpe.v5i1.28940

Abstract

Noncommunicable diseases (NCDs) are the leading cause of death worldwide. There are around 36 million deaths every year. An example of NCD, a non-communicable disease, is hypertension. Excessive consumption of sodium chloride (NaCl) is the main factor that can cause hypertension and organ fibrosis. A natural ingredient that has the potential to act as an antifibrosis agent against fibrosis is quercetin by increasing PPAR-γ expression. Based on research, one of the plants that contains quercetin is the herb of Patikan Kebo (Euphorbia hirta Linn). Collagen is a protein biomarker of fibrosis synthesized via the PPAR-γ signaling pathway. The aim of this research was to determine the organ protective effect of administration of Patikan Kebo herbal extract against renal fibrosis and heart in Wistar rats (Rattus norvegicus) induced with 1.5% NaCl. The method involved 15 male Wistar rats aged 2.5 months with a body weight of 100-150 grams, which were divided into three groups (KI: control; KII: induced by 1.5% NaCl; KIII: induced by 1.5% NaCl + Patikan Kebo extract 200 mg/kg body weight. The induction was carried out for 8 consecutive weeks. On day 56, the kidneys and heart were removed in groups I and II, while in group III the treatment with the Patikan-Kebo extract from Patikan-Kebo at a dose of 200 mg/kg body weight was continued for 28 days in Group III. Specifically, for group III, kidneys and heart were collected on day 84 after treatment with Patikan kebo extract on day 84. Collagen was stained with Giemsa stain. Data were analyzed using a Kruskal-Wallis test. The difference is considered significant if the probability value is p0.05. The result showed that there were significant differences between the control and treatment groups. Patikan Kebo extract can reduce collagen in rats with induced fibrosis with 1.5% NaCl.
Antibacterial Assay of Lactic Acid Bacteriea from Infant Feces on Staphylococcus aureus ATCC 25923 Raihan, Al Fikar; Rahim, Adelia Riezka; Hasbi, Nurmi
PROMOTOR Vol. 8 No. 3 (2025): JUNI
Publisher : Universitas Ibn Khaldun Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32832/pro.v8i3.1184

Abstract

The prevalence of antibiotic resistance is a serious global health problem and a threat to human health. Staphylococcus aureus is a Gram-positive bacterium that can cause various infections in humans, such as skin infections, sepsis, endocarditis, osteomyelitis, and toxins that cause food poisoning. One way to overcome the problem of antibiotic resistance is to use Lactic Acid Bacteria (LAB). LAB has many benefits, including being able to function in food fermentation, produce antimicrobial compounds, and act as probiotics. This study aims to determine the inhibitory effect of LAB from baby feces as a natural antibacterial agent against S. aureus. The method used in this study was the agar well diffusion method. The number of LAB isolates used was 8 consisting of seven cocci and two bacilli forms and also used 2 controls, namely, negative control NaCl 0.9% and Positive Control Ciprofloxacin. This study consisted of six stages including media preparation, preparation of test bacteria, sample preparation, making suspensions of S. aureus and LAB, making Mcfarland solution, and antibacterial testing using the agar well diffusion method for 3 repetitions of the treatment. The results obtained showed that all LAB isolates were able to produce an inhibition zone against Staphylococcus aureus bacteria with the results of the isolates in diameter 01A 10-5 (7.67mm), 01A 10-6(2) (6mm), 03A10-7(1) (6.67mm), 01F 10-6(2) (2mm), 01F10-7(2) (1.67mm), 02AF10-7(1) (4.33mm), 03AF10-7(2) (4mm), 04AF10-7 (3.67mm). Based on the results of the Mann-Whitney test, it was found that all LAB isolates had significant differences from the positive control Ciprofloxacin. So it can be seen that LAB from baby feces is less effective in replacing antibiotics.
Antibacterial Activity Test of Sumbawa White Honey against Escherichia coli ATCC 25922 Hulfifa, Lale Nandita; Rosyunita, Rosyunita; Wardoyo, Eustachius Hagni; Hasbi, Nurmi; Rahim, Adelia Riezka; Arnawati, Ida Ayu; Alamsyah, Abiel Dwi Cahya Firdaus; Vaidika, I Komang Satya; Hazaa, Saskia Safarina
Jurnal Biologi Tropis Vol. 24 No. 3 (2024): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i3.7407

Abstract

Due to their high mortality and morbidity rates, E. coli infections are a serious issue. Antimicrobial Resistance (AMR) is a result of this indiscriminate antibiotic use. One approach to address this issue is to utilize natural antimicrobials such as Sumbawa white honey. Sumbawa white honey contains antibacterial components like glucose and fructose (80%), water (17%), polyphenols, and hydrogen peroxide. This study aimed to analyze the antibacterial activity of white honey from Sumbawa against E. coli ATCC 25922. The research method employed was a laboratory experiment using a post test only control group design. Antibacterial testing was conducted using disc diffusion at concentrations of 25%, 75%, and 100%, with chloramphenicol as positive control and DMSO as negative control, and honey processing involved dilution. Antibacterial tests were repeated five times  and antibacterial activity was observed based on the diameter of inhibition zones around the disc. The outcomes showed that there was a restraint zone at 100 percent grouping of honey, to be specific 0.90 mm, while at 25% and 75% fixations there were no hindrance zones. According to the findings of this study, white honey from Sumbawa has a low antibacterial activity (0.90 mm), but this activity is ineffective against E. coli ATCC 25922. Further identification of the chemical components in Sumbawa white honey that are primarily responsible its antibacterial effect.
Antibacterial Activity Test of White Honey from Sumbawa NTB Againts Staphylococcus epidermidis Vaidika, I Komang Satya; Wardoyos, Eustachius Hagni; Rahim, Adelia Riezka
Jurnal Biologi Tropis Vol. 24 No. 4 (2024): Oktober - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i4.7750

Abstract

Staphylococcus epidermidis is the most common bacteria found on human skin that causes acne and is a coagulase-negative Staphylococcus species (CoNS). Resistance to methicillin is widespread, especially in Staphylococcus epidermidis bacteria or often called MRSE and is widespread globally ranging from 75-90%. Regarding antibiotic resistance, it is important to do research to find new alternatives to this problem. One of the natural antibiotics that can be used is honey which has benefits as an antibacterial because it has three systems that play a role in inhibiting bacterial growth, namely osmotic pressure, acidity and inhibine. The purpose of this study was to analyze the antibacterial effect of Sumbawa white honey on Staphylococcus epidermidis bacteria. The method used in this study was an analytical laboratory experiment with a Randomized Controlled Trial (RCT) type of post test only design. The method of making honey using disc diffusion was carried out 5 times at concentrations of 25%, 75%, 100%, positive chloramphenicol control and negative DMSO control. The results of the antibacterial test can be seen from the formation of the diameter of the inhibition zone on the disc paper. The results of the study obtained that at a concentration of 25% no inhibition zone could be formed, while in the 75% study an inhibition zone of 2.32 mm was formed and 100% of 6.71%. Based on the results of the antibacterial activity test, it can be concluded that the study on Sumbawa white honey at concentrations of 75% and 100% is included in the resistant/weak category and the activity is not effective in inhibiting the growth of S. epidermidis.
Profile and Antibiotic Assay of Cefotaxime-Resistant Lactic Acid Bacteria from The Caecum of Broiler Chickens Rosyunita, Rosyunita; Wardoyo, Eustachius Hagni; Rahim, Adelia Riezka; Nurmi , Hasbi
Jurnal Biologi Tropis Vol. 24 No. 1b (2024): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1b.7850

Abstract

A health problem associated with increasing antibiotic resistance, the silent pandemic kills 700,000 people a year, mostly in Asia and Africa. In the case of probiotic or lactic acid bacteria, antibiotic resistance can enhance nutrient absorption in the host and prevent the colonization of pathogenic bacteria. To address this, the WHO has suggested using the One Health Tricycle strategy, which entails monitoring people, the environment, and animals. One facet of this investigation is animals, specifically chickens. This study aims to identify the antibiotic resistance and profile of cefotaxime-resistant lactic acid bacteria (LAB) from broiler chicken cecum. Isolating LAB from the chicken's cecum is one of the techniques employed. After calculating the prevalence of resistant bacteria, the bacteria were characterized as macroscopic, microscopic, and biochemically. Testing the antibiotics against LAB was the next stage. According to the study's findings, the bacterial colonies were tiny to medium-sized, white, spherical, and convex, with complete margins. Gram-positive bacilli were identified by Gram staining. The findings of the biochemical tests were negative for oxidative and catalase, positive for glucose, and negative for other biochemical tests. According to antibiotic sensitivity testing, LAB was 90.9% resistant to Vancomycin, 63.63% to Chloramphenicol, and 100% to Erythromycin, Aztreonam, and Ceftriaxone. According to these findings, LAB in the chicken caecum has become resistant to several antibiotics. To give a better picture of the balance between pathogenic and helpful bacteria in the chicken cecum, more LAB testing against pathogenic bacteria is required.
Identification of Antibiotic-Resistant Gram Positive Bacteria from Broiler Caecum in The Slaughterhouse of Mataram City Rizqullah, Rifqi; Wardoyo, Eustachius Hagni; Rahim, Adelia Riezka; Rosyunita, Rosyunita; Hasbi, Nurmi; Indratama, I Nyoman Yudayana
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8466

Abstract

The subtherapeutic use of antibiotics as Antimicrobial Growth Promoters (AGPs) in broilers has accelerated Antimicrobial Drug Resistance (AMR) in gut microbiota, posing a global threat. This study aimed to analyze the population, morphology, catalase test results, and antibiotic sensitivity of erythromycin and vancomycin to cefotaxime-resistant Gram-positive bacteria in the caecum of broilers from Mataram City slaughterhouses. Using exploratory descriptive method, five caecum samples were analyzed by Total Plate Count (TPC) on Man Rogosa Sharpe Agar (MRSA) media with and without cefotaxime, and incubated on Mannitol Salt Agar (MSA). Results revealed uniform bacterial morphology on MRSA (small, round, convex, entire edge, white, Gram-positive colonies) but varied morphologies on MSA. Catalase tests were negative on MRSA but mixed on MSA. Resistance to erythromycin and vancomycin was 80% on MRSA, while on MSA, erythromycin resistance reached 62.5% with variable vancomycin inhibition zones. The prevalence of cefotaxime-resistant bacteria was 5.24%. This study highlights diverse morphological, catalase, and antibiotic sensitivity profiles in cefotaxime-resistant bacteria, particularly on MSA. These findings underscore the need for stricter antibiotic use regulations and further research to mitigate AMR spread in poultry production.
The Relathionship Between CD31 Immunohistochemical Expression and Meningioma Grading Differences Febriana, Nanggi Qoriatul; Rosyidi, Rohadi Muhammad; Januarman, Januarman; Rahim, Adelia Riezka; Prihatina, Lale Maulin
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8539

Abstract

The proper management of meningioma patients requires a definitive diagnosis of the meningioma grade by examining the expression of CD31 in tumor blood vessels using immunohistochemical staining. This study aims to determine the relationship between CD31 immunohistochemical expression and the grading differences of meningiomas. Nine paraffin block samples from the surgical tissue of meningioma patients were used, with three samples each from grade I, grade II, and grade III meningiomas. Immunohistochemical staining for CD31 was then performed on each meningioma slide, and the samples were observed under a binocular light microscope with 200x magnification. The results showed CD31 expression in grade I as 90%, 40%, and 80%; in grade II as 80%, 80%, and 60%; and in grade III as 40%, 20%, and negative (0%). The statistical test results of this study indicate a strong negative correlation between CD31 immunohistochemical expression and meningioma grading differences. The higher the meningioma grade, the lower the CD31 expression found, and vice versa. This research is important to assist neurosurgeons in the proper management of meningioma patients, potentially preventing poor prognosis and complications. It is hoped that future studies will analyze the relationship between CD31 immunohistochemical expression with subtypes of each meningioma grade and their respective locations.