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All Journal HAYATI Journal of Biosciences AL KAUNIYAH Jurnal Biologi Universitas Andalas JURNAL PENGABDIAN KEPADA MASYARAKAT Journal of Tropical Biodiversity and Biotechnology Jurnal Dinamika Pengabdian (JDP) Medical Laboratory Technology Journal Jurnal Biologi Tropis JSFK (Jurnal Sains Farmasi & Klinis) Jurnal Hilirisasi IPTEKS (JHI) Jurnal Pengabdian kepada Masyarakat Nusantara Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks Journal of Tropical Pharmacy and Chemistry
Putra Santoso
Universitas Andalas
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Analisis Histologis Ginjal Ikan Asang (Osteochilus hasseltii ) di Danau Maninjau dan Singkarak, Sumatera Barat Sayati Mandia; Netti Marusin; Putra Santoso
Jurnal Biologi Universitas Andalas Vol 2, No 3 (2013)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.2.3.%p.2013

Abstract

A study on kidney histology of Silver Sharkminnow (Osteochilus hassletii) sampled from Maninjau and Singkarak Lakes, West Sumatra has been done from December 2012 to April 2013 at Animal Structure and Development Laboratory, Department of Biology, Andalas University, Padang. Direct observation and purposive sampling were conducted at four locations around Singkarak Lake (Sumani, Paninggahan, Sumpur, Ombilin) and one location in Maninjau Lake (Tanjung Sani). Histological samples of kidneys were isolated, fixated, dehydrated and embedded on paraffin and stained using Haematoxylin-Eosin. The results showed some histological alterations of kidney samples from all locations. Those alteration varied from cell hypertrophy, lysis, necrosis and emerging of scrape tissues. There were no significant differences on the degree of alteration among all populations. Those result indicated that both lakes had been experienced organic pollutions.Keywords: Silver Sharkminnow (Osteochilus hasseltii C.V), Histopatology, Kidney, Singkarak Lake, Maninjau Lake.
Laju Respirasi Kecoak Jerman (Blattella germanica, Dictyoptera; Blattellidae) yang Resisten Terhadap Insektisida Robby Jannatan; Resti Rahayu; Putra Santoso
Jurnal Biologi Universitas Andalas Vol 2, No 4 (2013)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.2.4.%p.2013

Abstract

An experiment on respiration rate of German cockroach (Blattella germanica, Dictyoptera; Blattellidae) that had been resistant to pyrethroid insecticide was done from December 2012 to Mei 2013. This experiment used completely randomized design with two treatments (with insecticide and without insecticide exposured) and three replications. The respiration rate were measured using the Scholander Respirometer. The control subject were VCRU-WHO as a susceptible strain standarized by WHO, while the resistance strains were GFA-JKT, KRSA-BDG, PLZ-SMRD and PLZ-PDG consisted of female, male and nymph Individuals. The results shown that respiration rates were not significant difference between susceptible and resistance strains on females (F: 0.373, df: 4, P: 0.823), males (F: 0.562, df: 4, P: 0.696) and nymphs (F: 0.402, df: 4, P: 0.803). The respiration rate were also not significant difference between with insecticide and without insecticide exposured, except within GFA-JKT females (F: 9.161, df: 4, P: 0.039), VCRU-WHO males (F: 11.779, df: 4, P: 0.026) and GFA-JKT nymphs (F: 8.733, df: 4, P: 0.042). Those results support that resistant cockroach do not increase respiration rate in responding insecticide exposured. Keywords: German cockroach, respiration rate, strains, insecticide exposured
Struktur Histologis Insang dan Kadar Hemoglobin Ikan Asang (Osteochilus hasseltii C.V) di Danau Singkarak dan Maninjau, Sumatera Barat Hari Marta Saputra; Netti Marusin; Putra Santoso
Jurnal Biologi Universitas Andalas Vol 2, No 2 (2013)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.2.2.%p.2013

Abstract

A study on histological structure of gill and hemoglobin concentration of Silver Sharkminnow (Osteochilus hassletii C.V) in Singkarak and Maninjau Lakes, West Sumatera has been conducted from December 2012 to March 2013. This study aimed to determine the damage of histological structure of gill and haemoglobin concentration of O. hasseltii. We prepared slide preparation of gills to observe their damage structure, while hemoglobin concentration was counted/measured using Sahli method. The results showed various types of damage such as edema, loss of the epithelium on the secondary lamella, hyperplasia, fusion of secondary lamella, the loss of the secondary lamella structure, clubbing and thickening of elastic cartilage. Hemoglobin concentration of O. hasseltii ware 3.28% and 3.05 % for Singkarak and Maninjau respectively. There was no statistically significant difference between sampling sites for both of studied parameters.Keywords: Ostechilius hasseltii, gill histology, hemoglobin, Singkarak and Maninjau lakes
PEMBERDAYAAN MASYARAKAT MELALUI PEMANFAATAN LIMBAH ORGANIK PADA BUDIDAYA BAWANG MERAH DI NAGARI GUNUANG RAJO Yulmira Yanti; Hasmiandy Hamid; Putra Santoso
Jurnal Hilirisasi IPTEKS Vol 2 No 3.a (2019)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jhi.v2i3.a.237

Abstract

Program pengabdian masyarakat ini dilatarbelakangi ketakpahaman masyarakat tentang manfaat dari limbah-limbah pertanian yang ada di sekitar Nagari Gunung Rajo. Limbah dari kegiatan bertani dan beternak tidak ter manfaatkan dengan baik, padahal limbah tersebut dapat digunakan kembali oleh masyarakat. Salah satu teknologi sederhana tepat guna yang dapat dilakukan adalah dengan memanfaatkan limbah dan teknologi rizobakteri sebagai pupuk organik. Salah satu jenis pupuk organik yang terbaik adalah berupa kompos, karena dapat menyediakan unsur hara bagi tanaman dan memperbaiki struktur maupun kesuburan tanah. Adapun bahan-bahan yang digunakan dalam pembuatan rizokompos adalah tumbuhan Tithonia diversifolia, sekam padi, dedak dan pupuk kandang sapi. Dalam proses dekomposisi digunakan Rizobakteri sebagai decomposer dengan cara rizobakteri disiramkan pada bahan kompos, lalu kompos dibalik dan diberi rizobakteri kembali setiap minggu dengan proses pembuatan total 3 minggu. Program ini bertujuan untuk meningkatkan pengetahuan masyarakat tentang pemanfaatan limbah pertanian dan aplikasi rizobakteri indigenos dalam pembuatan kompos. Kegiatan ini telah dilakukan dalam bentuk penyuluhan dan demonstrasi. Kegiatan ini sangat bermanfaat bagi masyarakat khususnya masyarakat Nagari Gunung Rajo. Antusias dan partisipasi aktif dari masyarakat sangat menunjang keberhasilan dari kegiatan ini. Pertumbuhan dan hasil produksi yang terbaik didapatkan pada perlakuan rizokompos dan rizobakteri dibanding diaplikasikan secara tunggal.
Pelatihan Teknik Budidaya Mencit Putih (Mus musculus L.) sebagai Peluang Usaha Kreatif kepada Siswa SMAN 5 Bukittinggi Putra Santoso; Resti Rahayu; Robby Jannatan
Warta Pengabdian Andalas Vol 27 No 2 (2020)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jwa.27.2.104-112.2020

Abstract

A marked increase in demand for white mice (Mus musclulus L., mammals) in the Indonesian market has been attributed to the price soar, while the availability of mice with a standardized quality remains very limited. Considering such condition, a business focusing on standardized farm of white mice should be actively socialized to younger people thereby elevating their enthusiasm in the entrepreneurship. This community service program aimed to provide insights as well as to train the basic skills in white mouse farming for students of the National High School Number 5 Bukittinggi. The program has been carried out for one day in the Biology Department, Andalas University with 45 participants. We deployed the lectures and hands-on practice on the aspects of white mouse farming. All participants have directly practiced various principle techniques in mice farming such as handling, sexing, mating, and raising brooders. This activity has increased the interest of the participants in such creative business. In the future, some efforts should be made to ensure the sustainability of the program by involving the participants from other schools, teachers and unemployed youth in West Sumatra.
In Silico Study on Anti-inflammatory Effect of Bioactive Compounds of Velvet Bean (Mucuna pruriens) Leaves Against NF-kB Activation Pathway Fadilaturahmah Fadilaturahmah; Resti Rahayu; Putra Santoso
Jurnal Sains Farmasi & Klinis Vol 9 (2022): J Sains Farm Klin 9(suplemen), Desember 2022
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.9.sup.168-174.2022

Abstract

Activation of nuclear factor-kB (NF-ĸB) receptors potently enhances pro-inflammatory cytokines production thereby promoting inflammatory reactions. NF-ĸB receptor inhibition is one of the targets in overcoming inflammatory reactions. One of the natural ingredients that have the potential as an anti-inflammatory is velvet bean leaf extract (Mucuna pruriens L. (DC.)) which is used by the local people of West Sumatra. This study aimed to investigate the anti-inflammatory mechanism of the bioactive compounds detected in velvet bean leaf extract against NF-ĸB (1U36) activation pathway using an in silico approach. The phytochemical analysis of the ethanolic extract of velvet bean leaves was performed using GC-MS. Subsequently, their potential bioactivities were explored using PASS online test, preparation of ligands, receptors, and molecular docking. The results showed that the 34 compounds contained in the miang bean leaf extract had anti-inflammatory bioactivity with a probability activity value of >0.7 based on PASS online. The docking results showed that the highest binding affinity value was found in artemin (-7.5 kcal/mol) which was higher than the standard anti-inflammatory drug ketoconazole (-7.2 kcal/mol). Therefore, one of the mechanisms of miang bean leaves as an anti-inflammatory is due to the inhibitory action of artemin on the NF-kB activation pathway. 
Efektivitas Ekstrak Biji Teratai (Nymphaea pubescens Willd) dalam Meningkatkan Perilaku Neurokognitif pada Mencit yang Diinduksi Trimetiltin Dinda Fadhilah Belahusna; Putra Santoso; Resti Rahayu
Jurnal Sains Farmasi & Klinis Vol 9 (2022): J Sains Farm Klin 9(suplemen), Desember 2022
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.9.sup.152-159.2022

Abstract

Neurodegenerasi adalah penyakit yang mengakibatkan degenerasi progresif dan kematian sel saraf di otak. Penelitian ini bertujuan untuk mengungkap khasiat neuroprotektif ekstrak biji teratai (Nymphaea pubescens Willd) melalui analisis perilaku neurokognitif. Sebanyak 25 ekor mencit jantan dibagi secara acak menjadi 5 perlakuan: kontrol negatif (Na-CMC 0,5%), kontrol positif diinduksi trimetiltin (TMT) 0,6mg/kgBB, dan pemberian ekstrak biji teratai (100, 200, dan 400 mg/kgBB). Induksi degenerasi otak dengan TMT menggunakan dosis tunggal secara intraperitoneal. Ekstrak diberikan tiga hari pasca injeksi TMT secara oral setiap hari selama 28 hari. Pada akhir perlakuan, tes neurobehavioral dilakukan meliputi uji kecerdasan memori dengan Hebb-Williams Maze, uji keingintahuan dengan Hole-Board dan uji interaksi sosial. Selanjutnya dilakukan pengukuran indeks organ otak. Data dianalisis secara statistik dengan One-Way Anova dilanjutkan dengan uji DNMRT (P<0,05). Hasil penelitian menunjukkan bahwa ekstrak biji teratai dosis 200 dan 400 mg/kgBB secara signifikan dapat meningkatkan kecerdasan kognitif (memori, keingintahuan dan interaksi sosial) pada mencit yang diinduksi TMT, namun tidak berpengaruh signifikan terhadap indeks bobot otak. Temuan penelitian ini mengungkapkan bahwa biji teratai adalah kandidat kuat untuk sumber obat anti-neurodegeneratif.
In Silico Analysis of Gene Expression Location and Single Nucleotide Polymorphisms (SNPs) of The Glucagon Like Peptide 1 Receptor (GLP-1R) Rita Maliza; Putra Santoso; Bramadi Arya; Alimuddin Tofrizal; Rarastoeti Pratiwi
HAYATI Journal of Biosciences Vol. 30 No. 2 (2023): March 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.2.281-291

Abstract

Association studies of SNPs have become very important in determining how genetic variants are linked to complex diseases, quantitative traits, and physiological responses. Genetic polymorphisms in the GLP-1R gene have potentially decreased protein stability and are associated with many diseases, especially diabetes and obesity. This study aimed to screen the expression and investigate the genetic polymorphism of the GLP-1R by using several beneficial bioinformatics tools. We observed database Ensembl, GTEx portal, and KEGG to identify the structure, expression, and molecular pathway of GLP-1R. In silico computational methods (SIFT, PolyPhen v2, PROVEAN, and PhD-SNP) were used to identify nsSNPs of the GLP-1R that potentially influence protein structure and function. I-Mutant was used to investigate possibly damaging nsSNPs, while GeneMANIA was used to investigate GLP-1R gene-gene interactions. GLP-1R is localized on chromosome 6p21, contains 13 exons, and has the regulation variant (CTCF, promotor, enhancer, and promotor flank region). GLP-1R is highly expressed in the pancreas to stimulate glucose-dependent insulin secretion and suppress glucagon secretion. Seven nsSNPs of the GLP-1R gene were found to be potentially deleterious: rs10305421, rs201672448, rs10305492, rs2295006, rs6923761, rs1042044, rs140642887, and rs10305510. I-Mutant server showed that nsSNPs rs140642887 was unstable, decreased GLP-1R protein stability, and impaired other genes' interaction and function (SP1, SP3, GNAS, and GCG). This study is the first in silico analysis of the polymorphic GLP-1R gene, and will serve as a great resource for developing precision medications to treat diseases associated with these polymorphisms.
PELATIHAN PEMBUATAN KAIN ECOPRINT BAGI KELOMPOK PKK KAMPUNG WISATA BUKIT NOBITA, SUMATRA BARAT Rita Maliza; Tofrizal Tofrizal; Muhammad Nazri Janra; Robby Jannatan; Putra Santoso; Muhammad Idris; Nofrita Nofrita; Fadillah Fadillah
JURNAL PENGABDIAN KEPADA MASYARAKAT Vol 29, No 2 (2023): APRIL-JUNI
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkm.v29i2.40929

Abstract

Kain ecoprint pada saat sekarang mengalami masa populer dan memiliki harga ekonomis yang tinggi, bahkan sampai menembus pasar internasional. Pembuatan kain ecoprint dengan mengembangkan keterampilan kain kreatif yang melibatkan pewarna alami dari alam seperti daun, ranting dan bunga merupakan salah satu produk yang mencerminkan karya yang kreatif dan berkarakter cinta lingkungan (ecofriendly). Kegiatan pengabdian ini bertujuan untuk meningkatkan keterampilan dan wawasan Ibu-Ibu PKK kampung wisata Bukit Nobita, Kelurahan Kampung Jua, Padang, dalam memanfaatkan potensi lingkungan yang ada disekitarnya untuk menghasilkan suatu produk/barang yang memiliki potensi ekonomis dan dijadikan salah satu produk kreatif dari kampung wisata bukit nobita nantinya. Metode pelaksanaan pengabdian meliputi beberapa tahap yaitu tahap observasi dan koordinasi, tahap persiapan, tahap pelaksanaan yang terdiri dari empat kegiatan dan tahap evaluasi. Hasil yang didapatkan dari pelatihan, Mitra telah bisa membuat kain batik ecoprint dan sudah memahami tahapan-tahapan pembuatan kain ecoprint. Kegiatan pengabdian ini meningkatkan keberdayaan mitra pada pengetahuan dan keterampilan, kreatifitas yang nantinya bisa digunakan untuk menjadi salah satu usaha atau kegiatan Ibu-Ibu PKK di kampung wisata Bukit Nobita.
Pengembangan Strategi Promosi Madu Galo-galo UMKM Usaha Bukik Nabu (UBUNA) Henny Herwina; Jasmi Jasmi; Eli Ratni; Wellyalina Wellyalina; Elni Fatimah; Nadira Nurul Fathiyah; Maya Sari; Feskaharni Alamsjah; Putra Santoso
Warta Pengabdian Andalas Vol 30 No 2 (2023)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jwa.30.2.253-260.2023

Abstract

Stingless bee cultivation in Indonesia, one of which is in West Sumatra, has the potential as an effort to improve the community's economy. Stingless bees, commonly called Galo-galo, can produce honey, propolis, and bee pollen, which have many benefits and produce high selling power. The community service activity accompanying the Bukik Nabu Business (UBUNA) aimed to develop the Galo-galo cultivation business in the Limau Manis village. This village has optimum natural conditions to cultivate stingless bees due to the availability of feed plant surroundings. The method used directly assisted the development of Micro, Small and Medium Enterprises of UBUNA. It started by adding the number of colonies to the community, harvesting independently on the type of Heterotrigona itama and Geniotrigona thoracica, upscaling branding, making product labels, product promotions, legality, and participating in collaborative events and festivals.
Co-Authors . Mansyurdin Adelia, sabbrina Adilla, Ardis Trianita Afriani, Nita Afriani, Yona Aini, Wardatul Alimuddin Tofrizal Asful, Ferdhinal Ashrifurrahman, Ashrifurrahman Asih, Enda Tarni Aszareta, Muhammad Andoni Aziz, M. Abdul Belahusna, Dinda Fadhilah Bramadi Arya Dahelmi Dahelmi Delfi, Shyla Aulia Dinda Fadhilah Belahusna Efendi, Rilwan Efrizal Efrizal Eli Ratni Elni Fatimah Elni Fatimah Eti Yerizel Fadhilah, Diana Fadilaturahmah Fadilaturahmah Fadillah Fadillah Fadillah Fajri Hidayat Feskaharni Alamsjah Firman Syukri Hari Marta Saputra Hasmiandy Hamid Henny Herwina Hermeria, Noveriza Husna Yetti Jabang Nurdin Janra, Muhammad Nazri Jasmi Jasmi Jasmi Jefrial Santoso Maliza, Rita Maya Sari Miftahul Ilmi Muhamma Idris Muhammad Afif Muhammad Hamdi Ibrahim Muhammad Idris Muhammad Idris Muhammad Idris Muhammad Ilham Samudra Muhammad Nazri Janra Muhammad Nazri Janra Nabilah Rahmachila Azura Nadira Nurul Fathiyah Nadjmir, Mikail Netti Marusin Netti Marusin Nofrita Nofrita Nofrita Nofrita Nurhafitri, Amanda Pasha, Gusti Ari Afrilya Pratama Elisa, Tasya Putri Pratama, Raihan Anugrah Puspita, Ayumi Rizci Puti Khairunnajwa Amar Putri, Mellanie Alia Putri, Suci Indah Putri, Syntia Mai Rarastoeti Pratiwi Rauza Sukma Rita Resti Rahayu Resti Rahayu Reziq Marchellino Irwan Ridho, Muhammad Syifa'ur Rilwan Efendi Robby Jannatan Rusiati, Anisa Rahman Sayati Mandia Siagian, Marhamah Sinurat, Arthauly Yopita Syabila, Hutri Dinda Syaifullah Syaifullah Syakira Tiara Rezvi Syamsuardi Syamsuardi Tasya Putri Pratama Elisa Tofrizal Tofrizal, Alimuddin Wardahtul Aini Wellyalina, Wellyalina Wilson Novarino Yona Afriani Yulmira Yanti Zaini Zaini Zikrah, Azizah Amatu Zozy Aneloi Noli Zulkarnain, Alivia