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PELATIHAN IMPLEMENTASI SANITASI DAN HIGIENE PADA PROSES PENGOLAHAN IKAN ASAP KENJERAN Pramono, Heru; Andriyono, Sapto; Wisudyawati, Dita; Sari, Alfi Hermawati Waskita; Hasan, Veryl; Hidayati, Nuning Vita
Balobe: Jurnal Pengabdian Masyarakat Vol 3 No 1 (2024): Balobe: Jurnal Pengabdian Masyarakat
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/balobe.3.1.20-29

Penerapan higiene dan sanitasi merupakan aspek penting dalam keamanan pangan, termasuk pada pengolahan ikan asap. Kesadaran para pengolah ikan asap akan pentingnya keamanan pangan serta tata cara penerapan higiene dan sanitasi sangat diperlukan. Artikel ini melaporkan proses sosialisasi penerapan higiene dan sanitasi pada pengolahan ikan asap di Kenjeran, Surabaya, Indonesia. Metode pelaksanaan program dimulai dengan brainstorming dengan aparat pemerintah setempat, dilanjutkan dengan pelatihan internasional oleh pembicara Malaysia dan Indonesia, praktik penggunaan peralatan keselamatan, kelas memasak, dan expo. Dari diskusi awal terlihat jelas bahwa masyarakat memahami pentingnya penerapan higiene dan sanitasi, namun masih membutuhkan informasi mengenai peningkatan ukuran pasar (market size). Selama pelatihan internasional, pembicara dari Malaysia dan Indonesia membahas korelasi pemasaran online dan higiene dan sanitasi serta strategi memasak alternatif yang kemudian diimplementasikan pada expo. Produk siap santap (RTE) yang dihasilkan oleh masyarakat potensial untuk dipasarkan lebih lanjut melalui pendekatan online, namun aspek pengemasan dan pemasaran masih perlu dikaji lebih lanjut. Dari penelitian ini, penerapan higiene dan sanitasi di fasilitas pengolahan ikan asap mungkin efektif jika dipadukan dengan cara memasak dan pemasaran alternatif.

PENDAMPINGAN MANAJEMEN KUALITAS AIR DAN APLIKASI PROBIOTIK PADA BUDIDAYA IKAN LELE PONDOK DARUL MUKMIN, SUKOREJO, PASURUAN JAWA TIMUR Nina Nurmalia Dewi; Woro Hastuti Satyantini; Wahyu Isroni; Gunanti Mahasri; Abdul Manan; Yudi Cahyoko; Dita Wisudyawati
RAMBIDEUN : Jurnal Pengabdian Kepada Masyarakat Vol. 7 No. 3 (2024): Rambideun: Jurnal Pengabdian Kepada Masyarakat
Publisher : LPPM Universitas Al Muslim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51179/pkm.v7i3.2955

Pasuruan Regency is one of the regencies that has potential in the fisheries sector, especially freshwater fisheries cultivation. The potential area for cultivation activities is located in Sukorejo District with the cultivated commodity being catfish. One of the farmer groups in Sukorejo District is the Pondok Darul Mukmin Sangkuriang Catfish farmer. However, this catfish cultivation activity has not been running optimally, because the cultivation system used is still traditional. This community service activity aimed to provide assistance related to water quality management and probiotic application in catfish cultivation. This activity was carried out at Pondok Darul Mukmin from July to September 2024. The method of implementing the activity was in the form of training, assistance, and demonstrations of water quality management, probiotic application in feed, and probiotic culture for catfish cultivation. The results of this community service activity showed that Pondok Darul Mukmin fish farmer were very enthusiastic in participating in the training activities. In addition, the community can also carry out catfish cultivation by monitoring water quality and applying probiotics to feed. Cultivation activities resulted in increased catfish growth every week.

PENDAMPINGAN MANAJEMEN KUALITAS AIR DAN APLIKASI PROBIOTIK PADA BUDIDAYA IKAN LELE PONDOK DARUL MUKMIN, SUKOREJO, PASURUAN JAWA TIMUR Dewi, Nina Nurmalia; Satyantini, Woro Hastuti; Isroni, Wahyu; Mahasri, Gunanti; Manan, Abdul; Cahyoko, Yudi; Wisudyawati, Dita
RAMBIDEUN : Jurnal Pengabdian Kepada Masyarakat Vol. 7 No. 3 (2024): Rambideun: Jurnal Pengabdian Kepada Masyarakat
Publisher : LPPM Universitas Al Muslim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51179/pkm.v7i3.2955

Pasuruan Regency is one of the regencies that has potential in the fisheries sector, especially freshwater fisheries cultivation. The potential area for cultivation activities is located in Sukorejo District with the cultivated commodity being catfish. One of the farmer groups in Sukorejo District is the Pondok Darul Mukmin Sangkuriang Catfish farmer. However, this catfish cultivation activity has not been running optimally, because the cultivation system used is still traditional. This community service activity aimed to provide assistance related to water quality management and probiotic application in catfish cultivation. This activity was carried out at Pondok Darul Mukmin from July to September 2024. The method of implementing the activity was in the form of training, assistance, and demonstrations of water quality management, probiotic application in feed, and probiotic culture for catfish cultivation. The results of this community service activity showed that Pondok Darul Mukmin fish farmer were very enthusiastic in participating in the training activities. In addition, the community can also carry out catfish cultivation by monitoring water quality and applying probiotics to feed. Cultivation activities resulted in increased catfish growth every week.

Polyvalent formalin-killed Aeromonas hydrophila vaccine with oil adjuvant drives coordinated humoral, innate, and cytokine responses in giant gourami (Osphronemus goramy) Rozi; Wiwiek Tyasningsih; Jola Rahmahani; Eduardus Bimo Aksono; Muchammad Yunus; Mohammad Anam Al-Arif; Suryo Kuncorojakti; Daruti Dinda Nindarwi; Putri Desi Wulan Sari; Nina Nurmalia Dewi; Woro Hastuti Satyantini; Muhammad Browijoyo Santanumurti; Dita Wisudyawati; Mohammad Noor Amal Azmai; Annas Salleh; Gazali Salim; Suwarno, Suwarno
Jurnal Ilmiah Perikanan dan Kelautan 2026: JIPK VOLUME 18 ISSUE 1 YEAR 2026 (FEBRUARY 2026, ISSUE IN PROGRESS)
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar

Graphical Abstract Highlight Research Adjuvanted polyvalent FKC elicited the strongest and most sustained multi-arm immune response in Osphronemus goramy compared with monovalent and non-adjuvanted vaccines. The lead formulation combined high agglutinating antibody titres with enhanced NBT respiratory burst, indicating synergistic humoral–innate activation against Aeromonas hydrophila. Polyvalent vaccines did not dilute immunogenicity; instead, strain combination plus adjuvant broadened and amplified immune responsiveness. Longitudinal profiling of il-1β and ifn-γ revealed a stable pro-inflammatory/Th1-like cytokine signature uniquely associated with the adjuvanted polyvalent FKC. The integrated immunological “fingerprint” supports the adjuvanted polyvalent FKC as a rational lead candidate for motile Aeromonas septicaemia control in warm-water gourami aquaculture. Abstract Bacterial septicaemia caused by Aeromonas hydrophila is an important constraint for giant gourami (Osphronemus goramy) culture in Southeast Asia. Inactivated whole-cell (bacterin) vaccines are widely used against bacterial diseases in aquaculture, but comparative data on monovalent versus polyvalent A. hydrophila vaccines, with and without oil-based adjuvant, remain scarce for this species. This study evaluated the safety, immunological responses, and protective efficacy of three formalin-killed cell (FKC) vaccines prepared from gourami-derived A. hydrophila isolates: a monovalent FKC (P2), a non-adjuvanted polyvalent FKC (P3), and an oil-adjuvanted polyvalent FKC (P4), using PBS (P1) as a control. Sub-adult giant gourami were vaccinated intraperitoneally and monitored for 42 days. Serum agglutinating antibody titres, nitroblue tetrazolium (NBT)-reducing activity, and splenic il-1β and ifn-γ mRNA expression were measured at multiple time points. At 21 days post-vaccination, a separate cohort was challenged intraperitoneally with virulent A. hydrophila Ah-S1, and survival was recorded for 14 days; relative percent survival (RPS) was calculated at day 14. All FKC formulations were clinically well tolerated, with only transient post-vaccination inappetence and no gross injection-site pathology. Vaccination induced clear, treatment- and time-dependent increases in agglutinating antibody titres, NBT-reducing activity, and splenic il-1β and ifn-γ transcription, with the strongest and most sustained responses in P4, intermediate responses in P3 and P2, and minimal changes in P1. Following homologous challenge, day-14 survival was 8.3% in P1, 61.7% in P2, 75.0% in P3, and 83.3% in P4, with corresponding RPS values of 58.2%, 72.7%, and 81.8%, respectively. Under these experimental conditions, the oil-adjuvanted polyvalent FKC (P4) produced the greatest enhancement of immune responses and protection against intraperitoneal A. hydrophila challenge in giant gourami. These findings support this formulation as a candidate for further vaccine development in O. goramy and highlight the need for dose optimisation, safety assessment, heterologous challenge, and field-validation studies before recommendations for large-scale use in aquaculture.

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