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PELATIHAN IMPLEMENTASI SANITASI DAN HIGIENE PADA PROSES PENGOLAHAN IKAN ASAP KENJERAN Pramono, Heru; Andriyono, Sapto; Wisudyawati, Dita; Sari, Alfi Hermawati Waskita; Hasan, Veryl; Hidayati, Nuning Vita
Balobe: Jurnal Pengabdian Masyarakat Vol 3 No 1 (2024): Balobe: Jurnal Pengabdian Masyarakat
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/balobe.3.1.20-29

Penerapan higiene dan sanitasi merupakan aspek penting dalam keamanan pangan, termasuk pada pengolahan ikan asap. Kesadaran para pengolah ikan asap akan pentingnya keamanan pangan serta tata cara penerapan higiene dan sanitasi sangat diperlukan. Artikel ini melaporkan proses sosialisasi penerapan higiene dan sanitasi pada pengolahan ikan asap di Kenjeran, Surabaya, Indonesia. Metode pelaksanaan program dimulai dengan brainstorming dengan aparat pemerintah setempat, dilanjutkan dengan pelatihan internasional oleh pembicara Malaysia dan Indonesia, praktik penggunaan peralatan keselamatan, kelas memasak, dan expo. Dari diskusi awal terlihat jelas bahwa masyarakat memahami pentingnya penerapan higiene dan sanitasi, namun masih membutuhkan informasi mengenai peningkatan ukuran pasar (market size). Selama pelatihan internasional, pembicara dari Malaysia dan Indonesia membahas korelasi pemasaran online dan higiene dan sanitasi serta strategi memasak alternatif yang kemudian diimplementasikan pada expo. Produk siap santap (RTE) yang dihasilkan oleh masyarakat potensial untuk dipasarkan lebih lanjut melalui pendekatan online, namun aspek pengemasan dan pemasaran masih perlu dikaji lebih lanjut. Dari penelitian ini, penerapan higiene dan sanitasi di fasilitas pengolahan ikan asap mungkin efektif jika dipadukan dengan cara memasak dan pemasaran alternatif.

PENDAMPINGAN MANAJEMEN KUALITAS AIR DAN APLIKASI PROBIOTIK PADA BUDIDAYA IKAN LELE PONDOK DARUL MUKMIN, SUKOREJO, PASURUAN JAWA TIMUR Nina Nurmalia Dewi; Woro Hastuti Satyantini; Wahyu Isroni; Gunanti Mahasri; Abdul Manan; Yudi Cahyoko; Dita Wisudyawati
RAMBIDEUN : Jurnal Pengabdian Kepada Masyarakat Vol. 7 No. 3 (2024): Rambideun: Jurnal Pengabdian Kepada Masyarakat
Publisher : LPPM Universitas Al Muslim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51179/pkm.v7i3.2955

Pasuruan Regency is one of the regencies that has potential in the fisheries sector, especially freshwater fisheries cultivation. The potential area for cultivation activities is located in Sukorejo District with the cultivated commodity being catfish. One of the farmer groups in Sukorejo District is the Pondok Darul Mukmin Sangkuriang Catfish farmer. However, this catfish cultivation activity has not been running optimally, because the cultivation system used is still traditional. This community service activity aimed to provide assistance related to water quality management and probiotic application in catfish cultivation. This activity was carried out at Pondok Darul Mukmin from July to September 2024. The method of implementing the activity was in the form of training, assistance, and demonstrations of water quality management, probiotic application in feed, and probiotic culture for catfish cultivation. The results of this community service activity showed that Pondok Darul Mukmin fish farmer were very enthusiastic in participating in the training activities. In addition, the community can also carry out catfish cultivation by monitoring water quality and applying probiotics to feed. Cultivation activities resulted in increased catfish growth every week.

PENDAMPINGAN MANAJEMEN KUALITAS AIR DAN APLIKASI PROBIOTIK PADA BUDIDAYA IKAN LELE PONDOK DARUL MUKMIN, SUKOREJO, PASURUAN JAWA TIMUR Dewi, Nina Nurmalia; Satyantini, Woro Hastuti; Isroni, Wahyu; Mahasri, Gunanti; Manan, Abdul; Cahyoko, Yudi; Wisudyawati, Dita
RAMBIDEUN : Jurnal Pengabdian Kepada Masyarakat Vol. 7 No. 3 (2024): Rambideun: Jurnal Pengabdian Kepada Masyarakat
Publisher : LPPM Universitas Al Muslim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51179/pkm.v7i3.2955

Pasuruan Regency is one of the regencies that has potential in the fisheries sector, especially freshwater fisheries cultivation. The potential area for cultivation activities is located in Sukorejo District with the cultivated commodity being catfish. One of the farmer groups in Sukorejo District is the Pondok Darul Mukmin Sangkuriang Catfish farmer. However, this catfish cultivation activity has not been running optimally, because the cultivation system used is still traditional. This community service activity aimed to provide assistance related to water quality management and probiotic application in catfish cultivation. This activity was carried out at Pondok Darul Mukmin from July to September 2024. The method of implementing the activity was in the form of training, assistance, and demonstrations of water quality management, probiotic application in feed, and probiotic culture for catfish cultivation. The results of this community service activity showed that Pondok Darul Mukmin fish farmer were very enthusiastic in participating in the training activities. In addition, the community can also carry out catfish cultivation by monitoring water quality and applying probiotics to feed. Cultivation activities resulted in increased catfish growth every week.

Oil-Adjuvanted Polyvalent Formalin-killed Aeromonas hydrophila Vaccine Enhances Agglutinating Antibodies, Respiratory Burst, and Survival in Giant Gourami Rozi; Wiwiek Tyasningsih; Jola Rahmahani; Eduardus Bimo Aksono; Muchammad Yunus; Mohammad Anam Al-Arif; Suryo Kuncorojakti; Daruti Dinda Nindarwi; Putri Desi Wulan Sari; Nina Nurmalia Dewi; Woro Hastuti Satyantini; Muhammad Browijoyo Santanumurti; Dita Wisudyawati; Mohammad Noor Amal Azmai; Annas Salleh; Gazali Salim; Suwarno, Suwarno
Jurnal Ilmiah Perikanan dan Kelautan Vol. 18 No. 1 (2026): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v18i1.82866

Graphical Abstract Highlight Research Adjuvanted polyvalent FKC elicited the strongest and most sustained multi-arm immune response in Osphronemus goramy compared with monovalent and non-adjuvanted vaccines. The lead formulation combined high agglutinating antibody titres with enhanced NBT respiratory burst, indicating synergistic humoral–innate activation against Aeromonas hydrophila. Polyvalent vaccines did not dilute immunogenicity; instead, strain combination plus adjuvant broadened and amplified immune responsiveness. Longitudinal profiling of il-1β and ifn-γ revealed a stable pro-inflammatory/Th1-like cytokine signature uniquely associated with the adjuvanted polyvalent FKC. The integrated immunological “fingerprint” supports the adjuvanted polyvalent FKC as a rational lead candidate for motile Aeromonas septicaemia control in warm-water gourami aquaculture. Abstract Motile Aeromonas septicaemia (MAS), predominantly associated with Aeromonas hydrophila, remains a major constraint in giant gourami (Osphronemus goramy) aquaculture. This study evaluated formalin-inactivated A. hydrophila vaccines prepared from MAS-associated field isolates, comparing a monovalent formulation (P2), a non-adjuvanted polyvalent formulation (P3), and an oil-adjuvanted polyvalent formulation (P4) against PBS controls (P1). A total of 240 fish were used (60 per treatment) and assigned to two parallel cohorts (immunology and survival/challenge). Immune endpoints (agglutinating titres, NBT activity, and splenic il-1β and ifn-γ transcription) were assessed on days 7, 14, 21, 35, and 42 post-vaccination. The survival cohort was challenged intraperitoneally at day 21 with a homologous A. hydrophila strain and monitored for 14 days post-challenge. Vaccination was clinically well tolerated and improved survival relative to controls, with P4 showing the highest protection (RPS 81.8%). Agglutinating titres differed by treatment and time; at the peak sampling point (day 35), mean titres in P4 were ~200-fold higher than in P1, and model contrasts indicated significant differences versus controls (p<0.001). Splenic il-1β and ifn-γ transcript levels were higher in vaccinated groups than in controls at later time points. These findings support further evaluation of an oil-adjuvanted polyvalent inactivated A. hydrophila vaccine for gourami, including dose optimisation, extended safety assessment, heterologous challenge, and field validation.

Bacterial Abundance and Identification in Recirculating System of Sumatran Barb Fish Rearing Media with Duckweed as Biofilter Dita Wisudyawati; Ardha Nur Mustofa; Fajri Rahman Afif; Dinzidan Adiputra; Fata Habiburrahman; Md Afsar Ahmed Sumon; Abdul Manan; Rozi; Muhammad Hanif Azhar
Grouper Vol. 17 No. 1 (2026): Grouper: Jurnal Ilmiah Perikanan
Publisher : Universitas Islam Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30736/grouper.v17i1.409

The Sumatran Barb fish (Puntigrus tetrazona) is a freshwater ornamental fish which is highly demanded in the ornamental fish export market for its attractive colour patterns. The common problem in ornamental fish farming is the excessive use of water resources, increasing operational costs. A Recirculating Aquaculture System (RAS) is preferred since it can process water repeatedly and is more environmentally friendly. Duckweed plants (Lemna minor) can be used as a natural biofilter because they absorb nutrients such as nitrogen and phosphorus and provide a habitat for beneficial bacteria. This study was conducted for 40 days, with the treatments consisting of different plant coverage areas. Duckweed plants were applied with area coverage of P0/control (without duckweed plant), P1 (20% area coverage of duckweed plants), P2 (40% area coverage of duckweed plants), and P3 (60% area coverage of duckweed plants). The bacterial abundance values obtained during the maintenance period ranged from 1.52 to 1.79 x 106 CFU mL-1 and differed significantly (P<0.05) across all treatments. The lowest TPC value was observed in the P0 treatment (1.52 x 106 CFU mL-1), and the highest in the P2 treatment (1.79 x 106 CFU mL-1). Based on the identification test, bacteria belonging to the genera Neisseria, Acinetobacter, Nitrosomonas, and Pseudomonas were identified. Water quality parameters were optimal across all treatments, namely pH between 6.0 and 6.85, dissolved oxygen (3.43-3.95 mg L-1), TAN (0.2-0.4 mg L-1), and phosphate (1.00-2.5 mg L-1). Meanwhile, temperature and nitrate exceeded the optimal limits, with values ranging from 29 to 30 °C and 72 to 210 mg L-1 across all treatments.

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