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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) JFMR (Journal of Fisheries and Marine Research) Aquasains : Jurnal Ilmu Perikanan dan Sumberdaya Perairan Grouper: Jurnal Ilmiah Perikanan Jurnal Abdi Insani Jurnal Layanan Masyarakat (Journal of Public Service) Proceedings Series on Physical & Formal Sciences Balobe: Jurnal Pengabdian Masyarakat Journal of Fisheries & Marine Journal of Food and Agricultural Technology

Heru Pramono

Department Of Marine, Faculty Of Fisheries And Marine, Universitas Airlangga

Author-ID : 2562069

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Mechanical Engineering Social Sciences Veterinary Other

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IDENTIFIKASI DAN UJI AKTIVITAS ANTIBAKTERI KAPANG LAUT DIISOLASI DARI KORAL LUNAK FAVITES SP. Fanani, Ahmad Zainal; Novitasari, Tisa Ayu; Rofi’ah, Siti Choiriah; Dewi, Siti Nur Rukhoiyah; Pramono, Heru
BIOLINK : Jurnal Biologi Lingkungan, Industri dan Kesehatan Vol 6, No 1 (2019): Agustus
Publisher : Program Studi Biologi, Fakultas Biologi Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v6i1.2253

The aims of this study was to investigate the antimicrobial activity of fungi associated with coral. The fungi was cultured by streak plate method on potato dextrose agar (PDA) and reisolated with the same medium and were stored in slant agar for identified with morphological approach. Antibacterial activity fungi associated with coral was tested using overlay method against Escherichia coli and Staphylococcus aureus. Based on morphological analysis, five fungi were identified as Candidasp., Fusariumsp., Cladophialophorasp., Phaeoacremoniumsp., and Trichophytonsp. The antibacterial assay showed that Phaeoacremoniumsp. and Trichophytonsp. has antibacterial activity and potential as source of antibacterial for futher study.

IDENTIFIKASI DAN UJI AKTIVITAS ANTIBAKTERI KAPANG LAUT DIISOLASI DARI KORAL LUNAK FAVITES SP. Ahmad Zainal Fanani; Tisa Ayu Novitasari; Siti Choiriah Rofi’ah; Siti Nur Rukhoiyah Dewi; Heru Pramono
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 6, No 1 (2019): Agustus
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v6i1.2253

Isolation of Lactic Acid Bacteria That Produce Protease and Bacteriocin-Like Substance From Mud Crab (Scylla sp.) Digestive Tract (Isolasi Bakteri Asam Laktat yang Menghasilkan Protease dan Senyawa Bacteriocin-Like dari Saluran Pencernaan Kepiting Heru Pramono; Pipin Suciati; Sapto Andriyono
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 20, No 1 (2015): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Saluran pencernaan merupakan lingkungan kompleks yang terdiri atas berbagai spesies bakteri. Saluran pencernaan biota perairan terdiri atas bakteri aerob dan anaerob yang mampu memproduksi senyawa antibakteri dan enzim. Tujuan penelitian ini adalah untuk mengisolasi bakteri asam laktat yang menghasilkan protease dan senyawa bakteriosin-like dari saluran pencernaan kepiting bakau. Isolasi dan karakterisasi isolat dilakukan menggunakan media MRS. Supernatan netral bebas sel isolat telah diuji dengan menggunakan disc difusi agar terhadap bakteri patogen dan pembusuk. Uji produksi enzim protease telah diuji dengan metode disc diffusion agar menggunakan media kasein agar. Di antara seratus isolat, terdapat 96 isolat menunjukkan zona bening di MRS + CaCO3,, katalase negatif, dan bakteri Gram positif. Tiga puluh empat isolat bakteri asam laktat menghasilkkan protease dan hanya empat isolat (yaitu IKP29, IKP30, IKP52, dan IKP94) menunjukkan penghambatan yang kuat terhadap bakteri patogen dan pembusuk. Terdapat tiga pola inhibisi dari keempat isolate terhadap Bacillus subtilis, Staphylococcus aureus, Eschericia coli, dan Salmonella sp. Empat isolat tersebut berpotensi untuk dimanfaatkan sebagai starter pada produksi fermentasi produk hasil perikanan. Penelitian ini adalah penelitian pertama terkait isolasi bakteri asam laktat yang menghasilkan protease dan bakteriosin dari saluran pencernaan dari kepiting bakau.Kata kunci: Bakteri Asam Laktat, Bakteriosin-like substance, Protease, Scylla sp. Digestive tract is complex environment consist of large amount of bacteria’s species. Fish intestine bacteria consist of aerobic or facultative anaerob bacteria which can produce antibacterial and enzym. The objectives of this research were to isolated lactic acid bacteria that produce bacteriocin-like and protease from mud crab digestive tract. Isolation and characterization of isolates were conducted employing media MRS. Neutralized cell free supernatant of isolates were tested using disc diffusion agar of against pathogenic and spoilage bacteria to indicate bacteriocin-like-producing lactic acid bacteria. Protease-producing isolate was tested using disc diffusion method in casein agar. Among a hundred isolates, 96 isolates were showed clear zone in MRS+CaCO3,, catalase negative, and Gram positive bacteria. Thirty four isolates produced protease and only four isolates (i.e. IKP29, IKP30, IKP52, and IKP94) showed strong inhibition against pathogenic and spoilage bacteria. There were three patterns of inhibition among three isolates against Bacillus subtilis, Staphylococcus aureus, Eschericia coli, and Salmonella sp. All three isolates showed potential uses for produce starter culture for fishery product fermentation purpose. This is the first report of isolation lactic acid bacteria that produced protease and bacteriocin-like from digestive tract of mud crab. Keywords: Lactic acid bacteria, Bacteriocin-like substance, Protease, Scylla sp.

First Record of The Burrowing Goby Trypuchen vagina from Pangpang Bay, Indonesia Sapto Andriyono; Endang Dewi Masithah; Heru Pramono; Suciyono Suciyono
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 24, No 3 (2019): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

The burrowing goby, Trypauchen vagina, is recorded for the first time from the Pangpang Bay of Banyuwangi, Indonesia. Types of fish T. vagina is a type that has not published in Indonesia. This fish was caught using trap net in waters Pangpang Bay, which is an essential area in Banyuwangi, East Java. The description of the morphology and fish habitat conditions shows that the region has a diversity of fish resources that need attention. This goby fish has a local name as fish bedhul picek due to the sense of sight on these fish as if it does not work. Eyes covered by skin and socket head section was not found their eyeballs. The body of the specimens is elongate and reddish-pink in fresh specimens in coloration. There is no barbel on the chin. Cycloid scales are present on the whole body except for the head. Muncar people commonly consume T.vagina. This fish has been food sources in several regions but uncommon at several local markets in Indonesia. The T.vagina inhabit at Pangpang Bay as the bay, which has the high primary productivity of these waters due to sufficient nutrient support. Enrichment of nutrient comes from the run-off land and also the input of the number of fishing activity (embankment). An earlier report said that this fish found in waters Banyu Asin, Palembang, Sumatra, Indonesia. It also confirms that the fish found in this study are also living in a mud substrate that is reasonably smooth with right mangrove conditions around the bay, which close to Alas Purwo National Park.

INSEMINASI BUATAN MENGGUNAKAN PERSILANGAN SPERMA LIMOSIN DAN SIMENTAL MELALUI SINKRONISASI BIRAHI SUATU USAHA INTENSIFIKASI REPRODUKSI SAPI UNTUK PENINGKATAN PETERNAKAN SAPI RAKYAT DI KECAMATAN KEDUNGADEM KABUPATEN BOJONEGORO Emy Koestanti Sabdoningrum; Sunaryo Hadi Warsito; Heru Pramono; Siti Eliana
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 2 No. 1 (2018): Jurnal Layanan Masyarakat
Publisher : Universitas Airlangga

The beef cattle breeding business in Kedungrejo village, Kedungadem sub-district, Bojonegoro Regency in general has not implemented an efficient business concept considering that there are many potentials and opportunities that have not been utilized and managed optimally, especially those related to livestock reproduction and beef cattle production. Constraints that are often encountered are the low success rate of Artificial Insemination (IB) of beef cattle because poor quality cement and inseminator skills are not sufficient. Such conditions are also an illustration of the Beef Cattle Breeders in Kedungrejo Village, Kedungadem District, Bojonegoro Regency. Kedungrejo village was chosen to be a place for cattle development on dry land because it has the potential to breed cattle crosses of Lemosin and Simental cattle which produce good meat. The development of cattle in Kedungrejo village, Kedungadem sub-district, aims to increase economic activities in support of cattle reproduction intensification efforts to increase people’s cattle farming. This activity for the community aims to fulfill meat self-sufficiency through the introduction, dissemination, transfer of reproductive technology by artificial insemination using Limousine and Simental sperm crosses. Beef cattle farmers in Kedungrejo Village Kedungadem Bojonegoro Subdistrict have the prospect of beef cattle breeding which can be developed to fulfill meat self-sufficiency that is being promoted. Specific targets of outcomes to be achieved include the success of insemination, increased birth rate, improvement of genetic quality of beef cattle through Artificial Insemination using Limousine and Simental sperm crosses in the Kedungrejo Village, Kedungadem Bojonegoro District. The method used in achieving this goal by socializing Artificial Insemination using Limousine and Simental Sperm Crosses. The solution offered is through the coaching, training and application stages. The results of community service activities indicate that Artificial Insemination using Limousine and Simental Sperm Crosses has the prospect of being developed as an increase in the success of insemination, increased birth rate, genetic quality improvement has a positive influence on beef reproductive and meat self-sufficiency.AbstrakUsaha peternakan sapi potong di desa Kedungrejo Kecamatan Kedungadem Kabupaten Bojonegoro pada umumnya belum menerapkan konsep usaha yang efi sien mengingat banyak potensi dan peluang yang belum dimanfaatkan dan dikelola secara optimal terutama yang terkait reproduksi ternak dan produksi ternak sapi potong. Kendala yang sering dijumpai adalah rendahnya tingkat keberhasilan Inseminasi Buatan (IB) ternak sapi potong karena kualitas semen yang kurang baik dan skill inseminator belum memadai. Kondisi demikian juga merupakan gambaran dari Peternak Sapi Potong Desa Kedungrejo Kecamatan Kedungadem Kabupaten Bojonegoro. Desa Kedungrejo dipilih menjadi tempat pengembangan ternak sapi di lahan kering karena mempunyai potensi untuk pemuliabiakan persilangan sapi Lemosin dan sapi Simental yang menghasilkan daging yang bagus. Pengembangan ternak sapi di desa Kedungrejo Kecamatan Kedungadem bertujuan meningkatkan kegiatan perekonomian dalam mendukung usaha intensifi kasi reproduksi sapi untuk peningkatanpeternakan sapi rakyat. Kegiatan kepada masyarakat ini bertujuan pemenuhan swasembada daging melalui pengenalan, penyebarluasan, alih teknologi reproduksi dengan cara Inseminasi buatan menggunakan persilangan sperma Limousin dan Simental. Peternak sapi potong Desa Kedungrejo Kecamatan Kedungadem Bojonegoro mempunyai prospek peternakan sapi potong yang dapat dikembangkan untuk memenuhi swasembada dagingyang sedang digalakkan. Target khusus luaran yang ingin dicapai berupa keberhasilan inseminasi, peningkatan angka kelahiran, perbaikan mutu genetik sapi potong melalui Inseminasi Buatan menggunakan persilangan sperma Limousin dan Simental di wilayah Desa Kedungrejo Kecamatan Kedungadem Bojonegoro. Metode yang dipakai dalam pencapaian tujuan tersebut dengan sosialisasi Inseminasi Buatan menggunakan Persilangan Sperma Limousin dan Simental. Solusi yang ditawarkan adalah melalui tahap pembinaan, pelatihandan aplikasi. Hasil kegiatan pengabdian kepada masyarakat ini menunjukkan bahwa Inseminasi Buatan menggunakan Persilangan Sperma Limousin dan Simental mempunyai prospek untuk dikembangkan sebagai peingkatan keberhasilan inseminasi, peningkatan angka kelahiran, perbaikan mutu genetik memberi pengaruh positif terhadap reproduktivitas sapi potong dan pemenuhan swasembada daging.

PENDEKATAN PENINGKATAN PRODUKSI LELE PADA KELOMPOK PEMBUDIDAYA IKAN SURYA PERKASA DESA GENDONGKULON, BABAT, LAMONGAN MELALUI IMPLEMENTASI PROBIOTIK DAN TEKNOLOGI PASCAPANEN Heru Pramono; Dwi Yuli Pujiastuti; Sofi Maulana; Abdul Azis
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 2 No. 1 (2018): Jurnal Layanan Masyarakat
Publisher : Universitas Airlangga

Food production through the cultivation industry is one of the promising options because it is able to support the nutritional needs of the community, especially in terms of the adequacy of animal protein. One promising aquaculture product is catfish culture (Clarias batracus) because catfish are known to be able to grow in relatively unfavorable water conditions, have relatively short growth times (2–3 months). Nonetheless, the emergence of diseases that cause mass death and decreased cultivator income have become the main problems lately. Therefore, the purpose of community service is to overcome mass death in order to increase catfish production and diversify post-harvest fish processing to increase community income. The approach includes isolation and characterization of bacteria that cause mass death, bacterial resistance to antibiotics, indigenous probiotic production, and training in making catfish. The results of the dedication showed that there were bacteria that were resistant to antibiotics from isolation from catfish that experienced disease during July to October. In addition, efforts have been made to produce indigenous probiotics from the catfish digestive tract that survive mass death, and training in processing catfish snacks on site. A good response is shown in processing training and it is hoped that in the future these two efforts will be able to increase the economic independenceof the community.AbstrakProduksi pangan melalui industri budidaya merupakan salah satu opsi menjanjikan karena mampu mendukung kebutuhan gizi masyarakat, terutama dari segi kecukupan protein hewani. Salah satu produk budidaya yang menjanjikan adalah budidaya ikan lele (Clarias batracus) karena ikan lele dikenal mampu tumbuh dalam kondisi air yang relatif kurang baik, memiliki waktu pertumbuhan relatif pendek (2-3 bulan). Meskipun demikian munculnya penyakit yang menyebabkan kematian massal serta penghasilan pembudidaya yang menurunmenjadi permasalahan utama belakangan ini. Oleh karena itu, tujuan pengabdian kepada masyarakat ini adalah upaya mengatasi kematian massal guna meningkatkan produksi lele serta diversifi kasi olahan ikan pasca panen untuk meningkatkan penghasilan masyarakat. Pendekatan yang dilakukan meliputi isolasi dan karakteristik bakteri penyebab kematian massal, resistansi bakteri terhadap antibiotik, produksi probiotik indigenous, serta pelatihan pembuatan olahan lele. Hasil pengabdian menunjukkan bahwa terdapat bakteri yang tahan terhadap antibiotik hasil isolasi dari lele yang mengalami penyakit selama bulan Juli sampai Oktober. Selain itu juga telah dilakukan upaya produksi probiotik indigenous dari saluran pencernaan lele yang bertahan hidup dari kematian massal, dan pelatihan pengolahan snack berbahan ikan lele di lokasi. Respons yang baik ditunjukkan pada pelatihan pengolahan serta diharapkan ke depan kedua upaya ini mampu meningkatkan kemandirian ekonomi masyarakat.

PENINGKATAN ANGKA KONSUMSI IKAN MELALUI DIVERSIFIKASI PRODUK OLAHAN IKAN DI KECAMATAN BANYUATES KABUPATEN SAMPANG Kustiawan Tri Pursetyo; Pulung Siswantara; Annur Ahadi Abdillah; Heru Pramono; Eka Saputra
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 2 No. 2 (2018): Jurnal Layanan Masyarakat
Publisher : Universitas Airlangga

The Community Service Program aims to increase the knowledge of fish fishermen groups through counseling and training, then the Airlangga University Community Service Team transfers technology transfer to fishery product processing communities in Banyuates District, Sampang Regency. As target audiences that will be included in this Community Service program are the chairman, management and fish fishermen group, local village officials and local Animal Husbandry Service officers who are expected to later act as motivators. Itis hoped that later it can be relied upon as a source for the sustainability of the technology transfer program. The method used in the implementation of community service activities is by way of; (1) Development and training phase; (2) Monitoring and evaluation phase, To evaluate the level of knowledge and understanding of the material provided, it is given pretest and posttest. This Community Service program will be carried outfrom June to October 2017. To improve fish consumption, first the implementation team conducts socialization activities to elementary students to get to know more about various kinds of fish and their benefits. After thesocialization activities, counseling activities and training were made on various fish-based processed products, using the tutorial method, then continued with discussion.AbstrakProgram Pengabdian Kepada Masyarakat ini bertujuan untuk meningkatkan pengetahuan para kelompok nelayan ikan melalui penyuluhan dan pelatihan, maka Tim Pengabdian Kepada Masyarakat Universitas Airlangga melakukan transfer alih teknologi bagi masyarakat pengolahan produk perikanan di Kecamatan Banyuates, Kabupaten Sampang. Sebagai khalayak sasaran yang akan diikutsertakan dalam program Pengabdian Kepada Masyarakat ini adalah ketua, pengurus serta kelompok nelayan ikan, aparat desa setempat dan petugas Dinas Peternakan setempat yang diharapkan nantinya dapat bertindak sebagai motivator.Diharapkan nantinya dapat diandalkan sebagai sumber untuk kesinambungan program alih teknologi. Metode yang digunakan dalam pelaksanaan kegiatan pengabdian kepada masyarakat ini adalah dengan cara; (1) Tahap Pembinaan dan pelatihan; (2) Tahap monitoring dan evaluasi, Untuk mengevaluasi tingkat pengetahuan dan pemahaman terhadap materi yang diberikan maka diberikan pretest dan post test. Kegiatan program Pengabdian Kepada Masyarakat ini akan dilaksanakan mulai bulan Juni sampai dengan Oktober 2017. Untuk meningkatkan angka konsumsi ikan, terlebih dahulu Tim pelaksana kegiatan melakukan sosialisasi kepada siswa SD agar lebih mengenal tentang berbagai macam ikan beserta manfaatnya. Selepas kegiatan sosialisasi dilakukan kegiatan penyuluhan dan pelatihan pembuatan aneka produk olahan berbahan dasar ikan, dengan menggunakan metode tutorial, kemudian dilanjutkan dengan diskusi.

REDUCTION OF PATHOGENIC BACTERIA DURING FERMENTATION OF MASIN BY PROTEASE AND BACTERIOCIN-PRODUCING LACTIC ACID BACTERIA Heru Pramono; Pipin Suciati; Taruna Fernando Putra; Nova Andika; Sri Utari
AQUASAINS Vol 7, No 1 (2018)
Publisher : Jurusan Perikanan dan Kelautan

Contamination of pathogenic and spoilage bacteria on fermentation process of seafood and fisheries product is a major concern on food safety. The aims of this study were isolating and applying the bacteriocin- and protease-producing lactic acid bacteria from the gastrointestinal tract of mud crab for starter culture of masin, a traditional fermented shrimp from East Java. This study consisted of characterization of lactic acid bacteria, the application on the fermentation process and microbial analysis. Ninety-four isolates were isolated from mud crab was screened for the bacteriocin and protease producing as well as characterized by pH, salinity and biochemical. Isolate IKP-29 was exhibited strong protease and bacteriocin activity. Application of Isolate IKP-29 on masin fermentation showed that sharp reduction of Escherichia coli, Vibrio sp. and lactic acid bacteria counted. This study suggested that the application of lactic acid bacteria which producing bacteriocin and protease improved the food safety of traditional fermented fish.

EFFECT OF CHITOSAN COATINGS ON PRESERVATION OF RED SNAPPER (Lutjanus argentimaculatus Forsskal, 1775 ) DURING LOW TEMPERATURE STORAGE Heru Pramono; Tri Wahyuni; Faris Abidin; Wahyu Ardianto; Fareza Ferdyna Nanda Sumartono
JFMR (Journal of Fisheries and Marine Research) Vol 2, No 3 (2018): JFMR VOL 2 NO 3
Publisher : JFMR (Journal of Fisheries and Marine Research)

The effect of three different concentration of chitosan (0, 1.5 and 2 % w/v) on microbiological (aerobic plate count), chemicals (pH and total volatile basic nitrogen, TVB-N), and sensory properties of red snapper (Lutjanus argentimaculatus Forsskal, 1775) during cold storage (4-7oC) was evaluated periodically. The microbiological quality of red snapper coated with chitosan was lower compared to control, whereas pH and TVB-N were lower when treated with chitosan. The overall score of sensory evaluation of chitosan with concentration of 1% (w/v) was higher compared to remaining treatments. This study indicates that coating of chitosan prior to storage and distribution of red snapper prolong the shelf life of the fish but the microbial quality during storage is depend on initial quality of the fish.

Aktivitas Enzimatis Isolat Bakteri Asam Laktat dari Saluran Pencernaan Kepiting Bakau (Scylla spp.) Sebagai Kandidat Probiotik [Activity Enzymatic of Isolate Lactic Acid Bacteria from the Digestive Tract of Mud Crab (Scylla spp.) as a Candidate Probiotics] Wahju Tjahjaningsih; Endang Dewi Masithah; Heru Pramono; Pipin Suciati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 8 No. 2 (2016): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v8i2.11182

Abstrak Probiotik dapat didefinisikan sebagai mikroba hidup yang ditambahkan dalam jumlah tertentu yang mampu bertahan hidup dalam ekosistem saluran pencernaan. Enzim yang dihasilkan oleh mikroba yang diisolasi dari saluran pencernaan ikan dapat digunakan sebagai probiotik. Enzim proteolitik ekstraseluler secara alami diproduksi oleh mikroba untuk menghidrolisis polipeptida dalam media menjadi peptida dan asam amino. Bakteri asam laktat dapat menghasil enzim seperti protease, α-amilase, fitase, kitinase, lipase. Penelitian ini bertujuan untuk mendapatkan isolat bakteri asam laktat dari saluran pencernaan kepiting bakau (Scylla spp.) yang mempunyai aktivitas proteolitik. Penelitian ini menggunakan metode deskriptif berupa aktivitas enzimatis dan karakterisasi uji biokimia isolat bakteri asam laktat dari saluran pencernaan kepiting bakau (Scylla spp.). Jumlah kepiting bakau yang digunakan adalah 10 ekor. Hasil penelitian didapatkan tiga isolat bakteri asam laktat yang memiliki aktivitas proteolitik kuat, yaitu WK 28, WK 33, dan WK 53. Hasil uji biokimia isolat WK 28 termasuk ke dalam genus Pediococcus sp., isolat WK 33 termasuk ke dalam Lactobacillus sp., dan isolat WK 53 termasuk ke dalam genus Streptococcus sp. WK 28 (Pediococcus sp.) dan WK 33 (Lactobacillus sp.) mempunyai aktivitas proteolitik dan aktivitas lipolitik. Isolat WK 53 (Streptococcus sp.) mempunyai aktivitas enzimatis yaitu aktivitas proteolitik, amilolitik, dan lipolitik. Abstract Probiotics are defined as live microbes are added in a certain amount that is able to survive in the digestive tract ecosystem. Enzymes produced by microbes isolated from the digestive tract of fish can be used as probiotics. Extracellular proteolytic enzymes naturally produced by the microbes to hydrolyze a polypeptide in a media into peptides and amino acids. Lactic acid bacteria can produce enzymes such as proteases, α-amylase, phytase, chitinase, lipase. This study aims to get the lactic acid bacteria isolates from the gastrointestinal tract of mangrove crab (Scylla spp.) That have proteolytic activity. This research uses descriptive method such as enzymatic activity and biochemical characterization of isolates of lactic acid bacteria from the digestive tract of mangrove crab (Scylla spp.). Amount of mud crab used is 10 fish. The result showed three isolates of lactic acid bacteria that have a strong proteolytic activity, namely WK 28 WK 33 and WK 53. The results of biochemical tests WK 28 isolates belonging to the genus Pediococcus sp., Isolate WK 33 belonging to the Lactobacillus sp., And WK 53 isolates belonging to the genus Streptococcus sp. WK 28 (Pediococcus sp.) And WK 33 (Lactobacillus sp.) Have proteolytic activity and lipolytic activity. Isolates WK 53 (Streptococcus sp.) Have enzymatic activity is proteolytic activity, amylolytic, and lipolytic.

Co-Authors A. Shofy Mubarak Abdillah, Annur Ahadi Abdul Azis Abidin, Faris Adriana Monica Sahidu Agustono - Ahmad Zainal Fanani Alfi Hermawati Waskita Sari Alief Ayu Selia Alvianita Az-Zahra, Naela Amin, Muhamad Nur Ghoyatul Annisa, Amelia Nur Apriliyanto, Mochammad Bayu Arum, Kartika Dwi Sekar Bayu Kanetro Dewi, Siti Nur Rukhoiyah Dita Wisudyawati Dwi Yuli Pujiastuti Dwiyati Pujimulyani Eka Saputra Emy Koestanti Sabdoningrum Endang Dewi Masithah Fanani, Ahmad Zainal Fareza Ferdyna Nanda Sumartono Faris Abidin Fauziah, Aghnina Elvia Firmansyah, Muhammad Ichwan Hafizh M. Noor Haris, Mochamad Husein, Mohamad Akmal Alwi Islam, Nuruddin Haquel Larasati, Melani Mahrunisa, Aulia Dista Maulidia, Cici Miftakhul Ulum, Miftakhul N. Juni Triastuti Nova Andika Novitasari, Tisa Ayu Nuning Vita Hidayati Nur Aida Harahap Nuriyansyah Pangestu, Chandra Trisna Patmawati Patmawati, Patmawati Pipin Suciati Pipin Suciati Pipin Suciati Pulung Siswantara Purnama, Diva Frida Riesta Pursetyo, Kustiawan tri Putri, Monica Angelina Ramadhan, Novelix Arditan Rofi’ah, Siti Choiriah Rohmatin, Aulia Rokhim, Akhmad Nur Sapto Andriyono Siti Choiriah Rofi’ah Siti Eliana Siti Nur Rukhoiyah Dewi Siti Sahatul Fatimah Sofi Maulana Sri Utari Suciyono, Suciyono Sumartono, Fareza Ferdyna Nanda Tanti Dwi Oktavia Taruna Fernando Putra Tisa Ayu Novitasari Tjahjaningsih, Wahju tri wahyuni Tri Wahyuni Veryl Hasan Wahju Thajaningsih Wahyu Ardianto Wahyu Ardianto Warsito, Sunaryo Hadi Widiana, Hanidhan Dwiretno Wina Sapphira, Eunike Wulan, Novrida Yuli Pujiastuti, Dwi

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