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Nanoparticle-Enhanced 3D-Connector Microfluidic Paper-Based Analytical Device (3D-µPADs) for Sensitive and Cost-Effective Detection of Albumin-Creatinine Ratio in Urine Sample Sabarudin, Akhmad; Fiddaroini, Saidun; Fahmi, Ahmad Luthfi; Roja’i, Abdul Munir; Salsabila, Isadora Evani; Aulanni’am; Srihardyastutie, Arie; Susanti, Hani; Samsu, Nur
Science and Technology Indonesia Vol. 10 No. 2 (2025): April
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.2.504-518

Abstract

Chronic kidney disease (CKD) is a global health challenge affecting over 800 million people worldwide. Early detection is crucial to prevent progression to end-stage renal disease (ESRD), where life-saving interventions like dialysis or transplantation are necessary. Among the markers for early kidney damage, the Albumin Creatinine Ratio (ACR) in urine is one of the most reliable. Conventional methods of ACR detection, such as LC/MS-MS and ELISA, are highly accurate but require expensive equipment and skilled personnel, limiting their accessibility, especially in resource-limited settings. To address this, we developed a 3D-connector microfluidic paperbased analytical device (3D-µPADs) enhanced with gold nanoparticles (AuNPs) for sensitive and low-cost ACR detection. The integration of AuNPs amplifies colorimetric signals, enhancing the visual distinction in albumin detection. Our 3D-µPADs werefabricated using chromatographic paper Whatman No. 1 with hydrophobic barriers created by solid wax printing, followed by reagent immobilization for albumin and creatinine detection. The colorimetric and distance responses, based on reactions with Bromocresol Green (BCG) and Chrome Azurol S-Palladium (CAS-Pd2+), were analyzed using ImageJ software to quantify albumin and creatinine levels. The 3D-µPADs exhibited optimal sensitivity and accuracy, with linear detection ranges for albumin and creatinine of 30–400 mg/g. Validation with human urine samples demonstrated an accuracy of 93.04%, suggesting that 3D-µPADs offer a promising alternative for early nephropathy detection. Our findings provide a cost-effective, accessible tool for CKD screening, potentially transforming diagnostics in low-resource environments.
Sertifikasi JULEHA sebagai Upaya Peningkatan Ekonomi Masyarakat Kabupaten Lombok Barat Provinsi Nusa Tenggara Barat Herawati; Aulanni’am; Junining, Esti; Suprapto
Jurnal Gramaswara: Jurnal Pengabdian kepada Masyarakat Vol. 3 No. 3 (2023): Jurnal Gramaswara: Jurnal Pengabdian kepada Masyarakat
Publisher : Faculty of Cultural Studies, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.gramaswara.2023.003.03.06

Abstract

To support the implementation of the President’s instructions to boost investment in various regions in Indonesia, the West Lombok Regency Government, in the West Nusa Tenggara Province (NTB), through the Ministry of Agrarian and Spatial Planning/National Land Agency (ATR/BPN). Is striving to create an investmentfriendly climate through the Spatial Detail Plan (RDTR). Various elements that support the readiness of the RDTR program need to be prepared thoroughly, including the provision of standardized halal meat products, the application of science and technology, and mapping the potential at the Slaughterhouse (RPH) needs improvement. Additionally, important factors to consider include halal aspects and animal welfare to meet market needs where the resulting slaughtered livestockes meets the ASUH criteria (Safe, Healthy, Intact, and Halal). Unfortunately, only 10 butchers in the entire NTB have Veterinary Control Numbers (NKV) and are certified Halal Slaughterers (Juleha) by the National Certification Profession Agency (BNSP). The readiness of certified Juleha butchers needs to be improved to achieve the continuity of ASUH processed meat products. From this community service activity, there are a total of 20 butchers who have passed the test and obtained Juleha certification. The butcher program for butchers who have Juleha certification is a subsequent step that has been agreed upon by the community service team and the NTB Government.