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All Journal Media Kedokteran Hewan Jurnal Medik Veteriner
Kurniawan, Muhammad 'Ahdi
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Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Veterinary

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Morphometric and Molecular Identification of Eimeria Bovis and Eimeria Zuernii on Beef Cattle in Lamongan, East Java, Indonesia Kurniawan, Muhammad 'Ahdi; Suwanti, Lucia Tri; Mufasirin, Mufasirin; Suprihati, Endang; Hastutiek, Poedji; Kusnoto, Kusnoto; Ansori, Arif Nur Muhammad; Puspitasari, Yulianna; Khairullah, Aswin Rafif; Moses, Ikechukwu Benjamin; Pratama, Bima Putra; Riwu, Katty Hendriana Priscilia
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.153-166

Abstract

Lamongan Regency, located in East Java, Indonesia, is a significant center for beef cattle production. Despite its prominence, studies on the identification and differentiation of Eimeria spp. parasites in this region are notably lacking. This research aims to address this gap by evaluating the prevalence of Eimeria spp. and identifying two pathogenic species: Eimeria bovis and Eimeria zuernii. The study involved the collection of 250 fecal samples from beef cattle raised on smallholder farms across Lamongan. Sampling was conducted during the rainy season to optimize the detection of Eimeria infections. Using the sugar flotation method, Eimeria oocysts were isolated from 5–10 g of fecal matter per sample. Molecular identification employed polymerase chain reaction (PCR) assays targeting ribosomal RNA’s internal transcribed spacer 1 (ITS-1) region to detect the pathogenic species. Fecal examination using the Whitlock test revealed a prevalence of Eimeria spp. at 44.45% (111/250). PCR analysis further identified E. bovis with a 238 bp amplicon in Solokuro District and E. zuernii with a 344 bp amplicon in Tikung District, highlighting the presence of these two pathogenic species in distinct geographic areas. The findings underline the need for further research that includes expanded sampling from different regions and cattle breeds and the application of varied diagnostic methods. Such studies will contribute to a more comprehensive understanding of the genetic diversity, distribution, and epidemiology of E. bovis and E. zuernii in Indonesia, supporting improved cattle health and management practices in the region.
Analysis of Swallow Nest Nitrite Levels and Export Volume Projections: A Statistical Approach to Quality Improvement and Global Market Development Kusumawati, Anjung; Kurniawan, Muhammad 'Ahdi
Media Kedokteran Hewan Vol. 35 No. 3 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i3.2024.243-255

Abstract

The global SARS-CoV-2 pandemic is impacting public health and living systems. Edible Bird's Nest (EBN), one of the variants of Swallow's Nest (SBW), is recognized as a medicinal food. The potential of SBW as a therapeutic agent is gaining attention due to its ability to inhibit viral hemagglutination activity. This study provides information on the prediction of SBW demand in the coming year so that it can contribute to determining Indonesia's SBW export performance. Net weight data (tons) of SBW export time series were obtained for 10 years from 2012 to 2022 in 10 countries, namely: Hong Kong, China, Singapore, USA, Vietnam, Canada, Taiwan, Thailand, Japan, and Cambodia, combined with laboratory examination on nitrite levels conducted at the Surabaya Agricultural Quarantine Center, Indonesia. This study shows the minimum limit on nitrite levels in SBW exports and the need for SBW exports in the next four years. One of the requirements for SBW exports is the minimum limit of nitrite levels. The ARIMA method has been implemented to forecast SBW export demand in 10 countries for the next four years. SBW export demand in the next four years is expected to decline. The findings make a significant contribution as a source of information for decision-makers involved in SBW export activities.
Co-Authors Adi Sofyan Ansori, Muhammad Endang Suprihati Khairullah, Aswin Rafif Kusnoto Kusnoto, Kusnoto Kusumawati, Anjung Lucia Tri Suwanti, Lucia Tri Moses, Ikechukwu Benjamin Mufasirin Poedji Hastutiek Pratama, Bima Putra Riwu, Katty Hendriana Priscilia Yulianna Puspitasari