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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Sain Peternakan Indonesia Jurnal Medik Veteriner Cendana Medical Journal Jurnal Veteriner Nusantara Buletin Pengabdian
Riwu, Katty Hendriana Priscilia
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary

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Pattern of Antibiotic Resistance in Salmonella sp. Bacteria Contaminating Fresh Faeces of Laying Hens in Kediri District, West Lombok Regency Ramdani, Fitrah Akbar; Riwu, Katty Hendriana Priscilia; Kholik; Mbura, Yonanda Verawati Haslinda; Zakarias, Herdin Vanek
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2024): June 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.7814

Abstract

Antibiotic resistance has become a common problem in poultry farming in Indonesia. One of them is caused by improper use of antibiotics. This study aims to determine the pattern of resistance of Salmonella sp. isolated from laying hens to antibiotics. The samples used in this study were thirty samples of fresh faeces of laying hens from Kediri District, West Lombok Regency. The samples were isolated and identified through Gram staining and biochemical tests (indole, methyl red-Voges Proskauer, triple sugar iron agar, citrate, and urease), resulting in ten positive samples of Salmonella sp. The study continued with antibiotic resistance testing using disc diffusion or Kirby-Bauer methods. The antibiotics used in the study were aztreonam, ciprofloxacin, streptomycin, chloramphenicol, and tetracycline. The results showed that Salmonella sp. has been resistant to some antibiotics. The highest resistance levels are streptomycin and tetracycline at 50% (5/10), while the lowest is ciprofloxacin at 10% (1/10). Antibiotics that are still sensitive are aztreonam 90% (9/10) and chloramphenicol 100% (10/10). The findings of this study conclude that there is a pattern of antibiotic resistance in laying hens farms that can have a negative impact on human and animal health.
Antibiotic Resistance in Escherichia Coli Bacteria Isolated from Water Sources and Waste Disposal in Livestock Farms in East Lombok Sa’diyah, Siti Nurus; Kholik, Kholik; Munawaroh, Muhammad; Aprianti, Aurira Thrisma Dwi; Rahmawati, Septiyana Eka; Riwu, Katty Hendriana Priscilia
Jurnal Sain Peternakan Indonesia Vol 18 No 4 (2023)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.18.4.235-241

Abstract

Poor antimicrobial stewardship in livestock farms will lead to the emergence of bacterial resistance to antibiotics. Dug wells as a water source and waste disposal on livestock farms have a close distance, allowing transmission of Escherichia coli-resistant bacteria through soil absorption and fecal contamination. This study aims to isolate Escherichia coli from water sources and livestock waste disposal in East Lombok Regency and determine their sensitivity to several antibiotics. The type of this research is a descriptive cross-sectional survey using four water wells and four waste disposals with a criterion of < 10m range. Escherichia coli bacteria were isolated using culture techniques on Eosin Methylene Blue Agar, and identification was carried out using gram staining and biochemical tests. Determining sensitivity to antibiotics was performed using the Kirby-Bauer disk-diffusion method. The results showed that Escherichia coli bacteria have been isolated from water sources and waste disposal on livestock farms in East Lombok Regency. 100 % Escherichia coli isolates sensitive to Gentamicin, Ciprofloxacin, and Cefotaxime. 87.5% Escherichia coli isolates sensitive to Oxytetracycline, 12.5% ​​Escherichia coli isolates resistant to Oxytetracycline, 100% Escherichia coli isolates resistant to Penicillin G. Escherichia coli bacteria isolated from water sources and waste disposal at a livestock farm in East Lombok.
Peningkatan pengetahuan untuk pencegahan stunting melalui sosialisasi dan demo pembuatan makanan balita berbahan dasar protein hewani bagi kelompok istri peternak sapi pedaging rumahan di Desa Lando, Lombok Timur, Nusa Tenggara Barat [Increasing knowledge for stunting prevention through socialization and demonstration for creating animal protein-based toddler food for a group of wives of home-based beef cattle farmers in Lando Village, East Lombok, West Nusa Tenggara] Qurnianingsih, Ema Ema; Rusyda, Aqila sabila; Hamzah, Arshq Mirza; Rachmawati, Kadek; Humairah, Ira Ira; Lukitasari, Lina; Khaerunnisa, St; Wungu, Citrawati Dyah Kencono; Ummah, Fithriyah Cholifatul Ummah; Kholik, Kholik; Riwu, Katty Hendriana Priscilia; Pradana, Munawer; Yansri, Alifianita Anake; Hidayat, Muhammad; Prabowo, Gwenny Ichsan
Buletin Pengabdian Bulletin of Community Services Vol 4, No 3 (2024): Bull. Community. Serv.
Publisher : The Institute for Research and Community Services (LPPM) Universitas Syiah Kuala (USK)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/bulpen.v4i3.40293

Abstract

Stunting is a condition of failure to thrive in children under five (toddlers) which can hinder the child's physical and mental development. Data from the 2023 Indonesian Health Survey (SKI) shows that the number of stunted toddlers in West Nusa Tenggara (NTB) Province is still quite high, namely 24.6%. Stunting cannot be cured but can be prevented. One of the prevention efforts is to increase the knowledge of mothers or prospective mothers regarding stunting and preventing it by providing food with high nutrition. This Community Service Activity (PKM) was carried out with the aim of educating mothers and wives of home-based beef cattle farmers in Lando Village, East Lombok, NTB about preventing stunting by providing highly nutritious supplementary food (PMT) and stunting detection. The methods used were socialization and demonstration of making toddler food. The results of this PKM activity were that there was a significant increase in participants' knowledge regarding the material being socialized (p0.0001). The conclusion that can be drawn is that There had been an increase in knowledge among PKM participants regarding stunting and preventing stunting through providing food with high protein content.
Morphometric and Molecular Identification of Eimeria Bovis and Eimeria Zuernii on Beef Cattle in Lamongan, East Java, Indonesia Kurniawan, Muhammad 'Ahdi; Suwanti, Lucia Tri; Mufasirin, Mufasirin; Suprihati, Endang; Hastutiek, Poedji; Kusnoto, Kusnoto; Ansori, Arif Nur Muhammad; Puspitasari, Yulianna; Khairullah, Aswin Rafif; Moses, Ikechukwu Benjamin; Pratama, Bima Putra; Riwu, Katty Hendriana Priscilia
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.153-166

Abstract

Lamongan Regency, located in East Java, Indonesia, is a significant center for beef cattle production. Despite its prominence, studies on the identification and differentiation of Eimeria spp. parasites in this region are notably lacking. This research aims to address this gap by evaluating the prevalence of Eimeria spp. and identifying two pathogenic species: Eimeria bovis and Eimeria zuernii. The study involved the collection of 250 fecal samples from beef cattle raised on smallholder farms across Lamongan. Sampling was conducted during the rainy season to optimize the detection of Eimeria infections. Using the sugar flotation method, Eimeria oocysts were isolated from 5–10 g of fecal matter per sample. Molecular identification employed polymerase chain reaction (PCR) assays targeting ribosomal RNA’s internal transcribed spacer 1 (ITS-1) region to detect the pathogenic species. Fecal examination using the Whitlock test revealed a prevalence of Eimeria spp. at 44.45% (111/250). PCR analysis further identified E. bovis with a 238 bp amplicon in Solokuro District and E. zuernii with a 344 bp amplicon in Tikung District, highlighting the presence of these two pathogenic species in distinct geographic areas. The findings underline the need for further research that includes expanded sampling from different regions and cattle breeds and the application of varied diagnostic methods. Such studies will contribute to a more comprehensive understanding of the genetic diversity, distribution, and epidemiology of E. bovis and E. zuernii in Indonesia, supporting improved cattle health and management practices in the region.
Molecular Docking Analysis of Anti-dengue Activity of Moringa oleifera Leaves Bioactive Compounds Riwu, Audrey Gracelia; Jannah, Insani Fitrahulil; Riwu, Katty Hendriana Priscilia; Kale, Maria Laurenci Fanny Permata; Loe, Fhady Risckhy; Asa, Halena Meldy
Cendana Medical Journal Vol 12 No 2 (2024): Cendana Medical Journal
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/cmj.v12i2.23665

Abstract

Background: Dengue infection is an endemic disease in various tropical regions, including Indonesia, with increasing incidence and mortality rates, and currently, no specific therapy is available. One of the potential targets for therapeutic development is the dengue virus (DENV) envelope (E) protein, which plays a crucial role in viral replication in the host. Objective: This study aimed to explore the potential of bioactive compounds from Moringa oleifera leaves as inhibitors of the E protein through an in silico approach using molecular docking methods. Methods: A total of 17 bioactive compounds from Moringa oleifera leaf extract, based on previous studies, were obtained from the PubChem database, while the target protein structure was retrieved from the Protein Data Bank (RCSB). The drug-likeness properties of the compounds were evaluated using the SwissADME web tool. Molecular docking analysis was performed using PyRx Autodock, followed by 3D visualisation and ligand–protein interaction analysis using PyMOL and Discovery Studio 2.0. Apigenin, chrysin, kaempferol, and quercetin exhibited more negative binding affinity values than the control compound Celgosivir (-6.2 kcal/mol), with respective values of -7.4, -7.3, -7.4, and -7.3 Results: kcal/mol. These compounds also shared key amino acid residues with the control at the active site of the envelope protein. Moreover, all four compounds fulfilled the five drug-likeness criteria, indicating good oral bioavailability. Conclusion: Moringa oleifera leaves show potential as candidate anti-dengue agents through inhibition of the DENV envelope protein. Keywords: Moringa oleifera, molecular docking, dengue, antiviral, envelope protein
Studi In Silico: Potensi Senyawa Spesifik Kulit Batang Faloak (Sterculia quadrifida R. Br) sebagai Anti-Helmintik Riwu, Audrey Gracelia; Riwu, Katty Hendriana Priscilia; Pua Upa, Nurul Fatmawati; Kale, Maria Laurenci Fanny Permata; Jannah, Insani Fitrahulil; Wuhan, Yustinus Oswin Primajuni
Jurnal Veteriner Nusantara Vol 8 No 2 (2025): Agustus, 2025
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v8i2.25038

Abstract

Helminthiasis remains a significant global public health issue, particularly in tropical and subtropical regions. The extensive and repeated use of synthetic anthelmintic agents, including albendazole and mebendazole, has contributed to the emergence of drug resistance among various parasitic species. Therefore, the exploration of plant-derived compounds with potential anthelmintic activity is urgently needed. This study investigated the potential of bioactive compounds from the stem bark of Faloak (Sterculia quadrifida R. Br) as inhibitors of the Mitochondrial Rhodoquinol-Fumarate Reductase (MRFR) protein through an in silico approach. Five compounds: epicatechin, d-catechin, catechin-(4α→8)-catechin, spinasterol, and spinasterone were analyzed, with Atpeni as the control. Drug-likeness evaluation was conducted using Lipinski’s Rule of Five, while molecular docking, visualization and molecular interaction analysis was performed using PyRx, PyMOL, and Discovery Studio. Four compounds satisfied Lipinski’s criteria, indicating favorable pharmacokinetic properties. Among them, spinasterol and spinasterone demonstrated the strongest binding affinities to MRFR (–10.1 and –9.2 kcal/mol, respectively), exceeding that of the control ligand (–6.4 kcal/mol). Hydrophobic interactions involving key residues such as Leu146, Ala149, and Val153 contributed to complex stability. These results suggest that Faloak stem bark contains promising bioactive compounds that may serve as natural anti-helmintic candidates, warranting further in vitro and in vivo validation
Co-Authors . Kholik Adi Sofyan Ansori, Muhammad Aprianti, Aurira Thrisma Dwi Asa, Halena Meldy Audrey Gracelia Riwu Endang Suprihati Hamzah, Arshq Mirza Humairah, Ira Ira Jannah, Insani Fitrahulil Kadek Rachmawati Kale, Maria Laurenci Fanny Permata Khairullah, Aswin Rafif Kurniawan, Muhammad 'Ahdi Kusnoto Kusnoto, Kusnoto Loe, Fhady Risckhy Lucia Tri Suwanti, Lucia Tri Lukitasari, Lina Mbura, Yonanda Verawati Haslinda Moses, Ikechukwu Benjamin Mufasirin Muhammad Hidayat Munawaroh, Muhammad Poedji Hastutiek Prabowo, Gwenny Ichsan Pradana, Munawer Pratama, Bima Putra Pua Upa, Nurul Fatmawati Qurnianingsih, Ema Rahmawati, Septiyana Eka Ramdani, Fitrah Akbar Rusyda, Aqila sabila Sa’diyah, Siti Nurus Siti Khaerunnisa Ummah, Fithriyah Cholifatul Ummah Wuhan, Yustinus Oswin Primajuni Wungu, Citrawati Dyah Kencono Yulianna Puspitasari Zakarias, Herdin Vanek