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Moses, Ikechukwu Benjamin
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Biochemistry, Genetics & Molecular Biology Veterinary

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Detection of the CTX-M Gene Associated with Extended-Spectrum β-Lactamase (ESBL) in Broiler Chickens in Surabaya Traditional Markets Putri, Mariana Febrilianti Resilinda; Khairullah, Aswin Rafif; Effendi, Mustofa Helmi; Wibisono, Freshinta Jellia; Hasib, Abdullah; Moses, Ikechukwu Benjamin; Fauziah, Ima; Kusala, Muhammad Khaliim Jati; Raissa, Ricadonna; Yanestria, Sheila Marty
Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss2.2024.320-334

Abstract

A common indicator used to examine the frequency and distribution of antibiotic resistance against other enteric bacteria in humans and animals is the commensal enteric bacterium, Escherichia coli. The transmission of plasmids harboring ESBL enzymes, primarily generated by E. coli, is the cause of this resistance. The purpose of this study was to identify the CTX-M gene in ESBL-producing E. coli from broiler chicken cloacal swabs in traditional Surabaya markets. The samples used were 96 cloacal swabs from broiler chickens in the traditional markets of Dukuh Kupang, Keputran, Pacar Keling, and Pucang. The antibiotic disks used in this study belonged to five different antibiotic classes; they are aztreonam (monobactam), chloramphenicol (phenicol), kanamycin (aminoglycoside), ciprofloxacin (fluoroquinolone), and tetracycline (tetracycline). Presumptive ESBL strains were then molecularly screened for the presence of CTX-M gene. Results revealed that out of the 96 chicken cloacal swab samples collected, 58 (60.42%) were positive for E. coli based on morphological culture, Gram staining, and biochemical tests. Additionally, 15 out of the 58 E. coli isolates recovered from broiler chicken cloacal swabs were multidrug-resistant (MDR) while 7 of E. coli isolates harbored CTX-M gene. Conclusively, this study has shown that broiler chickens sold in traditional Surabaya markets harbor MDR E. coli which possess CTX-M gene. Conditions in traditional markets with low levels of cleanliness and chickens placed close together can spread resistance genes with serious public health consequences. Therefore, it is imperative to observe good hygienic practices in Surabaya traditional markets in order to curtail the spread of MDR bacterial pathogens in the food chain.
Prevalence of Ectoparasites in Tilapia (Oreochromis niloticus) in Sidoarjo, Indonesia Sari, Aulia Puspita; Koesdarto, Setiawan; Lestari, Tita Damayanti; Hastutiek, Poedji; Yunus, Muchammad; Herupradoto, Eduardus Bimo Aksono; Khairullah, Aswin Rafif; Ayuti, Siti Rani; Hasib, Abdullah; Aryaloka, Suhita; Moses, Ikechukwu Benjamin
Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss2.2024.370-381

Abstract

The problem most often encountered in fish farming is disease which can cause a decrease in fish production levels. Parasites cause one of the infectious diseases found in fish farming. The presence of parasites can cause a decrease in the quality of the growth of fish production. The purpose of this study was to identify the prevalence of ectoparasites on Nile tilapia in Sidoarjo, Indonesia. Ninety-six samples from two ponds were examined under the microscope to find out the presence of ectoparasite that exists in the outside body part of the fish. Samples were collected from skin, fins, tail, and gills using native methods and observed under a microscope in the Division of Parasitology Laboratory, Faculty of Veterinary Medicine, Universitas Airlangga. The results of this study showed 86 samples positively infected by ectoparasite and 10 showed negative. The ectoparasites found in this study were Trichodina sp., Chilodonella sp., Ichthyophthirius multifillis, Gyrodactylus sp., and Dactylogyrus sp. The highest prevalence is Trichodina sp. and Dactylogyrus sp. The number of fish infested by ectoparasite is 89.56%, this is classified in the usual category. The highest prevalence occurs in infestations of Trichodina sp. i.e.72.91% which were contained in the 70 samples. While the lowest prevalence was in Gyrodactylus sp. by 8.32% in 8 fish samples. Double infections from two different ponds were 70% within 33 samples which were included in the usual category.
Morphometric and Molecular Identification of Eimeria Bovis and Eimeria Zuernii on Beef Cattle in Lamongan, East Java, Indonesia Kurniawan, Muhammad 'Ahdi; Suwanti, Lucia Tri; Mufasirin, Mufasirin; Suprihati, Endang; Hastutiek, Poedji; Kusnoto, Kusnoto; Ansori, Arif Nur Muhammad; Puspitasari, Yulianna; Khairullah, Aswin Rafif; Moses, Ikechukwu Benjamin; Pratama, Bima Putra; Riwu, Katty Hendriana Priscilia
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.153-166

Abstract

Lamongan Regency, located in East Java, Indonesia, is a significant center for beef cattle production. Despite its prominence, studies on the identification and differentiation of Eimeria spp. parasites in this region are notably lacking. This research aims to address this gap by evaluating the prevalence of Eimeria spp. and identifying two pathogenic species: Eimeria bovis and Eimeria zuernii. The study involved the collection of 250 fecal samples from beef cattle raised on smallholder farms across Lamongan. Sampling was conducted during the rainy season to optimize the detection of Eimeria infections. Using the sugar flotation method, Eimeria oocysts were isolated from 5–10 g of fecal matter per sample. Molecular identification employed polymerase chain reaction (PCR) assays targeting ribosomal RNA’s internal transcribed spacer 1 (ITS-1) region to detect the pathogenic species. Fecal examination using the Whitlock test revealed a prevalence of Eimeria spp. at 44.45% (111/250). PCR analysis further identified E. bovis with a 238 bp amplicon in Solokuro District and E. zuernii with a 344 bp amplicon in Tikung District, highlighting the presence of these two pathogenic species in distinct geographic areas. The findings underline the need for further research that includes expanded sampling from different regions and cattle breeds and the application of varied diagnostic methods. Such studies will contribute to a more comprehensive understanding of the genetic diversity, distribution, and epidemiology of E. bovis and E. zuernii in Indonesia, supporting improved cattle health and management practices in the region.
Trypanosoma evansi as a Major Cause of Animal Trypanosomiasis: A Comprehensive Review Warsito, Sunaryo Hadi; Khairullah, Aswin Rafif; Lamid, Mirni; Al-Arif, Mohammad Anam; Hermadi, Herry Agoes; Lokapirnasari, Widya Paramita; Kusala, Muhammad Khaliim Jati; Wibowo, Syahputra; Ayuti, Siti Rani; Wardhani, Bantari Wisynu Kusuma; Fauziah, Ima; Yanestria, Sheila Marty; Moses, Ikechukwu Benjamin; Prasetyo, Agung; Aryaloka, Suhita; Fauzia, Kartika Afrida; Ahmad, Riza Zainuddin; Kurniasih, Dea Anita Ariani
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.455-477

Abstract

Trypanosomiasis caused by Trypanosoma evansi is a major protozoan illness that affects animals worldwide. It is also referred to as “surra” and affects a variety if wild and domestic animals such as sheep, cattle, goats, dogs, buffaloes, pigs, elephants, amongst others. In preparing this review, relevant scientific articles were searched on PubMed, SCOPUS, and Web of Science databases using the keyword “Trypanosoma evansi AND animals”. T. evansi are carried by a vast number of hematophagous flies and are found in the extracellular and internal fluids of certain hosts. Trypanosomosis is mostly characterized by anemia, and the degree of anemia can typically be used as a gauge for the disease's severity. Trypanosomiasis compromises the host animal's immune system and its diagnosis is dependent on a number of factors such as thorough clinical examination, suitable sample collection, sample size, suitable diagnostic test performance, and logical interpretation of test results. The clinical manifestations of trypanosomiasis vary widely in both appearance and severity, ranging from neurological disturbances and skin plaques to vaginal enlargement. Hematophagous biting flies, including Tabanus, Haematopota, Glossina, Chrysops, Lyperosia, Stomoxys, and Hippobusca flies, contribute to the spread of trypanosomiasis. Four medications are primarily used to treat trypanosomiasis: quinapyramine, karetin, diminazene aceturate (Berenil), and melarsomine (cymelarsan). An efficient vaccination program is an additional technique for managing infectious diseases in addition to treatment. The most important step in curtailing the spread of trypanosomiasis caused by T. evansi is to stop its transmission by flies via physical and chemical methods.
Co-Authors Adi Sofyan Ansori, Muhammad Agung Prasetyo Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Aryaloka, Suhita Ayuti, Siti Rani Endang Suprihati Fauzia, Kartika Afrida Fauziah, Ima Hasib, Abdullah Herry Agoes Hermadi Khairullah, Aswin Rafif Kurniasih, Dea Anita Ariani Kurniawan, Muhammad 'Ahdi Kusala, Muhammad Khaliim Jati Kusnoto Kusnoto, Kusnoto Lucia Tri Suwanti, Lucia Tri Mirni Lamid Mufasirin Mustofa Helmi Effendi Poedji Hastutiek Pratama, Bima Putra Putri, Mariana Febrilianti Resilinda Raissa, Ricadonna Riwu, Katty Hendriana Priscilia Riza Zainuddin Ahmad Sari, Aulia Puspita Setiawan Koesdarto Sheila Marty Yanestria Tita Damayanti Lestari Wardhani, Bantari Wisynu Kusuma Warsito, Sunaryo Hadi Wibisono, Freshinta Jellia Wibowo, Syahputra Widya Paramita Lokapirnasari Yulianna Puspitasari Yunus, Muchammad