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PENAMBAHAN BEBERAPA JENIS GULA DAPAT MENINGKATKAN KUALITAS SPERMATOZOA BEKU ASAL EPIDIDIMIS TERNAK DOMBA (Addition of Various Sugars in Improving Quality of Frozen Thawed Epididymal Spermatozoa of Ram) Herdis Herdis; I Wayan Angga Darmawan; Muhammad Rizal
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (353.204 KB) | DOI: 10.21157/j.ked.hewan.v10i2.5139

Abstract

ABSTRACT The purpose of this research was to examine the effect of various sugars addition in Tris extender on quality of frozen thawed epididymal spermatozoa of ram. Collected spermatozoa was divided into five tubes and centrifuged at 3,000 rpm for 30 minutes. Supernatants were removed and pellets were diluted with Tris extender (control), Tris extender + 0.4% dextrose (dextrose), Tris extender + 0.4% maltose (maltose), Tris extender + 0.4% lactose (lactose), and Tris extender + 0.4% sucrose (sucrose), respectively. There were no significantly difference among treatment on mean percentages of motile (MS), live (LS) and intact plasma membrane (IPM) after equilibration. Mean percentages of post-thawed MS, LS, and IPM for dextrose (43.0, 53.8, and 53.4%), maltose (44.0, 54.8, and 52.8%), lactose (44.0, 52.8, and 52.8%), and sucrose (44.0, 55.0, and 53.4%) were significantly (P0.05) higher than control (37.0, 44.0, and 40.8%). In conclusion, addition of 0.4% dextrose or 0.4% maltose or 0.4% lactose or 0.4% sucrose in Tris extender increased quality of frozen thawed cauda epididymal spermatozoa of ram.
EPIDIDYMAL SPERMATOZOA QUALITY OF ETAWA CROSSBREED GOAT IN TRIS EXTENDER SUPPLEMENTED WITH VARIOUS LACTOSE CONCENTRATIONS Muhammad Riyadhi; Agus Setiawan; Herdis Herdis; Muhammad Rizal
Jurnal Kedokteran Hewan Vol 11, No 1 (2017): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (225.168 KB) | DOI: 10.21157/j.ked.hewan.v11i1.5136

Abstract

This research was conducted to investigate the effect of various concentrations of lactose supplementation in Tris extender for maintaining the quality of Etawa crossbreed goat epididymal spermatozoa stored at 3-5° C. Semen in the control group was diluted with a tris extender containing 20% egg yolk without lactose. Semen in the test groups was diluted with a tris extender containing 20% egg yolk and added with 0.3% (0.3 g per 100 mL extender) and 0.6% lactose for group TL1 and TL2, respectively. Parameters evaluated of the fresh epididymal spermatozoa were motility, concentration, percentage of live, and abnormality of spermatozoa, while for diluted-spermatozoa were motility and percentage of live spermatozoa. Spermatozoa observation was conducted until it reaches 40% motility. The results showed that the mean percentage of motility, live sperm, concentration, and abnormality of epididymal spermatozoa were 70%; 81%; 3,220x106 cells/mL; and 4.30%, respectively in all group. After dilution, the percentage of motility and live spermatozoa were also 70% and 81.00±1.58%, respectively in all groups. The decreasing of spermatozoa motility was observed on day 4 of storage, in which percentage of spermatozoa motility in control group (40.00±0.00%) was significantly lower (P0.05) than those in TL1 (44.00±2.24%) and TL2(45.00±0.00%) groups. Percentage of live spermatozoa in control (63.20±2.68%) was not significantly different (P0.05) than TL1(65.40±1.95%) and TL2 (65.60±1.95%). In conclusion, the supplementation of lactose into Tris extender could maintain the epididymal spermatozoa of Etawa crossbreed for 3 days of storage at 3-5° C.
Cryopreservation of Aceh Cattle Semen with Date (Phoenix dactylifera) Extract Supplementation Yonadiah Dwitya; Kartini Eriani; Hendra Saputra; Al-Azhar Al-Azhar; Muhammad Rizal
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i1.18033

Abstract

Cryopreservation process could affect spermatozoa quality during from reactive oxygen species (ROS) produced in cellular metabolism and the environment. Spermatozoa damage caused by ROS during cryopreservation can be reduced with the addition of natural antioxidant which commonly found in fruits like date palm. This research was done to investigate the influence of date extract on semen quality after cryopreservation. This experimental study used a completely randomized design with 4 treatments and 6 replications. Semen collected from two aceh cattle bulls was diluted in tris egg yolk extender contained different concentrations (v/v) of date extract: 0% (P0, control), 0.75% (P1), 1% (P2), and 1.25% (P3) before cryopreserved at -196 ºC for 7 days. Semen quality prior to and after cryopreservation as well as sperm DNA integrity were determined by standard microscopic and laddering methods, respectively. The results showed that the addition of 1% date extract could maintain viability (68.67%), plasma membrane integrity (62.33%), and abnormality (18.58%) of aceh cattle spermatozoa, but unable to maintain its motility above 40%. There was no DNA fragmentation observed in both treated and fresh semen. This is the first study investigates the influence of supplementation of date palm extract on preserved aceh cattle spermatozoa diluted in egg yolk tris based extender.
DAYA HIDUP SPERMATOZOA KAMBING PERANAKAN ETAWAH YANG DIPRESERVASI DENGAN PENGENCER AIR TEBU Jodi Aulia Ramadhan; Muhammad Riyadhi; Nursyam Andi Syarifuddin; Anis Wahdi; Muhammad Rizal
Agrinimal Jurnal Ilmu Ternak dan Tanaman Vol 11 No 1 (2023): Agrinimal Jurnal Ilmu Ternak dan Tanaman
Publisher : Jurusan Peternakan Fakultas Pertanian Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ajitt.2023.11.1.45-50

Abstract

Air tebu dapat dimanfaatkan sebagai pengencer semen karena mengandung berbagai nutrien yang dapat menunjang kehidupan spermatozoa selama preservasi. Penelitian ini bertujuan untuk menguji kemampuan air tebu dalam mempertahankan daya hidup spermatozoa kambing PE selama preservasi pada suhu 3-5oC. Semen ditampung menggunakan vagina buatan dan segera dievaluasi secara makroskopis dan mikroskopis. Semen segar dibagi ke dalam lima buah tabung reaksi kemudain masing-masing diencerkan dengan 80% pengencer dasar laktosa + 20% kuning telur sebagai kontrol (P0), 20% air tebu + 60% akuabidetislata + 20% kuning telur (P1), 30% air tebu + 50% akuabidestilata + 20% kuning telur (P2), 40% air tebu + 40% akuabidestilata + 20% kuning telur (P3), dan 50% air tebu + 30% akuabidestilata + 20% kuning telur (P4). Semen yang telah diencerkan disimpan di dalam refrigerator lemari es pada suhu 3-5oC. Motilitas dan daya hidup spermatozoa masing-masing perlakuan dievaluasi setiap hari hingga motilitas mencapai 40%. Hasil penelitian diperoleh karakteristik semen segar kambing PE yaitu volume 1,15 ml, konsentrasi 3.870 juta/ml, motilitas 80%, spermatozoa hidup 87,75%, dan spermatozoa abnormal 7,5%. Persentase motilitas dan spermatozoa hidup pada hari keempat preservasi perlakuan P2 (41,25% dan 50%) nyata (P<0,05) lebih tinggi dibandingkan dengan P0 (37,5% dan 47%), P1 (32,5% dan 44%), P3 (30% dan 36,25%), dan P4 (18% dan 29,25%). Dapat disimpulkan bahwa Kombinasi pengencer terbaik untuk semen kambing PE adalah terdiri atas 30% air tebu + 50% akuabidestilata + 20% kuning telur. ABSTRACT Sugarcane juice can be used as an alternative semen extender because it contains various nutrients that can support the life of spermatozoa during preservation. This study aimed to examine the ability of sugarcane juice to maintain the ability of Etawah crossbreed goat spermatozoa during preservation at 3-5oC. Semen was collected using an artificial vagina and immediately evaluated macroscopically and microscopically. Fresh semen was divided into five tubes and diluted with 80% lactose-based extender + 20% egg yolk as a control (T0), 20% sugarcane juice + 60% aquabidestilata + 20% egg yolk (T1), 30% sugarcane juice + 50% aquabidestilata + 20% egg yolk (T2), 40% sugarcane juice + 40% aquabidestilata + 20% egg yolk (T3), and 50% sugarcane juice + 30% aquabidestilata + 20% egg yolk (T4), respectively. The diluted-semen was stored in the refrigerator at 3-5oC. The motility and viability of spermatozoa were evaluated every day until the motility reached 40%. The results showed that the characteristics of fresh semen of Etawah crossbreed goat were volume 1.15 ml volume, spermatozoa concentration 3870 million cells/ml, spermatozoa motility 80%, live spermatozoa 87.75%, and abnormal spermatozoa 7.5%. The percentage of motility and live spermatozoa on the fourth day of preservation for T2 (41.25% and 50%) was significantly (P<0.05) higher than T0 (37.5% and 47%), T1 (32.5% and 44%), T3 (30% and 36.25%), and T4 (18% and 29.25%). It can be concluded that the best extender combination for Etawah crossbreed goat semen is composed of 30% sugarcane juice + 50% aquabidestilata + 20% egg yolk.
Pemanfaatan Tepung Kulit Pisang Sebagai Bahan Pakan Alternatif Terhadap Kualitas Karkas Itik Raja N. Anita; Abrani Sulaiman; Muhammad Rizal
JURNAL PENELITIAN PETERNAKAN LAHAN BASAH Vol 2 No 1 (2015)
Publisher : www.ulm.ac.id

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jpplb.v2i1.1788

Abstract

Penelitian bertujuan untuk menguji pengaruh penggunaan tepung kulit pisang (TKP) sebagai bahanpakan alternatif pada ransum terhadap kualitas karkas yang meliputi: bobot potong, persentasekarkas, presentase giblet dan persentase lemak abdominal itik Raja. Metode yang digunakan adalahrancangan acak lengkap (RAL) dengan empat perlakuan dan lima kali ulangan. Jumlah itik Rajadalam penelitian ini adalah 60 ekor. Sebagai perlakuan dalam percobaan adalah KP0 (Tanpapenggunaan TKP dalam ransum), KP1 (penambahan 12% TKP dalam ransum), KP2 (penambahan24% TKP dalam ransum), dan KP3 (penambahan 36% TKP dalam ransum). Pakan yang diberikansesuai rekomendasi NRC. Pakan dan air minum diberikan ad libitum selama 42 hari percobaan.Data dianalisis menggunakan analisis ragam, dan jika menunjukkan pengaruh yang nyatadilanjutkan dengan uji beda nilai tengah menggunakan uji beda nyata terkecil. Hasil percobaanmenunjukkan bahwa penggunaan TKP pisang berpengaruh sangat nyata (P<0,01) terhadap bobotpotong, tidak berpengaruh nyata (P>0,05) terhadap persentase karkas, serta berpengaruh nyata(P<0,05) terhadap persentase giblet dan persentase lemak abdominal itik raja. Disimpulkan bahwapenggunaan TKP dapat menjadi bahan pakan alternatif dengan batasan penggunaan 10%-24%dalam ransum.