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All Journal JFMR (Journal of Fisheries and Marine Research) Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Juvenil: Jurnal Ilmiah Kelautan dan Perikanan
Alin Asyabil
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Engineering Environmental Science Materials Science & Nanotechnology Physics Social Sciences

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Komposisi Nuklotida Sekuen Gen rbcL pada Kappaphycus alvarezii yang dibudidaya di Jawa Timur Alin Asyabil; Yenny Risjani; Lawrence M. Liao
Jurnal Penelitian Pendidikan IPA Vol. 9 No. 11 (2023): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i11.4341

Abstract

Kappaphycus alvarezii seaweed is an important fishery commodity in Indonesia because it contains carrageenan, an important main ingredient in various industries. Research on the genetic characterization of seaweed in Indonesia is important as a source of information and genetic data needed to develop aquaculture practices such as breeding programs. Therefore, this study was conducted to determine the nucleotide composition of the rbcL gene sequence in Kappaphycus alvarezii as part of a genetic characterization study of Kappaphycus alvarezii seaweed in East Java. This study used five samples taken in Banyuwangi, Situbondo and Sumenep and compared them with the Kappaphycus alvarezii seaweed database at GenBank. The method used was the PCR method, and the primer pairs used were F-577 5'-GTATATGAAGGTCTAAAAGGTGG-3' and R-753 5'-GCTCTTTCATACATATCTTCC-3'. Based on research, primers F-577 and R-753 succeeded in amplifying the rbcL gene in Kappaphycus alvarezii samples in a partial manner with a length of 295 – 299 bp. The DNA sequence results of the sample have a similarity of 100% to the database in GeneBank. Nucleotide composition analysis shows the existence of genetic variations in the DNA sequences studied.
Komposisi Nuklotida Sekuen Gen rbcL pada Kappaphycus alvarezii yang dibudidaya di Jawa Timur Alin Asyabil; Yenny Risjani; Lawrence M. Liao
Jurnal Penelitian Pendidikan IPA Vol 9 No 11 (2023): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i11.4341

Abstract

Kappaphycus alvarezii seaweed is an important fishery commodity in Indonesia because it contains carrageenan, an important main ingredient in various industries. Research on the genetic characterization of seaweed in Indonesia is important as a source of information and genetic data needed to develop aquaculture practices such as breeding programs. Therefore, this study was conducted to determine the nucleotide composition of the rbcL gene sequence in Kappaphycus alvarezii as part of a genetic characterization study of Kappaphycus alvarezii seaweed in East Java. This study used five samples taken in Banyuwangi, Situbondo and Sumenep and compared them with the Kappaphycus alvarezii seaweed database at GenBank. The method used was the PCR method, and the primer pairs used were F-577 5'-GTATATGAAGGTCTAAAAGGTGG-3' and R-753 5'-GCTCTTTCATACATATCTTCC-3'. Based on research, primers F-577 and R-753 succeeded in amplifying the rbcL gene in Kappaphycus alvarezii samples in a partial manner with a length of 295 – 299 bp. The DNA sequence results of the sample have a similarity of 100% to the database in GeneBank. Nucleotide composition analysis shows the existence of genetic variations in the DNA sequences studied.
Peningkatan asupan protein hewani santri melalui edukasi gizi dan budidaya ikan di pondok pesantren Fasya, Arif Habib; Kenconojati, Hapsari; Budi, Darmawan Setia; Suciyono; Pardede, Maria Agustina; Kumalaningrum, Dwi Retna; Prayogo; Mauludia, Farida; Asyabil, Alin; Maulana, Muhammad Hilmy; Ambarwati, Dewi
Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Vol. 6 No. 3 (2025)
Publisher : Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jp2m.v6i3.23872

Abstract

Pengabdian kepada masyratakat ini dilaksanakan di Pondok Pesantren Al Anwari, Banyuwangi sebagai upaya untuk memenuhi kebutuhan protein hewani bagi para santri melalui budidaya ikan lele. Tujuan dari kegiatan ini adalah untuk memenuhi asupan protein hewani pada pondok pesantren serta  meningkatkan pengetahuan dan keterampilan santri dalam aspek teknis budidaya meliputi manajemen pakan hingga pasca panen. Metode kegiatan meliputi lima tahap yaitu penjaringan aspirasi, pelatihan, pendampingan, monitoring dan evaluasi serta dilakukan pengukuran kualitas air serta pretest maupun posttest. Pelaksanaan kegiatan melibatkan 49 santri kelas IX bersama pengelola pondok pesantren. Hasil pengukuran kualitas air menunjukkan nilai berada dalam kisaran normal untuk budidaya ikan lele. Nilai pretest berkisar antara 86,64-94,74 meningkat menjadi 88,60-96,49 pada posttest. Hasil ini menunjukkan bahwa pendekatan berbasis praktik dan pendampingan efektif dalam meningkatkan pengetahuan peserta. Kegiatan ini berpotensi dilakukan di lembaga pendidikan berbasis pesantren lainnya untuk mendukung ketahanan pangan dan meningkatkan ketrampilan santri.
Analysis of Hematological Profile of Catfish (Clarias sp.) as a Health Indicator Use of Hemolyzer for Aquatic Animals Pardede, Maria Agustina; Aziz, Muhammad Abdul; Apriliani, Dian Putri; Kumalaningrum, Dwi Retna; Ambarwati, Dewi; Maulidia, Farida; Asyabil, Alin; Maulana, Muhammad Hilmy
Juvenil Vol 6, No 4: November (2025)
Publisher : Department of Marine and Fisheries, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/juvenil.v6i4.27494

Abstract

ABSTRACTThe hematological profile of catfish (Clarias sp.) is a critical indicator of health and well-being in aquaculture practices. This study aimed to analyze the hematological parameters of catfish under aquaculture conditions using two different hematology analyzers: Hemolyzer 3 Pro and Veterinary Hematology Analyzer Mindray Series BC2800Vet. Blood samples were collected from the caudal vein, anticoagulated with EDTA, and analyzed for red blood cell count (RBC), white blood cell count (WBC), hemoglobin (Hb), hematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and platelet count (PLT). Results indicated significant variations between the analyzers. The Hemolyzer 3 Pro indicated elevated levels, with Hb ranging from 15.7 to 16.7 g/dl, RBC from 2.66 to 2.79 × 10^6/mm³, and WBC from 92,200 to 98,040 × 10^6/mm³, suggesting possible polycythemia and an active immune response. In contrast, the Mindray Analyzer reported values within the normal range, with Hb at 7.2 g/dl, RBC at 1.43 × 10^6/mm³, and WBC at 46,100 × 10^6/mm³, implying stable health conditions. The study also observed monocytosis, indicative of potential chronic inflammation or infection. These discrepancies highlight the importance of standardized methodologies in hematological assessments. Regular monitoring of hematological parameters is essential for early disease detection and effective management in aquaculture. Further research is recommended to explore the underlying causes of these variations and to optimize health management strategies for catfish in aquaculture systems.Keywords: Aquaculture, Catfish, Haemolyzer, Hematology
Pathology of Silver Rasbora (Rasbora argyrotaenia) After Injection of Polyhhenol From Kappaphycus alvarezii Kumalaningrum, Dwi Retna; Mauludia, Farida; Asyabil, Alin; Wahyudi, Diki; Amalya, Tria; Prayogi , M. Fahmi Dimas
JFMR (Journal of Fisheries and Marine Research) Vol. 9 No. 3 (2025): JFMR on November
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2025.009.03.9

Abstract

Rasbora argyrotaenia is an endemic Indonesian freshwater fish with high economic value. However, its population has decreased due to overexploitation and environmental pollution. One of the sustainable aquaculture approaches that is being developed is the utilisation of natural bioactive compounds such as polyphenol from Kappaphycus alvarezii seaweed. This study aimed to evaluate the pathological impact of polyphenol extract injections from K. alvarezii at various doses on the liver of silver rasbora.  The test fish were given injections into their abdomens at doses of 5.4, 7.2, and 9.0 mg/kg body weight. Tannic acid was used as the positive control, and no injection was given as the negative control. Histopathological results revealed hepatocyte degeneration and necrosis, which worsened at a dose of 9.0 mg/kg, indicating oxidative stress as the primary mechanism of tissue damage. Water quality parameters during the study were within the optimal range for fish life. These findings suggest that while polyphenols have the potential to act as immunostimulants, their use at high doses may cause significant liver toxicity. Therefore, their utilisation in aquaculture must be carefully managed and based on optimal dosages.
Co-Authors Amalya, Tria Ambarwati, Dewi Apriliani, Dian Putri DARMAWAN SETIA BUDI Dwi Retna Kumalaningrum Fasya, Arif Habib Kenconojati, Hapsari Lawrence M. Liao Maulana, Muhammad Hilmy Maulidia, Farida Mauludia, Farida Muhammad Abdul Aziz Pardede, Maria Agustina Prayogi , M. Fahmi Dimas Prayogo Suciyono WAHYUDI, DIKI Yenny Risjani