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Inhibition potency of drumstick leaf extract (Moringa oleifera) towards Aeromonas hydrophila: Preliminary Study for Aeromoniasis Treatment. Kenconojati, Hapsari; Rukman, Nina Rofi’
Journal of Aquaculture Science Vol 4, No 1 (2019): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.252 KB) | DOI: 10.31093/joas.v4i1.64

Abstract

The aim of this study was to know antibacterial potency of ethanolic extract of drumstick leaf against Aeromonas hydrophila in vitro. Total flavonoid, alkaloid, tannin and saponin of the ethanolic drumstick leaf extract were analyzed using spectrophotometry. Antibacterial activity test was carried out by disk diffusion and tube dilution method. Ethanolic extract of drumstick leaf contained flavonoids total as 71.9 mg quercetine equivalent/g, alkaloids total as 3 mg quinine equivalent/g, tannin as 24.7 mg tannic acid equivalent/g and saponin as 44.4 mg/g. The result of antibacterial test showed significant inhibition of Aeromonas hydrophila by drumstick leaf extract (P<0.05). The highest inhibition zone was produced by drumstick leaf extract with concentration of 100% which is 9.9± 0,162 mm. The minimum inhibition concentration (MIC) of drumstick leaf extract is 3.125%, while the minimum bactericidal concentration (MBC) is 6.25%. Based on this study, it can be concluded that drumstick leaf can be used as an alternative natural product of antibacterial agent which can be applied especially in aquaculture.
Bacterial Identification on Freshwater Fish Commodities at Fish Quarantine Center, Quality Control and Security of Fishery Products Surabaya I Rahayu, Nanik Ning; Prayogo, Prayogo; Ulkhaq, Mohammad Faizal; Kenconojati, Hapsari
Journal of Aquaculture Science Vol 4 No 2 (2019): Journal Of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v4i2.76

Abstract

The potential of freshwater fish cultivation in Indonesia is very large and is increasing every year. The high potential of freshwater and ornamental fisheries commodities and consumption must be developed through domestic and export marketing. The distribution carries the risk of entering and spreading fish pests and diseases, so is it required identification or quarantine is needed to avoid negative impacts from the distribution of fishery commodities. One of the infectious diseases which is known to attack fishery commodities in Indonesia is bacterial disease. Bacteria can cause high mortality in fish and loss for farmers. The purpose of this study is to identification bacteria in freshwater fish commodities. The study was conducted in December 2018 - January 2019 at the Fish Quarantine Center, Quality Control and Safety of Fisheries Products in Surabaya I. Identification of bacteria in this study using conventional methods. The parameters observed were external clinical symptoms that appeared on the body of the fish and internal pathology. The material examined in this study were The samples examined during Field Work Practices were Goldfish, Catfish fry, Common carp, and Koi fish with a total of 64 samples. This research was observative, then the data obtained were analyzed descriptively. The Result bacteria identified in freshwater fish commodities from 64 samples were dominated by Aeromonas hydrophila, Pseudomonas anguilliseptica, Streptococcus sp., Plesiomonas shigelloides, and Micrococcus sp.Keywords : Freshwater fisheries commodities, Bacteria, The Conventional Method
Virus Detection of Pacific White Shrimp (Litopenaeus vannamei) at Fish Quarantine Center, Quality Control, and Security of Fishery Product in Surabaya I Azizah, Aulia; Budi, Darmawan Setia; Fasya, Arif Habib; Kenconojati, Hapsari; Azhar, Muhammad Hanif
Journal of Aquaculture Science Vol 4, No 2 (2019): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (432.049 KB) | DOI: 10.31093/joas.v4i2.71

Abstract

Pacific white shrimp (Litopenaeus vannamei) is one of the leading fisheries commodities that have important economic value and are in great demand by consumers in the market. Pacific white shrimp has several advantages, namely rapid growth, able to adapt to a high range of salinity, and can be cultured with a super intensive system, but in recent years the total shrimp production in Indonesia has decreased. In 2012 the total shrimp production decreased from 1.900 tons to 1.025 tons, the virus is thought to be a pathogen that triggers disease in shrimp and causes high mortality. The type of virus that causes disease in pacific white shrimp cultivation is Taura Syndrome Virus (TSV), Infectious Myonecrosis Virus (IMNV), and White Spot Syndrome Virus (WSSV). The purpose of this study is to detect viruses that infected and determine the prevalence of viruses that attack pacific white shrimp. The research was conducted in December 2018 to January 2019 at Fish Quarantine Center, Quality Control and Security of Fishery Product Surabaya I. The material used in the study was 37 post larval samples. Samples extracted using Silica Extraction Kit, then detected molecularly using PCR (Polymerase Chain Reaction). This research was observational, then the data obtained were analyzed descriptively. The results showed that there were 3 positive samples of IMNV and 2 positive samples of WSSV with prevalence values of 8.10% and 5.40%. High and low prevalence values are influenced by the level of virus spread and inappropriate environmental conditions. Keywords : Pacific white shrimp, PCR (Polymerase Chain Reaction), prevalence, virus. 
The Insidensi dan Derajat Infeksi Anisakiasis pada Ikan Hasil Tangkapan di Pelabuhan Perikanan Pantai Muncar, Banyuwangi, Jawa Timur (INCIDENCE AND DEGREE OF ANISAKIASIS INFECTION IN FISH CATCHES AT THE FISHERY PORT OF MUNCAR BEACH, BANYUWANGI, EAST JAVA) Mohammad Faizal Ulkhaq; Darmawan Setia Budi; Muhammad Hanif Azhar; Hapsari Kenconojati
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.931 KB) | DOI: 10.19087/jveteriner.2019.20.1.101

Abstract

The purpose of this study was to determine the incidence and degree of infection anisakiasis in fish catches at Fishery Seaport Muncar, Banyuwangi as an effort to prevent food borne diseases in humans. Fish samples from Muncar Beach Fishery Seaport, Banyuwangi consisted of tuna fish (Euthynnus sp.), bloated fish (Rastrelliger sp.), giant trevally fish (Caranx sp.) and snapper (Lutjanus sp.) with total amount 192 tail. Sample fish was dissected and examined the parasite by native method. Parasitic worms was fixed with 5% glycerine alcohol, stained with SemichenAcetic Carmine method, identified by key identification and calculated incidence and degree of infection. The highest incidence and degree of infection showed in Lutjanus sp. and Rastrelliger sp. 66.67% and 5.39, respectively, while the lowest showed in Caranx sp. from 6.67% and 0.09 respectively. Continue research to determine incidence and degree of infection anisakiasis in each season was required to mapping and to know the spread of worms Anisakis sp.
Inhibition potency of drumstick leaf extract (Moringa oleifera) towards Aeromonas hydrophila: Preliminary Study for Aeromoniasis Treatment. Hapsari Kenconojati; Nina Rofi' Rukman
Journal of Aquaculture Science Vol 4 No 1 (2019): Journal Of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v4i1.64

Abstract

The aim of this study was to know antibacterial potency of ethanolic extract of drumstick leaf against Aeromonas hydrophila in vitro. Total flavonoid, alkaloid, tannin and saponin of the ethanolic drumstick leaf extract were analyzed using spectrophotometry. Antibacterial activity test was carried out by disk diffusion and tube dilution method. Ethanolic extract of drumstick leaf contained flavonoids total as 71.9 mg quercetine equivalent/g, alkaloids total as 3 mg quinine equivalent/g, tannin as 24.7 mg tannic acid equivalent/g and saponin as 44.4 mg/g. The result of antibacterial test showed significant inhibition of Aeromonas hydrophila by drumstick leaf extract (P<0.05). The highest inhibition zone was produced by drumstick leaf extract with concentration of 100% which is 9.9± 0,162 mm. The minimum inhibition concentration (MIC) of drumstick leaf extract is 3.125%, while the minimum bactericidal concentration (MBC) is 6.25%. Based on this study, it can be concluded that drumstick leaf can be used as an alternative natural product of antibacterial agent which can be applied especially in aquaculture.
Virus Detection of Pacific White Shrimp (Litopenaeus vannamei) at Fish Quarantine Center, Quality Control, and Security of Fishery Product in Surabaya I Aulia Azizah; Darmawan Setia Budi; Arif Habib Fasya; Hapsari Kenconojati; Muhammad Hanif Azhar
Journal of Aquaculture Science Vol 4 No 2 (2019): Journal Of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v4i2.71

Abstract

Pacific white shrimp (Litopenaeus vannamei) is one of the leading fisheries commodities that have important economic value and are in great demand by consumers in the market. Pacific white shrimp has several advantages, namely rapid growth, able to adapt to a high range of salinity, and can be cultured with a super intensive system, but in recent years the total shrimp production in Indonesia has decreased. In 2012 the total shrimp production decreased from 1.900 tons to 1.025 tons, the virus is thought to be a pathogen that triggers disease in shrimp and causes high mortality. The type of virus that causes disease in pacific white shrimp cultivation is Taura Syndrome Virus (TSV), Infectious Myonecrosis Virus (IMNV), and White Spot Syndrome Virus (WSSV). The purpose of this study is to detect viruses that infected and determine the prevalence of viruses that attack pacific white shrimp. The research was conducted in December 2018 to January 2019 at Fish Quarantine Center, Quality Control and Security of Fishery Product Surabaya I. The material used in the study was 37 post larval samples. Samples extracted using Silica Extraction Kit, then detected molecularly using PCR (Polymerase Chain Reaction). This research was observational, then the data obtained were analyzed descriptively. The results showed that there were 3 positive samples of IMNV and 2 positive samples of WSSV with prevalence values of 8.10% and 5.40%. High and low prevalence values are influenced by the level of virus spread and inappropriate environmental conditions. Keywords : Pacific white shrimp, PCR (Polymerase Chain Reaction), prevalence, virus. 
Bacterial Identification on Freshwater Fish Commodities at Fish Quarantine Center, Quality Control and Security of Fishery Products Surabaya I Nanik Ning Rahayu; Prayogo Prayogo; Mohammad Faizal Ulkhaq; Hapsari Kenconojati
Journal of Aquaculture Science Vol 4 No 2 (2019): Journal Of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v4i2.76

Abstract

The potential of freshwater fish cultivation in Indonesia is very large and is increasing every year. The high potential of freshwater and ornamental fisheries commodities and consumption must be developed through domestic and export marketing. The distribution carries the risk of entering and spreading fish pests and diseases, so is it required identification or quarantine is needed to avoid negative impacts from the distribution of fishery commodities. One of the infectious diseases which is known to attack fishery commodities in Indonesia is bacterial disease. Bacteria can cause high mortality in fish and loss for farmers. The purpose of this study is to identification bacteria in freshwater fish commodities. The study was conducted in December 2018 - January 2019 at the Fish Quarantine Center, Quality Control and Safety of Fisheries Products in Surabaya I. Identification of bacteria in this study using conventional methods. The parameters observed were external clinical symptoms that appeared on the body of the fish and internal pathology. The material examined in this study were The samples examined during Field Work Practices were Goldfish, Catfish fry, Common carp, and Koi fish with a total of 64 samples. This research was observative, then the data obtained were analyzed descriptively. The Result bacteria identified in freshwater fish commodities from 64 samples were dominated by Aeromonas hydrophila, Pseudomonas anguilliseptica, Streptococcus sp., Plesiomonas shigelloides, and Micrococcus sp.Keywords : Freshwater fisheries commodities, Bacteria, The Conventional Method
Microbiological Examination of Escherichia coli and Salmonella on Fishery Products at Fish Quarantine Station, Quality Control and Safety of Fishery Products Yogyakarta Mohammad Faizal Ulkhaq, S.Pi., M.Si; I.A. Nindi; H. Kenconojati; I.N. Putriantini; Inaiyah Inaiyah
Journal of Aquaculture Science Vol 6 No 2 (2021): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v6i2.106

Abstract

      Microorganisms that often contaminate fishery products were from bacteria groups. Some spesies of bacteria were harmful to human health because they can cause disease and even death in humans, such as Escherichia coli and Salmonella. Therefore its were needed for quarantine and microbiological examination of fishery products as an effort to prevent the spread of disease in humans. The aims of this study were to identify the contamination of E. coli and Salmonella bacteria in fishery products. A total eight samples of fishery products tested consist of canned dan frozen products using conventional methods based on ISO 16649-3: 2015 for E. coli and ISO 01-2332.2-2006 for Salmonella. The results showed that one from the six fishery products tested were frozen boiled tuna that positively E. coli and no fishery product sample was identified with Salmonella.
Identification of Aeromonas Salmonicida and Edwardsiella Ictalury in Live Fish that Will Be Trafficked from Yogyakarta Special Region Anggi Rusita Rahmawati; Mohammad Faizal Ulkhaq; Dewi Susanti; Hapsari Kenconojati; Arif Habib Fasya
Journal of Marine and Coastal Science Vol. 10 No. 2 (2021): JUNE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v10i2.27658

Abstract

The freshwater aquaculture fishery sector in Indonesia has the potential to be developed through extensification and intensification. The problem in aquaculture is diseases that infects cultivated organisms, one of which is caused by bacterial infection. Bacterial infection can cause huge losses to cultivation activities, including death which results in economic losses. The purpose of this study is to identify the A. salmonicida and E. ictalury in live fish that will be exported from DI Yogyakarta. The method of examining bacteria used is conventional which consists of isolation, purification, presumptive test, biochemical test, and identification. The results showed that all live fish samples (koi, gouramy, goldfish, catfish, and eel) were not infected with A. salmonicida and E. ictalury. This shows that all live fish are in a healthy condition and suitable to be exported of DI Yogyakarta.
Growth Response of Porphyridium sp. Culture on Glass and Plastic Container in Laboratory Scale Lutfiyah Al Adawiyah; Mohammad Faizal Ulkhaq; Hapsari Kenconojati
Journal of Aquaculture and Fish Health Vol. 9 No. 2 (2020): JAFH Vol. 9 No. 2 June 2020
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (305.941 KB) | DOI: 10.20473/jafh.v9i2.17183

Abstract

Utilization of Porphyridium sp. began to be developed as an anticancer, anti-inflammatory, antibacterial, antifungal, and production of biogas and biodiesel so that necessary to carry out culture activities for ensure continuity of Porphyridium sp. This study aims to determine the type of culture container that showed the best growth response of Porphyridium sp. with the highest biomass. The stages in this research were preparation of containers and media sterilization, making diatom fertilizers and agar media, Culture of Porphyridium sp. on agar media, Culture of Porphyridium sp. in the test tube, and Culture of Porphyridium sp. in glass and plastic containers. The results showed that the growth response of Porphyridium sp. that cultured in glass containers (18,9 ± 0,21 x 105 cells/mL) was higher than plastic (15.57 ± 0,03 x 105 cells/ml).
Co-Authors A. Shofy Mubarak A.A. Ketut Agung Cahyawan W Aditya, Yogi Putra Ahmad Alie Fikri Akhmad Taufiq Mukti Al-Musawa, Adining Tyas Aliyyah Revinda Bima Putri Ambarwati, Dewi Anggi Rusita Rahmawati Anggreani, Shovia Finny Arafik Lamadi Asyabil, Alin Audina Putri Geraldine Aulia Azizah Ayuda Dyah Nurekawati Citra Kurnia Putri DARMAWAN SETIA BUDI Daruti Dinda Nindarwi Dewi Ambarwati dewi susanti Dwi Retna Kumalaningrum Eka Saputra Eko Wahyudi Faizal Ulkhaq, Mohammad Fara Amalia Dewi Farahnovita Yuliyanti Farezi, Reza Adrio Fasya, Arif Habib Firdaus, Aji Akbar Fitriana, Evi Fransiska Okta Zania Gunanti Mahasri Habib Fasya, Arif Haditanojo, Wiyanto Hanif Azhara, Muhammad Herdiansyah, Akbar Dimas Hikam Zainul Qolbi I.A. Nindi I.N. Putriantini Ichi Arum Galerani Imlani, Ainulyakin Imlani, Ainulyakin Hasan Inaiyah Inaiyah Jayanti Dian Eka Sari, Jayanti Dian Kale Khairi Putik Nurikhwani KHOIRUN NISA Leni Mei Ristin Loh, Jiun-Yan Lutfiyah Al Adawiyah Mariah, Sharven Rao Maulana, Muhammad Hilmy Mauludia, Farida Mohammad Faizal Ulkhaq Mufasirin Muhammad Aidil Fitri Muhammad Thohawi Elziyad Purnama Muhammad Yusuf Taufiqur Rohman Müller, Tamás Muslimah, Bintang Nanik Ning Rahayu Nasution, Annio Indah Lestari Nina Rofi' Rukman Nurfaizah, Diza Ulya Pardede, Maria Agustina Praja, Ratih Novita Prameswari, Inco Ajeng Prayogo Prayogo Prayogo Prayogo, Prayogo Puspikawati, Septa Indra Rahayu, Nanik Ning Restanti, Maya Retno Septiwulan Rukman, Nina Rofi’ Rukmana, Nina Rofi Sabrina, Annisa Nur Sadida Anindya Bahtiar Saksono, Bayu Sapto Andriyono Shandy Sulistyoningrum Shierly Zaissiliya Clarissa Lifani Shovia Finny Anggraeni Shovia Finny Anggraeni Soetojo Soetojo Subhi Indriyani Suciyono Suciyono, Suciyono Suciyono, Suciyono Sukarno, Gerda Susilo, Rahadian Indarto Wahyu Nurlita Yudhana, Aditya