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All Journal JFMR (Journal of Fisheries and Marine Research) Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Segara Journal of Aquaculture and Fish Health Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Juvenil: Jurnal Ilmiah Kelautan dan Perikanan Dulang: Jurnal Pengabdian Kepada Masyarakat
Dwi Retna Kumalaningrum
Study Program Of Aquaculture, Departement Of Health And Life Science, School Of Health And Life Science, Universitas Airlangga, Banyuwangi, 68425, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Engineering Environmental Science Industrial & Manufacturing Engineering Materials Science & Nanotechnology Physics Social Sciences Other

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Exploration of Brachionus sp. Protein on the In Vivo Response of CD4 at Cantang Grouper (Epinephelus sp.) with VNN Infection (Viral Nervous Necrosis) Nur Fauziyah Martiningsih; Uun Yanuhar; Mohammad Musa; Dwi Retna Kumalaningrum
Journal of Aquaculture and Fish Health Vol. 12 No. 3 (2023): JAFH Vol. 12 No 3 September 2023
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v12i3.31497

Abstract

This study examines the function of Brachionus sp. protein on the Cluster Differentiation-4 (CD4) immune system in cantang grouper infected with VNN. The purpose of this study was to determine the protein content of Brachionus sp. which has the potential as an antivirus and to find out the benefits of Brachionus sp. on the expression of CD4 as an indicator of increased fish body defense system against VNN. Brachionus sp. itself contains three protein bands with molecular weights of 122.73 kDa, 75.49 kDa, and 13.77 kDa, which are expected to increase the immune system against VNN on cantang grouper. Protein injection of Brachionus with doses of 35 µl, 105 µl and 170 µl /150 g cantang grouper, showed that the lowest decrease was at a dose of 105 µl/150 gram cantang groupers.
Economic improvement and fish farming based on Smart Aquaculture Automatized System in Segobang Village, Banyuwangi Fasya, Arif Habib; Kenconojati, Hapsari; Budi, Darmawan Setia; Suciyono, Suciyono; Pardede, Maria Agustina; Kumalaningrum, Dwi Retna; Prayogo, Prayogo; Saputra, Eka; Firdaus, Aji Akbar; Maulana, Muhammad Hilmy; Ambarwati, Dewi
Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Vol. 10 No. 1 (2025): February 2025
Publisher : University of Merdeka Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26905/abdimas.v10i1.14811

Abstract

The fisheries and marine sector in East Java has abundant potential, one of which is Banyuwangi district. Banyuwangi Regency has potential in freshwater aquaculture because it has 324 rivers with an area of 735 km2 and swamps and reservoirs of 60 hectares. One of the villages in Banyuwangi Regency is Segobang Village, which has the potential to have abundant water sources and continuous flow. This potential can be utilized by training and assisting fish nurseries in Segobang Village by utilizing continuously flowing water and Smart Aquaculture Automatized System (SAAS) technology by utilizing biofilters and running water and aeration as a source of oxygen.  The stages carried out in this community service are as follows: initial assessment of the location, infrastructure and technology development, periodic training and mentoring, implementation and monitoring of the cultivation process, and continued marketing and sales. After the training, the Segobang Village community showed a significant increase in knowledge. The average pre-test scores ranged from 30 to 51, while the post-test scores increased to 79 to 87. This improvement shows that participants better understand the SAAS and its use in fish farming. Continued training and practice can help strengthen the adoption of this technology in the community.
Peningkatan asupan protein hewani santri melalui edukasi gizi dan budidaya ikan di pondok pesantren Fasya, Arif Habib; Kenconojati, Hapsari; Budi, Darmawan Setia; Suciyono; Pardede, Maria Agustina; Kumalaningrum, Dwi Retna; Prayogo; Mauludia, Farida; Asyabil, Alin; Maulana, Muhammad Hilmy; Ambarwati, Dewi
Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Vol. 6 No. 3 (2025)
Publisher : Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jp2m.v6i3.23872

Abstract

Pengabdian kepada masyratakat ini dilaksanakan di Pondok Pesantren Al Anwari, Banyuwangi sebagai upaya untuk memenuhi kebutuhan protein hewani bagi para santri melalui budidaya ikan lele. Tujuan dari kegiatan ini adalah untuk memenuhi asupan protein hewani pada pondok pesantren serta  meningkatkan pengetahuan dan keterampilan santri dalam aspek teknis budidaya meliputi manajemen pakan hingga pasca panen. Metode kegiatan meliputi lima tahap yaitu penjaringan aspirasi, pelatihan, pendampingan, monitoring dan evaluasi serta dilakukan pengukuran kualitas air serta pretest maupun posttest. Pelaksanaan kegiatan melibatkan 49 santri kelas IX bersama pengelola pondok pesantren. Hasil pengukuran kualitas air menunjukkan nilai berada dalam kisaran normal untuk budidaya ikan lele. Nilai pretest berkisar antara 86,64-94,74 meningkat menjadi 88,60-96,49 pada posttest. Hasil ini menunjukkan bahwa pendekatan berbasis praktik dan pendampingan efektif dalam meningkatkan pengetahuan peserta. Kegiatan ini berpotensi dilakukan di lembaga pendidikan berbasis pesantren lainnya untuk mendukung ketahanan pangan dan meningkatkan ketrampilan santri.
PROGRAM EDUKASI MANAJEMEN PENGOLAHAN IKAN BEREGULASI HALAL Qori'ah, Maria; Fatimah, Nurul; Andriyanto, Dicky; Kumalaningrum, Dwi Retna; Subhan, Danil Hamdani
Jurnal Pengabdian Masyarakat Polmanbabel Vol. 5 No. 02 (2025): DULANG : Jurnal Pengabdian Kepada Masyarakat
Publisher : Pusat Penelitian dan Pengabdian Kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33504/dulang.v5i02.720

Abstract

Fish products are a nutritious food alternative for families and have high market value. The nutrients in fish are important for the body, especially for children. However, these beneficial nutrients are inseparable from quality assessment and fish processing management. This community service activity aims to educate the public and MSME actors in recognising and processing fish properly. The material presented not only focused on fish processing, but also on increasing the selling value, which was the focus of the community service activity. The selling value of fish products increases when they receive a certificate as a product label reinforcement. Therefore, material on the halal product certification process is important to complement the fish processing management material so that consumers are confident that the product quality is guaranteed. The enthusiastic participants consisted of PKK cadres and SMEs under the guidance of the village government. The results of the activity showed an increase in participants' understanding, as measured by discussions and hands-on practice accompanied by resource persons. The findings of this program are that fish nuggets are a superior product that is healthy and nutritious and has halal regulations with a mixture of bread as a substitute for flour.
Immunological Response of Interleukin-6 on Cantang Hybrid Grouper (Ephinephelus sp.) By Induction Of Protein Brachionus sp. Kumalaningrum, Dwi Retna; Yanuhar, Uun; Musa, Mohammad; Martiningsih, Nur Fauzi-yah
Journal of Aquaculture and Fish Health Vol. 13 No. 3 (2024): JAFH Vol. 13 No. 3 September 2024
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v13i3.30835

Abstract

This study examines the role of Brachionus sp protein on the interleukin-6 pro-inflammatory immune system in Cantang grouper infected with Viral Nervous Necrosis (VNN). The purpose of this study was to determine the protein content of Brachionus sp. which has the potential as an antivirus and to find out the benefits of Brachionus sp. on the expression of Interleukin-6 as an indicator of increased fish body defense system against VNN. Testing of Brachionus protein by injection at doses of 35 µl, 105 µl, and 170 µl /150 gram Cantang grouper. The results showed that the lowest decrease was at a dose of 105 µl/150-gram Cantang groupers.  In addition, Brachionus sp. contains 3 protein bands with molecular weights of 122.73 kDa, 75.49 kDa, and 13.77 kDa. 
The Effect of Protein Induction of Brachionus sp. Toward In-Vivo Response of Cluster Differentiation-8 (CD8) Cantang Grouper (Epinephelus sp.) Infected by VNN (Viral Nervous Necrosis) Martiningsih, Nur Fauziyah; Yanuhar, Uun; Musa, Mohammad; Kumalaningrum, Dwi Retna
Jurnal Penelitian Pendidikan IPA Vol 9 No 8 (2023): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i8.4025

Abstract

The large demand for grouper conduce increase production intensively in various ways, by increasing the production of grouper aquaculture raises causing new problems, one of which is infection with VNN disease. The use of antibiotics to treat VNN infections is prohibited or no longer recommended by the government. An alternative way to deal with VNN infection is by increasing the body's immunity in the fish itself. One effort that can be done to increase the body's immunity in grouper is by administering Brachionus sp. protein extracts. This research was conducted to determine whether the Brachionus sp. has an effect on the immune system in cantang grouper (Epinephelus sp.) as indicated by the emergence of Cluster Differentiation-8 (CD8) expression in fish that have been infected with VNN. The proteins contained in Brachionus sp. that has three type molecular weight (122.73 kDa, 75.49 kDa, and 13.77 kDa) potential as an ingredient for treating VNN-infected fish. It is hoped that this can improve the immune system in Epinephelus sp. infected with VNN. Protein Brachionus sp. given by injection at a dose of 35 µl, 105 µl and 170 µl per 150 gr Epinephelus sp. 105 µl was the best concentration in boosting the immune system in the tested Epinephelus sp., as indicated by a decreased CD8 count.
IDENTIFICATION, ISOLATION, PURIFICATION, GRAM STAINING, AND BIOCHEMICHAL TESTING PROCESSES OF AQUATIC BACTERIA FROM DIFERENT SOURCES Pardede, Maria Agustina; Fasya, Arif Habib; Kumalaningrum, Dwi Retna; Ulkhaq, Mohammad Faizal; Kenconojati, Hapsari; Ambarwati, Dewi; Maulana, Muhammad Hilmy
Jurnal Segara Vol 19, No 3 (2024): December
Publisher : Politeknik Kelautan dan Perikanan Dumai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/segara.v19i3.15409

Abstract

Abstract
Analysis of Hematological Profile of Catfish (Clarias sp.) as a Health Indicator Use of Hemolyzer for Aquatic Animals Pardede, Maria Agustina; Aziz, Muhammad Abdul; Apriliani, Dian Putri; Kumalaningrum, Dwi Retna; Ambarwati, Dewi; Maulidia, Farida; Asyabil, Alin; Maulana, Muhammad Hilmy
Juvenil Vol 6, No 4: November (2025)
Publisher : Department of Marine and Fisheries, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/juvenil.v6i4.27494

Abstract

ABSTRACTThe hematological profile of catfish (Clarias sp.) is a critical indicator of health and well-being in aquaculture practices. This study aimed to analyze the hematological parameters of catfish under aquaculture conditions using two different hematology analyzers: Hemolyzer 3 Pro and Veterinary Hematology Analyzer Mindray Series BC2800Vet. Blood samples were collected from the caudal vein, anticoagulated with EDTA, and analyzed for red blood cell count (RBC), white blood cell count (WBC), hemoglobin (Hb), hematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and platelet count (PLT). Results indicated significant variations between the analyzers. The Hemolyzer 3 Pro indicated elevated levels, with Hb ranging from 15.7 to 16.7 g/dl, RBC from 2.66 to 2.79 × 10^6/mm³, and WBC from 92,200 to 98,040 × 10^6/mm³, suggesting possible polycythemia and an active immune response. In contrast, the Mindray Analyzer reported values within the normal range, with Hb at 7.2 g/dl, RBC at 1.43 × 10^6/mm³, and WBC at 46,100 × 10^6/mm³, implying stable health conditions. The study also observed monocytosis, indicative of potential chronic inflammation or infection. These discrepancies highlight the importance of standardized methodologies in hematological assessments. Regular monitoring of hematological parameters is essential for early disease detection and effective management in aquaculture. Further research is recommended to explore the underlying causes of these variations and to optimize health management strategies for catfish in aquaculture systems.Keywords: Aquaculture, Catfish, Haemolyzer, Hematology
Pathology of Silver Rasbora (Rasbora argyrotaenia) After Injection of Polyhhenol From Kappaphycus alvarezii Kumalaningrum, Dwi Retna; Mauludia, Farida; Asyabil, Alin; Wahyudi, Diki; Amalya, Tria; Prayogi , M. Fahmi Dimas
JFMR (Journal of Fisheries and Marine Research) Vol. 9 No. 3 (2025): JFMR on November
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2025.009.03.9

Abstract

Rasbora argyrotaenia is an endemic Indonesian freshwater fish with high economic value. However, its population has decreased due to overexploitation and environmental pollution. One of the sustainable aquaculture approaches that is being developed is the utilisation of natural bioactive compounds such as polyphenol from Kappaphycus alvarezii seaweed. This study aimed to evaluate the pathological impact of polyphenol extract injections from K. alvarezii at various doses on the liver of silver rasbora.  The test fish were given injections into their abdomens at doses of 5.4, 7.2, and 9.0 mg/kg body weight. Tannic acid was used as the positive control, and no injection was given as the negative control. Histopathological results revealed hepatocyte degeneration and necrosis, which worsened at a dose of 9.0 mg/kg, indicating oxidative stress as the primary mechanism of tissue damage. Water quality parameters during the study were within the optimal range for fish life. These findings suggest that while polyphenols have the potential to act as immunostimulants, their use at high doses may cause significant liver toxicity. Therefore, their utilisation in aquaculture must be carefully managed and based on optimal dosages.
Co-Authors Amalya, Tria Ambarwati, Dewi Apriliani, Dian Putri Asyabil, Alin DARMAWAN SETIA BUDI Dicky Andriyanto Eka Saputra Fasya, Arif Habib Firdaus, Aji Akbar Kenconojati, Hapsari Martiningsih, Nur Fauzi-yah Martiningsih, Nur Fauziyah Maulana, Muhammad Hilmy Maulidia, Farida Mauludia, Farida Mohammad Faizal Ulkhaq Mohammad Musa Muhammad Abdul Aziz Musa, Mohammad Nur Fauziyah Martiningsih Nurul Fatimah Pardede, Maria Agustina Prayogi , M. Fahmi Dimas Prayogo Prayogo, Prayogo Qori'ah, Maria Subhan, Danil Hamdani Suciyono Suciyono, Suciyono WAHYUDI, DIKI Yanuhar, Uun