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EFFICACY OF THE BIOFLOC SYSTEM WITH THE ADDITION OF CHITOSAN ON TIGER PRAWN (Penaeus monodon) PRODUCTIVITY Maulianawati, Diana; Amien, Muhammad; Pardi, Yusriadi; Hafid, Hafid; Rifai, Muhammad Aditya`; Raoda, Andini Aisyah Nur; Luthfiyana, Novi; Syahril, Syahril
BIOTROPIA Vol. 32 No. 2 (2025): BIOTROPIA Vol. 32 No. 2 August 2025
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2025.32.2.2482

Abstract

HIGHLIGHTS- Biofloc technology with chitosan addition improved the growth performance of Penaeus monodon, increased floc volume and heterotrophic bacteria, and reduced Vibrio-like bacteria.- Biofloc technology with chitosan addition enhanced water quality and microbial community, supporting P. monodon growth.P. monodon in a biofloc system with chitosan had higher weight gain, growth rate, and survival rate. - The study provides insights for sustainable intensive shrimp farming using biofloc technology.- Findings contribute to improving productivity and maintaining environmental conditions in shrimp aquaculture. ABSTRACTPenaeus monodon farming failures are often found from Post Larva's initial distribution phase until harvest. High mortality rates of P. monodon post-larvae are often observed after stocking. A disease attack in the second month during the rearing period eventually causes failure in extensive ponds. The primary objective of this research was to investigate the impacts of adding chitosan on on the yield of P. monodon within a biofloc technology (BFT) culture system. The experiment was carried out in triplicate using three distinct treatment options. Post-larval P. monodon (PL-10) that were visually healthy and disease-free were obtained from a hatchery and then stocked at a density of 40 individuals per 100 liters. Shrimps, and fed a commercial diet four times daily for 40 days. A statistically significant difference was observed (p-value < 0.05) in weight gain (0.213-0.299 g), average daily growth (0.0054-0.0074 g/day), and specific growth rate (13.4-14.3 %/day) of shrimp were found among the treatments. The inclusion of chitosan in the biofloc system led to a prominent rise in floc volume and heterotrophic bacterial populations, while simultaneously reducing the number of Vibrio-like bacteria (VLB) colonies. No notable variations were noted in food conversion ratio (FCR), total length, survival rate, total nitrite-N, nitrate-N, and total ammonia nitrogen levels. Biofloc technology supplemented with chitosan is found to boost the growth rate of P. monodon by enhanced both water quality and the microorganisms present within it, according to the study.
Comparative Analysis of the Efficacy of Bacillus subtilis and Lactoba-cillus acidophilus in Inhibiting Vibrio parahaemolyticus Infection in Black Tiger Shrimp (Penaeus monodon) Gusman, Ery; Raoda, Andini Aisyah Nur; Maulianawati, Diana; Rukisah, Rukisah; Zainuddin, Zainuddin; Imra, Imra
Journal of Aquaculture and Fish Health Vol. 15 No. 1 (2026): JAFH Vol. 15 No. 1 February 2026
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v15i1.76562

Abstract

The application of probiotic bacteria in black tiger shrimp (Penaeus monodon) aquaculture constitutes a preventive strategy aimed at mitigating the risk of disease outbreaks. This study aims to compare the efficacy of Bacillus subtilis and Lactobacillus acidophilus in inhibiting Vibrio parahaemolyticus infection through both in vitro and in vivo evaluations. The research was conducted from January to March 2025 at the Fisheries Biology Laboratory, Faculty of Fisheries and Marine Science, Universitas Borneo Tarakan. This study used a Completely Randomized Design (CRD) with four treatments and three replicates each, namely Control (-) uninfected and without probiotics, Control (+) infected with V. parahaemolyticus at 104 CFU/mL without probiotics, Bs given B. subtilis at 108 CFU/mL and infected with V. parahaemolyticus at 104 CFU/mL, and La given L. acidophilus at 108 CFU/mL and infected with V. parahaemolyticus at 104 CFU/mL. Observed parameters included antibacterial inhibition, survival rate (SR), mortality pattern, mean time to death (MTD), relative percentage survival (RPS), bacterial population, clinical symptoms, and water quality. L. acidophilus demonstrated greater efficacy than B. subtilis in inhibiting V. parahaemolyticus infection in black tiger shrimp. The survival rate of shrimp treated with L. acidophilus reached 69.67%, whereas the group treated with B. subtilis exhibited a survival rate of 43.00%. Overall, the application of L. acidophilus consistently yielded superior survival outcomes compared to B. subtilis.