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All Journal HAYATI Journal of Biosciences Indonesian Journal of Tropical and Infectious Disease Bali Medical and Wellness Journal
Janendra, Putu Sathiya Adi
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Agriculture, Biological Sciences & Forestry Dentistry Earth & Planetary Sciences Health Professions Medicine & Pharmacology Nursing Public Health

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Evaluation of the Effectiveness of Local Food-Based Supplementation (Pemberian Makanan Tambahan) in Improving the Nutritional Status of Underweight Children under Five at a Public Health Center Christiyanti, Ni Ketut Wenny; Janendra, Putu Sathiya Adi; Dwijayantari, Si Ayu Dinda; Purnami, Kadek Ima
Bali Medical and Wellness Journal Vol. 2 No. 2 (2025): Bali Medical and Wellness Journal
Publisher : PT BMW Journal Sejahtera

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71341/bmwj.v2i2.35

Abstract

Background: Malnutrition in children under five is a major public health concern in Indonesia. The government’s Supplementary Feeding Program or Pemberian Makanan Tambahan (PMT) aims to improve nutritional status, but its effectiveness needs further evaluation, especially in areas with persistent undernutrition. Aim: This study aimed to evaluate the effectiveness of the local PMT intervention in improving the nutritional status of undernourished children under five years old. Methods: An experimental one-group pre- and post-test design with a cross-sectional approach was used. The Wilcoxon Signed Rank Test facilitated statistical analysis. The study involved a total sample of 50 respondents who received locally sourced supplementary food over 60 days. Results: The study included 50 respondents, with a majority being male (26 toddlers, 52%) and female (24 toddlers, 48%). The findings revealed a statistically significant improvement (p <0.001) in the children's nutritional status, demonstrating the positive impact of the PMT intervention. Conclusion: Although the PMT program effectively enhanced nutritional status, its success is influenced by factors like adherence to dietary guidelines, parental involvement, and education. Integrating nutrition education and regular health monitoring is crucial to sustain long-term nutritional improvements in children.
Impact of Training on Local Food-Based Supplementary Feeding Knowledge of Posyandu Cadres for Undernutrition Management at Banjar 1 Public Health Center Christiyanti, Ni Ketut Wenny; Dwijayantari, Si Ayu Dinda; Janendra, Putu Sathiya Adi; Purnami, Kadek Ima
Bali Medical and Wellness Journal Vol. 2 No. 2 (2025): Bali Medical and Wellness Journal
Publisher : PT BMW Journal Sejahtera

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71341/bmwj.v2i2.36

Abstract

Background: Malnutrition remains a significant health challenge in Indonesia, especially among vulnerable groups like young children and pregnant women. Improving the skills and knowledge of posyandu cadres in preparing local food-based supplementary feeding (Pemberian Makanan Tambahan/PMT) can help address this issue effectively. Methods: This study used a quasi-experimental design with a one-group pre-test and post-test approach. A group of posyandu cadres was assessed before and after receiving training on making PMT from local food sources. The Wilcoxon Signed Rank test was used to analyze the significance of knowledge changes. Results: A total of 40 primarily female cadres participated, with age distribution showing 40% aged 36–45 years, 20% aged 26–35 years, 37% aged 46–55 years, and 3% aged 55–66 years. The training on local food-based PMT resulted in a significant increase in knowledge (p <0.001). Conclusion: Training on preparing and processing local food-based PMT effectively increased the knowledge of posyandu cadres. This improvement is essential for better management of malnutrition among toddlers and pregnant women in the Banjar 1 Public Health area.
Effect of Fetal Bovine Serum Concentration on Detection and Morphological Identification of Blastocystis hominis in vitro Janendra, Putu Sathiya Adi; Sukarma, Kadek Edy; Sarita, Kadek intan Arta; Maheswari, Kadek Indira; Giri, Made Kurnia Widiastuti; Permasutha, Made Bayu; Pasala, Metamalik
Indonesian Journal of Tropical and Infectious Disease Vol. 13 No. 2 (2025)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v13i2.64829

Abstract

Diarrhea significantly contributes to the high rates of illness and death among young children. Diarrhea can be caused by bacterial infections, viruses, or even parasites. Blastocystis hominis causes parasitic diarrhea, which can be identified by microscopy, culture, and molecular methods. Previous reports have modified the Jones’ culture medium using three different serums, such as human plasma, donkey serum, and horse serum (in Jones’ medium). This research replaces horse serum with fetal bovine serum for detection tests, morphological observation, and diagnosis of B. hominis. The research encompasses five experimental groups, each subjected to varying concentrations of fetal bovine serum: 2%, 10%, 20%, 30%, and 40%. Detection analysis is conducted using the Mc-Nemar test, while the Wilcoxon test is applied to evaluate ordinal data from morphological assessments. Diagnostic tests and metrics such as accuracy, sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) are performed using MedCalc® software. The findings demonstrate that serum concentrations of 2%, 10%, 20%, and 30% produced effective results in detection tests, morphological identification, and diagnostic evaluations of B. hominis, exhibiting high sensitivity, specificity, PPV, NPV, and accuracy. Fetal bovine serum can be used at a concentration of 2% in a Jones’ medium that has been modified. This depends on the results of detection tests, morphology, and diagnosis.
Modification of the Kato-Katz Diagnosis Technique Using “Canang” Flower Waste Extract as a Staining for Ascaris lumbricoides Eggs Maheswari, Kadek Indira; Janendra, Putu Sathiya Adi; Widnyana, I Komang Tri Yasa; Mahayana, Dewa Gede Putra; Pramesti, Komang Kirana Ardhia; Dwisaputra, Indra; Permasutha, Made Bayu; Wiguna, Nyoman Intan Permatahati; Giri, Made Kurnia Widiastuti
HAYATI Journal of Biosciences Vol. 33 No. 1 (2026): January 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.1.71-78

Abstract

Helminthiasis, particularly that caused by Ascaris lumbricoides, is a major global health issue, especially in areas with poor sanitation. One method for preventing and controlling Ascaris lumbricoides infection is through identification via the Kato-Katz diagnostic technique. The use of synthetic dyes, such as methylene blue, in the Kato-Katz method raises concerns for both human health and the environment. In humans, methylene blue causes skin irritation, gastrointestinal issues upon ingestion, and systemic effects. Furthermore, its environmental impact includes reducing light penetration and acting as a toxic component in food chains. An alternative approach involves utilizing post-use offerings from Hindu rituals in Bali, known as canang, which consist of flower components such as Impatiens balsamina L. and Tagetes erecta. The natural dyes found in these flowers serve as an alternative to traditional staining methods. This study examined the efficacy of flower extracts as stainings using the cellophane absorption test, helminth egg detection and morphology identification, pH test, and measurement of heavy metal concentration. The dye made from canang flower waste at 3% did not differ much from manufactured stainings. Thus, canang flower waste is a safe alternative.
Co-Authors Christiyanti, Ni Ketut Wenny Dwijayantari, Si Ayu Dinda Indra Dwisaputra Made Bayu Permasutha Made Kurnia Widiastuti Giri Mahayana, Dewa Gede Putra Maheswari, Kadek Indira Pasala, Metamalik Pramesti, Komang Kirana Ardhia Purnami, Kadek Ima Sarita, Kadek intan Arta Sukarma, Kadek Edy Widnyana, I Komang Tri Yasa Wiguna, Nyoman Intan Permatahati