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All Journal Jurnal Biologi Tropis
Sa'uddah, Lousanja D.
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Qualitative Analysis of Partial 16S rRNA Amplicon of Mitochondrial Gene of Stingless Bees in Pesawaran Priyambodo, Priyambodo; Rustiati, Elly Lestari; Pratiwi, Dian Neli; Susanto, Alvin Wiwiet; Imtitsal, Aulia; Fahrezi, Aditya; Febriansyah, Muhammad; Kusuma, Andriyani Wijaya; Srihanto, Eko Agus; Saswiyanti, Enny; Sidik, Mahfud; Sa'uddah, Lousanja D.; Lestari, Indah Ayu; Yani, Ani Andri; Ramadhan, Viki
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.6470

Abstract

Pesawaran is one of the areas that has the potential to cultivate stingless bees as producers of honey and propolis. This study was aimed at qualitatively analysing partial amplicons of the 16S rRNA gene from individuals found at the research site. The research was performed from April to September 2023 in an explorative approach. Based on the exploration that has been done in Harapan Jaya Village, Way Ratai District, Pesawaran Regency, individual stingless bees were obtained from 5 different colonies. Individual samples of stingless bees were further extracted and amplified by commercial kits. Each individual from 5 different colonies has successfully obtained its DNA amplicons. Based on qualitative analysis using 1% agarose gel, the size of the partial gene ranged from 400bp to 500bp. This result is in accordance with the data contained in the genbank, where the 16S rRNA gene size is more than 500bp. Therefore, it has been concluded that the size of the partial amplicons of the 16S rRNA gene of individual stingless bees from the 5 colonies that were obtained is actually in range of the size of the 16S rRNA gene of stingless bees that have been recorded in the genbank.
Co-Authors Eko Agus Srihanto, Eko Agus Elly Lestari Rustiati Fahrezi, Aditya Febriansyah, Muhammad Imtitsal, Aulia Indah Ayu Lestari, Indah Ayu Kusuma, Andriyani Wijaya Pratiwi, Dian Neli Priyambodo Priyambodo Ramadhan, Viki Saswiyanti, Enny Sidik, Mahfud Susanto, Alvin Wiwiet Yani, Ani Andri