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All Journal Journal of Pharmaceuticals and Natural Sciences Jurnal Riseta Naturafarm
Amanda, Rezti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Isolation and Identification of Lactic Acid Bacteria from the Washing Water of Mayas Rice, a Traditional Mountain Rice from East Kalimantan: Isolasi Dan Identifikasi Bakteri Asam Laktat Dari Air Cucian Beras Gunung (Beras Mayas) Khas Kalimantan Timur Amanda, Rezti; Rijai, Laode; Arifuddin, Muhammad
Jurnal Riseta Naturafarm Vol. 2 No. 2 (2025): Jurnal Riseta Naturafarm
Publisher : B-Creta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70392/jrn.v2i2.96104

Abstract

Rice washing water contains carbohydrates, vitamins, and minerals that serve as potential substrates for the growth of Lactic Acid Bacteria (LAB). This study aims to isolate and characterize LAB from the traditional East Kalimantan rice variety Beras Mayas. LAB isolation was performed using De Man Rogosa and Sharpe (MRS) Agar, followed by successive purification to obtain pure cultures. Identification of the isolates involved macroscopic and microscopic observations, Gram staining, and a series of biochemical assays. Three distinct LAB isolates, designated A, B, and C, were obtained. Isolates A and B exhibited circular colonies with yellowish-white pigmentation and undulate margins, suggesting affiliation with the genus Lactobacillus. Isolate C formed circular white colonies with entire margins, indicating a possible relationship with the genus Streptococcus. Biochemical testing revealed that all three isolates were negative for catalase, gelatin hydrolysis, motility, and indole production. In the Triple Sugar Iron Agar (TSIA) test, all isolates fermented glucose, lactose, and sucrose, producing yellow coloration in both the slant and butt portions of the medium. These results suggest that the isolates are homofermentative LAB.
Antibacterial Activity of Lactic Acid Bacteria Isolates Mountain Rice Wash Water (Mayas Rice) Against Propionibacterium acnes Arifuddin, M.; Amanda, Rezti; Arifin, Arfiani; Arifin, Arfina Sukmawati; Iswahyudi, Iswahyudi; Samsul, Erwin; Riki, Riki; Rija'i, Hifdzur Rashif; Hikmawan, Baso Didik; Ibrahim, Arsyik; Rijai, Laode
Journal of Pharmaceuticals and Natural Sciences Vol. 3 No. 1 (2026): J. Pharm. Nat. Sci.
Publisher : B-CRETA Publisher (CV. Borneo Citra Kreatama)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70392/jpns.v3i1.42

Abstract

This study aims to explore the antibacterial activity potential of fermented dragon fruit peel juice (Hylocereus polyrhizus) fermented using Lactic Acid Bacteria (LAB) isolated from rice washing water (Beras Mayas). The process begins with the preparation of dragon fruit peel samples, which are washed, peeled, and mashed to obtain the juice. The juice is pasteurized and inoculated with a 5% LAB starter, with the addition of a 10% sugar solution before being incubated for 3, 7, 12, and 17 days at 37ºC. pH measurements were taken during fermentation, showing a significant decrease in pH, reaching the lowest value of 3.76 on day 17. The antibacterial activity was measured using the well-diffusion method with the fermented juice at a concentration of 100%, showing a very strong inhibition zone (>20 mm) against Propionibacterium acnes on day 7. The results indicate that the pH changes during fermentation are closely related to the antibacterial activity
Co-Authors Arifin, Arfiani Arifin, Arfina Sukmawati Arifuddin, Muhammad Baso Didik Hikmawan Erwin Samsul Ibrahim, Arsyik ISWAHYUDI ISWAHYUDI Laode Rijai M. Arifuddin, M. Rija'i, Hifdzur Rashif RIKI RIKI, RIKI