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All Journal MNJ (Malang Neurology Journal) Ahmar Metastasis Health Journal (AMHJ) Indonesian Community Journal
Rizki Amelia
Poltekkes Kemenkes Kalimantan Timur, Indonesia
Agriculture, Biological Sciences & Forestry Computer Science & IT Dentistry Economics, Econometrics & Finance Environmental Science Health Professions Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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DOES THE COMBINATION OF ROSMARINUS OFFICINALIS AND CENTELLA ASIATICA NANOEMULSIONS AFFECT BDNF EXPRESSION IN THE GESTATIONAL DIABETES MELLITUS MODEL IN ZEBRAFISH LARVAE Khotimah, Husnul; Lamadi, Sutini; Amelia, Rizki; Arista, Devi Maya; Prastiwi, Fajar Dwi; Raras, Tri Yudani Mardining
MNJ (Malang Neurology Journal) Vol. 10 No. 2 (2024): July
Publisher : PERDOSSI (Perhimpunan Dokter Spesialis Saraf Indonesia Cabang Malang) - Indonesian Neurological Association Branch of Malang cooperated with Neurology Residency Program, Faculty of Medicine Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.mnj.2024.010.02.08

Abstract

Background: Pancreatic β-cells compensate for the increased insulin demand due to increasing glucose levels during pregnancy or in gestational diabetes mellitus (GDM). The enzyme that regulates the rate of gluconeogenesis production is PEPCK. Circulatory and metabolic disturbances in the brain caused by hyperglycemia could affect brain-derived neurotrophic factor (BDNF) expression as an agent for neurodevelopment. Objective:  The purpose of this work is to ascertain the impact of combining nanoemulsions of Rosmarinus officinalis and Centella asiatica on the expression of BDNF and PEPCK. Methods: This study was a true experimental laboratory using zebrafish larvae. The larvae were exposed to 3% glucose and a combination of nanoemulsion extracts. The study sample of zebrafish larvae at 72 hpf (hour post fertilization) was divided into control, glucose, treatment 1, 2, and 3, with the combination of Rosmarinus officinalis and Centella asiatica nanoemulsions at 2.5 μg/ml, 5 μg/ml, and 10 μg/ml, respectively. PEPCK and BDNF expression were measured by the real-time PCR method. Results: The expression of PEPCK increased significantly in the glucose group (3.05 ± 0.27). The combination of Rosmarinus officinalis and Centella asiatica nanoemulsions significantly decreased PEPCK (p-value 0,001 < 0.05). The BDNF level did not significantly differ (p-value 0.253 >0.05) among groups, but the highest level (1.06 ± 0.60) was gained in the T2 group, which received an embryonic medium (EM), 3% glucose, and the combination of Rosmarinus officinalis and Centella asiatica nanoemulsions at 5μg/ml. Conclusion: The combination of Rosmarinus officinalis and Centella asiatica nanoemulsions significantly reduces PEPCK expression but does not affect BDNF expression.
The Influence of Video Media on Improving Knowledge and Attitudes of Adolescent Girls Regarding Chronic Energy Deficiency Sari, Riana Trinovita; Wulandari, Nadya; Siregar, Nursyahid; Amelia, Rizki; Supriatin, Supriatin
Ahmar Metastasis Health Journal Vol. 5 No. 1 (2025): Ahmar Metastasis Health Journal
Publisher : Yayasan Ahmad Mansyur Nasirah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/amhj.v5i1.547

Abstract

Adolescent girls are a vulnerable group to nutritional problems, particularly Chronic Energy Deficiency (CED), which can affect reproductive health and the quality of future generations. One of the main contributing factors to CED is the lack of knowledge and attitude toward nutrition. Video media is considered an effective educational tool as it combines engaging visual and audio elements. This study aimed to determine the effect of video media on improving the knowledge and attitudes of adolescent girls regarding CED at SMAN 4 Samarinda. The study employed a pre-experimental one-group pre-test post-test design with a sample of 39 eleventh-grade students selected through purposive sampling. The intervention consisted of a ±10-minute educational video on CED shown twice a week. Data were collected using questionnaires and analyzed using univariate and bivariate analysis with the Wilcoxon test. Results showed that most respondents were 16 years old (64.1%) and had a below-normal Body Mass Index (84.6%), indicating a risk of CED. The most common physical activity was participation in scouting (35.9%), followed by dance and volleyball. After the intervention, there was a significant increase in knowledge, from a median score of 10.00 to 13.00 (p = 0.002), and in attitude, from a median score of 31.00 to 33.00 (p = 0.001). These findings indicate that video media is effective in improving adolescents' understanding of CED. However, its effectiveness may also be influenced by age and prior access to information. Therefore, video media is highly recommended as a nutritional education tool in adolescent health programs.
Peningkatan Pengetahuan dan Keterampilan Ibu Hamil Melalui Edukasi dan Pelatihan Pemantauan Mandiri Kadar Glukosa Darah di Puskesmas Samarinda Kota Amelia, Rizki; Syukur, Nursari Abdul; Wati, Ratna; Puspitaningsih, Rezki; Jasmawati, Jasmawati; Raihanah, Siti
I-Com: Indonesian Community Journal Vol 5 No 4 (2025): I-Com: Indonesian Community Journal (Desember 2025)
Publisher : Fakultas Sains Dan Teknologi, Universitas Raden Rahmat Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70609/i-com.v5i4.8440

Abstract

Rendahnya pengetahuan ibu hamil mengenai Diabetes Melitus Gestasional (DMG) serta belum terlaksananya Pemantauan Mandiri Kadar Glukosa Darah (PMKGD) menjadi permasalahan utama di wilayah Puskesmas Samarinda Kota, meskipun kasus DMG menunjukkan peningkatan dalam tiga tahun terakhir. Kondisi ini menuntut adanya upaya peningkatan literasi kesehatan dan keterampilan ibu hamil melalui edukasi dan pelatihan terstruktur. Kegiatan pengabdian masyarakat ini bertujuan meningkatkan pengetahuan dan keterampilan ibu hamil mengenai DMG dan PMKGD di wilayah Puskesmas Samarinda Kota. Metode pelaksanaan meliputi edukasi partisipatif mengenai DMG dan pelatihan praktik penggunaan glukometer. Evaluasi dilakukan menggunakan pre–post test pengetahuan serta observasi keterampilan. Hasil menunjukkan peningkatan pengetahuan yang signifikan, dengan kategori “baik” meningkat dari 10% menjadi 80%, dan seluruh peserta mampu melakukan PMKGD dengan benar. Kegiatan ini terbukti efektif meningkatkan literasi kesehatan ibu hamil dan mendukung pencegahan komplikasi DMG. Program serupa direkomendasikan untuk diintegrasikan dalam Kelas Ibu Hamil sebagai deteksi dini berkelanjutan.
Co-Authors Arista, Devi Maya Heni Pujiastuti Jasmawati, Jasmawati Lamadi, Sutini Nursari Abdul Syukur Nursyahid Siregar Prastiwi, Fajar Dwi Puspitaningsih, Rezki Ratna Wati Sari, Riana Trinovita Siti Raihanah Supriatin Supriatin, Supriatin Tri Yudani MR Wulandari, Nadya