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All Journal Buletin Veteriner Udayana
I Gusti Ngurah Sudisma
Laboratorium Ilmu Bedah dan Radiologi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia, 80234
Immunology & microbiology Public Health Veterinary

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TREATMENT OF URINARY VESICLE CALCULI IN A FEMALE POMERANIAN DOG Ni Made Wida Rieke Pitaloka; I Gusti Agung Gde Putra Pemayun; I Gusti Ngurah Sudisma
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2025 Vol. 17 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i04.p09

Abstract

Calculi of the urinary vesica is one of the many problems that can occur in pets, especially dogs. This case study aims to find out how to diagnose and treat a case of urinary vesica calculi in a female dog. A female pomeranian dog named Monna, aged 2.4 years with a body weight of 2.7 kg had complaints of difficulty urinating, straining when urinating, and blood in the urine which lasted for approximately 3 months. Supporting examinations were carried out in the form of an ultrasound examination with the discovery of a hyperechoic mass formation in the vesica urinaria suspected of calculi and radiographic examination found a radiopaque image in the vesica urinaria which was believed to be calculi. Based on the results of the supporting examination, the dog was diagnosed with calculi in the vesica urinaria with a prognosis of fausta. The dog was treated with laparocystotomy surgery using a combination of xylazine and ketamine anesthesia intravenously. The calculi found in the case dog showed characteristics of rough, sharp and jagged edges, irregular round shape, with a hard and strong composition, where the calculi found in this case were calculi formed from calcium oxalate. Postoperatively the dog was given Cefotaxime antibiotics (20 mg/kg BW, q12, IV) for 3 days and continued with Cefixime antibiotics (10 mg/kg BW, q12, PO) for 7 days. On the 10th postoperative day, the surgical wound had dried and fused perfectly. The dog had normal activities, normal defecation and urination.
MACROSCOPIC EVALUATION OF INCISION WOUND HEALING IN THE SKIN OF WHITE RATS TREATED WITH PLATELET-RICH PLASMA GEL DERIVED FROM PIG BLOOD Made Amara Sanjiwaning Sukma; I Wayan Wirata; I Wayan Nico Fajar Gunawan; I Gusti Ngurah Sudisma; I Made Kardena
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2025 Vol. 17 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i01.p22

Abstract

Wound healing is the body's attempt to restore its structural integrity and normal function after tissue disruption. Platelet Rich Plasma (PRP) is often used as a regenerative treatment by increasing the activity of growth factors in the blood to treat damaged tissue. Pig blood contains a high concentration of platelets that can support the wound healing process. The purpose of this study was to determine the macroscopic picture of incision wound healing on the skin of white rats given PRP gel from pig blood. The experimental animals used in this study were 27 wistar strain rats. There were three treatments carried out, namely the negative treatment group given 0.9% NaCl solution, the positive control group given bioplacenton, and the treatment group with pig blood PRP gel. Parameters used to determine the level of wound healing include wound length, wound color, wound moisture, and wound scab. Wound length data was analyzed using one-way analysis of variance (Anova). Meanwhile, data on wound color, wound scab, and wound moisture were analyzed by Kruskal Walls. The pig blood PRP gel treatment group had a significant effect on the incision wound healing rate than the negative and positive treatment groups (bioplacenton). PRP gel from pig blood can accelerate wound healing because it contains many growth factors that play an important role in tissue repair and regeneration. Further research is recommended to evaluate the effect of PRP on other types of wounds, such as burns or infectious wounds, as well as developing PRP preservation methods in other forms for wider clinical applications.
HISTOPATHOLOGICAL ANALYSIS OF INCISION WOUND HEALING IN RATS TREATED WITH PIG BLOOD-DERIVED PLATELET-RICH PLASMA GEL Stevanny Lastra; I Wayan Wirata; Ni Luh Eka Setiasih; I Gusti Ngurah Sudisma; I Wayan Gorda; I Made Kardena
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2025 Vol. 17 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i01.p23

Abstract

Platelet Rich Plasma (PRP) can be used as a regenerative treatment to enhance the activity of growth factors in the blood with the aim of wound healing. PRP can enhance neovascularization, fibroblast formation, and tissue epithelialization more quickly and efficiently. This study aims to determine the histopathological observation of incision wound healing on the skin of white rats given PRP gel. This study used male white rats of the Wistar strain, aged 2-2.5 months and weighing 200-300 grams. The 27 rats used were divided into three treatment groups: P0 (negative control, given 0.9% NaCl solution), P1 (positive control, given Bioplacenton), and P2 (given PRP Gel). The treatment was administered once after the skin had been incised and was given only once. On days 1, 5, and 11, a biopsy of the skin organ was performed for histopathological examination. Histopathological examination includes four indicators: inflammatory cell infiltration, angiogenesis, fibroblasts, and collagen density. Data were analyzed using the Kruskal-Wallis test followed by the Mann-Whitney test, and then described descriptively. The research results show that the infiltration of inflammatory cells and collagen density indicate a difference (P≤0,05) in the group of receiving PRP gel compared to the negative and positive control groups. However, there was no difference in angiogenesis and fibroblasts (P>0.05). In the wound healing process, the histopathological picture of incisional wound healing in the skin of white rats (Rattus norvegicus) given pig blood PRP gel shows an increase and development. Therefore, further research can be conducted to create a more optimal PRP gel formulation, and histopathological examinations can be carried out over a longer observation period to obtain significant results.
HISTOPATHOLOGICAL ANALYSIS OF INCISION WOUND HEALING IN RATS GIVEN PLATELET RICH PLASMA DROPS FROM PIG BLOOD Anak Agung Ngurah Hery Brian Priantara; I Wayan Wirata; I Ketut Berata; I Gusti Ngurah Sudisma; I Wayan Gorda Sudisma; Ni Luh Eka Setiasih
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2025 Vol. 17 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i02.p09

Abstract

Platelet Rich Plasma (PRP) is platelet-rich plasma derived from whole blood. Platelet Rich Plasma (PRP) contains growth factors such as transforming growth factor-beta (TGF-β), insulin like growth factor (IGF-I), platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF) and epidermal growth factor (EGF) which play a role in accelerating wound healing. This study aims to determine the histopathological analysis of incision wound healing of white rats given pig blood PRP drops. This study used white rats of male sex with the age of 2 - 2.5 months with a body weight of 200 - 300 g. The rats used in this study were 27 rats divided into two groups. The 27 rats used were divided into 3 treatment groups, namely P0 (negative control, given 0.9% NaCl solution), P1 (positive control, given Bioplacenton), P2 (given PRP drops). On days 1, 5, and 11, biopsies were taken for histopathological examination. Histopathological examination was performed including four indicators: inflammatory cell infiltration, angiogenesis, fibroblasts, and collagen density. Data were then analyzed using Kruskal-Wallis and if there was a significant difference (P<0.05), it would be followed by the Mann Whitney test. From the results of the study on the histopathology of white rat incision wounds given pig blood PRP drops showed an increase in development. On the first day, inflammatory cell infiltration and angiogenesis increased, but decreased on days 5 and 11. Fibroblasts were seen on the first day then decreased until day 11. Collagen on day 1 began to be seen until on day 11 the density of collagen was very tight. It is necessary to make observations in a more detailed period of time to find out more clearly about the effect of PRP drops on angiogenesis.
MANAGEMENT OF ENDOMETRITIS THROUGH OVARIOHYSTERECTOMY IN A FEMALE DOMESTIC CAT I Kadek Ari Satria Prayoga; I Gusti Agung Gde Putra Pemayun; I Gusti Ngurah Sudisma
Buletin Veteriner Udayana Bul. Vet. Udayana. August 2025 Vol. 17 No. 4
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i04.p30

Abstract

Endometritis is inflammation that occurs in the endometrium. Endometritis is caused by post-abortion bacterial infection, dystocia, and retained placenta. This case study aims to find out how to diagnose and treat cases of endometritis in female cats. A white female domestic cat, 7 months old and weighing 2 kg, complained of decreased appetite, weakness, and a thick, milky white discharge from the vagina. Supporting examinations are carried out in the form of routine hematology examinations and ultrasound to support the diagnosis. Routine hematology results showed an increased WBC value and an ultrasound examination showed results showing an enlarged uterus and anechoic-hypoechoic or grayish black fluid. The cat was diagnosed with endometritis with a fausta prognosis. Treatment is carried out by surgical ovariohysterectomy with a combination of intravenous xylazine and ketamine anesthesia. After surgery, cefotaxime sodium (20 mg/kg BW, IV, q12h) was given for 3 days and continued with cefixime (10 mg/kg BW, PO, q12h) for 7 days. On the 12th day the cat was declared cured with the surgical wound completely fused, the suture threads had been removed, and the cat's condition was observed to be healthy with normal appetite and drinking, normal urination and defecation. Endometritis in cats must be treated immediately to prevent further infection from becoming pyometra.
Co-Authors Anak Agung Ngurah Hery Brian Priantara I Gusti Agung Gde Putra Pemayun I Kadek Ari Satria Prayoga I Ketut Berata I Made Kardena I Wayan Gorda I Wayan Gorda Sudisma I Wayan Nico Fajar Gunawan I Wayan Wirata I Wayan Wirata Made Amara Sanjiwaning Sukma Ni Luh Eka Setiasih Ni Luh Eka Setiasih Ni Made Wida Rieke Pitaloka Stevanny Lastra