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All Journal Termometer: Jurnal Ilmiah Ilmu Kesehatan Dan Kedokteran Jurnal Medika: Medika Jurnal Sains, Teknologi dan Kesehatan Science: Indonesian Journal of Science Jurnal Ilmu Kedokteran dan Kesehatan Indonesia
Rachmat Saleh
Universitas Borneo Tarakan
Agriculture, Biological Sciences & Forestry Chemistry Computer Science & IT Dentistry Engineering Health Professions Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health

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POTENSI SENYAWA BIOAKTIF DARI EUCHEUMA COTTONII TERHADAP GEN NS5 DAN ENVELOPE (E) VIRUS DENGUE SERTA TARGET ACHE DAN OBP PADA AEDES AEGYPTI: TINJAUAN MINI Vincentia Ade Rizky; Maria Selviana Joni; Umi Latifah; Ni’mah Hidayatul Laili; Eka Nurul H.P.S; Avinda Sari Kanthi Rahayu; Rachmat Saleh; Nasrullah Tamrin; Awaluddin; Adipura Atmadja Egok; Tri Astuti Sugiyatmi
EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan Vol. 2 No. 4 (2025): EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan, Desember 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/empiris.v2i4.2015

Abstract

Dengue fever remains a major global health concern, especially in tropical regions, due to increasing insecticide resistance and the absence of specific antiviral treatments. Marine-derived natural products, such as those from Eucheuma cottonii, offer promising alternatives. This mini-review aims to evaluate the potential of E. cottonii bioactive compounds targeting molecular markers of Aedes aegypti mosquitoes and the Dengue virus (DENV). A review of 30 relevant national and international articles published between 2015 and 2025 was conducted, focusing on the larvicidal and antiviral activities of E. cottonii. E. cottonii contains flavonoids, alkaloids, terpenoids, and sulfated polysaccharides. These compounds inhibit AChE and OBP enzymes in mosquito larvae and target the NS5 and Envelope (E) proteins of DENV. The antiviral effects are also enhanced by its antioxidant and immunomodulatory activities. Eucheuma cottonii has a significant potential as a multifunctional natural resource for sustainable strategies in dengue control.
Pendampingan Pengelolaan Sampah Berbasis Komunitas Menuju Ecohealthy Campus dan Desa Wisata Sehat Tri Astuti Sugiyatmi; Rachmat Saleh; Nasrullah Tamrin; Eka Nurul Hidayah Puspa Seruni; Awaluddin Awaluddin; Adipura Atmadja Egok; Vincentia Ade Rizky; Avinda Sari Kanthi Rahayu; Maria Selviana Joni; Umi Latifah; Ni'mah Hidayatul Laili
Jurnal Medika: Medika Vol. 5 No. 1 (2026)
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/m5mtm120

Abstract

Permasalahan pengelolaan sampah yang belum optimal masih menjadi isu utama di kawasan pesisir Kota Tarakan, khususnya di Kelurahan Pantai Amal yang berfungsi sebagai kawasan wisata sekaligus lokasi Kampus Universitas Borneo Tarakan. Wilayah ini juga merupakan sentra budidaya rumput laut, di mana penggunaan botol plastik sebagai pelampung berkontribusi terhadap timbulan sampah pesisir yang berdampak pada kesehatan lingkungan. Kegiatan pengabdian kepada masyarakat ini bertujuan meningkatkan pengetahuan, kesadaran, dan komitmen sosial petugas kebersihan Universitas Borneo Tarakan serta anggota Kelompok Swadaya Masyarakat (KSM) Tempat Pengolahan Sampah (TPS) 3R (Reduce, Reuse, Recycle) Amal Jaya dalam pengelolaan sampah berbasis prinsip 3R dan kesehatan lingkungan. Metode yang digunakan adalah pendampingan komunitas melalui penyuluhan interaktif, diskusi, pemberian media edukasi, serta demonstrasi pembuatan komposter metode Takakura. Evaluasi dilakukan secara deskriptif menggunakan pre-test dan post-test serta observasi partisipasi peserta. Kegiatan dilaksanakan dalam dua tahap pada 30 September dan 7 Oktober 2025. Hasil kegiatan menunjukkan tingkat pengetahuan peserta berada pada kategori tinggi, disertai peningkatan pemahaman dan keterlibatan aktif. Kegiatan ini menghasilkan komitmen kolektif untuk melanjutkan pendampingan KSM TPS 3R guna mendukung terwujudnya Ecohealthy Campus dan Desa Wisata Sehat Pantai Amal.
Analisis Epidemiologi Spasio-Temporal Infeksi Dengue Tahun 2016–2025 di Tarakan Tri Astuti Sugiyatmi; Vincentia Ade Rizky; Maria Selviana Joni; Rachmat Saleh; Umi Latifah
Termometer: Jurnal Ilmiah Ilmu Kesehatan dan Kedokteran Vol. 4 No. 3 (2026): Juli : Termometer: Jurnal Ilmiah Ilmu Kesehatan dan Kedokteran
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/termometer.v4i3.6688

Abstract

Dengue Hemorrhagic Fever (DHF) remains a serious public health problem in Tarakan City, Indonesia, with case numbers fluctuating annually. This study aimed to analyze trends in DHF incidence and map the spatial distribution of cases and the Larval-Free Index (LFI) in Tarakan City from 2016 to 2025. A descriptive observational study with a retrospective approach was conducted using secondary data obtained from the Tarakan City Health Department. The variables analyzed included the number of DHF cases, incidence rate (IR), case fatality rate (CFR), endemicity status of subdistricts, and the 2025 LFI. Descriptive analysis and Geographic Information System (GIS)-based spatial mapping were performed. The results showed fluctuating trends in DHF incidence throughout the study period. In 2025, 90 cases were reported, with an IR of 35.2 per 100,000 population and a CFR of 1.11%. Most cases occurred in endemic areas. Spatial analysis revealed an uneven distribution of cases, with some urban villages showing lower LFI values but higher dengue incidence. These findings indicate that GIS-based mapping can support the identification of priority areas for surveillance, vector control, and sustainable larval source reduction programs.
Efektivitas E-Modul Berbasis Video Terhadap Peningkatan Keterampilan Klinis Mahasiswa Pada Pembelajaran Skill Laboratory Ni'mah Hidayatul Laili; Avinda Sari Kanthi Rahayu; Eka Nurul HPS; Vincentia Ade Rizky; Maria Selviana Joni; Umi Latifah; Rachmat Saleh; Awaluddin Awaluddin; Nasrullah Tamrin; Adipura Atmadja Egok; Tri Astuti Sugiyatmi
Science: Indonesian Journal of Science Vol. 2 No. 5 (2026)
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/sciencejurnalkesehatanmasyarakatdanteknologiterapan.v2i5.403

Abstract

Pembelajaran keterampilan klinis pada mahasiswa kedokteran merupakan komponen penting dalam membentuk kompetensi profesional yang terstandar. Proses pembelajaran di skill laboratory sering menghadapi kendala, seperti keterbatasan waktu praktik, variasi kemampuan mahasiswa, serta kurangnya media pembelajaran yang dapat diakses secara fleksibel. . Pembelajaran keterampilan klinis di skill laboratory membutuhkan media yang efektif untuk menunjang peningkatan kemampuan mahasiswa. Penelitian ini bertujuan untuk menganalisis efektivitas e-modul berbasis video terhadap peningkatan keterampilan klinis mahasiswa. Metode penelitian menggunakan desain kuasi-eksperimen dengan pendekatan one group pre-test dan post-test design melalui kuesioner yang diberikan sebelum dan sesudah penggunaan e-modul pada mahasiswa. Data dianalisis secara deskriptif dengan membandingkan hasil sebelum dan setelah intervensi. Hasil menunjukkan adanya peningkatan pemahaman materi, keterampilan prosedural, serta kepercayaan diri mahasiswa dalam melakukan tindakan klinis. Sebagian besar responden juga memberikan penilaian positif terhadap kemudahan penggunaan, kejelasan materi, dan fleksibilitas akses e-modul berbasis video. Kesimpulan penelitian ini adalah e-modul berbasis video efektif dalam meningkatkan keterampilan klinis mahasiswa dan layak digunakan sebagai media pembelajaran pendukung pada kegiatan skill laboratory.
Pemanfaatan Daun dan Bunga Senduduk (Melastoma malabathricum L.) sebagai Pewarna Alami Alternatif Pengganti Eosin 2% pada Identifikasi Telur Trichuris trichiura Umi Latifah; Vincentia Ade Rizky; Maria Selviana Joni; Rachmat Saleh; Nasrullah Tamrin; Adipura Atmadja Egok; Awaluddin Awaluddin
Jurnal Ilmu Kedokteran dan Kesehatan Indonesia Vol. 6 No. 2 (2026): Juli: Jurnal Ilmu Kedokteran dan Kesehatan Indonesia
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jikki.v6i2.11216

Abstract

Examination of helminth eggs in fecal samples remains the primary method for diagnosing helminth infections. A 2% eosin stain is commonly applied to improve contrast in microscopic preparations; however, synthetic dyes have limitations due to their chemical properties and low environmental friendliness. The senduduk plant (Melastoma malabathricum L.) contains natural pigments such as anthocyanins and flavonoids, which may serve as potential natural staining agents. This study aimed to evaluate the effectiveness of senduduk leaf and flower extracts as alternative stains to replace 2% eosin in identifying Trichuris trichiura eggs. This research used a laboratory experimental design consisting of one control group and five treatment groups. The treatments included flower extract with a flower-to-distilled water ratio of (1:5), pure flower extract (100%), flower extract with 70% alcohol (1:5), leaf extract with distilled water (5:1), and leaf extract with 70% alcohol (5:1). The results showed that all extract preparations were able to stain the microscopic slides, enabling clear observation of T. trichiura egg morphology. Extracts using alcohol as a solvent produced stronger and clearer contrast compared to those prepared with distilled water. In conclusion, senduduk leaf and flower extracts demonstrate potential as environmentally friendly natural alternative stains for parasitological microscopic examinations.
Co-Authors Adipura Atmadja Egok Avinda Sari Kanthi Rahayu Awaluddin Awaluddin Awaluddin Eka Nurul H.P.S Eka Nurul HPS Laili, Ni'mah Hidayatul Maria Selviana Joni Nasrullah Tamrin Ni'mah Hidayatul Laili Tri Astuti Sugiyatmi Umi Latifah Vincentia Ade Rizky