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All Journal Biology, Medicine, & Natural Product Chemistry Jurnal Penelitian Pendidikan IPA (JPPIPA) Journal Evaluation in Education (JEE)
Manap Trianto
Tadulako University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Social Sciences Other

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The Impact of Problem Based Learning with Mind Mapping on Learning Outcomes in Ecosystem Lesson Mohammad Jamhari; Yulia Windarsih; Manap Trianto
Jurnal Penelitian Pendidikan IPA Vol 11 No 7 (2025): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i7.11284

Abstract

Education plays a critical role in shaping the cognitive, affective, and psychomotor development of students. In the 21st century, students are expected not only to acquire knowledge but also to develop the ability to think critically, solve problems, and apply concepts in real-life situations. For this reason, this study aims to determine the effect of applying the problem-based learning model assisted by mind mapping on student learning outcomes in ecosystem lesson. This research employed a quasi-experimental approach using the Pretest-Posttest Non-Equivalent Control Group Design, involving both control and experimental classes. The study population included all tenth-grade students at SMA Negeri 1 Biau. Through purposive sampling, four classes were selected, totaling 121 students. Learning outcomes data were collected using a test instrument comprising 30 multiple-choice questions. The data were analyzed using the Mann-Whitney statistical test at a significance level of 0.05. The analysis results revealed that the use of the problem-based learning model combined with mind mapping had a statistically significant impact on student learning outcomes, with a p-value less than 0.05 (0.000 < 0.05). Therefore, it can be concluded that the integration of mind mapping within the problem-based learning model has a positive influence on learning achievement. These findings suggest that this instructional approach is effective and can be implemented in lessons focused on ecosystem.
The Effect of Seaweed Soy Tempe Extract on the Reduction of Blood Sugar Levels in Alloxan Induced Rats (Mus musculus) Abdul Hakim Laenggeng; Asriani Hasanuddin; Sitti Nuryanti; Manap Trianto
Jurnal Penelitian Pendidikan IPA Vol 11 No 7 (2025): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i7.11307

Abstract

Diabetes mellitus is a metabolic disorder characterized by chronic hyperglycemia due to impaired insulin secretion or action. This study aimed to examine the antihyperglycemic effect of seaweed soybean tempeh extract in alloxan-induced diabetic mice. A Completely Randomized Design (CRD) was used with 20 healthy male mice (2–3 months old, 25–30 g), divided into five groups: negative control, positive control (metformin), and three treatment groups receiving extract doses of 25, 75, and 125 mg/kg BW. Prior to treatment, mice underwent acclimatization to ensure uniform baseline conditions. Blood glucose levels were measured to assess the effects of the extract. Data were analyzed using ANOVA followed by the Least Significant Difference (LSD) test. The results showed that alloxan effectively induced hyperglycemia by damaging pancreatic beta cells. Administration of seaweed soybean tempeh extract significantly reduced blood glucose levels (p < 0.05). The highest dose (125 mg/kg BW) produced the most notable effect, comparable to metformin, while the 75 mg/kg BW dose also demonstrated significant efficacy. Both were significantly more effective than the lowest dose. In conclusion, seaweed soybean tempeh extract has potential as an alternative antihyperglycemic agent, especially at higher doses.
Diversity of Insect Visitors to Oil Palm Flowers in Smallholder Plantations as a Potential Resource for Environment Based Learning in Education Samsurizal M Suleman; Manap Trianto; Syech Zainal
Journal Evaluation in Education (JEE) Vol 6 No 3 (2025): July
Publisher : Cahaya Ilmu Cendekia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37251/jee.v6i3.1737

Abstract

Purpose of the study: Insect visitors to oil palm flowers, an important component in the pollination process and ecosystem services, are evaluated for their diversity in this study. The research explores their potential as a resource for environment-based learning rooted in local contexts within smallholder plantations, aiming to raise awareness and support sustainable education practices in the community. Methodology: Insects were collected using sweep nets, yellow pan traps, and direct observation. Identification was conducted using a Nikon SMZ445 stereo microscope and reference to entomological keys. Data analysis was carried out descriptively, focusing on species composition and abundance. An e-pocket book was developed as a learning resource. Main Findings: The results showed a total of 50 morphospecies of insect visitors on both male and female oil palm flowers. The number of insect species visiting male flowers was higher, consisting of 38 morphospecies belonging to 21 families and 10 orders. The results of the study were developed into a local wisdom-based pocket book focused on the diversity of insect visitors to oil palm flowers in smallholder plantations. The learning resource was validated by content experts, design experts, media experts, and students, with an average score of 81.7%, indicating it is highly suitable for use in environment-based education. Novelty/Originality of this study: This study highlights insect diversity in smallholder oil palm plantations, which is rarely documented. It also links ecological data with educational applications, providing a model for utilizing local biodiversity as a teaching resource in environmental and biology education.
Diversity and Composition of Insect Species Associated with Natural Rattan Habitats in Namo Village, Kulawi, Central Sulawesi Musdalifah Nurdin; Samsurizal M Suleman; Syech Zainal; Moh Sabran; Raya Agni; Manap Trianto
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1289-1295

Abstract

Tropical forest ecosystems are home to diverse insect communities that play essential ecological roles, including pollination, decomposition, and nutrient cycling. Among the key forest plants, rattan (Arecaceae) provides both ecological and economic benefits and serves as an important microhabitat for various insect species. This study aimed to identify and analyze the diversity of insect species associated with natural rattan habitats in Namo Village, Kulawi District, Central Sulawesi, Indonesia. Insect sampling was carried out using sweep nets, pitfall traps, and yellow pan traps along rattan-dominated forest transects. A total of 125 individual insects were collected, representing 7 orders, 28 families, and 37 species. The order Hymenoptera showed the highest abundance (41.6%), dominated by Formicidae and Apidae families, followed by Coleoptera (25.6%) and Diptera (17.6%). The Shannon–Wiener Diversity Index (H’ = 2.84) indicated moderate species diversity with high evenness (E = 0.83). Functionally, the insect community consisted of pollinators (38.2%), herbivores (25.4%), decomposers (19.6%), and predators (16.8%). These findings demonstrate that natural rattan ecosystems support diverse and functionally balanced insect assemblages, reflecting stable ecological conditions and minimal anthropogenic disturbance. Conserving natural rattan stands is therefore crucial for maintaining biodiversity and sustaining ecological functions such as pollination and decomposition within tropical forest ecosystems.
Analysis of Nutritional Composition and Antioxidant Activity of Yogurt Enriched with Butterfly Pea (Clitoria ternatea L.) Extract Yulia Windarsih; I Made Budiarsa; Manap Trianto; Akram Akram; Abdul Ashari
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.831-841

Abstract

The growing demand for functional foods has encouraged the development of dairy products enriched with natural bioactive ingredients to improve nutritional value and health benefits. This study aimed to analyze the nutritional composition and antioxidant activity of yogurt enriched with butterfly pea (Clitoria ternatea L.) extract. Yogurt was prepared from fresh goat milk with varying concentrations of butterfly pea extract and evaluated for protein, fat, carbohydrate, moisture, ash, vitamin C contents, antioxidant activity, and sensory acceptance. Proximate analysis, UV–Vis spectrophotometry, and the DPPH method were used for chemical determinations, while a hedonic test assessed color, taste, aroma, and texture. Results showed that increasing butterfly pea extract significantly decreased protein and fat contents but increased vitamin C and antioxidant activity, with the highest values observed in yogurt containing 60% extract and 4% formulation (N60K2). Moisture and ash contents were not significantly affected. Sensory evaluation indicated that yogurt with 60% extract and 2% formulation (N60K1) was preferred regarding color, taste, aroma, and texture. Considering compliance with the Indonesian National Standard (SNI) and consumer acceptance, N60K1 was the optimal formulation. The findings suggest that butterfly pea extract can enhance the functional properties of yogurt, particularly its antioxidant potential, while maintaining acceptable sensory quality. This provides insights for developing functional dairy products with natural plant-based bioactives.
Molecular Identification of Lepidopteran Predators of Coccidae on Coffee Plants (Coffea canephora) in Sigi Regency I Made Budiarsa; Manap Trianto; Bahana Aditya Adnan
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.983-992

Abstract

This study aimed to identify the species of Lepidopteran predators that prey on soft scale insects (Coccidae) found on coffee plants in Sigi Regency using molecular techniques. DNA was extracted using the modified CTAB method (Doyle & Doyle, 1990). PCR amplification targeted the COI gene using universal primers LCO1490 and HCO2198 with a GeneAmp PCR System 9700. Visualization was done using 1% agarose gel and UV-transilluminator. Sequencing was conducted externally. Data were analyzed using BioEdit 7.2.5, BLAST, BOLD Systems, and MEGA11 software. The DNA sequence of the predator sample showed 97.91% similarity to Autoba rubra based on GenBank and 97.59% in BOLD Systems. Phylogenetic analysis confirmed a close genetic relationship with A. rubra, distinct from Eublemma. Morphological similarities with Eublemma were misleading, highlighting the accuracy of molecular identification. This study is the first to confirm the identity of a Lepidopteran predator of coffee scale insects in Indonesia using molecular techniques, revealing its close relation to Autoba rubra. The results correct previous morphological misidentifications and contribute new data on predator diversity in biological control contexts.
The Effectiveness of Honey Purity Testing from Several Regions in Central Sulawesi Province I Made Budiarsa; Manap Trianto; Akram Akram; Yulia Windarsih; Abdul Ashari
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1231-1240

Abstract

Honey is a valuable natural product with nutritional, medicinal, and economic importance, but its high commercial value makes it prone to adulteration, which threatens consumer trust and product quality. This study aimed to evaluate the effectiveness of simple purity tests in identifying adulteration in honey samples collected from several regions in Central Sulawesi Province. A total of 12 honey samples from different bee species and floral sources were analyzed using seven tests, namely solubility, cloudiness and foam, heating, hexagonal pattern, seepage, raw fish, and onion tests. The results showed that most tests successfully distinguished pure honey from adulterated samples. Specifically, the solubility, cloudiness and foam tests, as well as heating, and raw fish tests indicated that 66.67% of the samples were pure, while the hexagonal pattern, seepage, and onion tests confirmed purity in all samples (100%). These findings demonstrate that simple, low-cost methods can provide reliable initial screening of honey purity and are suitable for routine use in local communities where advanced laboratory facilities are limited. The application of these tests is expected to support honey quality assurance, protect consumer trust, and promote the sustainable development of the beekeeping industry in Central Sulawesi.
Major Royal Jelly Protein 2 (mrjp2) Gene in Apis cerana from Central Sulawesi, Indonesia I Made Budiarsa; Manap Trianto; Akram Akram; Yulia Windarsih; Abdul Ashari
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.39-44

Abstract

Indonesia is recognized as one of the world’s biodiversity hotspots, supporting a wide range of pollinating insects, including honey bee species of the genus Apis. Among these, Apis cerana is the most widely managed species and contributes significantly to national honey production, particularly in Sulawesi. However, the increasing economic value of honey has led to frequent cases of entomological origin fraud, in which honey is falsely labeled as being produced by a different bee species. This study aimed to determine the entomological origin of honey sold in Central Sulawesi using the Major Royal Jelly Protein 2 (mrjp2) gene as a molecular marker. Seven honey samples claimed as “forest honey” were analyzed through DNA extraction, PCR amplification using species-specific primers (CF-CR), and sequencing followed by BLAST-n analysis. All samples yielded high-quality DNA with purity ratios appropriate for PCR amplification. PCR successfully amplified the mrjp2 gene, producing fragments of approximately 201-208 bp, consistent with the expected size for A. cerana. Sequence analysis showed percent identity values ranging from 99.20% to 100% when compared with reference sequences of A. cerana. Based on molecular verification, three samples (H3, H5, and H7) did not match the seller’s claims and were confirmed to originate from A. cerana rather than A. dorsata binghami. These findings demonstrate that sequencing of the mrjp2 gene is an effective and reliable approach to authenticate the entomological origin of honey and to detect fraudulent labeling in commercial honey products.
Co-Authors Abdul Ashari Abdul Hakim Laenggeng Akram Akram Asriani Hasanuddin Bahana Aditya Adnan I Made Budiarsa Moh Sabran Mohammad Jamhari Musdalifah Nurdin Raya Agni Samsurizal M Suleman Sitti Nuryanti Syech Zainal Yulia Windarsih