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All Journal Bioscience AL KAUNIYAH Biosfer: Jurnal Tadris Biologi Jurnal Penelitian Pendidikan IPA (JPPIPA) JSFK (Jurnal Sains Farmasi & Klinis) PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Pelita Eksakta Jurnal Biogenerasi Jurnal Sains dan Teknologi Journal of Tropical Pharmacy and Chemistry
Fadilaturahmah Fadilaturahmah
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Negeri Padang, Padang, 25131, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Transportation Other

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Isolasi dan Pemurnian DNA Genomik dari Sampel Darah Manusia Menggunakan Metode Spin Column Nuri Febriani; fadilaturahmah fadilaturahmah; Desi Arisanty
Jurnal Sains Dan Teknologi | E-ISSN : 3063-9980 Vol. 2 No. 4 (2026): April - Juni
Publisher : GLOBAL SCIENTS PUBLISHER

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Abstract

Genomic DNA isolation is a crucial step in various molecular biology analyses because the quality of the DNA obtained is crucial for the success of subsequent analyses. This study aims to describe the process of isolating and purifying genomic DNA from human blood samples using the spin column method and to evaluate the quality of the resulting DNA. The study was conducted at the Biomedical Laboratory, Faculty of Medicine, Andalas University, Padang, West Sumatra. The isolation process was carried out using a silica column-based DNA extraction kit, which includes the stages of cell lysis, DNA binding to a silica membrane, washing, and elution. The quality of the extracted DNA was evaluated based on its concentration and absorbance ratio A260/A280 using a microvolume spectrophotometer. The results showed that most samples had an A260/A280 ratio in the range of 1.80–1.90, indicating DNA with a good level of purity and relatively free from protein contamination. The obtained DNA concentration varied between 1.4–92.2 ng/µl, reflecting differences in extraction efficiency in each sample. The spin column method has proven effective in producing genomic DNA with adequate quality and purity for advanced molecular biology applications, such as PCR, electrophoresis, and sequencing. With its fast, safe, and reproducible procedure, this method has become one of the most widely used techniques in diagnostic laboratories and molecular research.
The Potential of Acorus calamus L. in The Ethnobotany of The Malay Ethnic Communities in Riau Province Wahyuni, Sri; Herru, Yoan De Nanda; Chahyadi, Ennie; Afidah, Mar’atul; Fadilaturahmah
Biosfer: Jurnal Tadris Biologi Vol 17 No 1 (2026): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v17i1.31023

Abstract

Acorus calamus L. is a medicinal plant traditionally used by Malay ethnic communities in Riau Province, Indonesia. However, studies integrating ethnobotanical knowledge with phytochemical evidence are still limited. This study aimed to examine the traditional utilization of Acorus calamus and identify its secondary metabolites as scientific support for its medicinal use. A mixed-method approach was applied by combining qualitative ethnobotanical inquiry with experimental phytochemical screening. Ethnobotanical data were collected through interviews, observation, and documentation involving a traditional healer in Pengambang Jaya Village, Riau Province. Phytochemical analysis was conducted on methanolic extracts of the rhizome using standard qualitative screening methods. The results showed that the rhizome of Acorus calamus is mainly used as a traditional remedy for fever. Phytochemical screening revealed the presence of phenolics, flavonoids, alkaloids, and triterpenoids, while steroids and saponins were not detected. These findings indicate that Acorus calamus contains bioactive compounds associated with medicinal properties and support its traditional use. The study highlights the importance of integrating traditional knowledge with scientific validation in ethnobotanical research. Potensi Acorus calamus L. dalam Etnobotani Komunitas Etnis Melayu di Provinsi Riau ABSTRAK:  Acorus calamus L. merupakan tanaman obat yang secara tradisional dimanfaatkan oleh masyarakat etnis Melayu di Provinsi Riau, Indonesia. Namun, penelitian yang mengintegrasikan pengetahuan etnobotani dengan bukti fitokimia masih terbatas. Penelitian ini bertujuan untuk mengkaji pemanfaatan tradisional Acorus calamus serta mengidentifikasi metabolit sekundernya sebagai dukungan ilmiah terhadap penggunaan obat tradisionalnya. Penelitian menggunakan pendekatan mixed-method dengan menggabungkan kajian etnobotani kualitatif dan uji fitokimia eksperimental. Data etnobotani dikumpulkan melalui wawancara, observasi, dan dokumentasi dengan melibatkan seorang praktisi pengobatan tradisional di Desa Pengambang Jaya, Provinsi Riau. Analisis fitokimia dilakukan pada ekstrak metanol rimpang menggunakan metode skrining kualitatif standar. Hasil penelitian menunjukkan bahwa rimpang Acorus calamus terutama digunakan sebagai obat tradisional untuk mengatasi demam. Uji fitokimia menunjukkan adanya senyawa fenolik , flavonoid, alkaloid, dan triterpenoid, sedangkan steroid dan saponin tidak terdeteksi. Temuan ini menunjukkan bahwa Acorus calamus mengandung senyawa bioaktif yang berpotensi sebagai obat dan mendukung penggunaan tradisionalnya. Penelitian ini menegaskan pentingnya integrasi pengetahuan tradisional dengan validasi ilmiah dalam penelitian etnobotani.
Co-Authors Afidah, Mar’atul Afifatul Achyar Defni Yannita Desi Arisanty Dezi Handayani Dwi Hilda Putri ENNIE CHAHYADI, ENNIE Filza Yulina Ade Firman Syukri IRDAWATI Joni, Rahma Kardiman, Reki Kheniva Diah Anggita Marta Dinata Martala Sari Nuri Febriani oktriani, fazdkia Pratama, Sandi Fransisco Putra Santoso Putri Qalbina Rahmi Kurniati Resti Rahayu Satria, Rijal Sa’diatul Fuadiyah Sri Wahyuni Sulaeman, Nabila Threo Wanda Marten Yoan De Nanda Herru Yona Afriani Yusni Atifah