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Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
JBBI, Indonesian Journal of Biotechnology & Bioscience, is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, as well as life sciences in general. Initiated at the then Biotech Centre, the journal is published by the Laboratory for Biotechnology, the Agency for the Assessment and Application of Technology, BPPT.
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RESPONS PERTUMBUHAN BIBIT KENTANG (Solanum tuberosum) TERHADAP FORMULASI BIOSTIMULAN BERBASIS Trichoderma spp. Nawfetrias, Winda; Handayani, Dwi Pangesti; Bidara, Irna Surya; Tanjung, Armelia
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.958 KB) | DOI: 10.29122/jbbi.v6i2.3710

Abstract

Growth Responses of Potato (Solanum tuberosum) Plantlets upon Application of Different Formulations of Trichoderma spp.-Based BiostimulantPlantlet acclimatization is one of several critical steps in potato clonal seedling production which often hampers the availability of quality plant materials. Application of biostimulant formulation containing Trichoderma spp. may increase growth capability of potato plantlets due to the improvement of plantlets in absorbing nutrient from the growth media. The aim of this research was to obtain the best formulations of biostimulant containing several strains of Trichoderma spp. to be applied in the acclimatization stage of potato seeds. Nine formulations of biostimulant (i.e. S1, S2, S3, S4, S5, S6, S7, S8, S9) each of which contained three to four different strains of five Trichoderma spp. isolates, were mixed with organic substrate carriers. The biostimulants were then applied to potato plantlets cv. Atlantic at the beginning of acclimatization. Plantlets without biostimulant were used as the control treatment (S0). The results showed that the S8 biostimulant formulation significantly increased the growth of the potato plantlets. The results of this experiment indicated that compared with the control and other formulations, the S8 biostimulant formulation significantly increased the growth of potato plantlets based on the number of leaves and number of shoots.Keywords: aclimatitation; biostimulant; potato; seedling; TrichodermaABSTRAKAklimatisasi planlet adalah salah satu tahapan kritis dalam produksi bibit klonal kentang yang seringkali menghambat ketercukupan bahan tanaman berkualitas. Penggunaan produk berbasis Trichoderma spp. diduga dapat meningkatkan kemampuan planlet untuk menyerap unsur hara dari media tumbuh. Tujuan penelitian ini adalah untuk mengetahui formulasi biostimulan terbaik yang mengandung Trichoderma spp. yang diaplikasikan pada tahap aklimatisasi bibit kentang. Sembilan biostimulan (S1, S2, S3, S4, S5, S6, S7, S8, S9) diformulasikan menggunakan substrat pembawa organik dan masing-masing mengandung campuran tiga atau empat strain dari lima isolat Trichoderma spp. Kesembilan biostimulan tersebut kemudian diujikan pada planlet kentang varietas Atlantik siap aklimatisasi. Planlet tanpa aplikasi biostimulan digunakan sebagai kontrol (S0). Hasil percobaan ini menunjukkan bahwa dibandingkan dengan control maupun formulasi lainnya, formulasi biostimulan S8 secara signifikan meningkatkan pertumbuhan planlet kentang cv. Atlantik dilihat dari jumlah daun dan jumlah tunasnya.
IDENTIFIKASI BAKTERI PATOGEN PENYEBAB PENYAKIT PURPLE SYNDROME PADA KARANG FUNGIA DI PULAU HARI SULAWESI TENGGARA Palupi, Ratna Diyah; Sadarun, Baru; Sawonua, Paiga Hanurin
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1443.583 KB) | DOI: 10.29122/jbbi.v6i2.3116

Abstract

IDENTIFIKASI BAKTERI PATOGEN PENYEBAB PENYAKIT PURPLE SYNDROME PADA KARANG FUNGIA DI PULAU HARI SULAWESI TENGGARA Nowadays coral disease is one of the causes of damage to coral reefs in Indonesia. Causative agents were found for some types of coral disease. This study aims to identify the type of pathogenic bacteria that cause purple syndrome which attacks Fungia corals. The study was conducted using descriptive exploratory methods. Corals infected with purple syndrome were collected on Pulau Hari, Southeast Sulawesi, through scuba diving. Then, microbiological analysis was carried out which included isolation using the scatter method, purification using a scratch method, a challenge test (antagonistic), a Koch Postulate test, and DNA analysis of putative bacterial isolates. Results showed that 5 bacterial isolates lived in symbiosis with the corals infected with purple syndrome (PSMH1, PSMH2, PSMH3, PSMH4, and PSMH5). Based on the Koch postulate test, 2 bacterial isolates which were pathogenic were obtained, namely PSHM2 and PSHM4 isolates. These bacteria infected the test corals with the characteristics of coral skeleton damage and coral bleaching (dead). Based on biomolecular testing, the two isolates were members of Enterobacter cloacae with a 99% similarity level.Keywords: Coral disease; Enterobacter cloacae; Fungia coral; Hari island; Purple syndromeABSTRAKSaat ini penyakit karang menjadi salah satu penyebab kerusakan terumbu karang di Indonesia. Penyebab pembawa untuk beberapa jenis penyakit karang sudah ditemukan. Penelitian ini bertujuan untuk mengidentifikasi jenis bakteri patogen penyebab penyakit purple syndrome yang menyerang karang Fungia. Penelitian dilakukan menggunakan metode deskriptif eksploratif. Sampel karang yang terinfeksi purple syndrome diambil di Pulau Hari, Sulawesi Tenggara, melalui scuba diving. Selanjutnya, analisis mikrobiologi dilakukan yang meliputi isolasi menggunakan metode sebar, purifikasi menggunakan metode gores, uji tantang (antagonistik), uji Postulat Koch, dan analisa DNA isolat bakteri yang diduga bersifat patogen. Hasil penelitian menemukan 5 isolat bakteri yang bersimbiosis dengan karang yang terinfeksi penyakit purple syndrome (PSMH1, PSMH2, PSMH3, PSMH4, dan PSMH5). Berdasarkan uji postulat Koch, 2 isolat bakteri yang bersifat patogen didapatkan, yaitu isolat PSHM2 dan PSHM4. Bakteri tersebut menginfeksi karang uji dengan ciri kerusakan skeleton karang dan pemutihan karang (mati). Berdasarkan uji biomolekuler kedua isolat tersebut merupakan anggota Enterobacter cloacae dengan tingkat kemiripan 99%.
KONSENTRASI MALONDIALDEHID PADA TIKUS DIABETIK YANG DIBERI PAKAN BERBAHAN SAGU (Metroxylon sagu) DAN Moringa oleifera Muhajirin, Rahmah Nadea Fitriyani; Fauziyah, A'immatul; Marjan, Avliya Quratul
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1751.818 KB) | DOI: 10.29122/jbbi.v6i2.3630

Abstract

Malondialdehyde Concentration in Diabetic Rats Given Feed Made from Sagu (Metroxylon sagu) and Moringa oleifera ABSTRACTHyperglycemia in type 2 diabetes mellitus (DMT2) can cause oxidative stress characterized by increased malondialdehyde (MDA) production. Flavonoids have antioxidant activity that can suppress the production of free radicals that cause oxidative stress. Flavonoids are found in Cersa mori (CM), a food product made from sagu (Metroxylon sagu) and Moringa oleifera. This study aims to analyze the effect of CM administration on MDA levels in alloxan-induced diabetic rats. Antioxidant activity and total CM flavonoids were analyzed using DPPH reduction and colorimetry methods. Thirty-two Wistar rats were divided into 4 groups: negative control (K1), positive control 1 (K2), positive control 2 (K3) and treatment of feeding 5 g CM/200 g BW per day (K4). The intervention was carried out for 30 days. MDA levels were examined before and after the intervention by spectrophotometry. The results showed a significant decrease in MDA levels in K2, K3, and K4 by -11.5 ± 3.39 μM, -10.5 ± 4.32 μM, and -14.9 ± 2.85 μM, respectively. The lowest decrease in MDA levels was found in K4 fed with CM (p < 0.05).Keywords: alloxan; diabetes; flavonoid; malondialdehyde; resistant starchABSTRAKHiperglikemia pada diabetes melitus tipe 2 (DMT2) dapat menimbulkan stres oksidatif yang ditandai dengan peningkatan produksi malondialdehid (MDA). Flavonoid memiliki aktivitas antioksidan yang mampu menekan produksi radikal bebas penyebab stres oksidatif. Flavonoid terdapat di dalam produk pangan Cersa mori (CM) yang terbuat dari sagu (Metroxylon sagu) dan kelor (Moringa oleifera). Penelitian ini bertujuan menganalisis pengaruh pemberian CM terhadap kadar MDA tikus diabetes yang diinduksi aloksan. Aktivitas antioksidan dan total flavonoid CM masing-masing dianalisis menggunakan metode reduksi DPPH dan kolorimetri.Tiga puluh dua ekor tikus Wistar dibagi menjadi 4 kelompok: kontrol negatif (K1), kontrol positif 1 (K2), kontrol positif 2 (K3), dan perlakuan yang diberikan 5 g CM/200 g BB per hari (K4). Intervensi dilaksanakan selama 30 hari. Kadar MDA diperiksa sebelum dan setelah intervensi secara spektrofotometri. Hasil menunjukkan adanya penurunan signifikan kadar MDA pada K2, K3 dan K4 yaitu secara berturut-turut sebesar -11,5 ± 3,39 µM; -10,5 ± 4,32 µM; dan -14,9 ± 2,85 µM. Penurunan kadar MDA terendah terdapat pada K4 yang diberikan CM (p < 0,05).
KERAGAMAN UBI KAYU (Manihot esculenta Crantz.) HASIL PERBANYAKAN IN VITRO BERDASARKAN KARAKTER MORFOLOGI DAN PENANDA ISSR Hartanti, Fajri; Miftahudin, Miftahudin; Hartati, N Sri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1494.884 KB) | DOI: 10.29122/jbbi.v6i2.3055

Abstract

Morphological and Molecular Diversity of Cassava (Manihot esculenta Crantz) Resulted from In Vitro PropagationIn vitro propagation of cassava (Manihot esculenta Crantz) on medium containing plant growth regulator (PGR) may induce morphological variation. This study aimed to analyze the morphological and genetic diversity of 13 genotypes of cassava resulted from in vitro propagation and stem cuttings based on 11 vegetative characters and 7 ISSR markers. Morphological and genetic characters were scored and used for clustering using NTSYS-pc 2.11a. Roti control and Adira 4 control genotypes that were in vitro propagated without PGR addition showed different morphological characters with Roti variant and FEC-25 genotypes that were in vitro propagated with the addition of PGR. Morphological and molecular characters of 13 genotypes showed high diversity. Clustering analysis based on morphological characters classified the in vitro propagated and control plants into four groups at 45.6% similarity. Clustering analysis based on molecular characters classified the plants into three groups at 66.0% similarity.Keywords: cassava; diversity; in vitro; ISSR; morphologyABSTRAKPerbanyakan tanaman ubi kayu (Manihot esculenta Crantz) secara in vitro menggunakan zat pengatur tumbuh (ZPT) diyakini dapat menginduksi variasi morfologi. Tujuan dari penelitian ini adalah untuk menganalisis keragaman morfologi dan molekuler dari 13 genotipe ubi kayu hasil perbanyakan in vitro dan perbanyakan dengan stek batang berdasarkan 11 karakter vegetatif dan 7 penanda ISSR. Karakter morfologi dan molekuler diskor untuk analisis kelompok menggunakan program NTSYS-pc 2.11a. Genotipe Roti kontrol dan Adira 4 kontrol yang merupakan hasil perbanyakan in vitro tanpa penambahan ZPT menunjukkan perbedaan variasi morfologi dengan genotipe Roti varian dan FEC-25 yang merupakan tanaman hasil perbanyakan dengan penambahan ZPT. Hasil analisis pada 13 genotipe menunjukkan adanya keragaman yang tinggi. Hasil analisis kelompok berdasarkan penanda morfologi memisahkan antara genotipe hasil perbanyakan secara in vitro yang ditambah ZPT dengan tanaman kontrolnya ke dalam 4 kelompok dengan nilai koefisen similaritas 45,6%. Hasil analisis kelompok berdasarkan penanda molekuler memisahkan antara genotipe hasil perbanyakan secara in vitro yang ditambah ZPT dengan tanaman kontrolnya ke dalam 3 kelompok dengan nilai koefisen similaritas 66,0%.
EKSTRAKSI DAN IDENTIFIKASI METABOLIT SEKUNDER DARI ISOLAT AL6 SERTA POTENSINYA SEBAGAI ANTIBAKTERI TERHADAP Escherichia coli Syarifuddin, Alfian; Kamal, Sodiq; Yuliastuti, Fitriana; Pradani, Missya Putri Kurnia; Septianingrum, Ni Made Ayu Nila
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2891.129 KB) | DOI: 10.29122/jbbi.v6i2.3516

Abstract

Extraction and Identification of Secondary Metabolites from AL6 Isolates and Its Potential as Antibacterial against Escherichia coliABSTRACTSecondary metabolites in the form of antibiotics can be produced by rhizospheric bacteria. AL6 bacterial isolate, which is one of the bacterial isolates from the rhosphere of Saccarum officinarum L., is known to produce antibiotic compounds. This study aims to determine the activity of antibiotics from AL6 ethyl acetate extracts produced by AL6 bacterial isolates, to analyze the minimum inhibitory concentration (MIC) and the similarity of the active substances using GCMS. The ethyl acetate extract obtained was tested for MIC at 1.25%, 2.5%, 5.0%, 10.0%, 20%, and 40% concentrations. Detection of potential antibiotic spots was carried out using bioautographic thin layer chromatography (TLC). Compounds responsible for antibiotic activity were analyzed using GCMS. Minimum inhibitory levels obtained reached 2.5%. The active spots responsible for antibiotic activity against Escherichia coli at Rf 0.94. Components detected using GCMS and suspected to be antibiotics include chloroform; ethane, 1,1-dimethoxy-(CAS) dimethyl acetal; dan 1,3-dioxolane, 2-methoxymethyl-2,4,5-trimethyl.Keywords: AL6 bacterial isolate; antibiotic; Escherichia coli; GCMS; MICABSTRAKMetabolit sekunder berupa antibiotik dapat diproduksi oleh bakteri rizosfer. Isolat bakteri AL6, salah satu isolat bakteri dari rizosfer Saccarum officinarum L., diketahui dapat menghasilkan senyawa antibiotik. Penelitian ini bertujuan mengetahui aktivitas antibiotik dari ekstrak etil asetat antibiotik AL6 yang dihasilkan isolat bakteri AL6, menganalisis kadar hambat minimum (KHM), serta kemiripan zat aktif menggunakan GCMS. Ekstrak etil asetat yang diperoleh diuji KHM-nya pada konsentrasi 1,25%, 2,5%, 5,0%, 10,0%, 20%, dan 40%. Deteksi bercak yang berpotensi sebagai antibiotik dilakukan menggunakan kromatografi lapis tipis (KLT) bioautografi. Senyawa yang berperan dalam aktivitas antibiotik dianalisis menggunakan GCMS. Kadar hambat minimal yang diperoleh mencapai 2,5%. Hasil uji KLT bioautografi memperlihatkan bercak aktif sebagai antibiotik terhadap Escherichia coli pada Rf 0,94. Komponen senyawa yang terdeteksi menggunakan GCMS dan diduga sebagai antibiotik antara lain chloroform; ethane, 1,1-dimethoxy-(CAS) dimethyl acetal; dan 1,3-dioxolane, 2-methoxymethyl-2,4,5-trimethyl.
IDENTIFIKASI DAN KARAKTERISASI BAKTERI AMILOLITIK PADA UMBI Colocasia esculenta L. SECARA MORFOLOGI, BIOKIMIA, DAN MOLEKULER Wulandari, Destik; Purwaningsih, Desi
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (656.658 KB) | DOI: 10.29122/jbbi.v6i2.3084

Abstract

Morphological, Biochemical, and Molecular Identification and Characterization of Amylolytic Bacteria in Tubers of Colocasia esculentaTaro tuber (Colocasia esculenta L.) has a high starch content of 77.9% so that it can be used as a substrate from which to isolate amylolytic bacteria. The purpose of this study was to isolate amylolytic bacteria from taro tubers, and subsequently to identify as well as to characterize morphologically, biochemically and molecularly using the 16S rRNA technique. Isolation of amylolytic bacteria was carried out by growing bacterial colonies on starch agar media and then selecting those colonies that had clear zones. Bacteria that produced clear zones were then characterized and identified through Gram staining, spore staining, biochemical test, and 16S rRNA molecular test. Results showed that there were seven positive isolates of amylolytic bacteria namely ECE-1, ECE-2, ECE-3, ECE-4, ECE-5, ECE-6, and ECE-7 isolates. Five isolates were identified using 16S rRNA technique. Identification results showed that the seven isolates obtained were putatively identified as Pseudomonas knackmussii, Bacillus siamensis, Bacillus siamensis, Bacillus subtilis, and Bacillus altitudinis.Keywords: 16S rRNA analysis; amylase enzyme; amylolytic bacteria; amylum; taro tuberABSTRAKUmbi talas (Colocasia esculenta L.) mempunyai kandungan pati tinggi yakni sebesar 77,9% sehingga dapat digunakan sebagai bahan untuk mengisolasi bakteri amilolitik. Tujuan penelitian ini adalah mengisolasi bakteri amilolitik dari umbi talas, dan kemudian mengidentifikasi serta mengkarakterisasi secara morfologi, biokimia dan molekuler menggunakan teknik 16S rRNA. Isolasi bakteri amilolitik dilakukan dengan cara menumbuhkan koloni bakteri pada media starch agar dan selanjutnya memilih koloni yang mempunyai zona bening. Bakteri yang menghasilkan zona bening kemudian dikarakterisasi dan diidentifikasi menggunakan metode pewarnaan Gram, pewarnaan spora, uji biokimia, dan uji molekuler 16S rRNA. Hasil menunjukkan terdapat tujuh isolat positif bakteri amilolitik yakni isolat ECE-1, ECE-2, ECE-3, ECE-4, ECE-5, ECE-6, dan ECE-7. Lima isolat diidentifikasi dengan teknik 16S rRNA. Hasil menunjukkan bahwa ketujuh isolat tersebut masing-masing secara berurutan diduga teridentifikasi sebagai Pseudomonas knackmussii, Bacillus siamensis, Bacillus siamensis, Bacillus subtilis, dan Bacillus altitudinis.
DEKSTROSA MONOHIDRAT KUALITAS FARMASI DARI PATI Manihot ecsulenta, Metroxylon sagu, Zea mays, Oryza sativa, dan Triticum Kartika, Bayu Mahdi; Khojayanti, Lely; Nuha, .; Listiana, Shelvi; Kusumaningrum, Susi; Wijaya, Ayustiyan Futu
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1230.738 KB) | DOI: 10.29122/jbbi.v6i2.3208

Abstract

Pharmaceutical Grade Dextrose Monohydrate from Manihot ecsulenta, Metroxylon sagu, Zea mays, Oryza sativa, dan Triticum Starch ABSTRACT Pharmaceutical-grade dextrose monohydrate, one of raw materials used as active pharmaceutical ingredients (API) and additives, can be made from starch. There are five types of local Indonesian commercial starch that are potentially used, namely tapioca (Manihot esculenta), sago (Metroxylon sagu), corn (Zea mays), rice (Oryza sativa), and wheat (Triticum) starch. This study aimed to compare these five starches as raw materials for preparing pharmaceutical-grade dextrose monohydrate which was expected to meet the requirements of the Indonesian Pharmacopoeia (5th Edition) and the United States Pharmacopeia (USP). The starch was converted into dextrose monohydrate through liquefaction hydrolysis, saccharification hydrolysis, activated carbon purification and filtration, ion exchange purification, evaporation, crystallization and drying.  High Performance Liquid Chromatogram (HPLC) and the Luff-Schoorl methods were used for dextrose equivalent value (DE) analysis. The results showed that only three of the starch types produced pharmaceutical-grade dextrose monohydrate, namely (DE) sago starch (107.23% and 100.77%), corn starch (97.86% and 96.19%), and tapioca starch (85.18% and 99.20%).Keywords: dextrose equivalent, dextrose monohydrate, hydrolysis, pharmaceutical grade, starchABSTRAKDekstrosa monohidrat kualitas farmasi, salah satu bahan baku yang digunakan sebagai active pharmaceutical ingredient (API) dan bahan tambahan, dapat dibuat dari bahan pati-patian. Terdapat lima jenis pati komersial lokal Indonesia yang berpotensi digunakan yakni pati tapioka (Manihot esculenta), pati sagu (Metroxylon sagu), pati jagung (Zea mays), pati beras (Oryza sativa), dan pati gandum (Triticum). Penelitian ini bertujuan membandingkan lima jenis pati tersebut sebagai bahan baku pembuatan dekstrosa monohidrat kualitas farmasi yang diharapkan mampu memenuhi standar persyaratan dari Farmakope Indonesia Edisi V dan United States Pharmacopeia (USP). Pati diubah menjadi dekstrosa monohidrat melalui hidrolisis likuifikasi, hidrolisis sakarifikasi, pemurnian karbon aktif dan filtrasi, pemurnian ion exchange, evaporasi, kristalisasi dan pengeringan. Metode High Performance Liquid Chromatogram (HPLC) dan Luff-Schoorl digunakan untuk analisis dextrose equivalent (DE). Hasil penelitian menunjukkan hanya tiga jenis pati yang menghasilkan dekstrosa monohidrat kualitas farmasi, yakni (DE) pati sagu (107,23% dan 100,77%), pati jagung (97,86% dan 96,19%), dan pati tapioka (85,18% dan 99,20%).Kata kunci: dekstrosa monohidrat, dextrose ekuivalen, hidrolisis, kualitas farmasi, pati
KERAGAMAN UBI KAYU (Manihot esculenta Crantz.) HASIL PERBANYAKAN IN VITRO BERDASARKAN KARAKTER MORFOLOGI DAN PENANDA ISSR Fajri Hartanti; Miftahudin Miftahudin; N Sri Hartati
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1494.884 KB) | DOI: 10.29122/jbbi.v6i2.3055

Abstract

Morphological and Molecular Diversity of Cassava (Manihot esculenta Crantz) Resulted from In Vitro PropagationIn vitro propagation of cassava (Manihot esculenta Crantz) on medium containing plant growth regulator (PGR) may induce morphological variation. This study aimed to analyze the morphological and genetic diversity of 13 genotypes of cassava resulted from in vitro propagation and stem cuttings based on 11 vegetative characters and 7 ISSR markers. Morphological and genetic characters were scored and used for clustering using NTSYS-pc 2.11a. Roti control and Adira 4 control genotypes that were in vitro propagated without PGR addition showed different morphological characters with Roti variant and FEC-25 genotypes that were in vitro propagated with the addition of PGR. Morphological and molecular characters of 13 genotypes showed high diversity. Clustering analysis based on morphological characters classified the in vitro propagated and control plants into four groups at 45.6% similarity. Clustering analysis based on molecular characters classified the plants into three groups at 66.0% similarity.Keywords: cassava; diversity; in vitro; ISSR; morphologyABSTRAKPerbanyakan tanaman ubi kayu (Manihot esculenta Crantz) secara in vitro menggunakan zat pengatur tumbuh (ZPT) diyakini dapat menginduksi variasi morfologi. Tujuan dari penelitian ini adalah untuk menganalisis keragaman morfologi dan molekuler dari 13 genotipe ubi kayu hasil perbanyakan in vitro dan perbanyakan dengan stek batang berdasarkan 11 karakter vegetatif dan 7 penanda ISSR. Karakter morfologi dan molekuler diskor untuk analisis kelompok menggunakan program NTSYS-pc 2.11a. Genotipe Roti kontrol dan Adira 4 kontrol yang merupakan hasil perbanyakan in vitro tanpa penambahan ZPT menunjukkan perbedaan variasi morfologi dengan genotipe Roti varian dan FEC-25 yang merupakan tanaman hasil perbanyakan dengan penambahan ZPT. Hasil analisis pada 13 genotipe menunjukkan adanya keragaman yang tinggi. Hasil analisis kelompok berdasarkan penanda morfologi memisahkan antara genotipe hasil perbanyakan secara in vitro yang ditambah ZPT dengan tanaman kontrolnya ke dalam 4 kelompok dengan nilai koefisen similaritas 45,6%. Hasil analisis kelompok berdasarkan penanda molekuler memisahkan antara genotipe hasil perbanyakan secara in vitro yang ditambah ZPT dengan tanaman kontrolnya ke dalam 3 kelompok dengan nilai koefisen similaritas 66,0%.
IDENTIFIKASI DAN KARAKTERISASI BAKTERI AMILOLITIK PADA UMBI Colocasia esculenta L. SECARA MORFOLOGI, BIOKIMIA, DAN MOLEKULER Destik Wulandari; Desi Purwaningsih
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (656.658 KB) | DOI: 10.29122/jbbi.v6i2.3084

Abstract

Morphological, Biochemical, and Molecular Identification and Characterization of Amylolytic Bacteria in Tubers of Colocasia esculentaTaro tuber (Colocasia esculenta L.) has a high starch content of 77.9% so that it can be used as a substrate from which to isolate amylolytic bacteria. The purpose of this study was to isolate amylolytic bacteria from taro tubers, and subsequently to identify as well as to characterize morphologically, biochemically and molecularly using the 16S rRNA technique. Isolation of amylolytic bacteria was carried out by growing bacterial colonies on starch agar media and then selecting those colonies that had clear zones. Bacteria that produced clear zones were then characterized and identified through Gram staining, spore staining, biochemical test, and 16S rRNA molecular test. Results showed that there were seven positive isolates of amylolytic bacteria namely ECE-1, ECE-2, ECE-3, ECE-4, ECE-5, ECE-6, and ECE-7 isolates. Five isolates were identified using 16S rRNA technique. Identification results showed that the seven isolates obtained were putatively identified as Pseudomonas knackmussii, Bacillus siamensis, Bacillus siamensis, Bacillus subtilis, and Bacillus altitudinis.Keywords: 16S rRNA analysis; amylase enzyme; amylolytic bacteria; amylum; taro tuberABSTRAKUmbi talas (Colocasia esculenta L.) mempunyai kandungan pati tinggi yakni sebesar 77,9% sehingga dapat digunakan sebagai bahan untuk mengisolasi bakteri amilolitik. Tujuan penelitian ini adalah mengisolasi bakteri amilolitik dari umbi talas, dan kemudian mengidentifikasi serta mengkarakterisasi secara morfologi, biokimia dan molekuler menggunakan teknik 16S rRNA. Isolasi bakteri amilolitik dilakukan dengan cara menumbuhkan koloni bakteri pada media starch agar dan selanjutnya memilih koloni yang mempunyai zona bening. Bakteri yang menghasilkan zona bening kemudian dikarakterisasi dan diidentifikasi menggunakan metode pewarnaan Gram, pewarnaan spora, uji biokimia, dan uji molekuler 16S rRNA. Hasil menunjukkan terdapat tujuh isolat positif bakteri amilolitik yakni isolat ECE-1, ECE-2, ECE-3, ECE-4, ECE-5, ECE-6, dan ECE-7. Lima isolat diidentifikasi dengan teknik 16S rRNA. Hasil menunjukkan bahwa ketujuh isolat tersebut masing-masing secara berurutan diduga teridentifikasi sebagai Pseudomonas knackmussii, Bacillus siamensis, Bacillus siamensis, Bacillus subtilis, dan Bacillus altitudinis.
IDENTIFIKASI BAKTERI PATOGEN PENYEBAB PENYAKIT PURPLE SYNDROME PADA KARANG FUNGIA DI PULAU HARI SULAWESI TENGGARA Ratna Diyah Palupi; Baru Sadarun; Paiga Hanurin Sawonua
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1443.583 KB) | DOI: 10.29122/jbbi.v6i2.3116

Abstract

IDENTIFIKASI BAKTERI PATOGEN PENYEBAB PENYAKIT PURPLE SYNDROME PADA KARANG FUNGIA DI PULAU HARI SULAWESI TENGGARA Nowadays coral disease is one of the causes of damage to coral reefs in Indonesia. Causative agents were found for some types of coral disease. This study aims to identify the type of pathogenic bacteria that cause purple syndrome which attacks Fungia corals. The study was conducted using descriptive exploratory methods. Corals infected with purple syndrome were collected on Pulau Hari, Southeast Sulawesi, through scuba diving. Then, microbiological analysis was carried out which included isolation using the scatter method, purification using a scratch method, a challenge test (antagonistic), a Koch Postulate test, and DNA analysis of putative bacterial isolates. Results showed that 5 bacterial isolates lived in symbiosis with the corals infected with purple syndrome (PSMH1, PSMH2, PSMH3, PSMH4, and PSMH5). Based on the Koch postulate test, 2 bacterial isolates which were pathogenic were obtained, namely PSHM2 and PSHM4 isolates. These bacteria infected the test corals with the characteristics of coral skeleton damage and coral bleaching (dead). Based on biomolecular testing, the two isolates were members of Enterobacter cloacae with a 99% similarity level.Keywords: Coral disease; Enterobacter cloacae; Fungia coral; Hari island; Purple syndromeABSTRAKSaat ini penyakit karang menjadi salah satu penyebab kerusakan terumbu karang di Indonesia. Penyebab pembawa untuk beberapa jenis penyakit karang sudah ditemukan. Penelitian ini bertujuan untuk mengidentifikasi jenis bakteri patogen penyebab penyakit purple syndrome yang menyerang karang Fungia. Penelitian dilakukan menggunakan metode deskriptif eksploratif. Sampel karang yang terinfeksi purple syndrome diambil di Pulau Hari, Sulawesi Tenggara, melalui scuba diving. Selanjutnya, analisis mikrobiologi dilakukan yang meliputi isolasi menggunakan metode sebar, purifikasi menggunakan metode gores, uji tantang (antagonistik), uji Postulat Koch, dan analisa DNA isolat bakteri yang diduga bersifat patogen. Hasil penelitian menemukan 5 isolat bakteri yang bersimbiosis dengan karang yang terinfeksi penyakit purple syndrome (PSMH1, PSMH2, PSMH3, PSMH4, dan PSMH5). Berdasarkan uji postulat Koch, 2 isolat bakteri yang bersifat patogen didapatkan, yaitu isolat PSHM2 dan PSHM4. Bakteri tersebut menginfeksi karang uji dengan ciri kerusakan skeleton karang dan pemutihan karang (mati). Berdasarkan uji biomolekuler kedua isolat tersebut merupakan anggota Enterobacter cloacae dengan tingkat kemiripan 99%.

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