Garuda - Garba Rujukan Digital

EKSTRAKSI DAN IDENTIFIKASI METABOLIT SEKUNDER DARI ISOLAT AL6 SERTA POTENSINYA SEBAGAI ANTIBAKTERI TERHADAP Escherichia coli Syarifuddin, Alfian; Kamal, Sodiq; Yuliastuti, Fitriana; Pradani, Missya Putri Kurnia; Septianingrum, Ni Made Ayu Nila
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Extraction and Identification of Secondary Metabolites from AL6 IsolatesÃ‚Â and Its Potential as Antibacterial against Escherichia coliABSTRACTSecondary metabolites in the form of antibiotics can be produced by rhizospheric bacteria. AL6 bacterial isolate, which is one of the bacterial isolates from the rhosphere of Saccarum officinarum L., is known to produce antibiotic compounds. This study aims to determine the activity of antibiotics from AL6 ethyl acetate extracts produced by AL6 bacterial isolates, to analyze the minimum inhibitory concentration (MIC) and the similarity of the active substances using GCMS. The ethyl acetate extract obtained was tested for MIC at 1.25%, 2.5%, 5.0%, 10.0%, 20%, and 40% concentrations. Detection of potential antibiotic spots was carried out using bioautographic thin layer chromatography (TLC). Compounds responsible for antibiotic activity were analyzed using GCMS. Minimum inhibitory levels obtained reached 2.5%. The active spots responsible for antibiotic activity against Escherichia coli at Rf 0.94. Components detected using GCMS and suspected to be antibiotics include chloroform; ethane, 1,1-dimethoxy-(CAS) dimethyl acetal; dan 1,3-dioxolane, 2-methoxymethyl-2,4,5-trimethyl.Keywords: AL6 bacterial isolate; antibiotic; Escherichia coli; GCMS; MICABSTRAKMetabolit sekunder berupa antibiotik dapat diproduksi oleh bakteri rizosfer. Isolat bakteri AL6, salah satu isolat bakteri dari rizosfer Saccarum officinarum L., diketahui dapat menghasilkan senyawa antibiotik. Penelitian ini bertujuan mengetahui aktivitas antibiotik dari ekstrak etil asetat antibiotik AL6 yang dihasilkan isolat bakteri AL6, menganalisis kadar hambat minimum (KHM), serta kemiripan zat aktif menggunakan GCMS. Ekstrak etil asetat yang diperoleh diuji KHM-nya pada konsentrasi 1,25%, 2,5%, 5,0%, 10,0%, 20%, dan 40%. Deteksi bercak yang berpotensi sebagai antibiotik dilakukan menggunakan kromatografi lapis tipis (KLT) bioautografi. Senyawa yang berperan dalam aktivitas antibiotik dianalisis menggunakan GCMS. Kadar hambat minimal yang diperoleh mencapai 2,5%. Hasil uji KLT bioautografi memperlihatkan bercak aktif sebagai antibiotik terhadap Escherichia coli pada Rf 0,94. Komponen senyawa yang terdeteksi menggunakan GCMS dan diduga sebagai antibiotik antara lain chloroform; ethane, 1,1-dimethoxy-(CAS) dimethyl acetal; dan 1,3-dioxolane, 2-methoxymethyl-2,4,5-trimethyl.

POTENSI CEMARAN BAKTERI Escherichia coli PADA LIMBAH CAIR RUMAH POTONG AYAM (RPA) TERHADAP LINGKUNGAN DI KOTA MAGELANG Syarifuddin, Alfian; Yuliastuti, Fitriana; Pradani, Missya Putri Kurnia
Jurnal Kesehatan Vol 13, No 1 (2020): Jurnal Kesehatan
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/jk.v13i1.11101

Kualitas air secara biologis ditentukan oleh banyak parameter, antara lain parameter mikroba pencemar/ patogen. Bakteri Escherichia coli merupakan salah satu bakteri yang menjadi salah satu parameter kualitas suatu air. Jumlah kasus diare di Kota Magelang tahun 2014 mengalami kenaikan dibanding kasus diare tahun 2013, yaitu sebanyak 3.577 kasus dengan persentase sebesar 139,59% yang disebabkan oleh bakteri. Penelitian ini dilakukan untuk mengetahui ada tidaknya cemaran bakteri Escherichia coli dan mengetahui nilai Angka Lempeng Total (ALT) cemaran bakteri Escherichia coli pada air bilasan pada Rumah Potong Ayam (RPA) yang dapat berpotensi sebagai pencemar di lingkungan Kota Magelang. Metode yang digunakan dalam penelitian ini adalah cawar sebar dengan menggunakan media untuk pengujian adalah media TBX agar. Hasil penelitian menunjukkan adanya cemaran bakteri Escherichia coli yang melebihi 1x101CFU/ml pada air bilasan ayam pada beberapa RPA di Kota Magelang, yaitu 61,1% sampel uji atau sekitar 11 tempat sampling dari total sebanyak 18 tempat pemotongan ayam. Hasil tersebut menunjukkan bahwa air hasil bilasan tersebut dapat berpotensi sebagai salah satu sumber cemaran bakteri Escherichia coli pada daging ayam dan air tanah lingkungan sekitar pembuangan limbah air bilasan. Hanya terdapat 38,9% dari 18 RPA masih memenuhi ambang batas cemaran bakteri Escherichia coli. Oleh karena itu, perlu adanya langkah-langkah strategis berikutnya, yaitu pengolahan air limbah RPA dengan proses biofilter, serta dapat disosialisasikan terkait cara mengolah daging yang benar.

QUALITATIVE AND QUANTITATIVE ANALYSIS OF MEDICINAL CHEMICALS OF “ JAMU REMATIK DAN PEGAL LINU “ IN MAGELANG REGION Haresmita, Perdana Priya; Wardani, Arief Kusuma; Pradani, Missya Putri Kurnia; Permatasari, Lina
Jurnal Ilmu Kesehatan Vol 11 No 2 (2023): Jurnal Ilmu Kesehatan
Publisher : Universitas Muhammadiyah Kalimantan Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30650/jik.v11i2.3829

Jamu for rheumatism and "pegal linu" are two of the most commonly used traditional medicine products in the community, so it is a type of traditional medicine preparation that is prone to the addition of medicinal chemicals. Any type of medicinal chemical should not be added to herbal preparations. The purpose of this study was to determine is there any medicinal chemicals in traditional medicine samples for "pegal linu" and rheumatism in the Magelang area. The total number of samples used is nine. Thin layer chromatography was used to analyze medicinal chemicals in traditional medicine, and preliminary organoleptic test was performed. The next step is a quantitative analysis using the UV-Visible spectrophotometric method. Prednisone, mefenamic acid, and ibuprofen were used as standards. The stationary phase was silica gel 60 F254, and the mobile phase was ethyl acetate: chloroform (4:1) for the prednisone standard, chloroform:methanol (9:1) for the mefenamic acid standard, and chloroform:ethanol (8:1) for the ibuprofen standard. Thin layer chromatography analysis showed that three of the nine traditional medicine samples tested positive for prednisone, but none contained mefenamic acid or ibuprofen. Herbs A, D, and E contained prednisone in their samples. Prednisone concentrations in the three samples were 0,252%; 0.754%, and 2,005%, respectively.

FORMULASI DAN UJI AKTIVITAS ANTIOKSIDAN SEDIAAN MASKER GEL PEEL OFF EKSTRAK ETANOL DAUN PEGAGAN (Centella asiatica (L.) Urban) Putri, Meilisa; Capritasari, Rafiastiana; Wijayatri, Ratna; Ningrum, Yessika Ardian; Pradani, Missya Putri Kurnia
BORNEO JOURNAL OF PHARMASCIENTECH Vol 8 No 2 (2024): Borneo Journal of Pharmascientech
Publisher : Universitas Borneo Lestari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51817/bjp.v8i2.612

Free radicals damage cellular structures and accelerate the degradation of important elements in the skin, thereby accelerating premature aging. Antioxidants in skin care products can shield cells from harm brought on by oxidative stress. Pegagan leaves (Centella asiatica (L.) Urban) are a natural source of antioxidants. Pegagan leaf ethanolic extract has antioxidant properties and can perhaps be applied in cosmetic products like peel-off mask gel. The objective of this research is to measure the antioxidant behavior of a mask that peels off gel composed of ethanolic extract of Pegagan leaves (Centella asiatica (L.) Urban). This research applies a one-shot case study laboratory experimental method. Pegagan ethanolic extract was utilized in concentrations of F1 (0.5% ) F2 (1%) and F3 (1.5%). The level of antioxidant activity was measured. for three formulae using the DPPH (2,2-diphenyl-1-picrylhydrazyl) technique. Organoleptic, pH measurement, viscosity, homogeneity, spreadability, and drying time are all considered in the physical evaluation of the preparation. Based on evaluation outcomes of the preparations, three formulas met the test requirements with organoleptic test parameters, a drying time of 15-30 minutes, and a pH value between 4.5 and 6.5. For the spreadability test, only F2 meets the test requirements with 5-7 cm, while F1 and F3 do not follow the criteria because the A preparation's spreadability is influenced by viscosity. The thicker the mask gel preparation, the smaller the resulting spreading area. The result examination for antioxidant activity on the ethanolic extract Pegagan leaf showed an IC50 value for F1 of 28.68 ppm, F2 of 38.51 ppm, and F3 of 48.68 ppm. Study shows making the peel-off mask prepared gel Pegagan ethanol extract meets the criteria for a strong antioxidant. The results of this research indicate the quantity of antioxidant function in addition to the outcomes of the physical assessment have an impact on the concentration of pegagan leaf extract in the gel mask that peels off.

QUALITATIVE AND QUANTITATIVE ANALYSIS OF MEDICINAL CHEMICALS OF “ JAMU REMATIK DAN PEGAL LINU “ IN MAGELANG REGION Haresmita, Perdana Priya; Wardani, Arief Kusuma; Pradani, Missya Putri Kurnia; Permatasari, Lina
Jurnal Ilmu Kesehatan Vol 11 No 2 (2023): Jurnal Ilmu Kesehatan
Publisher : Universitas Muhammadiyah Kalimantan Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30650/jik.v11i2.3829

Jamu for rheumatism and "pegal linu" are two of the most commonly used traditional medicine products in the community, so it is a type of traditional medicine preparation that is prone to the addition of medicinal chemicals. Any type of medicinal chemical should not be added to herbal preparations. The purpose of this study was to determine is there any medicinal chemicals in traditional medicine samples for "pegal linu" and rheumatism in the Magelang area. The total number of samples used is nine. Thin layer chromatography was used to analyze medicinal chemicals in traditional medicine, and preliminary organoleptic test was performed. The next step is a quantitative analysis using the UV-Visible spectrophotometric method. Prednisone, mefenamic acid, and ibuprofen were used as standards. The stationary phase was silica gel 60 F254, and the mobile phase was ethyl acetate: chloroform (4:1) for the prednisone standard, chloroform:methanol (9:1) for the mefenamic acid standard, and chloroform:ethanol (8:1) for the ibuprofen standard. Thin layer chromatography analysis showed that three of the nine traditional medicine samples tested positive for prednisone, but none contained mefenamic acid or ibuprofen. Herbs A, D, and E contained prednisone in their samples. Prednisone concentrations in the three samples were 0,252%; 0.754%, and 2,005%, respectively.

Title

Found 5 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 5 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 5 Documents
Search