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Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
JBBI, Indonesian Journal of Biotechnology & Bioscience, is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, as well as life sciences in general. Initiated at the then Biotech Centre, the journal is published by the Laboratory for Biotechnology, the Agency for the Assessment and Application of Technology, BPPT.
Arjuna Subject : -
Articles 542 Documents
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PENGARUH SEREAL BERBAHAN SAGU DAN Moringa oleifera TERHADAP KADAR GLUKOSA DARAH TIKUS YANG DIINDUKSI ALOKSAN Herada Putri, Melin Novidinisa; Fauziyah, A'immatul; Maryusman, Taufik
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.063 KB) | DOI: 10.29122/jbbi.v6i2.3669

Abstract

The Effects of Cereal Made From Sagu and Moringa oleifera on the Blood Glucose Level of Alloxan-Induced RatsDiabetes Mellitus (DM) type 2 could increase oxidative stress and blood glucose level. Resistant starch compounds in instant cereal Cersa Mori have antidiabetic properties. This research aimed to analyze the effect of Cersa Mori on fasting blood glucose (FBG) levels of diabetic white rats induced by alloxan. This is a true experimental study with a randomized pre-post control group design using 27 male Wistar strain rats divided into 3 groups randomly, i.e (KN) feed and distilled water, (KP) glibenclamide 0.126mg/200gBB/day, (P) Cersa Mori 5g/200gBB/day. KP and P groups were given alloxan 125 mg/KgBB subcutaneously and the intervention was carried out for 30 days. FBG level was measured using the GOD-PAP method. The results of Paired T-Test showed the effect of Cersa Mori on lowering FBG levels in hyperglycemic rats (P = 0,006). One-Way ANOVA test showed that Cersa Mori reduced FBG level, which was equivalent to those given glibenclamide (P = 0,366). It can be concluded that giving Cersa Mori 5g/200gBB/day for 30 days had a significant effect on lowering FBG level. Keywords: alloxan; Cersa Mori; diabetic rats; fasting blood glucose level; resistant strachABSTRAKDiabetes Mellitus (DM)tipe 2 dapat memicu stres oksidatif dan meningkatkan kadar glukosa darah puasa (GDP). Senyawa pati resisten dalam sereal siap saji Cersa Mori memiliki sifat antidiabetik. Penelitian ini bertujuan menganalisis pengaruh pemberian Cersa Mori terhadap kadar glukosa darah tikus putih diabetes yang diinduksi aloksan. Penelitian true-experimental ini menggunakan randomized pre-post control group design. Sampel sebanyak 27 ekor tikus jantan galur Wistar dibagi menjadi 3 kelompok secara acak yaitu; (KN) pakan dan akuades, (KP) glibenklamid 0,126 mg/200gBB/hari, (P) Cersa Mori 5g/200gBB/hari. KP sampai P diberikan aloksan 125 mg/KgBB secara subkutan dan intervensi dilakukan selama 30 hari. Pengukuran GDP menggunakan metode GOD-PAP. Hasil Uji-T menunjukkan pengaruh Cersa Mori dalam menurunkan GDP tikus hiperglikemia (P = 0,006). Uji ANOVA satu arah menunjukkan penurunan GDP pada kelompok Cersa Mori (P) setara dengan tikus yang diberi glibenklamid (P = 0,366). Dengan demikian dapat disimpulkan bahwa pemberian Cersa Mori dosis 5g/200gBB/hari selama 30 hari berpengaruh terhadap penurunan GDP secara signifikan.
Back Cover JBBI Vol 3, No 2, December 2016 Sriherwanto, Catur
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 3 No. 2 (2016): December 2016
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (261.832 KB) | DOI: 10.29122/jbbi.v3i2.1057

Abstract

EFEKTIVITAS MERKURI KLORIDA (HgCl2) PADA STERILISASI TUNAS SAMPING JATI (Tectona grandis) IN VITRO Fauzan, Yusuf Sigit Ahmad; Supriyanto, .; Tajuddin, Teuku
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 4 No. 2 (2017): December 2017
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (966.189 KB) | DOI: 10.29122/jbbi.v4i2.2540

Abstract

Effectiveness of Mercury Chloride (HgCl2) in Sterilization of Teak (Tectona grandis L.f.) In VitroThe main obstacle in obtaining sterile materials in in vitro cultures derived from meristems is high level of surface contamination caused by fungi and bacteria, which often results in explant death. The objective of this study was to obtain an appropriate mercury chloride (HgCl2) concentration for the sterilization of Tectona grandis nodes in in vitro culture. One cm long-sized nodes with 0.2 mm diameter were immersed in HgCl2at concentrations of 0, 100, 200 and 300 mg/L for 3 minutes. The results showed that the higher concentration of HgCl2was able to suppress the growth of fungi and bacteria and increased the percentage of aseptic explants. The best HgCl2concentration was 300 mg/L since it suppressed the growth of fungi and bacteria up to 100% and 75%, respectively, and produced the highest aseptic explants of 85% at 9 days after treatment. The small sized explants supported the sterilization process and reduced browning levels.Keywords: Browning, HgCl2, in vitro, sterilization, T. grandisABSTRAKKendala utama dalam mendapatkan material steril pada kultur in vitro yang berasal dari meristem adalah tingginya tingkat kontaminasi permukaan yang disebabkan oleh jamur dan bakteri, dan sering menyebabkan kematian eksplan. Tujuan penelitian ini adalah untuk memperoleh konsentrasi merkuri klorida (HgCl2) yang tepat untuk sterilisasi eksplan tunas samping tanaman jati (Tectona grandis) pada kultur in vitro. Tunas samping berukuran 1 cm dan diameter 0,2 mm direndam dalam HgCl2 pada konsentrasi 0, 100, 200 dan 300 mg/L selama 3 menit. Hasil penelitian menunjukkan bahwa penambahan konsentrasi HgCl2 yang semakin tinggi mampu menekan pertumbuhan jamur dan bakteri pada eksplan serta meningkatkan persentase eksplan aseptik. HgCl2 dengan konsentrasi 300 mg/L merupakan konsentrasi terbaik karena dapat menekan pertumbuhan jamur hingga 100% dan bakteri mencapai 75%, serta menghasilkan tingkat eksplan aseptik dan hidup tertinggi yaitu sebesar 85% pada 9 hari setelah perlakuan. Ukuran eksplan yang kecil mendukung proses sterilisasi dan mengurangi tingkat browning. Kata kunci: HgCl2,in vitro, pencoklatan jaringan, sterilisasi, T. grandis, Received: 02 November 2017                 Accepted: 14 December 2017                Published: 29 December 2017
KEMAMPUAN EKSTRAK SENYAWA AKTIF BAKTERI ENDOFIT DALAM MENGHAMBAT PERTUMBUHAN Fusarium oxysporum f.sp. PADA KELAPA SAWIT Candrawati, Emilia; Rupaedah, Bedah; Sumpono, Sumpono; Sundaryono, Agus
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 2 (2018): December 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (921.358 KB) | DOI: 10.29122/jbbi.v5i2.2769

Abstract

Ability of Active Compound Extract of Endophytic Bacteria to Inhibit the Growth of Fusarium oxysporum f.sp. in Oil Palm ABSTRACTWilt vessels disease in oil palm plants is caused by Fusarium oxysporum f.sp. This disease is very harmful because of its ability to kill the infected oil palm plant in less than a year. Endophytic bacteria are likely to be biological controllers for the disease because of their ability to produce bioactive antifungal compounds. Isolation of endophytic bacteria from oil palm plant and activity test of their active compounds against F. oxysporum f.sp. in vitro had been done. Antagonistic test of endophytic bacterial isolates against F. oxysporum f.sp. was carried out using a double culture method. The potential endophytic bacterial isolates were extracted using ethyl acetate solvent for their active compounds, which were then tested for its activity in inhibiting the growth of F. oxysporum f.sp. The results showed that the active compound extract of B11 endophytic bacteria with the incubation time of 24 and 54 hours gave the growth inhibition of F. oxysporum f.sp. at the level of 29.23% and 43.85%, respectively.Keywords: antagonistic test, bioactive compound, endophytic bacteria, F. oxysporum f.sp., oil palm ABSTRAKPenyakit layu pembuluh pada tanaman kelapa sawit disebabkan oleh Fusarium oxysporum f.sp. Penyakit ini menjadi penyebab kematian tanaman kelapa sawit yang telah terinfeksi dalam waktu kurang dari setahun. Bakteri endofit asal tanaman kelapa sawit dimungkinkan menjadi pengendali hayati bagi penyakit ini karena kemampuan bakteri tersebut memproduksi senyawa bioaktif yang bersifat antifungi. Isolasi bakteri endofit dari tanaman kelapa sawit dan uji aktivitas senyawa aktifnya terhadap F. oxysporum f.sp. secara in vitro telah dilakukan. Uji antagonis isolat bakteri endofit terhadap jamur patogen F. oxysporum f.sp. menggunakan metode kultur ganda. Isolat bakteri endofit potensial diekstrak senyawa aktifnya dengan menggunakan pelarut etil asetat, kemudian senyawa aktif ini diuji aktivitasnya dalam menghambat pertumbuhan jamur patogen F. oxysporum f.sp. Hasil penelitian menunjukkan bahwa ekstrak senyawa aktif bakteri endofit B11 dengan waktu inkubasi 24 dan 54 jam memberikan daya hambat terhadap F. oxysporum f.sp. sebesar masing-masing 29,23% dan 43,85%.Kata Kunci: bakteri endofit, F. oxysporum f.sp., kelapa sawit, senyawa aktif, uji antagonis 
Back Cover JBBI Vol 6, No 1, June 2019 Sriherwanto, Catur
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (83.157 KB) | DOI: 10.29122/jbbi.v6i1.3618

Abstract

TINJAUAN LOVASTATIN DAN APLIKASINYA Hardianto, Dudi
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 1 No. 1 (2014): December 2014
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (909.8 KB) | DOI: 10.29122/jbbi.v1i1.550

Abstract

Lovastatin is a drug belonging to statins group that is used to decrease the cholesterol levels in blood. The action mechanism of lovastatin is inhibition of the activity of HMG-CoA reductase enzyme, hence reducing cholesterol production in the liver. Some filamentous fungi produce lovastatin, and Aspergillus terreus is known as the highest lovastatin-producing filamentous fungi, therefore it is generally used for production of lovastatin. Commercial production of lovastatin is based on submerged fermentation. But nowadays solid-state fermentation is becoming an alternative for production of lovastatin. Lovastatin is mainly used for antihypercholeterolemia. Other potential uses of lovastatin include therapy of Alzheimer’s disease, cancer, osteoporosis, Parkinson’s disease, multiple sclerosis, and rheumatoid arthritis.Keywords: Statin, lovastatin, Aspergillus terreus, fermentation, antihypercholeterolemia ABSTRAK Lovastatin merupakan obat golongan statin yang digunakan untuk menurunkan kadar kolesterol dalam darah. Mekanisme kerja lovastatin adalah menghambat enzim HMG-CoA reduktase sehingga produksi kolesterol di dalam hati berkurang. Beberapa kapang berfilamen memproduksi lovastatin dan Aspergillus terreus merupakan kapang penghasil lovastatin tertinggi sehingga digunakan dalam produksi lovastatin. Produksi lovastatin secara komersial menggunakan fermentasi cair tetapi sekarang ini fermentasi padat menjadi alternatif lain untuk memproduksi lovastatin. Lovastatin digunakan terutama untuk antihiperkolesterolemia. Lovastatin juga potensial digunakan untuk pengobatan penyakit alzheimer, kanker, osteoporosis, parkinson, multiple sclerosis, dan rheumatoid arthritis.Kata kunci: Statin, lovastatin, Aspergillus terreus, fermentasi, antihypercholeterolemia
PENGARUH BEBERAPA JENIS SITOKININ PADA MULTIPLIKASI TUNAS ANGGREK Vanda douglas SECARA IN VITRO Karyanti, Karyanti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 4 No. 1 (2017): June 2017
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (792.84 KB) | DOI: 10.29122/jbbi.v4i1.2200

Abstract

The Effect of Several Types of Cytokinin on Shoot Multiplication of Vanda douglas Orchid In VitroThe study was aimed to determine the response of Vanda douglas orchid on shoot-multiplication media to different cytokinin concentrations in vitro. A completely randomized design experiment was employed with one factor cytokinin, in which the cytokinins used were TDZ (thidiazuron), BAP (6-Benzylaminopurine) and kinetin at the concentrations of 0, 0.5, 1, and 1.5 mg/L. The results showed that kinetin 0.5 mg/L was the best concentration for shoot formation, occuring on average at 14.88 days after planting; while TDZ 0.5 mg/L was the best concentration for increasing the height of the plant, being on average 0.53 cm. TDZ at 0.5 mg/L concentration also had positive effect on shoot and leaf formation, which resulted in the highest number of shoots and leaves. The average number of shoots was 8.00 buds, and the average number of leaves was 12.25 sheets. Keywords: Vanda douglas, thidiazuron, BAP, kinetin, shoots multiplication ABSTRAKPenelitian ini bertujuan untuk mengetahui respon anggrek Vanda douglas terhadap media perbanyakan tunas pada beberapa konsentrasi jenis sitokinin secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan satu faktor, yaitu sitokinin. Sitokinin yang digunakan adalah TDZ (thidiazuron), BAP (6-Benzylaminopurine) dan kinetin, dengan konsentrasi 0, 0,5, 1, dan 1,5 mg/L. Hasil penelitian menunjukkan bahwa pemberian kinetin 0,5 mg/L menunjukkan hasil terbaik pada peubah waktu pembentukan tunas, dengan rata-rata 14,88 hari setelah tanam. Sedangkan konsentrasi TDZ 0,5 mg/L merupakan jenis sitokinin dan konsentrasi terbaik terhadap pertambahan tinggi tanaman, dengan rata-rata 0,53 cm. TDZ dengan konsentrasi 0,5 mg/L juga berpengaruh positif terhadap pertumbuhan tunas dan daun, dengan menghasilkan jumlah tunas tertinggi dan jumlah daun terbanyak. Rata-rata jumlah tunas adalah 8,00 tunas, dan rata-rata jumlah daun adalah 12,25 helai.Kata Kunci: Vanda douglas, thidiazuron, BAP, kinetin, perbanyakan tunasReceived: 19 June 2017        Accepted: 02 July 2017        Published: 12 July 2017
DAMPAK TEKNIK PENGIRISAN DAN PENCETAKAN TERHADAP DAYA APUNG PAKAN IKAN YANG DIFERMENTASI MENGGUNAKAN Rhizopus sp. Suliswati, Lulu; Sriherwanto, Catur; Suja'i, Imam
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 2 (2018): December 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v5i2.3096

Abstract

Impact of Slicing and Moulding Techniques on the Floatability of the Fish Feed Fermented by Rhizopus sp.ABSTRACTThe use of Rhizopus sp. mycelium as biocoating, biostabilizing, and biofloating agent in the production of floating fish feed through solid fermentation had already been studied as a much simpler alternative to mechanical extrusion. The fermented fish feed, however, had poor floatability in aerated water, probably due to structural damage during the size reduction process of the feed. Thus, this study used alternative size-reducing methods, namely slicing and moulding, to improve the floatability of the fermented feed. Other physical characteristics were also measured and compared to those of commercial sinking and floating fish feeds. Results showed that both the moulded and the sliced fermented-feeds had lower density as well as higher water stability, absorption capacity, floatability, and durability compared to those of the commercial sinking feed used as the fermentation substrate. The hydrophobicity of all the feeds tested were similar, however. The floatability of the fermented feeds obtained in this study was much higher than those of the previous studies. Keywords: floatability, floating feed, sinking feed, water absorption, water stabilityABSTRAKPenggunaan miselium Rhizopus sp. sebagai pelapis permukaan, penstabil, dan pengapung hayati dalam pembuatan pakan ikan apung melalui fermentasi padat telah diteliti sebagai alternatif yang jauh lebih sederhana dibandingkan dengan metode ekstrusi mesin. Namun, pakan ikan fermentasi ini memiliki daya apung yang buruk dalam air bergelembung udara, yang mungkin disebabkan kerusakan struktural selama proses pengecilan ukuran pakan. Karenanya, penelitian ini menggunakan metode lain untuk mengecilkan ukuran, yakni pencetakan dan pengirisan, dalam rangka meningkatkan daya apung pakan yang difermentasi. Karakteristik fisik lainnya juga diukur dan dibandingkan dengan pakan ikan tenggelam dan terapung komersial. Hasil menunjukkan bahwa proses fermentasi serta metode pengecilan dimensi yang digunakan menghasilkan pakan yang memiliki massa jenis lebih rendah, serta stabilitas air, daya serap air, daya apung, serta ketahanan benturan lebih tinggi dibandingkan dengan pakan tenggelam komersial yang digunakan sebagai substrat fermentasi. Namun, nilai hidrofobisitas semua pakan yang diuji adalah sama. Daya apung pakan fermentasi dalam penelitian ini jauh lebih tinggi daripada penelitian sebelumnya.Kata Kunci: daya apung, daya serap air, stabilitas dalam air, pakan apung, pakan tenggelam
MIKROPROPAGASI TANAMAN TALAS BENENG (Xanthosoma undipes K. Koch) DENGAN PERLAKUAN BENZIL AMINOPURIN, TIAMIN, DAN ADENIN Sari, Laela; Wulansari, Aida; Noorrohmah, Siti; Ermayanti, Tri Muji
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v6i1.3216

Abstract

Micropropagation of Beneng Taro (Xanthosoma undipes K. Koch) with Benzyl Amino Purine, Thiamine, and Adenine TreatmentABSTRACTConventional production of Beneng taro seeds (Xanthosoma undipes K. Koch) is constrained by the limited number of tubers, thus an alternative solution is needed such as in vitro propagation. This study was aimed to obtain a micropropagation technique of Beneng taro on MS media with BAP, thiamine, and adenine treatment, and to determine its growth at the acclimatization stage. This research consisted of shoot multiplication and acclimatization. Shoot propagation was carried out on MS media with 8 treatments, namely ½MS and MS without addition of growth promoting substance, and MS with 1, 2 and 3 mg×L-1 BAP, with or without addition of 1 mg×L-1 thiamine and 2 mg×L-1 adenine. Each treatment was replicated four times, each consisting of four shoots. Growth observation was made from 1st to 5th week on petiole length, and number of shoots, leaves and roots. Acclimatization was carried out on soil media, compost, and husks in a ratio of 1: 1: 1. The results showed that the best media for shoot multiplication was MS + 1 mg×L-1 BAP + 1 mg×L-1 thiamine + 2 mg×L-1 adenine with an average of 3.5 shoots, while the best medium for the petiole length was ½MS with an average value of 6.97 cm. The results of acclimatization showed that 100% planlets survived, and plantlets grown on MS media + 3 mg×L-1 BAP had the highest number of shoots with an average of 4.2.Keywords: adenine, Beneng taro, benzil amino purine (BAP), micropropagation, thiamineABSTRAKPenyediaan bibit talas Beneng (Xanthosoma undipes K. Koch) secara konvensional terkendala terbatasnya jumlah umbi, sehingga perlu solusi alternatif, diantaranya melalui perbanyakan in vitro. Tujuan penelitian ini adalah untuk mendapatkan teknik mikropropagasi talas beneng pada media MS dengan perlakuan BAP, tiamin, adenin, dan untuk mengetahui pertumbuhannya pada tahap aklimatisasi. Penelitian ini meliputi perbanyakan tunas dan aklimatisasi. Perbanyakan tunas menggunakan media MS dengan 8 perlakuan yaitu ½MS dan MS tanpa penambahan zat pengatur tumbuh (ZPT), serta MS dengan 1, 2 dan 3 mg×L-1 BAP dengan atau tanpa penambahkan 1 mg×L-1 tiamin dan 2 mg×L-1 adenin. Setiap perlakuan mempunyai empat ulangan, setiap ulangan terdiri atas empat tunas. Pertumbuhan diamati mulai minggu ke-1 hingga ke-5 terhadap panjang petiol serta jumlah anakan, daun dan akar. Aklimatisasi dilakukan pada media tanah, kompos dan sekam dengan perbandingan 1:1:1. Hasil menunjukkan bahwa media terbaik perbanyakan tunas adalah MS + 1 mg×L-1 BAP + 1 mg×L-1 tiamin + 2 mg×L-1 adenin dengan rata-rata 3,5 tunas, sedangkan media terbaik untuk panjang tangkai daun adalah ½MS dengan nilai rata-rata 6,97 cm. Hasil aklimatisasi menunjukkan bahwa 100% planlet hidup dan planlet yang ditumbuhkan pada media MS + 3 mg×L-1 BAP mempunyai jumlah anakan terbanyak dengan rata-rata 4,2.Kata Kunci: adenine, benzil amino purin (BAP), mikropropagasi, talas Beneng, tiamine
PENICILLIN PRODUCTION BY MUTANT OF Penicillium chrysogenum Hardianto, Dudi; ., Suyanto; Prabandari, Erwahyuni Endang; Windriawati, Lira; Marwanta, Edy; ., Tarwadi
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 2 No. 1 (2015): June 2015
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (785.364 KB) | DOI: 10.29122/jbbi.v2i1.530

Abstract

Penisilin adalah antibiotika yang pertama kali ditemukan dan digunakan untuk pengobatan infeksi bakteri. Sejak ditemukan penisilin sebagai antibiotika oleh Alexander Fleming pada tahun 1928, banyak usaha dilakukan untuk meningkatkan produktivitas Penicillium chrysogenum. Pemuliaan galur untuk meningkatkan produksi penisilin dapat menggunakan mutasi acak secara fisika dan kimia. Pada penelitian ini, radiasi sinar ultraviolet digunakan untuk mendapatkan mutan P. chrysogenum. Produksi penisilin ditentukan menggunakan HPLC dan produktivitas mutan dibandingkan dengan induk P. chrysogenum. Mutan M12 menghasilkan penisilin 1,23 kali lebih banyak dibandingkan dengan induk P. chrysogenum.Kata kunci: Penisilin, Penicillium chrysogenum, ultraviolet, mutan, radiasi ABSTRACTPenicillin is the first antibiotic discovered and used for treatment of bacterial infections. Since the discovery of penicillin as antibiotic by Alexander Fleming in 1928, much effort has been invested to improve productivity of Penicillium chrysogenum. Strain improvement to increase the penicillin production can be carried out by physical and chemical random mutation. In this research, ultraviolet irradiation was used to obtain P. chrysogenum mutant. Penicillin production was determined by using HPLC and productivity of P. chrysogenum mutants was compared to the wild type. Mutant M12 produced 1.23 fold higher penicillin than the wild type did.Keywords: Penicillin, Penicillium chrysogenum, ultraviolet, mutant, radiation

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