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INDONESIA
Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
JBBI, Indonesian Journal of Biotechnology & Bioscience, is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, as well as life sciences in general. Initiated at the then Biotech Centre, the journal is published by the Laboratory for Biotechnology, the Agency for the Assessment and Application of Technology, BPPT.
Arjuna Subject : -
Articles 542 Documents
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PENGARUH SINBIOTIK KEFIR TEPUNG PISANG BATU (Musa balbisiana) TERHADAP KADAR GLUKOSA DARAH TIKUS SINDROM METABOLIK Salsabila, Dhea Marliana; Maryusman, Taufik; Fatmawati, Iin
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (111.675 KB) | DOI: 10.29122/jbbi.v7i1.3730

Abstract

Metabolic syndrome begins with insulin resistance characterized by hyperglycemia. Synbiotic kefir banana (Musa balbisiana Colla) flour reduces blood glucose level. This study was conducted to analyze the effects of synbiotic kefir banana (M. balbisiana) flour on blood glucose level of metabolic syndrome rats. This study used 24 Sprague Dawley.rats which were divided into four groups, i.e. negative control was given standard diet, positive control was given high fat fructose diet (HFFD), treatment I (PI) and treatment II (PII) were given HFFD and synbiotic kefir banana (M. balbisiana) flour 1.8 mL 200 g־¹ rat BW per day (PI) and 3.6 mL 200 g־¹ rat BW per day (PII), respectively, for three weeks. The result showed a significant difference (p=0.000) in blood glucose after giving synbiotic kefir banana (M. balbisiana) flour. Synbiotic kefir banana (M. balbisiana) flour reduced blood glucose level in metabolic syndrome rats.Keywords: banana flour, blood glucose level, kefir, metabolic syndrome, synbiotic ABSTRAKSindrom metabolik diawali resistensi insulin yang ditandai hiperglikemia. Sinbiotik kefir tepung pisang batu (M. balbisiana) menurunkan kadar glukosa darah. Penelitian ini dilakukan untuk menganalisis pengaruh sinbiotik kefir tepung pisang batu (M. balbisiana) terhadap kadar glukosa darah tikus sindrom metabolik. Penelitian ini menggunakan 24 tikus Sprague Dawley yang dibagi menjadi empat kelompok, yaitu kontrol negatif yang diberi pakan standar, kontrol positif yang diberi high fat fructose diet (HFFD), dan perlakuan I (PI) serta perlakuan II (PII) yang masing-masing diberi HFFD dan sinbiotik kefir tepung pisang batu (M. balbisiana) 1,8 mL 200 g־¹ BB tikus per hari (PI) dan 3,6 mL 200 g־¹ BB tikus per hari (PII) selama tiga minggu. Hasil menunjukkan perbedaan kadar glukosa darah setelah pemberian sinbiotik kefir tepung pisang batu (M. balbisiana) secara signifikan (p=0,000). Sinbiotik kefir tepung pisang batu (M. balbisiana) menurunkan kadar glukosa darah tikus sindrom metabolik.
BIOKONVERSI SEFALOSPORIN C MENJADI ASAM 7-AMINOSEFALOSPORANAT DENGAN SEFALOSPORIN ASILASE Hardianto, Dudi; Isdiyono, Bima Wedana; Ivan, Fransiskus Xaverius
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 3 No. 2 (2016): December 2016
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (921.244 KB) | DOI: 10.29122/jbbi.v3i2.139

Abstract

Cephalosporins are the most widely used class of β-lactam antibiotic in the world and clinically active against gram positive and gram negative bacteria. Cephalosporin C (CPC) is naturally produced by fungus Cephalosporiun acremonium. CPC has moderate antibacterial activity with minimum inhibitory concentration values of 25-100 µg/mL and 12-25 µg/mL for gram-positive and for gram-negative bacteria, respectively. CPC can be converted into 7-aminocephalosporonic acid (7-ACA) as intermediate compound for cephalosporin derivatives by two-steps or one-step enzymatic method. Two-step enzymatic method uses D-amino acid oxidase (DAAO) to produce glutaryl-7-amino cephalosporanic acid (GL 7-ACA) for the first step and GL-7-ACA acylase to produce 7-ACA for the second step. One-step enzymatic method uses CPC acylase to convert CPC into 7-ACA directly. Some microorganisms produce CPC acylase, such as Pseudomonas sp., Bacillus megaterium, Aeromonas sp., dan Arthrobacler. A natural CPC acylase has low activity and genetic engineering was used to increase its activity.Keywords: Cephalosporin, cephalosporin acylase, 7-ACA, genetic engineering, mutation ABSTRAKSefalosporin merupakan antibiotik golongan β-laktam yang paling banyak digunakan di dunia dan secara klinis aktif terhadap bakteri gram positif dan gram negatif. Sefalosporin C merupakan sefalosporin alami yang dihasilkan oleh kapang Cephalosporium acremonium. Sefalosporin C mempunyai aktivitas antibakteri moderat dengan nilai konsentrasi hambat minimum 25-100 µg/mL untuk bakteri gram positif dan 12-25 µg/mL untuk bakteri gram negatif. Sefalosporin C dapat diubah menjadi asam 7-aminosefalosporanat (7-ACA) sebagai senyawa antara untuk pembuatan turunan sefalosporin dengan metode enzimatik secara dua atau satu tahap. Produksi 7-ACA secara enzimatik dapat menggunakan metode dua tahap dan satu tahap enzimatik. Metode enzimatik secara dua tahap menggunakan enzim asam D-amino oksidase (DAAO) untuk menghasilkan asam glutaril-7-aminosefalosporinat (GL-7-ACA) pada tahap pertama dan menggunakan asam glutaril-7-aminosefalosporinat asilase untuk menghasilkan 7-ACA pada tahap kedua. Metode enzimatik secara satu tahap menggunakan sefalosporin asilase untuk mengubah CPC menjadi 7-ACA secara langsung. Beberapa mikroorganisme penghasil sefalosporin asilase yaitu Pseudomonas sp., Bacillus megaterium, Aeromonas sp., dan Arthrobacter. Aktivitas CPC asilase alami sangat rendah dan rekayasa genetik digunakan untuk meningkatkan aktivitasnya.Kata kunci : Sefalosporin, sefalosporin asilase, 7-ACA, rekayasa genetik, mutasi
MULTIPLIKASI IN VITRO ANGGREK HITAM (Coelogyne pandurata Lindl.) PADA PERLAKUAN KOMBINASI NAA DAN BAP Kartiman, Roni; Sukma, Dewi; Aisyah, Syarifah Iis; Purwito, Agus
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 1 (2018): June 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1006.134 KB) | DOI: 10.29122/jbbi.v5i1.2908

Abstract

In Vitro Multiplication of  Black Orchid (Coelogyne pandurata Lindl.) Using the Combination of NAA and BAPABSTRACTBlack orchid is an indigenous plant from Kalimantan, Indonesia. It becomes endangered because of forest over-exploitation and its low natural reproduction rate. Tissue culture is considered to offer a solution to conserve and propagate this species. The aim of this research was to evaluate the effect of Naphtalene Acetic Acid (NAA) and 6-Benzile Amino Purine (BAP) on shoots multiplication of black orchid. The basic medium used was a half of Murashige & Skoog (MS) composition supplemented with 150 mLL-1 coconut water. Initial explants used were 6-month-old shoots of germinating seeds. The shoot cultures were incubated for 23 weeks. Results showed that the best combination for shoot multiplication was NAA 0.0 mgL-1 with BAP 0.2 mgL-1. Shoot grew better on medium with BAP and without NAA while roots growth was better on medium without the two plant growth regulators. The addition of BAP up to 0.3 mgL-1 increased the leaf number, which however decreased at higher BAP concentration.Keywords: BAP, black orchid, Coelogyne pandurata, multiplication, NAA ABSTRAKAnggrek hitam merupakan flora langka asli Kalimantan, Indonesia. Keberadaa anggrek ini di alam semakin langka akibat eksploitasi berlebihan dan sulitnya perbanyakan secara alami. Kultur jaringan merupakan metode untuk mengatasi kelangkaan anggrek ini. Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi NAA dan BAP terhadap multiplikasi anggrek hitam. Media dasar yang digunakan adalah ½ MS dengan penambahan air kelapa 150 mLL-1. Eksplan yang digunakan adalah tunas hasil semai biji umur 6 bulan. Kultur tunas diinkubasi selama 23 minggu. Hasil penelitian menunjukkan bahwa kombinasi terbaik untuk multiplikasi tunas adalah NAA 0 mgL-1 dengan BAP 0,2 mgL-1. Tunas tumbuh lebih baik dalam media dengan penambahan BAP tanpa NAA, sedangkan akar pada media tanpa NAA dan BAP. Penambahan BAP sampai 0.3 mgL-1 mampu meningkatkan jumlah daun, namun menurun dengan penambahan di atas konsentrasi tersebut.Kata Kunci: anggrek hitam, BAP, Coelogyne pandurata, multiplikasi, NAA
Back Cover JBBI Vol 5, No 2, December 2018 Sriherwanto, Catur
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 2 (2018): December 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (83.157 KB) | DOI: 10.29122/jbbi.v5i2.3291

Abstract

PRODUKSI BIOETANOL DARI BATANG TUA KELAPA SAWIT DENGAN AIR PANAS BERTEKANAN DAN BALL MILL ., Suyanto; Matsunaga, Kotetsu; Inoue, Hiroyuki; Sakanishi, Kinya
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 2 No. 2 (2015): December 2015
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (618.712 KB) | DOI: 10.29122/jbbi.v2i2.508

Abstract

Bioethanol from oil palm trunk is one important source of energy to reduce greenhouse gas emissions and to avoid competition between the use for food and for energy as happens in other commodities. Pre-treatment of lignocellulosic biomass using sulfuric acid has high efficiency but requires high cost to neutralize the byproducts formed. Pre-treatment using hot compressed water and ball mill can be used to break down the structure and composition of lignocellulose so as to increase the rate of enzymatic hydrolysis and increase the sugar yield. Hydrolysis using an enzyme cocktail with hot compressed water and ball mill can each produce 210 mg of glucose at a temperature of 220°C and 200 mg of glucose for 120 minutes at room temperature per gram of oil palm trunk. Both of these processes are environmentally friendly and have a high efficiency in the production of bioethanol from oil palm trunks.Keywords: bioethanol, oil palm trunk, ball mill, hot compressed water, hydrolysis  ABSTRAKBioetanol dari batang kelapa sawit adalah salah satu sumber energi yang penting untuk mengurangi emisi rumah kaca dan menghindari konflik kepentingan antara kebutuhan bahan pangan dan penggunaan energi sebagaimana terjadi pada komoditas lain. Pengolahan awal biomassa lignoselulosa menggunakan asam sulfat memiliki efisiensi yang tinggi tetapi membutuhkan biaya yang tinggi guna menetralisisr produk samping yang terbentuk. Pengolahan awal menggunakan air panas bertekanan dan ball mill dapat digunakan untuk memecah struktur dan komposisi lignoselulosa sehingga dapat meningkatkan laju hidrolisis enzim dan meningkatkan hasil gula. Hidrolisis menggunakan multienzim dengan air panas bertekanan dan ball mill masing-masing dapat menghasilkan 210 mg glukosa pada suhu 220°C dan 200 mg glukosa selama 120 menit pada suhu kamar per gram batang kelapa sawit. Kedua proses tersebut ramah lingkungan dan memiliki efisiensi yang tinggi pada produksi bioetanol dari batang kelapa sawit.Kata Kunci: bioetanol, batang kelapa sawit, ball mill, air panas bertekanan, hidrolisis 
SKRINING DAN ANALISIS FITOKIMIA TUMBUHAN OBAT TRADISIONAL MASYARAKAT KABUPATEN BIMA Azmin, Nikman; Rahmawati, Anita
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1113.415 KB) | DOI: 10.29122/jbbi.v6i2.3504

Abstract

Phytochemical Screening and Analysis of Traditional Herbal Medicines of Bima DistrictBima people use medicinal plants for traditional medicine which is local wisdom that must be preserved. Thus, the phytochemical content of these plants needs to be studied. This study aims to determine the secondary metabolite content of traditional medicinal plants in order to enrich biotechnology and pharmacological data. Thus, the data can be used as a reference in healing diseases and managing various medicinal plants based on community welfare and environmental sustainability for the people of Bima regency. The method used is a detailed interview and a field survey followed by phytochemical screening to determine the content of compounds in plants. From the results of this study, as many as 17 types of medicinal plants have been used as traditional medicine. The plant parts used are leaves, stems, flowers, roots, rhizomes, fruit, gum or lenders with 33% weed habitus followed by herbs (29%), trees (29%), and shrubs (9%). The compounds identified in these medicinal plants are flavonoids, alkaloids, steroids, terpenoids, saponins, and tannins.Keywords: Bima regency; local wisdom; medicinal plants; phytochemicals; secondary metabolitesABSTRAKMasyarakat Bima memanfaatkan tumbuhan obat untuk pengobatan tradisional yang merupakan kearifan lokal yang harus dipertahankan, sehingga kandungan fitokimia dari tumbuhan-tumbuhan ini perlu diteliti. Penelitian ini bertujuan untuk mengetahui kandungan metabolit sekunder tumbuhan obat tradisional guna memperkaya data bioteknologi dan farmakologi. Dengan demikian, data tersebut dapat dijadikan acuan dalam penyembuhan penyakit dan pengelolaan berbagai tumbuhan obat berbasis kesejahteraan masyarakat dan kelestarian lingkungan bagi masyarakat kabupaten Bima. Metode yang digunakan adalah wawancara secara rinci dan survei lapangan dilanjutkan dengan skrining fitokimia untuk mengetahui kandungan senyawa dalam tumbuhan. Dari hasil penelitian ini diketahui sebanyak 17 jenis tumbuhan obat yang telah dimanfaatkan sebagai obat tradisional. Bagian tumbuhan yang digunakan berupa daun, batang, bunga, akar, rimpang, buah, getah atau lender dengan jenis habitus 33% gulma diikuti oleh herbal (29%), pohon (29%), dan perdu (9%). Sedangkan senyawa yang teridentifikasi dalam tumbuhan obat tersebut yaitu flavonoid, alkaloid, steroid, terpenoid, saponin, dan tannin.
Preface JBBI Vol 2, No 2, December 2015: Foreword and Acknowledgement Tajuddin, Teuku
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 2 No. 2 (2015): December 2015
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.589 KB) | DOI: 10.29122/jbbi.v2i2.1062

Abstract

Back Cover JBBI Vol 4, No 2, December 2017 Sriherwanto, Catur
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 4 No. 2 (2017): December 2017
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (83.157 KB) | DOI: 10.29122/jbbi.v4i2.2614

Abstract

OPTIMASI PERMUKAAN RESPONS MEDIUM FERMENTASI Streptomyces prasinopilosus SEBAGAI ANTIFUNGI TERHADAP PATOGEN Ganoderma boninense Sunaryanto, Rofiq; Nurani, Diana
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1326.321 KB) | DOI: 10.29122/jbbi.v6i2.3231

Abstract

Response Surface Optimization of Medium Fermentation for Streptomyces prasinopilosus as An Antifungal against Ganoderma boninenseGanoderma boninense is one of the pathogenic fungi that cause basal stem rot (BPB) on oil palm plants. This research aims to study the effect of carbon sources, nitrogen sources, and minerals on the production of Streptomyces prasinopilosus active compounds. Lactose, yeast extract, and minerals are medium components that show a real influence on the production of S. prasinopilosus active compounds. Optimization of the factors that have significant influence was predicted by the second-order model, statistically through a central composite design (CCD). The highest S. prasinopilosus active compound production, with a medium composition of 44.77 g L-1 lactose, 13.02 g L-1 yeast extract, and 15.95 mL L-1 mineral solution, was predicted by the quadratic model to reach 32269366.338 peak area unit on high-performance liquid chromatography (HPLC). The verification of the mathematical model of the production of the active compounds through experiments in the laboratory was 27,203,907.310 peak area unit. This result was 15.7% lower compared to the result of the quadratic model. Optimization increased S. prasinopilosus active compound 9-fold compared to that before optimization.Keywords: active compound; G. boninense; optimization; RSM; S. prasinopilosus ABSTRAKGanoderma boninense merupakan salah satu jamur patogen yang menyebabkan penyakit busuk pangkal batang atau biasa disebut BPB pada tanaman kelapa sawit. Penelitian bertujuan mempelajari pengaruh sumber karbon, sumber nitrogen, dan mineral terhadap produksi senyawa aktif S. prasinopilosus. Laktosa, yeast extract, dan mineral adalah komponen medium yang menunjukkan pengaruh nyata terhadap produksi senyawa aktif S. prasinopilosus. Optimasi terhadap faktor yang berpengaruh nyata diprediksi dengan model orde dua melalui rancangan statistis central composite design (CCD). Produksi senyawa aktif S. prasinopilosus tertinggi diprediksi oleh model kuadratik mencapai 32269366,338 luasan puncak kromatografi cair kinerja tinggi (KCKT) dengan komposisi medium laktosa 44,77 g L-1, yeast extract 13,02 g L-1, dan larutan mineral 15,95 mL L-1. Verifikasi model matematis produksi senyawa aktif yang dihasilkan melalui percobaan di laboratorium adalah sebesar 27.203.907,310 luasan puncak kromatogram KCKT. Hasil ini lebih rendah 15,7% dibandingkan dengan model kuadratik hasil optimasi. Optimasi meningkatkan senyawa aktif S. prasinopilosus 9 kali lipat dibandingkan sebelum optimasi.
Appendix JBBI Vol 3, No 1, June 2016: Keyword Index and Author Index Sriherwanto, Catur
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 3 No. 1 (2016): June 2016
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (350.169 KB) | DOI: 10.29122/jbbi.v3i1.1514

Abstract

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