cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota adm. jakarta pusat,
Dki jakarta
INDONESIA
Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
JBBI, Indonesian Journal of Biotechnology & Bioscience, is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, as well as life sciences in general. Initiated at the then Biotech Centre, the journal is published by the Laboratory for Biotechnology, the Agency for the Assessment and Application of Technology, BPPT.
Arjuna Subject : -
Articles 542 Documents
 51   52   53   54   55 
INCREASING RECOMBINANT PENICILLIN G ACYLASE PRODUCTION: GENETIC, PROTEIN ENGINEERING, AND PRODUCTIVITY IMPROVEMENT Dini Achnafani; Gabriela Christy Sabbathini; Sri Rezeki Wulandari; Maria Ulfah; Nurul Apsari Aji; Niknik Nurhayati; Haniyya Haniyya; Is Helianti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022): December 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (548.211 KB) | DOI: 10.29122/jbbi.v9i2.5481

Abstract

ABSTRACT B-lactam derived antibiotics are the most used globally for treatment against different infections caused by pathogenic bacteria and comprises 65% of the world antibiotics. Recently, penicillin G acylase (PGA) is used as biocatalyst for those B-lactam antibiotics production by which 6-aminopenicillanic acid (6-APA) or 7-aminodeacetoxycephalosporanic acid (7-ADCA) as the building blocks is produced. Commercialized PGA from native microbial resources are still limited to E. coli. Therefore, genetic engineering approach such as cloning and expression in other microbial hosts were assessed to enhance bacterial strains that produce PGA. However, such improvement could increase immature precursors accumulation and lowering the enzyme yield, activity, or stability. This review focus on the review of PGA recombinant produced by several microbial host, their expression levels, and improvement achieved by some modification such as replacement of signal peptide and promoter continued to protein engineering to utilize the enzymes in synthetizing amoxicillin rather than to hydrolyses Penicillin G.     ABSTRAK Antibiotik turunan B-laktam adalah kelompok antibiotik yang paling banyak digunakan dalam pengobatan infeksi bakteri patogenik dan mencakup 65% dari penggunaan antibiotik di seluruh dunia. Enzim Penisilin G Asilase (PGA) digunakan sebagai biokatalis dalam produksi senyawa bahan baku antibiotik B-laktam berupa asam-6-aminopenisilinat (6-APA) dan asam-7-aminodeasetoksisefalosporanat (7-ADCA). Enzim PGA komersial masih sangat terbatas dan lebih banyak bersumber dari E. coli. Peningkatan produksi PGA dapat dilakukan melaui rekayasa genetika seperti kloning dan ekspresi PGA dengan menggunakan mikroorganisme lain. Namun demikian, rekayasa tersebut dapat menyebabkan akumulasi prekursor dalam sel yang mengakibatkan rendahnya perolehan enzim yang didapat, maupun rendahnya aktivitas dan stabilitas enzim. Tinjauan ini berfokus pada potensi peningkatan produksi PGA rekombinan dari berbagai sel mikroorganisme yang dapat dicapai melalui modifikasi sekuens sinyal peptida dan promoter hingga rekayasa protein dengan tujuan mendapatkan enzim PGA untuk sintesis antibiotik amoksisilin daripada hidrolisis PGA.
THE EFFECT OF LIQUID TOFU WASTE AND GROWING MEDIA ON THE GROWTH AND YIELD OF LONG BEANS (Vigna sinensis L.) Nindi Fauziah; M. Idris
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022): December 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (484.743 KB) | DOI: 10.29122/jbbi.v9i2.5492

Abstract

ABSTRACT Long bean is a leguminous plant that has the potential to be developed into productive plants. Tofu liquid waste organic fertilizer and planting media are needed to meet nutrient needs, improve the physical and chemical properties of the soil. This study aims to understand the effect of liquid tofu waste, growing media, and their combined interaction on the growth and yield of long beans. This study used a factorial randomized block design (RBD) with 3 replications, 3 treatments of liquid tofu waste of 0 mL (T0), 150 mL (T1), and 300 mL (T2), and 3 planting media, namely cow manure compost + rice husk charcoal (M1), cow manure compost + cocopeat (M2), and cow manure compost + chopped fern (M3). If they had a significant effect, a Tukey's test at a 5% probability level would be performed. The parameters observed included the chlorophyll a and b, net assimilation rate, relative growth rate, height, number of flowers, pod length, and wet fruit weight of long beans. The results showed that the T1 and M3 treatments significantly affected chlorophyll a and b, height, number of flower, and pod length. The T1 and M2 treatments significantly affected the wet fruit weight of long beans.The T2 and M2 treatments significantly affected the net assimilation rate. Finally, the T2 and M3 treatments significantly affected the relative growth rate. There was no interaction effect found between liquid tofu waste and growing media on all research parameters.   ABSTRAK Kacang panjang adalah tanaman polong-polongan yang memiliki potensi untuk dikembangkan menjadi tanaman produktif. Diperlukan pupuk organik limbah cair tahu dan media tanam untuk memenuhi kebutuhan unsur hara, memperbaiki sifat fisik dan kimia tanah. Penelitian ini bertujuan mengetahui pengaruh pemberian limbah cair tahu, media tanam dan juga interaksi kombinasi terhadap pertumbuhan dan hasil tanaman kacang panjang. Penelitian menggunakan rancangan acak kelompok (RAK) faktorial dengan 3 ulangan, 3 perlakuan limbah cair tahu 0 mL (T0), 150 mL (T1), 300 mL (T2), dan terdiri dari 3 media tanam yaitu kompos kotoran sapi + arang sekam padi (M1), kompos kotoran sapi + cocopeat (M2), kompos kotoran sapi + pakis cacah (M3). Parameter yang diamati yaitu klorofil a, b, laju asimilasi bersih, laju pertumbuhan relatif, tinggi tanaman, jumlah bunga, panjang buah, dan bobot basah buah. Hasil penelitian menunjukkan perlakuan T1 dan M3 berpengaruh sangat nyata terhadap klorofil a, b, tinggi tanaman, jumlah bunga dan panjang buah. Perlakuan T1 dan M2 berpengaruh sangat nyata terhadap bobot basah buah. Sedangkan perlakuan T2 dan M2 berpengaruh sangat nyata terhadap laju asimilasi bersih. Sedangkan perlakuan T2 dan M3 berpengaruh sangat nyata terhadap laju pertumbuhan relatif. Tidak ada pengaruh interaksi antara limbah cair tahu dan media tanam terhadap seluruh parameter penelitian.
MORPHOLOGICAL AND TEXTURAL FEATURE EXTRACTIONS FROM FUNGI IMAGES FOR DEVELOPMENT OF AUTOMATED MORPHOLOGY-BASED FUNGI IDENTIFICATION SYSTEM R. Putri Ayu Pramesti; Muhamad Rodhi Supriyadi; Aulia Haritsuddin Karisma; Muhammad Reza Alfin; Mukti Wibowo; Bayu Rizky Maulana; Gilang Mantara Putra; Josua Geovani Pinem; Umi Chasanah; Kristiningrum Kristiningrum; Ariza Yandwiputra Besari; Avi Nurul Oktaviani; Dyah Noor Hidayati; Dewi H Budiarti; Jemie Muliadi; Danang Waluyo; Anto Satriyo Nugroho
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022): December 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (478.633 KB) | DOI: 10.29122/jbbi.v9i2.5512

Abstract

ABSTRACT Due to widely varied microscopic shapes, fungal classification can be performed based on their morphological features. In morphology-based identification process, feature extraction takes an important role to characterize each fungal type. Previous studies used feature extraction of fungal images to detect the presence of fungal. In this study, morphological and textural features were extracted to classify three types of fungi: Aspergillus, Cladosporium and Trichoderma. Geometry and moment were used as morphological features. To perform textural feature extraction, the local binary pattern (LBP) and gray level co-occurrence matrix (GLCM) feature extraction method were used. We compared the implemented feature extraction methods in order to get the best classification result. The result showed that geometrical features has the accuracy of 65%, higher than that of LBP (60%), GLCM (45%), and moment accuracy (55%). This suggested that geometric features is important for fungal classification based on their morphology.   ABSTRAK Karena bentuk mikroskopisnya yang sangat bervariasi, klasifikasi jamur dapat dilakukan berdasarkan ciri morfologisnya. Dalam proses identifikasi berbasis morfologi, ekstraksi ciri berperan penting untuk mengkarakterisasi setiap jenis jamur. Penelitian-penelitian yang dilakukan sebelumnya melakukan ekstraksi ciri citra jamur untuk mendeteksi keberadaan jamur. Dalam penelitian ini, fitur morfologi dan tekstur diekstraksi untuk mengklasifikasikan tiga jenis jamur: Aspergillus, Cladosporium dan Trichoderma. Geometri dan momen digunakan sebagai ciri morfologi. Untuk melakukan ekstraksi ciri tekstur, digunakan metode ekstraksi ciri local binary pattern (LBP) dan gray level co-occurrence matrix (GLCM). Kami membandingkan metode ekstraksi fitur yang diterapkan untuk mendapatkan hasil klasifikasi terbaik. Hasil penelitian menunjukkan bahwa fitur geometri memiliki akurasi 65%, lebih tinggi dari LBP (60%), GLCM (45%), dan akurasi momen (55%). Ini menunjukkan bahwa fitur geometris penting untuk klasifikasi jamur berdasarkan morfologinya.
EFFECTS OF SEED COATING USING PLANT-BENEFICIAL MICROBES ON THE GERMINATION OF Centrosema pubescens SEEDS Indri Handayani; Farida Rosana Mira; Zhafira Amila Haqqa; Nia Asiani; Abdul Wahid; Mahmud Sugianto; R Bambang Sukmadi; Bedah Rupaedah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022): December 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (667.654 KB) | DOI: 10.29122/jbbi.v9i2.5541

Abstract

ABSTRACT Centrosema pubescens is a legume plant that is commonly used as animal feed, cover crop, and one of the plants used to reclaim critical land. This study was conducted to increase the germination of C. pubescens seeds by coating the seeds using a coating material enriched with a consortium of microbes consisting of nitrogen-fixing bacteria, phytohormones-producing bacteria, phosphate-solubilizing bacteria, and arbuscular mycorrhizal fungi. Germination test on control, seed coating, and microbial seed coating was carried out using the top of the sand method for 28 days. Observational data were tested using ANOVA statistics and LSD tests. Results of the research showed the moisture content of the seeds was 12.45%, the purity of the seeds was 95.11% and the weight of 1000 seeds was 23.74 g. The germination test denoted that the number of normal germinated seeds in seed coating and microbial seed coating was significantly different from the control. In addition, dead seeds in microbial seed coating treatment had the lowest value and were significantly different from other treatments. These results indicate that microbial seed coating can increase seed germination and considerably reduce seed death due to seed-borne pathogens.   ABSTRAK Centrosema pubescens adalah tanaman kacang-kacangan yang dimanfaatkan sebagai pakan ternak, penutup tanah dan salah satu tanaman yang digunakan di lahan kritis. Penelitian ini dilakukan untuk meningkatkan perkecambahan benih C. pubescens dengan melakukan pelapisan benih yang diperkaya dengan bakteri penambat nitrogen, bakteri penghasil fitohormon, bakteri pelarut fosfat dan cendawan mikoriza arbuskular.  Uji Daya berkecambah dilakukan pada benih kontrol, benih berlapis dan benih berlapis mikroba menggunakan metode penanaman benih di atas pasir selama 28 hari. Data pengamatan dianalisis menggunakan ANOVA dan uji lanjut LSD. Hasil penelitian menunjukkan kadar air benih C. pubescens 12,45%, kemurnian benih 95,11% dan bobot 1000 benih 23,74 g. Daya berkecambah benih menunjukkan perlakuan benih berlapis mikroba berbeda signifikan dengan kontrol. Persentase benih mati pada perlakuan benih berlapis mikroba memiliki nilai paling kecil dan berbeda nyata terhadap perlakuan lainnya. Hal ini mengindikasikan bahwa pelapisan benih menggunakan mikroba dapat meningkatkan persentase perkecambahan benih dan mengurangi jumlah benih mati akibat patogen bawaan benih.
PRODUCTION OF HUMAN INSULIN PRECURSOR IN A 10 LITER BIOREACTOR USING A METHANOL PULSE FEEDING Dian Japany Puspitasari; Hendi Saleh Permana; Rudiyono Rudiyono; Anis Herliyati Mahsunah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022): December 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (615.869 KB) | DOI: 10.29122/jbbi.v9i2.5552

Abstract

ABSTRACT Indonesia as a country that had 19.5 million diabetic patients in 2021 is still importing raw materials of human insulin. The development of human insulin production process is needed to support the government’s objective to be independent in medicine aspect. Human insulin precursor (HIP) expressed from Pichia pastoris X33/pD902-IP had been developed and optimized in small-scale cultivation. However, the scaling up in bioreactor 10 L has not been studied. Using a 10 L-bioreactor the fermentation of P. pastoris X33/pD902-IP was developed. Fermentation was done in 120 hours using a basal salt medium (half concentration) for the vegetative and induction media. To induce HIP expression, methanol is fed by pulse strategy with a gradient concentration of 1-3% (v/v) for 48 hours. The dry cell weight (DCW) and HIP titers were 72 g/L and 286 mg/L, respectively. This development is the first fermentation of HIP expressed by P. pastoris X33/pD902-IP in a 10 L-bioreactor.   ABSTRAK Indonesia sebagai negara dengan penderita diabetes 19,5 juta orang pada tahun 2021 masih mengimpor bahan baku insulin manusia. Pengembangan proses produksi insulin manusia diperlukan dalam rangka mendukung tujuan pemerintah untuk mandiri di bidang produksi bahan baku obat. Ekspresi prekursor insulin manusia oleh Pichia pastoris X33/pD902-IP telah dikembangkan dan dioptimalkan dalam skala kultur kocok. Akan tetapi, produksinya pada bioreaktor 10 L belum dilakukan. Untuk itu, fermentasi P. pastoris X33/pD902-IP dipelajari pada bioreaktor 10 L. Fermentasi dilakukan selama 120 jam dengan menggunakan media garam basal (setengah konsentrasi). Untuk menginduksi ekspresi HIP, metanol diberikan dengan gradien konsentrasi 1-3% (v/v) secara berkala selama 48 jam. Berat sel kering dan titer HIP masing-masing adalah 72 g/L dan 286 mg/L. Proses ini merupakan fermentasi pertama HIP yang dihasilkan P. pastoris X33/pD902-IP pada skala bioreaktor 10 L.
VOLATILE COMPOUND ANALYSIS OF AROMATIC RICE MUTANT LINES USING HS-SPME/GC-MS Muh Riadi; Andi Muliarni Okasa; Rinaldi Sjahril; Meta Mahendradatta; Rafiuddin Rafiuddin
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 2 (2022): December 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (445.198 KB) | DOI: 10.29122/jbbi.v9i2.5555

Abstract

ABSTRACT Volatile compound is one of the key factors for aromatic components of rice. This study aimed to identify the key aroma components and their relationship with plant productivity in the Pare Bau variety mutant lines and its wild type. Volatile extraction was carried out using the headspace solid-phase microextraction (HS-SPME) method and analysed by the Gas Chromatography-Mass Spectroscopy (GC-MS) instrument. The results of the identification of volatile compounds showed a total of 224 compounds in the mutant lines and wild type. However, only 14 compounds were suspected as key aroma compounds in Pare Bau rice, namely 2-Acetyl-1-pyrroline, Indole, 1-Octanol, 1-Octen-3-ol, 2,4-Nonadienal, (E,E)-, Octanal, 2-Nonenal, (E)-, 2-Octenal, (E)-, Decanal, Hexanal, Nonanal, Furan, 2-pentyl-, toluene and vanillin. The results on aroma compounds of mutant lines using principal component analysis showed that there were differences in the main characteristics of several strains and wild type. There was no relationship between increasing volatile concentration and plant productivity, vice versa.   ABSTRAK Senyawa volatil merupakan salah satu faktor kunci sebagai komponen aromatik beras. Penelitian ini bertujuan untuk mengidentifikasi faktor kunci sebagai komponen aroma dan hubungannya dengan produktivitas pada galur mutan dan Pare Bau (genotipe tetua). Ekstraksi senyawa volatil dilakukan dengan metode headspace solid-phase microextraction (HS-SPME) dan dianalisis dengan instrumen Gas Chromatography-Mass Spectroscopy (GC-MS). Hasil identifikasi senyawa volatil menunjukkan sejumlah 224 senyawa yang terdapat dalam galur mutan dan genotipe tetuanya. Namun, hanya 14 senyawa yang diduga sebagai senyawa aroma utama pada beras Pare Bau, yaitu 2-Acetyl-1-pyrroline, Indole, 1-Octanol, 1-Octen-3-ol, 2,4-Nonadienal, (E,E)-, Oktanal, 2-Nonanal, (E)-, 2-Oktenal, (E)-, Dekanal, Heksanal, Nonanal, Furan, 2-pentil-, toluen dan vanilin. Hasil analisis senyawa aroma galur mutan dengan analisis komponen utama menunjukkan adanya perbedaan karakteristik utama dari beberapa galur dengan genotipe tetuanya. Tidak terdapat hubungan antara peningkatan konsentrasi senyawa volatil dengan produktivitas tanaman.
LIPID PRODUCTION FROM BR. 2.2 OLEAGINOUS FUNGAL ISOLATE USING ACETATE, GLYCEROL, AND MOLASSES AS CARBON SOURCES Chikmatul Alifiyatin; Salma Darmayanti; Wanny Firdasia; Miftahul Ilmi
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023): Juni 2023
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Microorganisms that accumulate more than 20% of their dry cell weight as lipids are called oleaginous microorganisms. Oleaginous microorganisms can grow well on various carbon sources other than glucose. These non-glucose alternative carbon sources could potentially reduce high biofuel  manufacturing  costs. BR.  2.2  isolate  is an oleaginous fungus that accumulates 0.62 g L-1 lipids using  glucose as a carbon  source.  This study aims to determine the effect of acetate, glycerol, molasses, and C/N ratios on lipid accumulation of the BR.2.2 isolate. The highest lipid produced by the BR. 2.2 isolate using acetate is 0.196 g L-1 at a C/N ratio of 400, 0.229 g L-1 at a C/N ratio of 225 using glycerol, and 1.97 g L-1 at a C/N ratio of 25 using molasses in 144 hours of incubation. The results revealed that the accumulation of lipids increased with the rising acetate and glycerol C/ N ratios and incubation period. Meanwhile, the accumulation of lipids decreased  with increasing molasses C/N ratio.
A REVIEW OF NANOCELLULOSE SYNTHESIS METHODS AND ITS APPLICATION Fuad Dimar Fauzi; Hafidz Dhany Wicaksono; Joko Waluyo
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023): Juni 2023
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v10i1.5349

Abstract

Nanocellulose is a type of cellulose that is being widely developed to replace petroleum-based polymers. This material possesses biocompatible properties, is abundant in nature, and is eco-friendly due to its biodegradability, sustainability, and non-toxic nature. Various nanocellulose synthesis methods are employed, including acid hydrolysis, alkaline, mechanical and biological treatments, as well as ionic liquid and deep eutectic solvent methods. The choice of synthesis method greatly influences the particle size and crystallinity of the resulting nanocellulose; hence further investigation is needed to determine the effectiveness of these methods. Nanocellulose finds applications in various fields, such as films, polymers, cosmetics, medical fuels, and energy storage. Among the different nanocellulose synthesis methods, ionic liquid and deep eutectic solvent (DES) methods have environmentally safe waste with better temperature, time, and diameter control compared to other methods. However, the DES method is currently preferred over the ionic liquid method due to the possibility of separating the lignin waste from the solvent.
ROOT INDUCTION ON THE SHOOTS OF RUBBER TREE (Hevea brasiliensis) CLONE PB 260 THROUGH EX VITRO TECHNIQUE Yusuf Sigit Ahmad Fauzan; . Dasumiati; Dewi Rizkiani; Syofi Rosmalawati
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023): Juni 2023
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v10i1.5363

Abstract

Rubber tree (Hevea brasiliensis) is a crucial plantation commodity for Indonesia's national development. However, the country's rubber productivity lags behind that of other rubber-producing nations. To address this, propagating rubber plants from superior clones through ex-vitro techniques becomes essential.. This study aimed to determine the optimal concentrations of IBA and NAA for root induction in rubber tree shoot explants. A factorial complete randomized design was employed, with the first factor being IBA concentration (0, 100, 200, 300, 400, and 500 ppm) and the second factor being NAA concentration (0, 50, 100, and 150 ppm). The results revealed that the percentage of surviving explants after induction with IBA and NAA did not significantly differ. The rooted explants were obtained using the following treatments: IBA 200 ppm and NAA 0 ppm, IBA 200 ppm and NAA 50 ppm, IBA 300 ppm and NAA ppm, and IBA 400 ppm and NAA 0 ppm. Various concentrations of IBA and NAA did not significantly affect root formation in rubber tree shoot explants.
OPTIMIZATION OF INTRANASAL COVID-19 VACCINE FORMULATION WITH Lactococcus lactis pNZ HCR BACTERIA AS VECTOR IN LIQUID AEROSOL PREPARATION Juan Freddy; Christopher Kuncoro Johan; Samuel Aryo Wicaksono; Daffa Rizky; Shafira Gita Eka Pritayanti; Oktavia Rahayu Adianingsih; Valentina Yurina
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023): Juni 2023
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v10i1.5550

Abstract

Vaccination is an effective method to suppress COVID-19 transmission, but injection-based vaccination is less effective due to its inability to induce mucosal immunity. This study aimed to determine the effects of vaccine formulations on bacteria viability and antigen expression to find the optimal formulation. Three intranasal preparation formulations (F1, F2, and F3) were created with different ingredient compositions, along with a control. Physicochemical tests were conducted on day 0 and day 14 to assess bacterial viability, and antigen expression was evaluated using the western blot method. Formula 2, containing sodium alginate (0.615%), trehalose (4.125%), polyvinyl alcohol (0.1%), and calcium chloride (5%), exhibited the best viability test results, although no significant differences were observed among the groups. The study concluded that variations in composition concentrations could affect bacterial stability, with Formula 2 showing the best results in terms of bacteria viability and antigen expression up to 14 days after formulation.

Page 53 of 55 | Total Record : 542

 51   52   53   54   55 

Filter by Year

2014 2023


Reset
Filter By Issues
All Issue Vol. 10 No. 1 (2023): Juni 2023 Vol. 9 No. 2 (2022): December 2022 Vol. 9 No. 1 (2022): June 2022 Vol. 8 No. 2 (2021): December 2021 Vol. 8 No. 1 (2021): June 2021 Vol. 7 No. 2 (2020): December 2020 Vol. 7 No. 1 (2020): June 2020 Vol. 6 No. 2 (2019): December 2019 Vol. 6 No. 1 (2019): June 2019 Vol 6, No 1 (2019): June 2019 Vol. 5 No. 2 (2018): December 2018 Vol 5, No 2 (2018): December 2018 Vol 5, No 1 (2018): June 2018 Vol. 5 No. 1 (2018): June 2018 Vol. 4 No. 2 (2017): December 2017 Vol 4, No 2 (2017): December 2017 Vol. 4 No. 1 (2017): June 2017 Vol 4, No 1 (2017): June 2017 Vol. 3 No. 1 (2016): June 20160 Vol 3, No 1 (2016): June 20160 Vol. 3 No. 2 (2016): December 2016 Vol 3, No 2 (2016): December 2016 Vol. 3 No. 1 (2016): June 2016 Vol 3, No 1 (2016): June 2016 Vol. 2 No. 2 (2015): December 20150 Vol 2, No 2 (2015): December 20150 Vol. 2 No. 1 (2015): June 20150 Vol 2, No 1 (2015): June 20150 Vol 2, No 2 (2015): December 2015 Vol. 2 No. 2 (2015): December 2015 Vol 2, No 1 (2015): June 2015 Vol. 2 No. 1 (2015): June 2015 Vol. 1 No. 1 (2014): December 20140 Vol 1, No 1 (2014): December 20140 Vol 1, No 1 (2014): December 2014 Vol. 1 No. 1 (2014): December 2014 More Issue