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INDONESIA
Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
JBBI, Indonesian Journal of Biotechnology & Bioscience, is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, as well as life sciences in general. Initiated at the then Biotech Centre, the journal is published by the Laboratory for Biotechnology, the Agency for the Assessment and Application of Technology, BPPT.
Arjuna Subject : -
Articles 542 Documents
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INHIBITORY ACTIVITY OF INDONESIAN MICROBIAL EXTRACTS AGAINST PROLIFERATION OF DLD-1 COLORECTAL CANCER CELL LINE Ariza Yandwiputra Besari; Danang Waluyo; Tinta Komariyah; Erwahyuni Endang Prabandari
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023): Juni 2023
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v10i1.5590

Abstract

Colorectal cancer (CRC) is the second deadliest cancer in the world. Several anti-cancer agents are currently used for the clinical treatment of CRC. However, toxicity and drug resistance pose significant challenges in CRC chemotherapy. On the other hand, microbe-derived natural products have been explored as a source for the development of anti-cancer therapeutic agents. This study aimed to examine the potential of the microbial library in BioMCC (Biotech Center-BPPT Microbial Culture Collection) as a source for anti-cancer drug discovery. Among the 720 fungal extracts tested, 60 extracts (8.3%) showed inhibitory activity against the proliferation of the colorectal carcinoma DLD-1 cell line, while not affecting Vero cells (African green monkey kidney normal cell line). One of these active extracts was derived from the fungus Sporothrix sp. BioMCC-f.T.7716. Although the inhibitory mechanism of this extract against the proliferation of the DLD-1 cell line could not be determined, this study clearly demonstrated the potential use of Indonesian microbial extracts as a source for the discovery of anti-cancer agents.
ENZYMATIC DEGUMMING USING XYLANASE AND PECTINASE TO IMPROVE BRIGHTNESS AND FINENESS QUALITY OF RAMIE FIBER (Boehmeria nivea L.) AS TEXTILE RAW MATERIAL Kharis Yohan Abidin; Niknik Nurhayati; Dewi Nandyawati; Gabriela Christy Sabbathini
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023): Juni 2023
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

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Abstract

Ramie (Boehmeria nivea L.) fiber is an alternative to cotton fiber, and the degumming process is crucial for preparing it as a textile raw material. This study investigates the enzymatic degumming of ramie fiber using a combination of xylanase enzyme from Bacillus halodurans CM1 and commercial pectinase enzyme. The objective is to assess the impact of enzymatic degumming on the physical properties (weight loss, whiteness index, tenacity, elongation, and fineness) of ramie fiber. The degumming process was conducted in a shaker incubator at a temperature of 50 °C, pH 9, and 150 rpm. The degumming treatment involved 3% v/v xylanase, 1% v/v pectinase, 1% v/v surfactant, and 0.05% v/v NaClO2. The results show that the bleaching treatment (S6) resulted in higher fiber weight loss (9.52%), whiteness index (87.87%), tenacity (20.08 g/Tex), and fineness (1.05 denier) compared to the non-bleaching treatment.
OPTIMIZATION OF PRIMARY SOMATIC EMBRYOGENESIS INDUCTION OF COCOA (Theobroma cacao L.) THROUGH THE ADDITION OF PHENOLIC COMPOUND SUPPRESSANT MATERIALS Putri Oktaviana Priandani; Sulistyani Pancaningtyas; Mohammad Ubaidillah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023): Juni 2023
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v10i1.5612

Abstract

Cocoa primary somatic embryogenesis is a propagation technique that produces primary somatic embryos from cocoa flower parts. The percentage of primary somatic embryo formation in cocoa tends to be low due to the significant secretion of phenolic components in flower explants thereby reducing the conversion rate of explants into embryos. This is indicated by browning that occurs in explants or culture media. The purpose of this study was to determine the optimization of primary somatic embryogenesis induction of cocoa through the administration of several types of phenolic compound suppressor materials. Staminodia explants were grown on DKW media with factorial treatment: the addition of various phenolic compounds suppressing materials (control; 300 mg L-1 PVP; 15 mg L-1 silver nitrate; 12 mg L-1 melatonin; 300 mg L-1 ascorbic acid) and cultivar type (MCC -02 and ICCRI-09). The results showed that the treatment of each material gave a different response in suppressing phenolic compounds in each cultivar. Melatonin treatment gave the lowest percentage of browning intensity so it also affected the best results on morphology, callus size, wet weight, and parameters of the percentage of live explants.
SYRINGIC ACID AND PHENAZINE PRODUCED BY AN ENDOPHYTIC Pseudomonas aeruginosa STRAIN G-111-0317 AND THEIR ACTIVITIES AGAINST Ganoderma boninense Bedah Rupaedah; Nurlaila Nurlaila; Anisa Lutfia
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023): Juni 2023
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v10i1.5628

Abstract

Syringic acid and phenazine possess antibiotic and antifungal properties, and have demonstrated effectiveness in inhibiting the colonization of Ganoderma boninense on oil palm plants. Some bacteria, including Pseudomonas aeruginosa strain G-111-0317, are capable of producing syringic acid and phenazine. In this study, the culture extract of this bacterial strain was obtained from healthy oil palm plants growing in G. boninense-infected areas at Oil Plant Plantation Pematang Siantar, North Sumatra. The strain was cultured on Nutrient Broth (NB) medium, and the resulting culture filtrate was extracted using ethyl acetate (EtOAc) and concentrated under vacuum. The putative compounds were identified by LC-MS, employing syringic acid and phenazine as reference standards. Antifungal activity against G. boninense in vitro was observed in the EtOAc extract obtained after 8 hours and 24 hours of fermentation, with the 8-hour extract demonstrating the highest activity. These compounds hold promising potential as active agents in inhibiting basal stem rot disease in oil palm plants.
ISOLATION OF BACTERIA AS A BIOREMEDIATION AGENT FOR RECLAMATION OF MERCURY-CONTAMINATED SOILS Muhammad Idris; Rizki Amelia Nasution; Ulfayani Mayasari
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023): Juni 2023
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

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Abstract

Gold mining commonly involves the use of mercury, which generates hazardous and toxic mercury-based waste, leading to soil contamination and adverse effects on human health. Bioremediation has been explored as a potential solution to this issue. This study aimed to identify indigenous microbial species with potential as bioremediation agents, assess their ability to influence chemical properties, and evaluate their effectiveness in reducing mercury content. The research was conducted in five stages: isolation of native bacteria, testing the potential of microorganisms, assessing the ability to reduce mercury, conducting soil tests, and identifying bacterial characteristics through microscopic and biochemical analyses. The results revealed three types of indigenous microbes, namely Pseudomonas, Neisseria, and Klebsiella bacteria, with the highest potential as bioremediation agents. These bacterial isolates were found to enhance the availability of phosphorus in the soil, maintain soil pH, but had no effect on total soil nitrogen. Furthermore, the bacterial isolates exhibited the ability to reduce mercury content after treatment with NA isolates.
MUTATION DETECTION OF MULTIDRUG-RESISTANT TUBERCULOSIS BY RT-PCR METHOD AS THE DIAGNOSTIC TOOL OF MDR-TB Titta Novianti; alfero Putra Iryanto; feby feby; callista marsya; putri mega utami; febriana dwi wahyuni; henny saraswati; seprianto; adri nora; roaslein putri; nie nie; sabar pambudi
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023): Juni 2023
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v10i1.5653

Abstract

Eight percent of tuberculosis (TB) cases worldwide are resistant to rifampicin, with mutations occurring in the rpoB and katG genes. It is necessary to develop a specific multidrug-resistant (MDR) diagnostic technique using the RT-PCR method in Indonesia to aid in rapid and accurate diagnosis. In-silico testing using SnapGene software resulted in the design of DNA primers for the katG and rpoB genes, plasmids, and specific probes. This study employed a cross-sectional design using 30 non-MDR-TB and MDR-TB samples from RSUD Sitanala, Tangerang Banten, which were tested for amplification of the katG and rpoB genes using Sybr green RT-PCR. Validity testing was conducted using specific probes for the katG and rpoB genes. The amplification results showed that MDR-TB samples and MDR-TB plasmids required a longer time compared to non-MDR-TB samples and non-MDR-TB plasmids. The Quantification Cycle (Cq) value in non-MDR-TB samples was lower than the Cq value in MDR-TB samples. A t-test revealed a significant difference in Cq values of the rpoB and katG genes between MDR-TB and non-MDR-TB patients (p-value < 0.005). These differences in Cq values indicate that the findings of this study can serve as an initial reference for the development of an RT-PCR-based diagnostic kit for MDR-TB.
OPTIMIZATION OF ENZYME-MICROWAVE ASSISTED EXTRACTION, CHARACTERIZATION AND ANTIOXIDANT ACTIVITY OF POLYSACCHARIDE FROM Ganoderma lucidum Lira Windriawati Listriyani; Sumi Hudiyono; . Rudiyono; Siti Zulaeha; Ahmad Wibisana
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023): Juni 2023
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v10i1.5663

Abstract

Enzyme-Microwave Assisted Extraction (EMAE) is a new process for extracting Ganoderma lucidum polysaccharides (GLPs). Cellic® CTec2 was chosen as an enzyme that assists in microwave extraction. The four variables involved in this study were enzyme concentration (%), enzymatic reaction time (minutes), solvent-to-solid ratio (mL/g), and microwave extraction time (minutes). This study showed that the enzyme concentration and solvent-to-solid ratio had a significant effect on the response in the range studied. Yield extraction of polysaccharides from experiments conducted at optimum conditions showed good agreement with the predictions from the model. The EMAE method showed a higher polysaccharide extraction yield than hot water extraction (HWE) method. GLPs from EMAE method had antioxidant activity of 79.47 ± 0.71% (DPPH) and 0.884 ± 0.013 mM Fe2+/L (FRAP), where these values were higher than those of the HWE method.
MUTATION OF LIPASE-PRODUCING BACTERIAL ISOLATE FROM PALM OIL EFFLUENT FOR FAT HYDROLYSIS ON POME Dadang Suhendar; Ika Rahmatul Layly; Gabriela Christy Sabbathini; Deden Rosid Waltam; Edi Wahjono; Catur Sriherwanto; Haniyya Haniyya
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023): Juni 2023
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v10i1.5665

Abstract

Lipase digunakan untuk menghidrolisis minyak atau lemak yang tersisa dalam limbah cair pabrik kelapa sawit (POME), yang digunakan sebagai bahan baku dalam pembentukan biogas. Isolat bakteri (Bacillus velezensis) yang menunjukkan aktivitas lipase, sebelumnya diperoleh dari Malimping, Pandeglang, Banten, Indonesia, mengalami perlakuan berurutan yaitu iradiasi gamma (1, 2, dan 3 kGy), mutagenesis EMS (ethyl methane sulfonate) (0,5% v/v), dan NTG (N-methyl N-nitrosoguanidine) (1 mg mL-1). Setelah setiap mutasi, aktivitas lipase dari koloni mutan diukur dan dibandingkan dengan strain tipe liar. Hasil penelitian menunjukkan bahwa semua koloni B. velezensis yang bermutasi menunjukkan nilai aktivitas lipase (7,78 ± 0,80 – 9,05 ± 0,23 U mL-1) yang tidak berbeda signifikan dengan isolat tipe liar (8,31 ± 0,01 U mL-1), menunjukkan ketahanan bakteri yang kuat terhadap perlakuan mutagenik. Preparasi lipase kasar secara efektif menghidrolisis POME, mengakibatkan penurunan nilai COD (dari 131.450 ppm menjadi 88.450 ppm) dan kandungan O&G (dari 41.400 mg L-1 menjadi 5.770 mg L-1) dalam jangka waktu 72 jam.
SCREENING AND CHARACTERIZATION OF SOIL FUNGAL ISOLATES FOR INHIBITING THE GROWTH OF Ganoderma boninense Zhafira Amila Haqqa; Bedah Rupaedah; Indri Handayani; Abdul Wahid; Mahmud Sugianto
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023): Juni 2023
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

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Abstract

Ganoderma boninense, a fungus recognized as a causative agent of basal stem rot and upper stem rot, is primarily found in oil palm plantations (Elaeis guineensis Jack.). This study aimed to identify soil fungal isolates with the greatest potential for inhibiting the pathogenic fungus G. boninense. The research employed curative antagonist testing using in vitro dual culture. Fungal isolates demonstrating the highest inhibition percentages were characterized through macroscopic and microscopic observation, and their hemolysis properties were assessed using blood agar media. Soil fungal isolates FA 3.8 and FA 2.8 exhibited the highest inhibition percentages, reaching 91% and 88%, respectively. Based on morphological characterization at both macroscopic and microscopic levels, FA 3.8 displayed similarities to Trichoderma, while FA 2.8 exhibited similarities to Penicillium. Hemolysis testing results on blood agar media indicated that both isolates exhibited gamma hemolysis or non-hemolysis, as they lacked red blood lysis properties.
Front Cover JBBI Vol 10, No 1, Juni 2023 Teuku Tajuddin
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023): Juni 2023
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

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Abstract

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