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Medical Laboratory Technology Journal

JAK
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Published by Poltekkes Kemenkes Banjarmasin

ISSN : - EISSN : 24610879 DOI : https://doi.org/10.31964/mltj

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Nursing Public Health

The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have achieved in the area of medical laboratory sciences. This Journal particularly focuses on the main problems in the development of the sciences of medical laboratory areas. It covers the parasitology, bacteriology, virology, hematology, clinical chemistry, toxicology, food and drink chemistry, and any sciences that cover sciences of medical laboratory area.

Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan

Articles 193 Documents

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The Reduction of Carbohydrate, Fat and The Increment of Protein Content of Some Nigerian Diets by Traditional Fermentation Ehoche E Elijah; Henry Y Adeyemi
Medical Laboratory Technology Journal Vol. 4 No. 2 (2018): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Fermentation is vital to African food processing. Its effects on the percentage carbohydrate, proteins, lipid and moisture composition of laboratory prototypes of the fermented seed from Parkia biglobosa, (Dawadawa) condiment paste, fermented milk (Nono), corn (Zea mays)-based pap (Akamu), soybean (Glycine max) based-cheese paste (wara) and soy-milk (soymilk). The major macro-nutrient and moisture contents of each food product and their respective substrates were determined using standard methods and compared. The result showed that there was a noticeable fall in the carbohydrate content in the Corn (56.23±9.09 %) as it was converted to Akamu (7 .63±2.67 %) just as was noticed in the fermentation of Nono (11.99±2.67 %) from fresh cow milk (42.3±1.60 %). The similar trend was also found in the fermentation of the lipid-containing soy bean seed (41±7) to soy wara (7.6±2 %) and soymilk (5.6±2.2 %). However, there was an increase in the protein content from the fermentation of Parkia biglobosa seed: 31.62±0.83 - 34.17±3.6 % in Dawadawa and 25.25±0.59 - 37.74±1.8 % in Nono. Moisture contents of the various fermented food products also increased as follows: from 9.00 ±0.01-90.0±0.70 in Akamu; 89.0±0.58 into 92.7±0.98 in Nono, 13.0±0.87 -33±0.01 in Dawadawa paste, and 5.0±0.01 - 39±1.41 % in soy milk and 31 ±1.4 % in soy wara. These show that fermenting foods could reduce their carbohydrate and fat content relatively but increase their protein content. These cannot be overemphasized considering the problem of malnutrition which is prevalent around this part of the world.

Colposcopy results in Smear negative, High-risk HPV positive patients Deha Denizhan Keskin
Medical Laboratory Technology Journal Vol. 4 No. 2 (2018): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Cervix cancer is an HPV (Human papillomavirus) related cancer, and HPV positivity is necessary even if there is no cytology abnormality. We aimed to determine the ratios of 13 high-risk HPV types in cases with high-risk HPV positivity without cervical smear pathology referred to our clinic and to determine the relation of HPV types with age, parity, menopausal status, and abnormal histopathological results. Two hundred forty-one cases included in the study, which referred to us because of HPV positivity and colposcopically biopsied between January 2014 to January 2018. HPV prevalences were investigated. The relationship between HPV types and variables such as age, parity, menopausal status examined. The mean age of 241 patients included in the study was 46,1+8,8. The parity average was 2,4+1,1. Sixty-five of the patients (27%) were postmenopausal. Of the 241 HPV-positive patients, 172 (71,4%) had only high-risk HPV viruses. The frequency ranking of HPV types was as follow; 16, 31, 51, 56, 18, 52, 35, 58, 39, 68, 45, 33 and 59. According to the HPV types, the average ages were as follow; 18 (43,6 years), 33 (40,1 years) and 51 (41,9 years) were younger than the average age. 35 (48,7 years), 39 (48,5 years), 52 (49,1 years) and 68 (51,3 years) were older than the average age. 16 (44,9 years), 31 (47,9 years), 45 (44,3 years), 56 (47,3 years), 58 (46,9 years) and 59 (46,7 years) was similar the average age. There was no significant difference between the parities according to HPV types (2 to 2,7). According to the HPV types, the menopausal state was as follows; 39 (50%), 56 (50%) and 68 (53,8%) mostly observed in the postmenopausal period; A small proportion of 33 cases (12,5%) was postmenopausal. The rate of severe dysplasia according to colposcopic biopsy related with HPV types was; 58 (40%), 56 (30,8%), 18 (28%), 45 (27,3%), 31 (26,1%), 39 (25%), 59 (16,7%), 35 (14,3%), 51 (13,8%), 33 (12,5%), 16 (11,8%), 52 (8,3%). The prevalence of HPV types, the age at which they saw, the menopausal status and the potential for the formation of severe dysplasia are highly variable. We think that routine screening programme, colposcopy indications and vaccination program should cover all HPV types according to data.

Evaluation of the i-STAT Blood Gas Analysis System in Cardiovascular Surgery Çiğdem Unal Kantekin; Müjgan Ercan; Esra Firat Oğuz; Ertan Demirdaş; Kıvanç Atılgan; Mesut Sipahi; Ferit Çiçekçioğlu
Medical Laboratory Technology Journal Vol. 4 No. 2 (2018): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

The aim of this study was toinvestigate the compatibility of the parameters measured with the i-STAT blood gas analyser and the conventional blood gas analyser Rapid Point 500 (Siemens Healthcare Diagnostics, USA) in patients who underwent cardiovascular surgery. This clinical study included fifty patients undergoing coronary artery bypass surgery. Fifty whole blood samples were portioned and measured on the i-STAT and RP500 laboratory analyzers. The compatibility between pH, pCO2, pO2, Hb, Na+, K+, iCa2+ and glucose values was investigated.There was a good correlation of the i-STAT analyser with the RP500 analyser, with the exception Hb and Na+. Also all parameters except for Hb and ionized calcium were found to be within acceptable range in terms of clinical decision limits. It is very important that the point-of-care devices give accurate results as well as quick results. For this reason, we absolutely think that the point of care devices should be subjected to external and internal quality control programs, users should be trained regularly and feedback studies should be done.

Comparison Analysis of Total Cholesterol Level Examination Between Photometry and 3 Parameters Point of Care Testing Device Perdina Nursidika; Wikan Mahargyani; Fitri Kurnia Anggraeni
Medical Laboratory Technology Journal Vol. 4 No. 2 (2018): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Total cholesterol is the composition of many substances including cholesterol, triglycerides, LDL cholesterol, and HDL cholesterol. Cholesterol examination is one of the most frequent tests required in the laboratory to monitor vascular and cardiovascular diseases. Most clinical pathology laboratories use photometer to perform clinical chemistry checks. Cholesterol testing can also be done with Point of Care Testing (POCT) which has a working principle of biosensor technology. This research method is experimental, using 40 samples that can represent normal and pathological levels. All samples will be checked for total cholesterol with a photometer of CHOD-PAP method and 3 POCT Lipid Pro. The results showed linear regression y = 0.955x + 1.8325 with R2 of 0.9955. The linear regression value is calculated by Total Error (TE), while the Total Error Allowable (TEa) cholesterol is 10%. The bias value is 0.31%, TE for normal level = 5.92% and TE for high pathological level = 3.00%, it can be stated the result of examination can be compared or accepted. The% TE value obtained is less than the TEa value of cholesterol. It can be concluded that the total cholesterol results examined by the photometer and LipidPro are comparable. For further research it is advisable to use a total cholesterol sample that has a value of more than 400 mg/dL.

Study of Culture and Sensitivity Pattern In Urinary Tract Infections in A Tertiary Care Center in Nepal Binod Chapagain; Parshal Bhandari; Binod Aryal
Medical Laboratory Technology Journal Vol. 4 No. 2 (2018): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

The main purpose of this study was to find out the causative agents of urinary tract infections (UTI) and their culture and antibiotic sensitivity in patients visiting Tribhuvan University and Teaching Hospital (TUTH). A retrospective study conducted among 155 patients, aged from 25-50 years with culture-positive UTI, who visited TUTH from 1st April 2017 to 30th September 2017. A culture of midstream urine was done to find out causative agents and their antibiotic sensitivity performed. Data were evaluated using Microsoft Excel 2016. Female were more affected than males. Escherichia coli (E. coli) was the most common microbes causing UTI in 53% patients. Most of the isolates on culture were Multi-Drug Resistant (MDR) strains to comprise 52%. Of the total gram-negative organisms, 33.9% were Extended Spectrum β-lactamase (ESBL) producers, and 3.57% were Metallo β-lactamase (MBL) producers. 29.41% of Staphylococcus were resistant to methicillin. E.coli is the most common organism causing UTI among adults. Multidrug-resistant has appeared alarming with resistant to most of the first line antibiotics.

Detection of Salmonella typhi Using Multiplex and Monoplex PCR in Tifoid Fever Patients Andika Aliviameita; Meryn Linanda Agustin; Anggie Wedha Puspita; Miftahul Mushlih; Puspitasari Puspitasari; Yanik Purwanti; Arief Wisaksono
Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Typhoid fever is a disease caused by Salmonella typhi infection. This disease is still a global problem. The purpose of this study was to develop a rapid detection method for Salmonella using molecular methods utilizing Inv A and Fim-C Genes. Two methods compared, which are Multiplex and monoplex PCR. The sample is in the form of the patient's blood, which is stored at 3 ml EDTA vacutainer tube consisting of 10 positive samples and five negative samples. The criteria for samples used previously have tested by widal/ immunoserology (titer more than 1/160). The results showed that multiplex PCR for detection of Salmonella using the Inv A gene and fim c gene not recommended because too many bands produced. The application of monoplex in the Inv A gene gives better results than the Fim C gene. In conclusion, the monoplex application on Inv A gene recommended than Fim C gene used to detect S. typhi in human blood samples.

Association of the TG/HDL-C and Monocyte Count/HDL-C Ratios with Coronary Artery Disease in Non-ST Segment Elevation Myocardial Infarction Uğur Küçük; Recep Kurt
Medical Laboratory Technology Journal Vol. 5 No. 1 (2019): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Monocyte Count/HDL ratio (MHR) is a simple and inexpensive parameter used as a risk predictor in patients diagnosed with myocardial infarction (MI). This study aims to investigate the association between Triglyceride/HDL-C (TG/HDL-C) ratio and coronary artery disease in patients diagnosed with non-ST segment elevation MI (NSTEMI). Total of 100 patients, 50 patients with NSTEMI and 50 healthy individuals, who had admitted to the cardiology outpatient clinic between January 2018 and January 2019 included in the study. Higher monocyte count, MHR, and TG/HDL-C ratios detected in patients with coronary artery disease in comparison to healthy individuals. Although monocyte count was higher in NSTEMI group, the difference was not statistically significant (p=0.101). There was an increase in the MHR NSTEMI group when compared against the control group (p

Analysis between Adrenal MRI Mass Size and 1 mg Dexamethasone Suppression Test in Patients with Adrenal Incidentaloma Pinar Karakaya; Bulent Yaprak; Bahar Ozdemir; Hakan Kocoglu
Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

This study aimed to investigate the correlation of adrenal magnetic resonance imaging (MRI) mass size and 1 mg dexamethasone suppression test results in patients with adrenal incidentaloma diagnosis. Eighty-six patients (82.6% female) with incidentally discovered adrenal masses, diagnosed and treated in the Department of Endocrinology Bakirkoy Dr. Sadi Konuk Education and Research Hospital between June and August 2017, were included in the analysis. All the patients evaluated for their hormonal functionality. 24-h urinary excretion of cortisol did not correlate with tumor size. But there was a positive correlation between longer adrenal nodule diameter and metanephrine, and there was a negative correlation between shorter adrenal nodule diameter and renin levels. We found that smaller and clinically “silent” tumors often do not demonstrate subclinical hormonal activity.

Relationship Between Gender, Age, Duration And Frequency Of Hemodialysis Therapy With The Creatinine Level reduction Of Pre And Post Hemodialysis Anny Thuraidah; Misbawati Misbawati; Nurlailah Nurlailah; Haitami Haitami
Medical Laboratory Technology Journal Vol. 5 No. 1 (2019): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

The kidneys are organs of the body that function to regulate water balance in the body, the concentration of electrolytes in the blood and acid-base balance and secretion of waste material. If the kidney fails to function, the patient will need immediate treatment and even undergo hemodialysis (HD) therapy. A critical indicator in determining whether a person with impaired kidney function requires HD therapy is to know the creatinine level. The study aimed to ascertain the differences in creatinine levels pre and post HD also study the relationship between the age, gender, duration and frequency of HD therapy of respondents with the decrease of creatinine levels pre and post. Type of research detailed survey with a cross-sectional design. The sample was taken using a total sampling technique of 35 respondents from H BadaruddinKasimHospitalin Tanjung with a sample examination technique using the Jaffe method. The examination results of creatinine levels average for pre and post hemodialysis was11,36 and 5,58 mg/dl, which decreased 51%. The analysis statistically used Paired T-Test has a p-value = 0,000, indicating a significant difference for creatinine levels pre and post HD. Relationship between Age, Gender, Duration, and Frequency of HD Therapy with the decrease of creatinine levels pre and post HD was analyzed with Spearmen correlation and had p values more than 0,05. It means there was no relation between them. Conclusion there were significant differences in creatinine levels reduction pre and post HD while the relationship between the four of respondent characteristics to the magnitude of creatinine reduction show that there was no significant relationship.

Analysis between Platelet Count and Blood Groups in Apheresis Platelet Donors with Demographic Features Canan Eren; Serpil Çeçen
Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Platelet transfusion is used to prevent bleeding in patients with thrombocytopenia or platelet dysfunction. Purpose of investigating demographic characteristics of eligible volunteers as platelet donors and of demonstrating the association of platelet counts with blood groups as well as other factors. We used data of individuals who referred to a blood center in our hospital. Age, body weight, body mass index (BMI), and gender were determined, and than hemogram values such as leukocyte, hemoglobin, hematocrit, and platelet and ABO blood types of those individuals were identified. A statistically significant difference determined for height, body weight, and BMI in both genders. Although BMI was lower in the group of platelet count ≤ 250, it was higher in a group of platelet count > 250. Furthermore, platelet count was more moderate in blood group O Rh-positive but, no significant difference was group O Rh-negative. Platelet count was higher in other Rh-positive blood groups than Rh negatives. BMI is vital in apheresis donors, and individuals with higher BMI values may prefer in case of double dose or more apheresis need. The determination of platelet counts in Rh positives is more elevated than Rh negatives.

Page 6 of 20 | Total Record : 193

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Ricko Dharmadi Utama

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Jl. H. Mistar Cokrokusumo No.1A, Kemuning, Kec. Banjarbaru Selatan, Kota Banjar Baru, Kalimantan Selatan 70714

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Ricko Dharmadi Utama

Contact Email apinaga53@gmail.com

Phone +6282256602225

Journal Mail Official editormltj@gmail.com

Editorial Address Jl. H. Mistar Cokrokusumo No.1A, Kemuning, Kec. Banjarbaru Selatan, Kota Banjar Baru, Kalimantan Selatan 70714

Location Kota banjarmasin, Kalimantan selatan INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Ricko Dharmadi Utama

Contact Email
apinaga53@gmail.com

Phone
+6282256602225

Journal Mail Official
editormltj@gmail.com

Editorial Address
Jl. H. Mistar Cokrokusumo No.1A, Kemuning, Kec. Banjarbaru Selatan, Kota Banjar Baru, Kalimantan Selatan 70714

Location
Kota banjarmasin,

Kalimantan selatan

INDONESIA