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INDONESIA
Jurnal Biodjati
Published by Universitas Islam Negeri Sunan Gunung Djati Bandung
ISSN : 25481606     EISSN : 25414208     DOI : 10.15575/biodjati
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences
Arjuna Subject : -
Articles 482 Documents
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Isolation and Detection of Bacteriocin-Like Inhibitory Substances-Producing Bacteria from Fermented Mare’s Milk Sumbawa Alifia Issabella Mulyawati; Tri Ardyati; Yoga Dwi Jatmiko
Jurnal Biodjati Vol 4, No 1 (2019): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i1.4194

Abstract

  Bacteriocin-like inhibitory substances (BLIS) produced by bacteria is a promising future food preservative agent. This study aimed to obtain bacterial strains that can produce broad-spectrum antibacterial agents and identify the best BLIS producer species based on 16S rDNA sequences. The bacterial strains were isolated from fer-mented mare’s milk using MRS and M17 agar medium. The isolates then were initially screened based on its antibacterial activity of crude cells against Staphylococcus aureus ATCC 6538. The selected strains were cultured and harvested for its cell-free supernatant (CFS). The pH of CFS was adjusted to 6.5 then used for antibacterial activity as-says against ten pathogenic bacteria. Also, the proteinaceous nature of BLIS compound was confirmed by testing with proteinase K. The gDNA of selected isolates was extracted and the 16S rDNA was am-plified using the polymerase chain reaction method then sequenced. The 16S rDNA sequences of the selected strains were used to identify the species using BLAST nucleotides from NCBI then the phylogenetic trees were constructed. 32 isolates was obtained, but only three iso-lates (BC9, SB7, and DC12) were selected as a result of antibacterial screening for further assays. The neutralized-CFS (N-CFS) of these isolates exhibited broad-spectrum antibacterial activity. The N-CFS could be assumed as BLIS. The isolate of BC9 was identified as Ba-cillus amyloliquefaciens strain BC9 that has 99.99 % similarity with B. amyloliquefaciens KC-1, SB7 was Lactobacillus plantarum strain SB7 that has 99.99 % similarity with Lb. plantarum JMC 1149T, and DC12 was Lactobacillus rhamnosus strain DC12 that has 100 % sim-ilarity with Lb. rhamnosus DSM 20021T. Thus, the BLIS produced by those strains is potential for future food and beverages preservations. 
Immunoinformatics Study on Early 4 Protein of Human Papillomavirus Type 16 for Cervical Cancer Vaccine Peptide Candidate Yani Suryani; Opik Taupiqurrohman; Muhammad Yusuf; Toto Subroto; Sukma Nuswantara
Jurnal Biodjati Vol 4, No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.5414

Abstract

 The aims of this study were to carry out testing of the early 4 protein of type 16 HPV through immunoinformatics meth-ods in an effort to get the peptide vaccine candidate for cervical cancer. The software used are IEDB-AR, CABSdock and Accelrys Discovery Study 4.5. Based on the analysis that sequence of ami-no acid lysine, leucine, leucine, glycine, serine, threonine, tryp-tophan, proline and threonine (KLLGSTWPT) and the sequence of amino acid tyrosine, tyrosine, valine, leucine, histidine, leucine, cysteine, leucine, alanine, alanine, threonine, lysine, tyrosine, pro-line and leucine (YYVLHLCLAATKYPL) are peptide vaccine can-didate for cervical cancer from the early 4 protein of HPV type 16 
The Diversity and Diameter Growth Rate of Sprouting-Type Tree in Bukit Pinang-Pinang Forest Permanent Plots Ade Ayu Oksari; Irvan Fadli Wanda; Erizal Mukhtar; Chairul Chairul
Jurnal Biodjati Vol 4, No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.4728

Abstract

The process of forest regeneration can take place within a certain period of time. Sprouting is a form of strategy for in adapting to its environment for success in regenerating. The Research on  the diversity and diameter growth rate of sprouting-type tree in Bukit Pinang-pinang forest permanent plots, West Sumatra was carried out from October 2012 to December 2016 in the forest of Bukit Pinang-Pinang, West Sumatra and Herbarium Department of Biology, Universitas Andalas, Padang. This research was conducted using a survey method by census. The parameters analyzed were plant species composition, Morishita Index, Mann-Whitney test, Regression and Correlation test as well as RDGR (Relative Diameter of Growth Rate). The result of experiment was found 40 species of trees sprouting type in forest permanent plot Bukit Pinang-Pinang. The pattern of sprouting type trees distribution in the study locations was found to have a clustered, random and uniform pattern. Relative Diameter Growth Rate (RDGR) of 40 sprouting types of trees in the Bukit Pinang-Pinang forest permanent plots ranged from 0.003 cm/cm/year to 0.0288 cm/cm/year. Altitude did not affect  RDGR of an individual tree sprouting type for 31 years
Effect of Pectin Biodegradation with Aspergillus niger on Total Flavonoid Content of Citrus limon L.) Muhammad Yusuf Abduh; Wahyu Zuliansyah; Lestari Aprina; Natalie Arazella
Jurnal Biodjati Vol 4, No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.4537

Abstract

Lemon peel and leaf  as well as lower grade of lemon contain a considerable amount of flavonoid which can be extracted to produce a valuable product. The presence of pectin in lemon hinders the extraction of flavonoid compound. This research was conducted to examine the effect of pectin biodegradation of lemon leaf, peel, and lower grade fruit using solid-state fermentation towards the yield of pectin, crude flavonoid, and total flavonoid content. Fermentation was carried out with the help of Aspergillus niger at 30℃ for 3, 5, 7, and 9 days of cultivation time. Pectin was extracted from the fermentation broth using citric acid. Crude flavonoid was extracted using maceration and stirred about 500 rpm at 50℃ for 40 minutes. Total flavonoid content was analyzed using a spectrophotometer. The optimum cultivation time of Aspergillus niger with leaf and peel was 9 days with a pectin yield from lemon leaf was 0.43% dry weight and the pectin yield from lemon peel was 0.04% dry weight. As for the lower grade fruit, a cultivation time for 7 days showed an optimal result with a pectin yield of 0.08% dry weight. The total flavonoid content in the leaf increased up to 94.3% (1.06 mg of the quercetin equivalents per g of substrate) whereas the total flavonoid content in the peel and lower grade fruit increased up to 42% (0.12 mg of the quercetin equivalents per g of substrate) and 48% (0.021 mg of the quercetin equivalents per g of substrate), respectively. These results highlighted the potential valorization of Citrus limon L. as an alternative source for producing flavonoid in food and pharmaceutical industry.  
Growth of Slipper Orchid Paphiopedilum javanicum (Reinw. ex Lindl.) Pfitzer during Acclimatization Stage Ema Hendriyani; Tri Warseno; Gebby Agnessya Esa Oktavia
Jurnal Biodjati Vol 4, No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.5665

Abstract

 Paphiopedilum javanicum (Reinw. ex Lindl.) Pfitzer known as slipper orchid is an endemic terrestrial orchid from Indonesia. This orchid is threatened with extinction and listed under CITES Appendix I. In-vitro propagation of P. javanicum has been done in Bali Botanic Garden (BBG) for last six years but acclimatization stage has not given satisfied result. The purpose of this study was to know the ef-fect of liquid fertilizer on vegetative growth of P. javanicum planlet during acclimatization stage. Beyonic StarTmik liquid fertilizer ap-plied by five different doses considered as treatment of 0, 10, 20, 30 and 40 mL. They were 10 planlets for each treatment considered as replication. In this research we observe vegetative growth of P. ja-vanicum by quantitative and qualitative parameter. Quantitative data were analysis by ANOVA. Result showed, high percentage of planlet survival was observed of 98% and liquid fertilizer dose for 30 mL gave the optimal vegetative growth of P. javanicum planlet with aver-age height of 2.2 cm and leaves number of 13 pieces. There were no differences on qualitative parameter in all treatment that all leaves color was dark green and leaves tessellation appearance was obvious 
Genetic Relationship of the Transgenic and Non-Transgenic Mutiara Catfish with their Hybrids Sangkuriang Catfish Iskarimah Yolanisa; Ibnu Dwi Buwono; Yuniar Mulyani; Iskandar Iskandar
Jurnal Biodjati Vol 4, No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.4741

Abstract

The male broodstock of Transgenic Mutiara catfish (P) (containing Clarias gariepinus Growth Hormone, CgGH) is a fish who show fast growth. Propagation of the Transgenic Mutiara catfish F1 and F2 progeny involves the crossing of broodstock P with Non Transgenic Mutiara catfish. New genetic variations (polymorphisms) can be generated from crosses broodstock P and F1 which lead to the fast growth inheritance of transgenic fish. Phylogenetic relationship analysis showed distance and genetic variation in broodstock P, F1, F2 and Sangkuriang catfish (as controls) using the RAPD-PCR method (Random Amplified Polymorphic DNA-Polymerase Chain Reaction) as the research purposes. The results showed that OPA-03 (5'-AGTCAGCCAC-3 ') primer was selective for determining phylogenetic among catfish samples. The closest genetic similarity index originates from the female broodstock of Non Transgenic Mutiara catfish (P) (93%), while the farthest originates from the male broodstock of Mutiara Transgenic catfish (P) (51%) with F1 dan F2. Closely related broodstock catfish is not recommended to be crossed.
Anatomical Structure of Sepal and Petal of Red Dragon Fruit (Hylocereus polyrhizus Britton & Rose) During Flower Development Utaminingsih Utaminingsih; Sulhan Etfanti; Suharyanto Suharyanto; Maryani Maryani
Jurnal Biodjati Vol 4, No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.4581

Abstract

Red dragon fruit (Hylocereus polyrhizus Britton & Rose) is one type of cactus plant which is very potential as an ornamental plant and edible fruit. Flower is organ that play an important role in the process of breeding plants generatively. This reasearch aimed to study the anatomical structure of sepals and petals of red dragon fruit plants during flower development. The research stages included: sepals and petals sampling that held at various stages of flowering ; morphological observation (measurement length of sepals and petals); anatomical slides of sepals and petals cross section using the embedding method, anatomical observation and image capture of sepals and petals. The parameters observed were bud size, sepal length, petal length, sepal thickness, petal thickness, and tissue description composed. The results of this study indicated that buds have an increased development pattern. The increase in bud size is directly proportional to the stage of the bud. Sepal and petal are composed of epidermal tissue which form papillae, cryptophore stomata, secretory parenchyma space containing mucus, and tissues transport system is closed collateral.
Community Structure of Fish and Environmental Characteristics in Cisadea River, West Java, Indonesia Epa Paujiah; Dedy Duryadi Solihin; Ridwan Affandi
Jurnal Biodjati Vol 4, No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.6096

Abstract

 Environmental characteristics can influence the distribu-tion of animal communities including fish in aquatic habitats such as a river. The study of fish communities in Cisadea River aimed to learn the relation between the fish community structure and the environmental characteristics of the river itself. This research was conducted in Cisadea River, Cianjur Regency, West Java, Indone-sia during dry season from July 2012 until February 2013. Samples of fish were caught using active fishing gear (Electrofishing Gear, spreading nets, scoop, lift net) and passive fishing gear (trap). The results showed that the quality of the physical chemistry of Cisadea waters was still in the normal range that could support fish life. The fish community in Cisadea River consisted of 11 orders, 26 families, 35 genera and 48 species. During the study, Rhyacichthys aspro (19,85%) and Glyptothorax plathypogon (20,15%) were the domi-nant species caught. The diversity index value (H’) was 2.87 (mid-dle category) with the dominance value of 0.09 (low category) and evenness value of 0.74 (high category). Moreover, community simi-larities according to the presence of species made the LC1 and LC2 locations in one group while the LC3 locations in a separate group. 
Nine Additional Cultivated Species of Asteraceae from Java Arifin Surya Dwipa Irsyam; Rina Ratnasih Irwanto
Jurnal Biodjati Vol 4, No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.4815

Abstract

Asteraceae is one of the largest families in the Angiosperm. It comprises of 24,000‒30,000 species which are grouped into 1,600‒1,700 genera and distributed in a cosmopolitan range. Based on Backer and Bakhuizen van den Brink’s 1965 works, they identified 227 species of Asteraceae in Java. Many of them are introduced species, both cultivated or naturalized. However, the cultivated species have not been recorded. The aim of this study is to provide information about new records of cultivated Asteraceae in Java. The observations were carried out in Bandung, Bogor, and Sumedang, during January to February 2019. Nine additional species were discovered; species belonging to genera Caputia (two species), Curio (two species), Kleinia (one species), Gymnanthemum (one species), Senecio (two species), and Tarlmounia (one species). These species were grouped into two tribes: Vernonieae and Senecioneae. This study indicates that information on Asteraceae of Java need to be updated.
Growth and Flavonoid Content of Gynura procumbens (Lour) Merr. in Different Soil Water Content Evika Sandi Savitri; Farrikhatun Khusnia
Jurnal Biodjati Vol 4, No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.4446

Abstract

Gynura procumbens contains tannins, saponins, steroids, triterpenoids, essential oils and flavonoids which have bioactivity as a medicine for various diseases. One effort to obtain the quality of medicinal plants uses management of soil water content. The opti-mum of soil water content will support optimal growth of a plant. Whereas in conditions of low soil water content resulted in the induc-tion of the production of secondary metabolites as a self-defense sys-tem. This study aimed to determine the effect of soil moisture content on the growth and total content of plant flavonoids G. procumbens. This study was carried out experimentally using a one-factor com-plete randomized design (CRD) consisting of 4 treatments with five replications. The treatment used is the difference in soil water content of 40%, 60%, 80% and 100% field capacity. The data obtained in the form of quantitative data will be analyzed by One Way ANOVA Test. Measurement of the content of total flavonoid compounds was car-ried out using UV-Vis spectrophotometer analysis. The results of the study indicate the influence of soil moisture level on the growth and total content of G. procumbens. Parameter growth of fresh weight, dry weight, and root G. procumbens showed an increase in 40% soil water content. The flavonoid content showed the highest total flavo-noid content 18.884 mg/g in the 40% field capacity soil water content 

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